Objective To explore a simple, effective and stable method for the isolation and purification of Kupffer cells from rat liver, enabling further study on the structure and function of these cells in vitro. Methods After laparotomy, a catheter was inserted into the portal vein and secured with artery clamp. Then, the rat liver was perfused and digested with solution Ⅰ and solution Ⅱ containing 0.05% collagenase Ⅳ respectively. The cell suspension was centrifuged with isopycnic sedimentation in a two-step Percoll gradient to harvest Kupffer cells. The isolated Kupffer cells were purified by selective adherence after 30 min of cultivation, and identified by evaluation of phagocytosis of India ink and peroxidase staining with DAB through light and electron microscopy. Results It was verified that the viability of isolated Kupfffer cells was more than 90% through Trypan blue staining. Those Kupffer cells could attach to plastic quickly and phagocytose ink, and had the appearance of "fried eggs" in positive peroxidase staining with a purity of 95%. Under the light microscopy, the appearance of newly isolated Kupffer cells was round with uniform shape and size. After two days of culture, Kupffer cells appeared to distend with irregular or stellate shape. The typical features were observed in the transmission electron micrographs. There were numerous pseudopods and occasional cup-like indentations in the cell membrane of Kupffer cells. The cytoplasm contained numerous types of lysosomes and other phagocytotic vesicles. Conclusion The method for isolating and culturing Kupffer cells in this study is effective and stable, and the biological characters are preserved in the cultured cells.

Overnutrition during the early postnatal life,a critical time window for growth and development,may induce metabolic syndrome later in life,including overweight/obesity and insulin resistance.The important target organs of insulin,such as liver,adipose tissue,skeletal muscle,and central nervous systems show insulin resistance.The involved mechanisms include abnormality of insulin signal pathway,increment of free fatty acid and some adipocytokines,oxidative stress,maladjustment of orexigenic and anorexigenic neuron,modifications of the hypothalamic-pituitary-adrenal glucocorticoid axis as well as epigenetic,etc.Hence,overnutrition should be avoided during the early postnatal life,so as to decrease the risk of developing long-term insulin resistance.

This article reviewed hot spot and problems in the research of the senile dementia,and proposal the researching thoughts and the tentative treament plan of this disease.

Objective: To introduce recent research progress in anti-diarrhea with single Chinese herb and its effective compo-nents. Methods:The references on anti-diarrhea with single Chinese herb and its effective components in recent years were reviewed and analyzed. Results:The researches on anti-diarrhea activity and possible mechanism of single Chinese herbs, such as Terminalia chebula Retz, Fructus mume, Radix Puerariae and Pulsatilla chinensis ( Bunge) Regel, were reviewed. Conclusion: At present, the researches on anti-diarrhoea with single Chinese herb and its effective components have made some progress, while further and wider researches are still needed to explore the underlying mechanisms.

Objective To investigate the relationship between serum uric acid level and the severity of pregnancy induced hypertension (PIH) in pregnant women with hypertension.MethodsFrom January 2016 to July 2016 in our hospital for treatment of 100 cases of patients with pregnancy induced hypertension, as PIH group, divided into severe hypertension group (33 cases), moderate PIH group (33 cases) and mild PIH group (34 cases), then select 100 cases of normal pregnant women normal pregnancy group.The serum uric acid levels were measured and the results were analyzed.ResultsThe average serum uric acid levels in PIH group (359.75+89.23) mol/L was significantly higher than the control group (188.24+54.54) mol/L in PIH group, the average level of serum uric acid in severe, moderate and mild degree is decreasing, respectively (449.32+81.11), (352.89+76.41), (288.76+59.58) mol/L, a statistically significant difference between the groups (P<0.05).ConclusionThe detection of serum uric acid level in pregnant women can effectively determine the degree of pregnancy induced hypertension in pregnant.

Animals ; Antioxidants ; pharmacology ; Cerebellum ; cytology ; Dietary Supplements ; Drugs, Chinese Herbal ; chemistry ; Fruit ; chemistry ; Male ; Muscle Fatigue ; drug effects ; Neurons ; drug effects ; pathology ; Oxidative Stress ; drug effects ; Physical Exertion ; Rats ; Rats, Wistar ; Vegetables ; chemistry

ObjectiveTo research in the essence of the caring experience of caregiver for children with eye enucleation, to find out the caring experience and caregiver's needs, to give them physical and mental nursing in order to make them cooperate with us and implement effective treatment to children.Methods12 caregivers for children with eye enucleation were given semi-structured interviews. The materials were analyzed, putted in order, and themes were got.ResultsThrough deep interview of 12 caregivers, five titles were summarized:overburdened psychology, limited schedule, requirement of health information, overload economic burden, to return and rebuild.ConclusionsThe results could help nurses to give more correct health education and psychological care to caregivers. Then caregivers can cooperate with nurses to give effective treatment to children, thus life quality of children with eye enucleation will be improved a lot.

Quality indicators (QIs)in laboratory medicine can measure how well a clinical laboratory meets the needs and re-quirements of users and the quality of all operational processes.The identification of reliable QIs is a crucial step in enabling users to quantify the quality of laboratory services.Different QIs and terminologies are currently used and,therefore,there is the need to harmonize proposed QIs.A model of quality indicators has been consensually developed by a group of clinical la-boratories according to a project launched by a working group of the International Federation of Clinical Chemistry and La-boratory Medicine.The model includes 57 QIs related to key processes (35 pre-,7 intra-and 15 post-examination phases)and 3 to support processes.The developed a model of quality indicators and the data collected provide evidence of the feasibility of the project to harmonize currently available QIs,but further efforts should be done to involve more clinical laboratories and to collect a more consistent amount of data.

Myelodysplastic syndromes (MDS) is a heterogeneous group of clone bone marrow disorders.The diagnosis of MDS can be challenging when dysplastic features are minimal and cytogenetic analysis shows no abnormalities The high sensitivity of flow cytometry (FCM) in the detection of dysplasia in myeloid lineages makes it a valuable tool to distinguish possible clonal causes of cytopenia(s) from nonclonal causes.Due to the heterogeneity and absence of specific FCM markers for MDS,the immunophenotyping of MDS by FCM remains a big difficulty.Here we will focus on how to perform immunophenotyping of MDS by FCM and the potential diagnostic,prognostic and therapeutic value of FCM in MDS.

Objective To compare and analyze the clinical effects and reliabilities of intermandibular flexible distraction and rigid internal fixation in treating mandibular condylar fractures. Methods Of 40 patients with mandibular condylar fractures, 21 cases were managed with intermandibular flexible distraction (external fixation group, Group A) and 19 with rigid titanium internal fixation (internal fixation group, Group B). There were 15 cases with high position fractures and 25 with low position ones. Results During 2-4 weeks postoperatively, X-rays confirmed the condylar fracture healing in all cases with no loosened mini-tinum plates in the Group B. There were 10 cases in the Group A and 14 cases in the Group B with the opening width over 30 mm, and 11 cases in the Group A and 5 in the Group B with the opening width less than 30 mm. Conclusions Both external and internal fixations can attain condylar fracture healing. However, compared with the internal fixation, the reduction of external fixation is less exact and the function recovery slower after operation, especially in the low position fractures. No irreversible damages on TMJ happen in all cases.