Objective:To research the effects of HH singaling pathway on proliferation,apoptosis and CyclinD1 expression of breast cancer MDA-MB-231 cells by MDA-MB-231 cells treated with Cyclopamine,a HH singaling pathway inhibitor.Methods:MDA-MB-231 cells were treated with Cyclopamine.Cell proliferation was detected by MTT;Cell cycle distribution and apoptosis were analysed by flow cytometry(FCM).The mRNA levels of GLI1,CyclinD1 were measured by reverse transcription-polymerase chain reaction(RT-PCR);The protein level of CyclinD1 was detected by immunostaining of cell lines.Results:Compared with control group,Cyclopamine could significantly inhibit the proliferation of MDA-MB-231 cells in a dose-and time-dependent manner;By FCM the treatment of MDA-MB-231 cells with Cyclopamine induced a remarkable increase in the proportion of cells in the G0/G1 phase of the cell cycle and after 48 h the sub-G1 peak appeared.The mRNA levels of GLI1,CyclinD1 and the expression of CyclinD1 protein were reduced.Conclusion:The HH signaling pathway was activeted in MDA-MB-231 cells.HH signaling pathway inhibitor,Cyclopamine,could inhibit the proliferation,induce the apoptosis and reduce the expression of CyclinD1 in MDA-MB-231 cells.

Objective To evaluate the clinical effects of recombinant human epidermal growth factor (rhEGF) on wound healing of perianal absceess after operation.Methods 80 patients who received operation of perianal absceess were selected.The patients were randomly divided into the study group(n=40) and control group(n=40).All the patients underwent the treatment of anal abscess incision and drainage.The patients in the control group were disinfected routinely and treated by metronidazole,while the patients in the study group were treated by rhEGF after disinfection of saline.All patients were followed up for one month after surgery.The wound edema score,granulation growth and the healing time of the wound were recorded.Results One month after surgery,the wound edema score in the study group was less than that in the control group at any time.After 2 weeks and 4 weeks,the wound edema scores in the study group were (1.41±0.33)points,(0.54±0.11)points,which were significantly lower than (2.09±0.28)points,(1.37±0.16)points in the control group (t=9.937,27.036,all P<0.001).After 2 weeks and 4 weeks,the granulation growth scores in the study group were (1.71±0.38)points,(0.81±0.16)points,which were significantly lower than(2.16±0.38)points,(1.66±0.21)points in the control group (t=5.296,20.363,P<0.001).The healing time of the wound in the study group was (19.52±1.47)d,which was less than (25.49±1.75)d in the control group (t=16.521,P<0.05).Conclusion The rhEGF can distinctly promote wound healing and shorten the healing time of perianal absceess after operation,which is worthy of the clinical promotion.

Objective:Investigation on the characteristics of motorcycle accidentrelated fatalities,to provide the reliable information and reference data for the precaution and the reduction of the death rate of the traffic accident.Methods:Collect the information of the 145 people dead in the 135 cases of motorcycle accident which happened in the main urban zone of Chongqing city in 2004 examinated by the Chongqing Technical Authentication Center Of Road Traffic Accident,including the detailed process,time and place of accident as well as the means of transportation,the basic information,the injuries,and the main cause of death,which all were retrospectively analyzed.Results:Sex and age of the dead have the significant difference.In the aspect of the means of transportation,59.31% of the dead are drivers who under 40 ocuppy 79%,20% are passengers,20.69% are pedestrians who over 50 ocuppy 60%,instantly dead occupy 36.55% and dead after salvage occupy 63.45%.In the aspect of the cause of dying,craniocerebral injury occupy 65.52%,other injury or combined injury occupy 34.48% and 95.17% of the dead with the limbs injury which the ratio of the fracture of lower limb and the fracture of arm is 2.1:1.And the ratio of the second,third season and the first,fourth season is 1.64:1.Conclusion:The motorcycle accidents have the obvious features and the preventability.

BACKGROUND:Tamoxifen has been found to exert neuroprotection by reducing cerebral hemorrhage and edema surrounding the injured site of the spinal cord.
OBJECTIVE:To investigate the neuroprotective effect of tamoxifen on rat acute spinal cord injury and the underlying mechanism.
METHODS:Sixty Sprague-Dawley rats were equivalently randomized into five groups, and modeled into spinal cord injury at T10 level using modified Al en’s weight-drop method (70 g/cm), except those in sham operation group. At 30 minutes after modeling, al rats were given the intraperitoneal injection of 2.5, 5.0 and 10 mg/kg tamoxifen or same amount of normal saline, once daily. Basso, Beattie, Bresnahan (BBB) scores were recorded at 24, 48 and 72 hours after surgery. The injured spinal cord was removed at 72 hours to observe its edema. Meanwhile, the levels of interleukin-1β, interleukin-10 and tumor necrosis factor-α, as wel as Caspase-3 activity were detected by ELISA;the protein levels of nuclear factor-κB p65, phosphorylated I-κBαand Caspase-3 were detected by western blot assay.
RESULTS AND CONCLUSION:Compared with the model group, the hind limb function in the tamoxifen groups was significantly improved. Tamoxifen significantly decreased the water content in the rat spinal cord and inhibited spinal cord edema at 72 hours after surgery. ELISA results showed that tamoxifen significantly reduced the activity of interleukin-1β, interleukin-10, tumor necrosis factor-αand Caspase-3 (P<0.05). Western blot assay revealed that tamoxifen significantly downregulated the expression levels of nuclear factor-κB p65, phosphorylated I-κBαand Caspase-3. These results suggest that tamoxifen protects against spinal cord injury via suppressing inflammatory response and apoptosis-associated proteins.

Objective To investigate the histological detection and pleural effusion Mycobacterium tuberculosis and adenosine deaminase(ADA) activity assay value of tuberculous pleurisy.Methods 30 patients with tuberculous pleurisy were chosen as the research object,and selected another non-tuberculous pleurisy caused by pleural effusion in 30 patients as controls,patients received pleural puncture,take detect pleural effusion and pleural biopsy.Results A biopsy to confirm the diagnosis of tuberculous pleurisy in 18 cases,compared to 60.0 percent.Six cases of patients with pleural tissue biopsy again,a ratio of 20.0％.Pleural histopathology diagnosis was 80.0％.Patients with tuberculous pleurisy pleural effusion ADA average activity was (71.3 ± 16.2),which was significantly higher than that of the control [(19.3 ± 10.8)] (t =23.42,P ＜ 0.05).ADA-positive patients with tuberculous pleurisy was 86.7％,which was significantly higher than 3.3％ of the confrol group (x2 =14.64,P ＜ 0.05).Patients with tuberculous pleurisy pleural effusion TB-PCR positive rate was 6 6.7 ％,significantly higher than the 2 0.0 ％ (x2 =8.6 5,P ＜0.05).Application and histopathological examination of pleural effusion TB-PCR and ADA joint detection,diagnosis of tuberculous pleurisy was 100.0％,significantly higher than that of various methods alone(x2 =4.36,5.87,4.16,all P ＜ 0.05).Conclusion Histopathological examination of pleural effusion TB-PCR and ADA joint detection can improve diagnostic accuracy of tuberculous pleurisy.

Chondroblastoma of the patella, rare occurred in patellar,is a kind of an uncommon benign bone tumor. Compared with giant cell tumor, the morbidity of chondroblastoma is lower. Meanwhile, its clinical manifestations are various, and images are very complicated. Therefore, the understanding of this kind of tumors may be limited even to the orthopedist. The differences of patellar chondroblastoma between other tumor in X-ray, CT and MRI is a spot in recent years. Sometimes patellar chondroblastoma coexists with aneurysmal bone cyst, which is a challenge to obtain an accurate pathological and radiological diagnosis. For the treatment, curettage and bone grafting is one the most popular method, but whether to perform a biopsy before surgery still remain controversy. Some new technique still has an unknown prospect for the treatment such as radiofrequency ablation.
Bone Neoplasms ; diagnosis ; surgery ; Chondroblastoma ; diagnosis ; surgery ; Humans ; Patella ; surgery

Objective To determine the protein levels of receptor activator of NF ?B ligand (RANKL) in synovial tissues obtained from ankylosing spondylitis (AS) patients, and compare the expression level and distribution of RANKL protein in AS with that in rheumatoid arthritis (RA), osteoarthritis (OA) and normal synovial tissues, in order to define the role of RANKL expressions in the pathogenesis of arthritis and bone destruction in AS. Methods Immunohistochemical analysis was performed using monoclonal antibodies to determine RANKL expression in 13 AS, 16 RA, 17 OA patients and 6 healthy controls. The protein levels of RANKL in labeled synovial tissue sections were quantified by digital image analysis and semiquantitative analysis to compare the expression of RANKL in positve cells among different patient groups and normal subjects. In addition, RANKL expression was correlated with certain inflammatory indices (including ESR, CRP, blood platelet count) and radiological stage of involved joints, respectively. Results Positive staining of RANKL was seen respectively in all 13 AS patients and 16 patients with RA, and the positive expression was distributed predominantly in the synovial lining layer and at synovium cartilage junctions. There was no significant difference between levels of RANKL expression in tissues from patients with AS and in tissues from RA. No positive staining of RANKL was observed in 6 normal subjects and all OA patients. Positive correlation was found between RANKL protein expression and X ray stage of bone destruction of involved joints in the patients with AS and RA ( r =0 73,0 41, P =0 003,0 021 respectively). Conclusions RANKL plays an important role in the pathogenesis of bone destruction in patients with AS, and its elevated expression level may reflect the degree of bone destruction of peripheral joints in AS patients. Since similar patterns of RANKL expression are found in synovial tissues from AS and RA patients, therefore, we conclude that the pathogenesis of bone destruction in AS may be similar to that in RA

Gene expression profile technology is characterized by high automation, high accuracy and large information volume, which provides a new way for the development of individualized therapies and novel therapeutic targets. Some researches have identified sets of discriminating genes by using gene expression profile technology that can be used for the predietion of response to radiotherapy in human cancers. Some studies also have confirmed the therapeutic tagerts of radiation sensitizer.

Objective To study the characteristics of serum lipid metabolism in cadres aged 90 and above.Methods A total of 105 in-patient cadres aged 90 and above were assigned to the longevity group,and another 85 in-patient cadres aged 60 to 80 years were used as controls.Medical history of the participants were retrospectively reviewed,and serum levels of total cholesterol(TC),triglyceride(TG),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),apoB,apoA-I,Lp(a)and body mass index(BMI)were tested.Results Compared with the control group,TC,TG,LDL-C,apoB and non-HDL-C were significantly decreased in the longevity group[3.90 +0.89 vs 4.36 ± 1.06,0.89(0.57)vs 1.16(0.69),2.35 ±0.72 vs 2.70 ±0.88,0.68 ±0.19 vs 0.75 ±0.22,2.69 ±0.79 vs 3.18 ± 1.00,respectively].However,HDL-C/TC and HDL-C/LDL-C were significantly increased in the longevity group [0.32 ±0.084 vs 0.28 ±0.082,0.52(0.22)vs 0.43(0.28),respectively].Lp(a)of the longevity group was higher than that of the control group,although no statistically significant difference was found.BMI of the longevity group was significantly lower than that of the control group.In the longevity group,the percentage of individual who were combined with hypertension,hyperglycemia,hyperlipidemia and overweight/fat was lower (24.8％ vs 43.5％).Conclusions Serum lipid level is relatively lower in cadres aged 90 and above,and those who are combined with hypertension,hyperglycemia,hyperlipidemia and overweight/fat are significantly lower in this populations.

Objective:To evaluate the surface free energy and Streptococcus mutans(S.mutans)adhesion on the surfaces of Co-Cr al-loy specimens fabricated by selective laser sintering(SLS)and casting method(SG and W280)respectively.Methods:The specimens of three groups were polished by SiC sandpaper.Surface morphology test,surface contact angle test and surface adhesion test of S.mu-tans were performed.Results:With the same surface roughness value,the surface contact angle of group SLS,W280 and SG was 66.200 ±3.112,74.212 ±3.206 and 67.238 ±2.047(P >0.05),the surface adhesion of S.mutans colone was 8.88 ±2.90,9.25 ± 2.05 and 8.50 ±1.85(P >0.05),respectively.Conclusion:The cobalt chromium alloy fabricated by SLS and casting method have similar surface performance.