Objective:To develop a new nucleic amplication method for detection of Chlamydia trachomatis DNA by gap ligase chain reaction(G-LCR).Methods:A G-LCR DNA amplification assay that targeted the outer major membrane protein gene(omp1)of CT was established to detect CT infection.The sensitivity and specificity of a newly developed G-LCR test was examined by the use of highly purified elementary bodies (EBs).DNA fragments of different species and from other bacteria1 were detected with G-LCR and routine polymerase chain reaction (PCR).Results:Using G-LCR,DNA fragments of 54bp were amplified from five different species.The sensitivity could be improved to detect out 2 chlamydial elementary bodies.G-LCR detected ten-fold EBs than PCR.No signal was observed when C.pneumoniae and other bacteria were used as templates.Conclusion:G-LCR is sensitive,rapid and specific for detection of Chlamydia trachomatis.

It is generally agreed that motivation is one of the most important factors influencing the students success or failure in learning a language. Motivation, according to Harmer, is an internal drive that encourages the learner to pursue a course of action(1983: 3). Expressed more colloquially, when students perceive an attractive goal that they wish to achieve, they may do whatever is necessary to reach it. Then they are strongly motivated.This article aims at introducing the classification teaching method into English teaching. Studies suggest that classification teaching method has positive effects on motivation.

In view of the fact that the Pharmaceutical Patent Law will be put into effect in China,the authors suggest that the development of new drugs in China should be relied mainly rather on creating than on copying. However,some of the effective drugs should be still modelled on foreign products. We should pay great attention to the research work on pharmaceutical preparations and the development of Chinese patent medicine, intermediates of preparation and reagents.

Fatal Outcome ; Humans ; Infant, Newborn ; Kidney ; abnormalities ; Male ; Pneumothorax ; etiology ; Respiratory Distress Syndrome, Newborn ; etiology ; Syndrome

Objective:To explore the relations between freshmen's self-identity status,personality characteristics and mental health status.Methods:188 new students were asked to complete the questionnaires of mental health scale and personality scale from the China college students mental health evaluation system,and eollnge students self- identity status scale.Results:①the most status of identity is achievement(43.1%),then the identity diffusion (30.9%);②male students in compare with female,were more likely in achievement status(60.7?13.8/53.9?11.4,P0.406);③the achieve- ment status had obvious negative correlation with depression,dependency(r=-0.17～-0.24),the diffusion status had obvious positive correlation with depression,social-withdrawal,and impulsion(r=0.15～0.34);④active,ac- commodating,tough-minded personality characteristics had obviously negative correlation with mental symptoms(r= -0.16～-0.56).Conclusions:encourage college students to form the achievement ego-identity status,to be ac- tive,accommodating and tough-minded,will be helpful for their psychological health.

Objective To investigate the positive and suspicious rates of BV, SRV, SIV and STLV-1 antibody of the cynomolgus monkeys introduced from Laos, dynamic monitoring of virus antibody during the establishment of SPF cyno-molgus monkey population, and then comparing the positive rate of virus antibody in the baby monkeys between the common with SPF populations.Methods Continuous monitoring four kinds of virus in the monkeys was carried out using special reagent kits and analyzed the data.Results In the introduced cynomolgus monkeys (n=1998), the positive rate of BV antibody was 52.35%, suspicious rate of BV antibody was 8.31%, and negative rate of BV antibody was only 39.34%, the positive rates of SRV and STLV-1 antibody were 7.45%and 8.56%, respectively, and positive or suspicious SIV anti-body in the cynomolgus monkeys was not detected.After screening and establishment of the SPF population, the positive rates of BV, SRV and STLV-1 antibody were 5.24%, 1.01%and 0.4%, respectively, in the year 2010.Then after five years of screening and elimination, by the end of 2014, the positive rates of BV, SRV and STLV-1 antibodies were 0.82%, 0.27%and 0.27%, respectively, and positive or suspicious SIV antibody in the cynomolgus monkeys was not de-tected.The BV antibody positive rate of the baby monkeys was 9.71%and the suspicious rate was 1.85%in the common population, but only 0.22%of the baby monkeys in the SPF population.Conclusions Continuous monitoring virus anti-bodies and eliminating positive and suspicious animals have important significance in the establishment of a SPF cynomolgus monkey population.

Objective To investigate the effects of different oxygen inhalation modes on retinal vessels development in neonatal rat in order to approach its etiopathogenesis and provide experimental evidence for proper oxygen therapy for premature.Methods Totally 150 postnatal day 7(P7)Wistar rats were randomly assigned into 5 groups according to different oxygen inhalation modes(n=30 in each group).Experimental group 1 were exposed to 75%,60%,50%,40% and 30% oxygen in turn for one day,followed by room air exposure for 5 d.Experimental group 2 were exposed to 30%,40%,50%,60% and 75% oxygen in turn for one day,then exposed in air for 5 d.Experimental group 3 were exposed to 75% oxygen for 5 d,then oxygen supply was suddenly stopped,followed by room air exposure for 5 d.Experimental group 4 were exposed to 75% oxygen for 5 d,then the oxygen supply was reduced by 10% per day till oxygen supply level equal to room air,finally room air exposure for 5 d.The control group were exposed to room air in the same experimental condition.The retinal vascular development and proliferation were evaluated by stretched preparation(ADPase stained retina)and eyeball cross-section.The nuclei of proliferative retinal vessels of 5 groups were counted and the vascular endothelial growth factor(VEGF)expressions in retina were detected by immunohistochemical staining.The dependability between the nuclei of proliferative retinal vessels and the VEGF expressions in retina were also analyzed.Results In the experimental groups 2 and 3,there existed retinal hypoxia and formed abnormal new vessels as retinopathy of prematurity(ROP).The VEGF expressions increased after hypoxia,mainly in endochylema and nucleus of capillary endothelium of ganglionic layer of retina and inner nuclear layer.Linear correlation analysis of the data indicated the positive correlation was between the nuclei of the proliferative vessels and the VEGF levels in retina(r=0.807,P

Objective: To investigate the rate of depression in patients with functional dyspepsia (FD) and corresponding treatments Method:285 out-patients diagnosed as FD were assessed by two physician trained for depression assessment Depression was diagnosed by criteria of CCMD-2-R Patients with depression were divided into three groups: group A (n=28) received venlafaxine, group B (n=31) received doxepine, group C (n=26) received no antidepressant as control SDS (self-rate depression scale) and FD score were used to assess the effect of antidepressants Result:The rate of depression in our sample was 32% (92/285) Among 92 with depression, 85 consent to participate the study FD scores of both group A and group B decreased after 2 weeks treatment, and were significantly lower than that of group C at the end of 8 weeks The score of SDS in group decreased from the end of second week after treatment, and both group A and group B had significantly lower scores in SDS than group C at the end of 8 weeks Group A had fewer side effects than group B Conclusion:Depression is common in patients with FD Antidepressant can improve FD symptoms as well as depressive symptoms Venlafaxine has fewer side effects than doxepine

Objective To study the genetic polymorphism of Nanjiang yellow goat and to investigate the feasibility of this research by AFLP. Methods The genomic DNAs of 15 goats were analyzed with RAPD and AFLP molecule marking techniques. The effects of the two methods were compared. Results The rates of the polymorphic loci and the genetic similar index of population by RAPD marker were 26.98% and 0.942 respectively, and that by 31.4% AFLP and 0.874 respectively. Conclusion AFLP and RAPD technique are effective methods in genetic polymorphism monitoring of Nanjiang yellow goats, and supply useful parameter with appraise genetic stability.

Objective To investigate the pathological changes of ultrastructure of mucosa in various bronchial segments from type 2 diabetic patients. Methods Sixteen cases of type 2 diabetic patients were selected,2-3 pieces of bronchial mucosa and submucosal tissue of the lesion were taken from various bronchi during bronchoscopy and these samples were observed under a transmission electron microscope. Results The basal lamina of bronchial capillary were diffusely thickened and mostly showed onion-skin like change,protein deposited around and mixed with basal menbrance;irregular highly electron dense materials were found to deposite around capillary,capillary lumen became narrow or even collapsed,neutrophilic leucocyte marginated in lumen and adhered with endothelium;protein deposited in the interstitial;endothelial cells and pericytes had dark cell changes.The cistern of rough endoplasmic reticulum dilated and vesicle formed.Conclusion Bronchial mucosa and its surrounding tissues show characteristic pathological changes of diabetes,bronchial is also the target organ of chronic diabetic damage.