Objective:To construct a vector containing human vascular endothelial growth factor,VEGF 121 (VEGF 121 ) with green fluorescence protein as the reporter gene,and then to observe its expression in osteoblast.Methods:VEGF 121 gene was amplified from foetus brain library by PCR,then ligated with pEGFP-C1 to form recombinant plasmid pEGFP-VEGF and transfected into osteoblast by liposome.The green fluorescence protein expression was observed by fluorescence microscopy and the VEGF expression was last detected by immunocytochemistry and ELISA.Results:VEGF 121 gene fragment was amplified by PCR and ligated to pEGFP-C1,then transfected into osteoblast successfully.The strong expression of green fluorecensce protein was observed by fluorecensce microscopy,and the expression of VEGF protein was detected by immunocytochemistry and ELISA.Conclusion:Recombinant plasmid pEGFP-VEGF is effectivly expressed after being transfected into osteoblast in vitro,which provides theoretical support for further study with its simple and reliable detecting methods.

Adaptor Proteins, Signal Transducing ; genetics ; Bone Marrow Diseases ; genetics ; pathology ; DNA Mutational Analysis ; DNA-Binding Proteins ; genetics ; Exocrine Pancreatic Insufficiency ; genetics ; pathology ; Glucose-6-Phosphatase ; genetics ; Humans ; Leukocyte Elastase ; genetics ; Lipomatosis ; genetics ; pathology ; Mutation ; Neutropenia ; congenital ; genetics ; Proteins ; genetics ; Transcription Factors ; genetics

Child ; Complement C3 ; metabolism ; Cytokinins ; analysis ; metabolism ; Female ; Humans ; Immunoglobulin A ; immunology ; Immunoglobulin G ; immunology ; Interferon-gamma ; immunology ; Interleukin-12 ; analysis ; immunology ; Interleukin-2 ; analysis ; immunology ; Male ; Peptide Fragments ; immunology ; Respiratory Tract Infections ; epidemiology ; immunology ; virology ; Secondary Prevention ; Tuberculin ; analysis

Reforming the managerial system and the educational system of higher medical education is the requirement of the social development in the twenty-first century. The article expounds the fundamental characteristics of medical education including small scale, longer-duration program, and high expenditure. It emphasizes the significance of following the rule of medical education, and analyzes the important position of medical college and the relation of medical college to affiliated hospitals and other related colleges in the comprehensive university. Based on the requirement of the social development and the trend of higher medical education internationalization, it also analyzes the necessity of the adjustment on the medical educational system and the way of the adjustment.

Professional education of nursing is an important means to bring up practical type of nursing talents.Combined with the existing problems in nursing profession's anatomy teaching of our college,we put forward some thoughts on modifying nursing anatomy course.

Objective To study the calculating model of military medical equipment readiness rate and the main affecting factors in order to make a valuable reference to the decision -making of medical organization for the further medical support. Methods Based on analysis of the problems in calculating method of military medical equipment readiness rate at present, a new improved calculating model and the analysis method relating to assigning mission was put forward by combining the technical requirements of GJB5338-2005 and the actual of medical equipment management. The factors affecting readiness rate in process of manufacturing, operation and maintenance support were analyzed. Results A new improved calculating model of military medical equipment readiness rate was established and the evaluating method was made by adjusting those correlated parameters in the calculation formulas. Conclusion The solution of the model is simple and convenient and the analyzing results show that the readiness rate is close to the reality. It is a practical method for achieving quality state information of medical equipment, so it can meet the requirements of military medical equipment organization in peacetime.

Objective To explore the relation between serum Hcy,Cys C levels and type2 diabetic macro-vascular complications and its clinical significance.Methods The serum levels of Hcy and Cys C were measured with immunoturbidimetry in 80 cases of type 2 diabetic patients with macrovascular complications and 80 cases of type 2 diabetes without macrovascular complications,and in 80 healthy subjects which was seleted as control.Results Serum levels of Hcy and Cys C of type 2 diabetic patients with macrovascular complications were (26.42 ± 5.75)μmol/L and (2.30 ±0.40)μmol/L,which were higher than those of patients without macrovascular complica-tions for (15.31 ±3.13)μmol/L and (1.51 ±0.27)μmol/L(t =15.17,14.64,all P <0.05);Moreover,which were also higher than those in the healthy control group for (8.86 ±1.62)μmol/L and (0.85 ±0.14)μmol/L(t =26.29, 30.56,all P <0.05).The serum levels of Hcy and Cys C of type 2 diabetic patients with carotid arteries increased were (32.53 ±6.46)μmol/L and (2.83 ±0.52)μmol/L,which were higher than those of patients without carotid arteries for (18.46 ±3.63)μmol/L and (1.65 ±0.30)μmol/L(t =11.64,12.06,all P <0.05).In patients of type 2 diabetic with macrovascular complications,Hcy was positively correlated with serum Cys C(r =0.617,P <0.05). Conclusion sCD40L and YKL -40 may have important role in the pathogenesis of type 2 diabetic patients with ma-crovascular complications,which can be used as a valuable reference target in the diagnosis and monitoring of vascular complications in elderly type 2 diabetes,and it may be one of the important independent risk factors of patients with type 2 diabetes.

A high incidence of esophageal cancer exists in China. Surgical resection remains the dominant therapeutic intervention for patients with operable esophageal carcinoma. However, alternative strategies are actively applied to reduce the frequency of post-op-erative local or distant disease recurrence and to prolong survival. These strategies include neoadjuvant and adjuvant therapies. This review discusses the current knowledge, as well as available data and information, with regard to neoadjuvant and adjuvant therapies for patients with locally advanced esophageal cancer.

Objective: To study characteristics of children's EEG complexity at different ages and in different physiological states.Methods: The continuous 24 hour EEG recordings were obtained from 16 electrodes in 45 essential healthy children between the ages of 0 to 15 years. EEG complexity was analyzed by non linear measure in 7 states: awake with eyes opened, awake with eyes closed, NREM (nonrapid eye movements) sleep including stages ⅠandⅡ (light sleep), ⅢandⅣ(deep sleep) and REM (rapid eye movements) sleep. Meanwhile, the correlation was analyzed between complexity and ages.Results: (1)The global EEG complexity in state of being awake with eyes opened was greater than that with eyes closed;that in wakefulness state was greater than in sleep state. The EEG complexity gradually decreased with the increase of deep sleep in NREM sleep state.The complexity in REM sleep state was greater than that in deep sleep state, but lower than in wakefulness state. (2) The global EEG complexity was positively related to ages in state of being awake with eyes opened, state of being awake with eyes closed, light sleep, and not related to ages in deep-sleep state and REM sleep state.(3)In every brain area EEG complexity was positively related to ages in state of being awake with eyes closed .In paracentral region EEG complexity was positively related to ages in states of being awake with eyes opened and light sleep.Conclusion: The EEG complexity was used to study the brain dynamical characteristics in different physiology states and the relationship between encephalic electric activity and brain development. It can be used as an objective index to evaluate the function and development of brain.

Objective To investigate the secondary metabolites of the South China Sea sponge Acanthella cavernosa. Methods The acetone extract of A. cavernosa was purified by repeated column chromatography on silica gel, sephadex LH-20, and high-performance liquid chromatography (HPLC). Structures of the extract were determined by spectroscopic analysis and comparison with previously reported data. Results and Conclusion A total of 7 compounds were isolated and their structures were identified as kalihinene X-Z (1 - 3), (R)-3,5-diethyl-5-((S,E)-2-ethylhex-3-en-1-yl)furan-2(5H)-one (4), methyl(2Z,6R,8S)-4,6-diethyl-3,6-epoxy-8-methyldodeca-2,4-dienoate (5), methyl(2Z,6R,8R,9E)-3,6-epoxy-4,6,8-triethyl-2,4,9-dodecatrienoate (6), and methyl(2Z,6R,8S)-3,6-epoxy-4,6,8-triethyldodeca-2,4-dienoate (7); and compounds 4 - 7 were identified from the genus Acanthalla for the first time.