Child ; Humans ; Kidney ; pathology ; Lymphoma, T-Cell, Cutaneous ; pathology ; Male ; Skin Neoplasms ; pathology

Objective To discuss the influence of Recipe Wenzhongxiaotan to S180 bearing cancer mouse interfered by cold pathogenic factor and study correlation factor index. Methods To treat S180 bearing cancer mouse by interfere in ice water with Recipe Wenzhongxiaotan, with the control of Recipe Xiaotansanjie group and chemotherapy group, the change of morphology and rectal temperature in mouse, growth velocity and volume in tumor after treatment with Recipe Wenzhongxiaotan were observed, and TXB2 in plasma of mouse was detected by RIA. Results It showed late and short in chilly morphology and rectal temperature amplitude small than other groups in Recipe Wenzhongxiaotan group. The rate to restrain tumor growth was 86.69%, 75.29%, 87.55% in Recipe Wenzhongxiaotan group, Recipe Xiaotansanjie group and chemotherapy group (FT-207), respectively. TXB2 was near in Recipe Xiaotansanjie group and chemotherapy group and the difference was significant compared cold model group (P 0.05) in Recipe Wenzhongxiaotan. Conclusions It may be restrain tumor growth which S180 tumor-bearing mice interfered by cold pathogenic factor and degrade TXB2 in Recipe Wenzhongxiaotan, the rate to restrain tumor growth was near to FT-207 and Recipe Xiaotansanjie.

OBJECTIVE: To observe the effects of warming spleen and stomach for dispersing phlegm recipe (Wenzhong Xiaotan Recipe, WZXTR), a compound traditional Chinese herbal medicine, on expression of interleukin-8 (IL-8) in S(180) tumor tissue in mice, and to explore the effectiveness and its mechanism. METHODS: Fifty mice were randomly divided into five groups: untreated group, cold water-treated group, cold water and WZXTR-treated group, cold water and dispersing phlegm and eliminating stagnation recipe (Xiaotan Sanjie Recipe, XTSJR, another compound traditional Chinese herbal medicine) treated group, cold water and tegafur-treated group. Mice in the latter 4 groups were fed 0-4 degrees centigrade cold distilled water 10 ml/(kg x d) one week before tumor inoculation for 3 weeks. Mice in the 5 groups were inoculated neoplastic cells which were diluted 2 x 10(7)/ml with normal saline in the right armpit at the second week. Forty-eight hours later, the mice were given drugs intragastrically (WZXTR, XTSJR and tegafur, respectively). The mice were continually intervened by cold water in the morning and given drugs in the afternoon. Mice in the cold water-treated group were given normal saline, and nothing was given in untreated group. Morphology appearance and changes of rectal temperature were observed, and the tumor weight and volume were measured and inhibitory rate was calculated 22 days after tumor inoculation. IL-8 was detected by avidin-biotin system-enzyme-labeled immunosorbent assay (ABC-ELISA) and real-time reverse polymerase chain reaction (real-time PCR). RESULTS: The content of IL-8 in tumor tissue of the untreated group was significantly higher than that of the other groups (P<0.01), and that of the cold water-treated group was higher than that of the cold water and WZXTR-treated group (P<0.01) and the cold water and XTSJR-treated group (P<0.05), respectively. The gray scale value of cDNA amplification strap was descent more evidently in both the cold water and WZXTR-treated group and the cold water and XTSJR-treated group as compared with that of the cold water-treated group and the untreated group (P<0.05). CONCLUSION: WZXTR can restrain tumor growth, and the mechanism may be that it can degrade the expression of IL-8 in S180 tumor.

Adolescent ; Adult ; Anxiety ; epidemiology ; China ; epidemiology ; Female ; Humans ; Interior Design and Furnishings ; Male ; Middle Aged ; Occupational Health ; statistics & numerical data ; Quality of Life ; Young Adult

Accidents, Occupational ; Humans ; Work Schedule Tolerance

Background Clinical researches showed that antimicrobial-resistance of bacterium is gradually serious.It is very important to master pathogen distribution and antimicrobial susceptibility after eye surgery.Objective This study was to investigate the distribution and drug sensitivity of pathogenic bacteria from ocular operative infection in 3-year duration to provide a reference for reasonable selection of antibiotics.Methods Sixtyfive positive specimens were obtained from 65 patients due to postoperative infection in Department of Ophthalmology of Shenzhou Hospital and Shengjing Hospital from 2009 January through 2011 December.The pathogenic bacteria were isolated and then the drug sensitivity test of conventional antibiotics was performed.Results Among the 65 ophthalmic specimens,35 strains of Gram-positive bacteria were isolated with the constituent ratio 53.8％,and 15 strains of Gram-negative bacteria accounted for 23.1％.Fungus was found in 15 cases with the constituent ratio 23.1％.Staphylococcus epidermidis accounted for 42.9％ in Gram-positive bacteria,and Pseudomonas aeruginosa accounted for 33.3％ in Gram-negative bacteria.Aspergillus was the main component of fungus,which accounted for 60.0％ of the proportion 3 strains of methicillin-resistant Staphylococcus aureus (MRSA) in 8 strains of Staphylococcus aureus,but no vancomycin-resistant Enterococcus (VRE) and vancomycin-resistant Staphylococcus (VRS) were seen.The in vitro drug sensitivity test showed that the main Gram-positive bacteria,Staphylococcus epidermidis and Staphylococcus aureus,were sensitive to linezolid,vancomycin,levofloxacin and ciprofloxacin with the sensitive rates ＞50.0％,and these bacteria were lowly sensitive to penicillin and erythromycin with the sensitive rates ≤30.0％.Pseudomonas aeruginosa appeared to be the main Gram-negative bacteria,showing a sensitive rate ≥ 80.0％ to tobramycin,amikacin,ceftazidime,cefepime,cefoperazone/sulbactam,piperacillin/tazobactam,imipenem and meropenem.Conclusions Staphylococcus epidermidis,Staphylococcus aureus,Pseudomonas aeruginosa and Aspergillus are primary pathogens in ocular infection after operation.Antibiotics such as penicillin,erythromycin,levofloxacin,cefotaxime seem to be relatively high resistant to eye infection.To choose sensitive antibiotics timely and reasonably is a key to the prevention and control of ocular infection after operation.

AIM: To compare the ligustilide content in Siwu Decoction and its ingredient herb. METHODS: To extract with SFE-CO 2 and to determine ligustilide with HPLC. RESULTS: Of the content of ligustilide, the highest in single herb, next in the mixture of Angelicae Sinensis Radix and Lignstic Rhizoma, the lowest in Siwu Decoction. CONCLUSION: When Angelicane Sinensis Radix and Lignstic Rhizoma were in compound preparation, the content of ligustilide became lower obviously.

OBJECTIVE:To provide scientific reference for rational,effective and economical drug use in pediatric depart-ment. METHODS:A total of 9771 prescriptions were randomly selected from children's branch of our hospital during May 2012-Apr. 2016. Those prescriptions were analyzed statistically in respects of drug type,prescription cost,utilization rate of injec-tion,utilization rate of essential medicine,clinical diagnosis,irrational drug use,use of antibiotics,etc. RESULTS:Among 9771 prescriptions,2.91 types of drugs were used in each prescription,and each prescription expended 77.10 yuan;utilization rate of in-jection was 57.11％,and that of national essential medicine was 67.80％. Respiratory tract disease was main disease (84.17％). There were 284 irrational prescriptions(2.91％),including 156 non-standard prescriptions,165 unsuitable prescriptions and 65 ex-traordinary prescriptions. Utilization rate of antibiotics was 18.25％,among which that of Cefoxitin sodium injection was the high-est(15.48％),but its utilization index was the lowest(0.58). The detection rate of microorganism isolated from bronchitis patients was in low level(45.10％). CONCLUSIONS:The rate of qualified prescription in the children's branch of our Hospital is higher than the requirement of the former Ministry of Public Health concerning the rate of qualified prescription >95％;utilization rate of antibiotics and injection are both in high level. In the future,it is necessary to strengthen prescription evaluation and promote stan-dard and rational use of drugs so as to guarantee safe,effective and economical use of drugs in clinic.

Objective To discuss the relationship between pulmonary infection, offline difficulties and airway abnormalities and to discuss the type of airway abnormalities. Methods The clinical data of 43 patients of severe pneumonia with inspection of electronic fibro-bronchoscope in PICU from Feb 2008 to May 2010 were retrospectively analyzed. Results In the 43 cases,24 cases existel different degrees of airway abnormalities, most of airway cartilage softening,in 15 cases; laryngeal dysplasia in 3 cases; absence of the right bronchus and right lung in 1 case. Conclusion Many children may exist airway abnormalities,if they have such performance as the clinical manifestations of recurrent wheezing after birth, refractory respiratory infection or difficulties in clinical offline, the increase of PCO2 though the effective suction, paroxysmal cyanosis and excluded with heart disease. Those children should undergo inspection with electronic fibro-bronchoscope to confirm the diagnosis as early as possible.