Objective To evaluate the role of PI3K-Akt-HIF-1α signaling pathway in dexmedetomidine-induced reduction of damage to the unventilated lung during one-lung ventilation (OLV) in rats.Methods Forty adult male Sprague-Dawley rats,weighing 250-350 g,were randomly divided into 4 groups (n=10 each) using a random number table:two-lung ventilation TLV) group,OLV + normal saline group (group OLV),OLV + dexmedetomidine group (group D),and OLV + dexmedetomidine +LY294002 group (group DL).Bilateral lungs were ventilated for 2.5 h in group TLV,and the right lung was ventilated for 2 h followed by 0.5 h TLV in the other groups.The rats were sacrificed,the left lung was removed for determination of wet to dry lung weight ratio (W/D ratio),and expression of phosphorylated Akt (p-Akt) and hypoxia-inducible factor-1 alpha (HIF-1α) (by Western blot).Results Compared with group TLV,W/D ratio was significantly increased,the expression of p-Akt was down-regulated,and the expression of HIF-1α was up-regulated in the other three groups (P＜0.05).Compared with group OLV,W/D ratio was significantly decreased,and the expression of p-Akt and HIF-lα was up-regulated in D and DL groups (P＜0.05).Compared with group DL,W/D ratio was significantly decreased,and the expression of p-Akt and HIF-1α was up-regulated in group D (P＜0.05).Conclusion PI3K-Akt-HIF-1α signaling pathway is involved in dexmedetomidine-induced reduction of damage to the unventilated lung during OLV in rats.

Asia ; epidemiology ; Humans ; Non-alcoholic Fatty Liver Disease ; epidemiology ; Prevalence

The deposition of amyloid β-protein (Aβ is the key pathological basis of cerebral amyloid angiopathy (CAA) and Alzheimer's disease (AD). The current studies emphasis that the stability of internal environment between cells of the neurovascular unit is the key to maintain normal brain function. This article reviews the relationship between the Aβand the neurovascular unit injury, and we expect to provide some new ideas for the clinical prevention and treatment of CAA and AD.

DICOM standard is a compulsory international standard for the establishment of Integrated Hospital Information System (IHIS) and Picture Archiving and Communication System (PACS). This paper introduces such information of DICOM3.0 as its generation, components, operational mechanism and application. To understand DICOM standard exactly is helpful for the efficient operation of PACS.

There are many therapeutic drugs for rheumatoid arthritis(RA) treatment,but the pathogenesis of RA have not been fully understood,therefore no curable drug has been found.With increasing knowledge of the inflammatory process,especially in signaling,some drugs modifying disease progress and interfering signaling pathways have been studied.Cytokine inhibitors such as tumor necrosis factor-blocking agents and IL-1 receptor antagonist in clinical trials and in practice have resulted in dramatic improvement in RA disease control.Important molecular mechanisms of actions on total glucosides of paeony(TGP) and Glucosides of Chaenomeles speciosa(GCS) were showed to modulate G protein-AC-cAMP and Ras-MAPKs signaling pathways and to inhibit synoviacytes proliferation.This review focuses on agents which target various cytokines,other inflammatory mediators,antigen of cells,and signaling pathways.

Objective:To perform in vitra MR imaging and compare the differences between DepoGd and Gd-DTPA in signal intensity using multivesicular liposome(DepoFoam)as the carrier of rutine magnetic resornance contrast media.Methods:Lipos ome such as cholesterol was mixed with Gd-DTPA,and rotation speed of centrifuge was adjusted to get DepoGd particles with certain diameter.The three different liquors named X,Y and Z(Gd-DTPA liquor,17 ?m DepoGd liquor and 22 ?m DepoGd liquor) were prepared into 4 kinds of concentrations spectively.Signal intensity of these liquors was measured with MR scanner,and statistical analysis was done consequently.Results:Diameters of most of the DepoGd particles got through the forementioned method with the envelop rate of 50%.Signal intensity of different liquors with the same concentration showed no statistical difference,which means signal intensity of DepoGd and Gd-DTPA active compound with the same mole concentration showed no statistical difference in vitra MR test.Conclusion:DepoFoam can carry conventional MR contrast agent with high envelop rate and no obvious difference was showed in signal intensity.

Objective To discuss the medication rules of Glycyrrhizae Radix et Rhizoma in Shang Han Lun based on dose-response relationship;To provide references for clinical application. Methods It systematically arranged the provisions containing Glycyrrhizae Radix et Rhizoma in Shang Han Lun. It statistically analyzed the relationship among Glycyrrhizae Radix et Rhizoma and other various factors by using SPSS 20.0. Results After screening, 70 prescriptions were included, among which 2 (2.86%) contained raw Glycyrrhizae Radix et Rhizoma and 68 (97.14%) contained Glycyrrhizae Radix et Rhizoma Praeparata cum Melia. The taken dose of every time had a close relationship with the number of medicine, water consumption, residual water, water consumption per time and frequency of use, which had a statistical significance (P<0.01). Univariate logistic regression analysis showed whether Glycyrrhizae Radix et Rhizoma as a monarch medicine had the close relationship with the number of medicine (P=0.029). In 48(73.85%) prescriptions contained Glycyrrhizae Radix et Rhizoma Praeparata cum Melia as non-monarch medicine, the taken dose of every time and the number of drugs had statistical differences with that in the prescriptions which as monarch medicine (P=0.000). When Glycyrrhizae Radix et Rhizoma Praeparata cum Melia was in compatibility with other medicine, its dose had a certain rules with the number of medicine. When it was the basic medicine, there was no compatibility with Zingiberis Rhizoma Recens, Jujubae Fructus, and Ginseng Radix et Rhizoma. Conclusion It can be found from the dose-response relationship that Glycyrrhizae Radix et Rhizoma has a widely dose and combination efficacy in different prescriptions. Its flexibility, diversity and scientific nature provide the solid basis for clinical application.

OBJECTIVE:To review the current situation and the trend of development of oral hypoglycemic agents in our hospital.METHODS:The defined daily dose DDDs and the sales amount of oral hypoglycemic agents used in our hospital during2001～July,2004were analyzed statistically.RESULTS:The DDDs of sulfonylureas was decreasing,whereas it still accounted for more than70%of the total DDDs;the glipizide ranked the first in DDDs during these years;the DDDs of biguanides kept increasing year by year;the sales amount of?-glucosidase inhibitor and thiazolidine diketone drugs was in?creasing;all the‘Medical Insurance’drugs accounted for more than97%of the total DDDs.CONCLUSION:The oral hy?poglycemic agents used in our hospital is rational on the whole.

Mass spectrometry is an important and powerful tool for protein quantification. With the in-depth development of quantitative proteomics, limitations of classic MS based quantification methods, such as complicated matrix interference and throughput/capacity limitation, start to appear. Recent progress of series novel MS based techniques provide effective solutions for the limitations of relative and absolute proteomic quantification, including synchronous precursor selection ( SPS ) , mass defect isobaric labeling, parallel reaction monitoring ( PRM) , multiplexing acquisition ( MSX) , and various novel data independent acquisition ( DIA) modes. Here we summarized the current limitations of quantitative proteomics, reviewed the latest MS based quantification approaches, and discussed the features and advantages of these novel techniques for quantitative proteomic application.

Objective To study the physiochemical properties of selenium exopolysaccharide from Porphyridium sp.(Se-PSP).Methods The Se-PSP was obtained from ASW,a sort of culture medium added with some selenious acid solution,and was purified by DEAE-52 cellulose column and identified by Sephadex G-200.The acidity,UV spectral absorption,paper chromatography,and gelose electrophoresis of Se-PSP solution were studied.Results and Conclusion Proper concentration of selenious acid in ASW culture had no influences upon the growth of Porphyridium.Se-PSP prepared in this way didn't contain protein and nucleic acid and the solution of Se-PSP showed acidity.It is probable that Se-PSP contain two fractions of PSP.