1.Polymer formation and altered biodistribution of IgG labelled with Tc and cyclic DTPA.
Sang Moo LIM ; Kwang Sun WOO ; Wee Sup CHUNG ; Ok Doo AWH
Korean Journal of Nuclear Medicine 1993;27(2):270-276
No abstract available.
Immunoglobulin G*
;
Pentetic Acid*
;
Polymers*
2.Distribution of 123I, 99mTc-human polyclonal nonspecific IgG and 67Ga-citrate in abscess bearing mice.
Sang Moo LIM ; Kwang Sun WOO ; Wee Sup CHUNG ; Ok Doo AWH ; Yong Sup SEO ; Jong Doo LEE
Korean Journal of Nuclear Medicine 1992;26(1):116-123
No abstract available.
Abscess*
;
Animals
;
Immunoglobulin G*
;
Mice*
3.In Vivo Image and Biodistribution of 123I-15-(p-iodophenyl)-3-R , S - methylpentadecanoic acid ( BMIPP ) in Liposarcoma Bearing Nude Mice.
Chang Woon CHOI ; Sang Moo LIM ; Tae Sup LEE ; Yong Sup SUH ; Kwang Sun WOO ; Wee Sup CHUNG ; Soo Jung LIM ; Ok Doo AWH
Korean Journal of Nuclear Medicine 2001;35(5):324-333
No abstract available.
Animals
;
Liposarcoma*
;
Mice
;
Mice, Nude*
4.Treatment of an 8-mm Myxoma Using Acellular Corneal Tissue.
Kyung Sup LIM ; Sung Wook WEE ; Jae Chan KIM
Korean Journal of Ophthalmology 2014;28(1):86-90
A myxoma is a benign tumor found in the heart and in various soft tissues; however, a corneal myxoma is rare. A mucinous mass of unknown etiology was observed on the left cornea of a 32-year-old male patient. We performed deep anterior lamellar keratoplasty using acellular corneal tissue and concurrent amniotic membrane transplantation. Hematoxylin and eosin staining revealed vacuolation of the parenchyma and myxoid change in the corneal tissue that occurred in the anterior half of the corneal parenchyma. We identified a myxoid stroma by Alcian blue staining and observed collagen fibers with denatured stroma by Masson trichrome staining. The patient's visual acuity improved from light perception to 20 / 200, and the intraocular pressure remained within the normal range for one year after surgery. The transplanted cornea survived successfully with well-maintained transparency, and recurrence was not observed one year after surgery.
Adult
;
*Cornea
;
Corneal Stroma/cytology/*transplantation
;
Corneal Transplantation/*methods
;
Eye Neoplasms/diagnosis/*surgery
;
Humans
;
Male
;
Myxoma/diagnosis/*surgery
5.Treatment of an 8-mm Myxoma Using Acellular Corneal Tissue.
Kyung Sup LIM ; Sung Wook WEE ; Jae Chan KIM
Korean Journal of Ophthalmology 2014;28(1):86-90
A myxoma is a benign tumor found in the heart and in various soft tissues; however, a corneal myxoma is rare. A mucinous mass of unknown etiology was observed on the left cornea of a 32-year-old male patient. We performed deep anterior lamellar keratoplasty using acellular corneal tissue and concurrent amniotic membrane transplantation. Hematoxylin and eosin staining revealed vacuolation of the parenchyma and myxoid change in the corneal tissue that occurred in the anterior half of the corneal parenchyma. We identified a myxoid stroma by Alcian blue staining and observed collagen fibers with denatured stroma by Masson trichrome staining. The patient's visual acuity improved from light perception to 20 / 200, and the intraocular pressure remained within the normal range for one year after surgery. The transplanted cornea survived successfully with well-maintained transparency, and recurrence was not observed one year after surgery.
Adult
;
*Cornea
;
Corneal Stroma/cytology/*transplantation
;
Corneal Transplantation/*methods
;
Eye Neoplasms/diagnosis/*surgery
;
Humans
;
Male
;
Myxoma/diagnosis/*surgery
6.Comparison of Direct-labeling Method of Antibody with 99mTc and 188Re.
Chang Woon CHOI ; Sang Moo LIM ; Kwang Sun WOO ; Wee Sup CHUNG ; Tae Hyun CHOI ; Soo Jeong LIM
Korean Journal of Nuclear Medicine 1999;33(1):84-93
PURPOSE: We investigated the direct labeling method of antibody with 99mTc and 188Re and examined the stability and function of these labeled compounds in in vitro and in vivo. MATERIALS AND METHODS: Disulfide bond of nonspecific human IgG was reduced to -SH group by 2-mercaptoethanol. Stannous ion was used to reduce 99mTc and 188Re. The stability of 99mTc-IgG and 188Re-IgG was estimated upto 24 hrs. Biodistribution was evaluated in abscess bearing rats at 4 and 24 hr post-injection of 99mTc or 188Re labeled IgG. RESULTS: The number of -SH group per reduced IgG molecule was 2.34. The labeling yield of 99mTc-IgG and 188Re-IgG were 90% and 95%, respectively. The stability of 99mTc-IgG at 1, 4, 6 and 24 hr was 91%, 83%, 78%, 7% and that of 188Re-IgG, high uptake was found on kidney, blood, stomach and abscess (9.42+/-0.68, 1.43+/-0.24, 0.86+/-0.18, 0.72+/-0.10 %ID/g, respectively). The uptakes at 24 hr were kidney, abscess, stomach, and blood in descending order. In case of 188Re-IgG, high uptake at 4 hr post injection appeared on kidney, blood, abscess and stomach (3.92+/-0.62, 1.32+/-0.08, 0.88+/-0.01, 0.26+/-0.06, respectively). The upatkes at 24 hr were kidney, abscess, blood abd stomach in descending order. The abscess to blood uptake ratio of 99mTc-IgG was 0.5 at 4 hr and 2.02 at 24 hr and that of 188Re-IgG was 0.67 and 1.29. CONCLUSION: 99mTc-IgG and 188Re-IgG and 188Re-IgG canbe labeled efficiently with direct labeling method. However, 99mTc-IgG and 188Re-IgG, labeled with direct method, was unstable. Further study in needed to enhance the stability of the antibody labeling.
Abscess
;
Animals
;
Humans
;
Immunoglobulin G
;
Kidney
;
Mercaptoethanol
;
Rats
;
Stomach
7.Studies on Scintillation Proximity Assay for the mesurement of alpha - hCG.
Tae Hyun CHOI ; Chang Woon CHOI ; Sang Moo LIM ; Wee Sup CHUNG ; Soo Jeong LIM ; Su Jin LEE ; Tae Sup LEE ; Ok Doo AWH ; Kwang Sun WOO
Korean Journal of Nuclear Medicine 2002;36(2):133-139
No abstract available.
8.Neurofibromatosis Associated with Congenital Unilateral Pulsating Exophthalmos.
Wee Hyun NAM ; Poong Ho SONG ; Sang Sup CHUNG ; Chul Woo LEE
Journal of Korean Neurosurgical Society 1972;1(1):196-200
No abstract available.
Exophthalmos*
;
Neurofibromatoses*
9.Radioimmunoassay for Determination of Serum Macrophage Migration Inhibitory Factor.
Tae Sup LEE ; Seok Hwan SHIN ; Jee In SONG ; Kwang Sun WOO ; Wee Sup CHUNG ; Chang Woon CHOI ; Sang Moo LIM
Korean Journal of Nuclear Medicine 2004;38(6):532-539
PURPOSE: There has been a renewal of interest in Macrophage migration inhibitory factor (MIF), especially correlation in pathogenesis of sepsis by many infectious diseases and in regulation of host inflammatory and immune response. We developed immunoradiometric assay (IRMA) to determine serum human MIF concentration. MATERIALS AND METHODS: The IRMA system utilizes solid phase bound monoclonal anti-recombinant human MIF (rhMIF) antibody as a capture antibody, biotinylated polyclonal anti-rhMIF antibody as a detector antibody. We applied with rhMIF that concentration of standard solutions increased from 0 ng/ml to 100 ng/ml. We used 125I-streptavidin (SA) as radiotracer to determination of rhMIF concentration. Streptavidin was labeled with 125I by Chloramine-T method and 125I-SA was purified by ultracentrifugation. 125I-SA stability was evaluated by ITLC analysis at 4 degrees C and room temperatures until 60days. To validate IRMA system for MIF, we experimented intra-assay and inter-assay coefficients of variation, recovery test and dilution test. RESULTS: Radiolabeling yield of 125I-SA was 87% and purified 125I-SA retained above 99% radiochemical purity. 125I-SA showed above 93% stability in 4 degrees C until 60days that it is good for immunoradiometric assay as radiotracer. Plotted standard dose response curve showed that increased concentration of rhMIF linearly correlated (R2=0.99) with bound radioactivity of 125I-SA. The highest intra- and inter-assay coefficients of variation were 5.5% and 7.6%, respectively. The average of recovery of MIF in samples was 102%. In dilution test, linear response curves were obtained (R2=0.97). CONCLUSION: Radioimmunoassay using 125I-SA as radiotracer thought to be useful for the determination of serum MIF concentration, and further, its data will be used to evaluate the correlation between clinical significance and serum MIF concentration in patients with various inflammatory diseases.
Communicable Diseases
;
Humans
;
Immunoradiometric Assay
;
Macrophages*
;
Radioactivity
;
Radioimmunoassay*
;
Sepsis
;
Streptavidin
;
Ultracentrifugation
10.Biodistribution of 3-<131I>iodo-O-mythyl-L-alpha-methyltyrosine in Tumor Bearing Rats: A Comparison Study with L-3-<131I>iodo-alpha-methyltyrosine.
Chang Woon CHOI ; Seung Dae YANG ; Kwang Sun WOO ; Wee Sup CHUNG ; Soo Jung LIM ; Yong Sup SUH ; Kwon Soo CHUN ; Soon Hyuk AHN ; Jong Doo LEE ; Sung Soon HONG ; Sang Moo LIM
Korean Journal of Nuclear Medicine 1998;32(3):290-297
PURPOSE: The aim of this sutdy was to evaluate the feasibility of 3-[131I]Iodo-O-methyl-L-a-methyltyrosine ([131I]OMINT) as an agent for tumor image. MATERIALS AND METHODS: After synthesis of 4-O-methyl-L-a-methyltyrosine (OMAMT), OMAMT was labeled with 131I using Iodogen method. In viro cellular uptake study was performed using 9 L gliosarcoma cells at various time points upto 4 hr. The biodistribution (five rats implanted with the 9 L gliosarcoma cells per group) was evaluated at 30 min, 2 hr, 24 hr after iv injection of 3.7 MBq [131I]OMIMT or L-3-[131I]iodo-a-methyltyrosine ([131I]IMT). Gamma camera images were obtained at 30min, 2 hr, and 24 hr. RESULTS: [131I]OMINT uptake was 3.3 times and 2.5 times higher than [131I]IMT uptake at 30 min and 60 min, respectively and same after 2 hr in in vitro sutdy using 9L gliosarcoma cells. Maximum accumulation in tumor occurred at 30 min for both [131IOMINT and [131I]IMT in tumor bearing rats. The tumor uptake of [131I]OMINT was significantly higher than that of [131I]IMT in tumor bearing rats. The tumor uptake of [131I]OMIMT was significantly higher than that of [131I]IMT at early time point studied (3.74 +/- 0.48 vs 0.38 +/- 0.17% ID/g at 30 min and 2.40 +/- 0.17 vs 0.24 +/- 0.03% ID/g at 2 hr, respectively, p<0.01). However, the tumor uptake of both radiolabels were not significantly different at 24 hr (0.04 +/- 0.01 vs 0.05 +/- 0.01% ID/g). Tumor was visualized as early as at 30 min in gamma camera images. CONCLUSION: These data suggested that [131I]OMIMT might be a useful tumor imaging agent and has more advantage for the tumor imaging compared to [131I]IMT.
Animals
;
Gamma Cameras
;
Gliosarcoma
;
Rats*