OBJECTIVE:To establish an HPLC method for the determination of cefepime in human plasma.METHODS:The separation of sample was carried on a Diamonsil C18 column with the mobile phase consisted of 20 mmol?L-1 ammonium acetate(which was adjusted to pH=5.00 by acetic acid)-acetonitrile(90∶10) at a flow rate of 1.0 mL?min-1.The detection wavelength was set at 254 nm;the sample size was 20 ?L,and the column temperature was set at room temperature.RESULTS:The linear range of cefepime was 0.2～50.0 ?g?mL-1(r=0.999 6),with the lower limit of quantitation at 0.2 ?g?mL-1.The average methodological recovery was 99.16%(RSD=1.29%) and the average extraction recovery was 85.43%(RSD=2.37%).The inter-day RSD and intra-day RSD were all less than 10%.CONCLUSION:The method was simple,sensitive,rapid and accurate and can be used for the pharmacokinetic and bioavailability study of Cefepime.

Objective To evaluate the feasible hand hygiene intervention methods to improve health care workers’ (HCWs)hand hygiene compliance,and reduce healthcare-associated infection(HAI)rate.Methods The surgical department in a hospital was selected as intervention department,intervention department was conducted baseline investigation in January-February 2013,multimodel intervention measures were conducted between March 2013 and April 2014,post-intervention survey was performed by the same method as baseline survey in May-June 2014,hand hygiene of HCWs as well as occurrence of HAI between before and after intervention was compared respectively. Results After intervention,HCWs’hand hygiene compliance before and after patient contact both enhanced signifi-cantly;HCWs’hand hygiene compliance rate increased from 53.32% before intervention to 69.27% after interven-tion,the difference was significant(χ2= 26.19,P<0.001 ). HCWs’hand hygiene modes before patient contact:liquid soap plus running water before intervention(67.02% ),alcohol-based hand rub after intervention (61 .08% );HCWs’hand hygiene modes after patient contact :both liquid soap plus running water before and after intervention (86.40% and 70.41% respectively). Before intervention,hand dry method of HCWs before and after patient con-tact was by wiping with uniforms,after intervention was by wiping with paper towel. HAI in intervention depart-ment decreased from 3.08% before intervention to 2.00% after intervention,the difference was significant(χ2=7.76,P= 0.005).Conclusion Multimodel intervention measures in intervention department can effectively improve HCWs’hand hygiene compliance rate.

OBJECTIVE To observe the efficiency of new low-temperature sterilization method using(hydrogen) peroxide gas plasma sterilization system.METHODS The hydrogen peroxide gas plasma sterilization system of STERRAD 100S was monitored for 157 sterilization cycles.RESULTS From them 134 cycles were(completed,) 23 cycles were(cancelled.) All cycles were completed with negative results either in biological culture or in chemical indicators.CONCLUSIONS The STERRAD Sterilization System involves the combined use of hydrogen peroxide and low-temperature gas plasma to sterilize most medical devices and materials rapidly and safely without leaving any toxic residues.As nursing develops,the new sterilization system is efficient and convenient.However,it also has own limitations.Therefore,it is desirable to fully understand the normal procedure of system according to the operation instruction.

Objective To observe the effect of PKB gene silencing on the growth of gastric cancer cell line SGC-7901 in vitro.Methods Gene transfection technique was used to transfect AKt2 siRNA into gastric cancer cells.Akt2 expression was detected by RNAi technique,Akt2 protein level was detected by Western blot,and the change of cell cycle distribution and apoptosis of SGC-7901 cells were detected by flow-cytometry,SGC-7901 proliferation was measured by MTT method.Results After SGC-7901 cells transfected with Akt2 siRNA,the expression of protein level decreased obviously(P

Objective:Spectrophotometer is a device to be verified obligatory in hospital. This article introduced some techniques to avoid ordinary mistakes in measurement. Methods:We introduced principle and verification regulation of spectrophotometer. Such methods as parameter configuration, proper maintenance can reduce our mistakes. Results: This article analyzed some common problems in measurement and also offered solutions. Conclusion: In the measurements of spectrophotometers, because the operators have less spectrophotometer operation experience, they often get inaccurate data which lead to an error. Conclusion: To avoid these mistakes, strictly regulate operations in spectrophotometer’s verification and proper routine maintenance are necessary.

Objective:To investigate the effect of oxaliplatin inhibit the growth in glioma U 87 cells by regulating PI3K/Akt sig-nal pathway.Methods:The glioma U87 cells were cultivated in vitro ,using 0,20,40,80 μg/ml oxaliplatin treated U87 cells 24,36, 48,72 h respectively,MTT was used to detect cell proliferation.Using 40 μg/ml oxaliplatin treated U87 cells 48 h,flow cytometry was used to detect cell cycle and apoptosis .Cells apoptosis protein and PI 3K/Akt pathway protein expression after 40 μg/ml oxaliplatin treated U87 cells 48 h was detected by Western blot .Results:MTT assay showed that compared with the 0μg/ml treatment ,oxaliplatin treatment could significantly inhibited U 87 cell survival ( P<0.01 ) ,40μg/ml oxaliplatin treatment 48 h ,the survival inhibitory was the most obvious.U87 cell cycle was arrested in S phase after 40 μg/ml oxaliplatin treatment 48 h.After 40μg/ml oxaliplatin treatment 48 h,compared with the 0 μg/ml treatment,U87 cell apoptosis rate significantly increased (P<0.01).Western blot showed that after 40μg/ml oxaliplatin treatment , anti-apoptotic factor Bcl-2 expression had significant decreased , pro-apoptotic factors Bax , Cleaved-caspase3 protein expression had significantly increased (P<0.01).PI3K,p-Akt expression was significantly decreased (P<0.01),and Akt expression had no significant change in Akt pathway (P>0.05).Conclusion:Oxaliplatin may suppress U87 cell proliferation,ar-rest cell cycle,and promote apoptosis by inhibit PI3K/Akt signaling pathway.

Objective To find out the features and causes of the complications of multiple system organs failure (MSOF) in pregnancy and increase the level of its prevention and treatment. Methods A retrospective study was carried out on 13 cases with the complication of MSOF in pregnancy admitted between Feb 1992～ Jun 1999. Results There were two or more organs or systems function failure in the pregnancy. Four cases induced by infection, 5 cases induced by heart diseases, 3 cases induced by pregnancy induced hypertension, 1 case induced by amniotic fluid embolism. 12 of 13 were cured.1 was died. Among them one infant was died. Conclusions During the treatment of pregnancy with multiple system organs failure, it is important to control the causes and inducements. It is forcible to terminate pregnancy in time and choose a best style of delivery. It is better to keep watch on saving multiple system organs function.

Objective To study the effects of propofol pretreatment on S100? and neurosecretion enzyme (NSE) of brain tissues with global cerebral ischemia-reperfusion in rat, and to evaluate the effects of propofol in protection of brain. Methods 30 SD male rats were randomly divided into 3 groups: sham operation group (group A, n=10); single cerebral ischemia-reperfusion group (group B, n=10); propofol pretreatment at 2h before ischemia group (group C, n=10), in which propofol (100mg/kg) was given intraperitoneally (ip) before ischemia. 24h after ischemia-reperfusion, the neuroethology scores were recorded and evaluated, and S100? and NSE in rat brain were determined. Results The neuroethology scores of group A were higher than those of group B (P

Aim To study the effects and mechanism of Guanxinkang capsules on myocardial injury induced by isoproterenol in rats.Methods 30 male Wistar rats were randomly divided into 3 groups:control group,acute myocardial ischemia group and Guanxinkang capsules group.Rats were given injections of ISO subcutaneously.Electrocardiography were performed,serum and myocardium creatine kinase(CK),creatine kinase MBmass(CK-MB),lactate dehydrogenase(LDH) and nitric oxide(NO) were detected,and the levels of NO and endothelins(ET) in myocardium were measured as well.Results The rising degree of ST-T obviously decreased in Guanxinkang capsules group,and the levels of serum CK,CK-MB and LDH decreased in Guanxinkang capsules group,compared with those of acute myocardial ischemia group(P

Objective To explore the accurate position for the intersect between the short axis of the tibial tray and patellar ligament through studying the relationship among the femoral epicondylar(FE) axis, posterior femoral(PF) axis, posterior tibial(PT) axis, patellar(PAT) axis and patellar ligament(PL). Methods Magnetic resonance imaging (MRI) scans of 40 knees are included in this study. Using the computer workstation, these axial scans were used to construct the FE axis,PF axis, PT axis, PAT axis and PL. Angles between the axes of the selected landmarks were calculated, and linear regressions were performed to assess the relationship between the axes. Template of the tibial tray were positioned along both the FE axis and PT axis and the resultant points of patellar ligament intersection were recorded, and the difference of the intersect was analyzed with the matched t test. Results Strong relationship were found between the FE and PAT axes(1.8?? 3.6?, R2=0.70), as well as between the FE and PF axes(6.1?? 3.0?, R2=0.79). When the tibial template was aligned along the FE axis, 65% of the cases were in an ideal position, and the intersect is at the 10%-60% width of the patellar tendon. When the PT axis was used, 35% were optimal, and the intersect is at the -15%-35% of the width. And the difference in the mean value of the intersect is of statistical significance. Conclusion During total knee arthroplasty, if the short axis of the tibial tray is aligned with the intersects of the PL between its midpoint of the medial third to a half of its entire width, the rotation of the tibial component will fit the FE and PAT axes closely, so the component's position is appropriate.