Introduction: This study aims to quantify the concentrations of Cu, Cr, Zn, Ni, Pb, As and Cd in paddy soil and water and assess their potential dermal health risk to the farmers at Kampung Sawah Sempadan, Malaysia. Methods: 72 water samples and 72 soil samples were collected and analysed using inductively coupled plasma mass spectrometry (ICP-MS). 117 respondents were interviewed using a questionnaire to obtain the exposure information for dermal health risk assessments. Results: All elements in water did not exceed the recommended concentration by Malaysia National Water Quality Standard (NWQS) and Food and Agriculture Organization (FAO). Nevertheless, the maximum concentration of As (31.49 mg/kg) in paddy soil exceeded the Dutch Target Value for soil protection (29 mg/ kg). There was no significant chronic non-carcinogenic health risk for farmers working in the paddy soil and water (HQ<1, HI<1). The carcinogenic health risk of As was in the acceptable risk level (10-6 to 10-4). Conclusion: The contamination of the selected heavy metals in the paddy soil and water at Kampung Sawah Sempadan were lower than the available standards except for As in soil which exceeds the recommended value by Dutch target value. The health risk to these heavy metals were also minimal and within the acceptable level.
Water Quality

Introduction: Extensive agriculture activities in the upstream area causes pollutants flow to the downstream area and contaminate the drinking water resources. Methods: Water samples were collected from 27 sampling points of Bertam River in Cameron Highlands, during wet season in September 2017 for physicochemical properties and heavy metals analyses. Potential health risk was calculated based on the heavy metals concentration detected. Results: The pH value of the river ranged from neutral to slightly acidic (6.15-7.01). The overall mean level of turbidity (109.94 ± 160.73 NTU), DO (7.86 ± 0.71 mg/L), E. coli (5191.00 ± 14937.42 CFU/100 mL), and NH3-N (0.85 ± 0.54 mg/L) were exceeded the National Standard for Drinking Water Quality (NSDWQ) (Ministry of Health Malaysia). The concentration of heavy metals were in the following order; Fe>Al>Cu>Zn>Pb>Cd. The overall mean concentration of Cd (0.015 ± 0.007 mg/L), Fe (0.442 ± 0.191 mg/L) and Pb (0.021 ± 0.005 mg/L) were exceeded the national standard (NSDWQ). The non-carcinogenic health risk for adult and children from daily water consumption was within an acceptable risk. There is a potential cancer risk through Cd exposure in the drinking water where the risk higher among children (4.92 x 10-3), followed by adult male (3.06 x 10-3) and adult female (2.98 x 10-3). Conclusion: Significant heavy metals contamination and health risk through water consumption were observed in the highland river watershed, possibly due to the anthropogenic activities.
Water quality

Aims: Water is described as safe and wholesome when it is free from pathogenic microorganisms and chemical substances that are hazardous to human health. This study aimed to investigate the microbial quality of water used for drinking, cooking, bathing and other purposes at universities in Nigeria. Methodology and results: Water samples were collected from forty-four storage tanks across four selected universities. Total viable bacteria in the water samples were cultivated using the plate count agar. The isolation of total coliform and Escherichia coli were carried out on Harlequin™ E. coli/coliform agar (HA) medium, while media-faecal coliform was used for faecal coliform employing the membrane filtration technique. Physicochemical parameters such as alkalinity, pH, total alkalinity, total dissolved solid, total suspended solid, electrical conductivity, total hardness, fluoride and chloride ion concentrations, were evaluated in accordance with standard procedures. Data were compared statistically using MedCalc statistical software. Considering the heterotrophic bacterial counts, all water samples were unsatisfactory. For the total coliform counts, 50% of samples were satisfactory but suspicious, while remaining 50% were unsatisfactory. Faecal coliforms results showed that 50% of samples gave excellent quality, 25% showed satisfactory but suspicious quality, while 25% showed unsatisfactory result. There were no significant differences in the total viable, total coliform and E. coli counts of water sampled from universities A and D (p>0.05). The predominant bacterial species was Pseudomonas aeruginosa (23.17%), while the least encountered was Salmonella typhimurium (2.44%). All physicochemical parameters tested were within the acceptable limit. Water samples were collected from forty-four storage tanks across four selected universities. Total viable bacteria in the water samples were cultivated using the plate count agar. The isolation of total coliform and Escherichia coli were carried out on Harlequin™ E. coli/coliform agar (HA) medium, while media-faecal coliform was used for faecal coliform employing the membrane filtration technique. Physicochemical parameters such as alkalinity, pH, total alkalinity, total dissolved solid, total suspended solid, electrical conductivity, total hardness, fluoride and chloride ion concentrations, were evaluated in accordance with standard procedures. Data were compared statistically using MedCalc statistical software. Considering the heterotrophic bacterial counts, all water samples were unsatisfactory. For the total coliform counts, 50% of samples were satisfactory but suspicious, while remaining 50% were unsatisfactory. Faecal coliforms results showed that 50% of samples gave excellent quality, 25% showed satisfactory but suspicious quality, while 25% showed unsatisfactory result. There were no significant differences in the total viable, total coliform and E. coli counts of water sampled from universities A and D (p>0.05). The predominant bacterial species was Pseudomonas aeruginosa (23.17%), while the least encountered was Salmonella typhimurium (2.44%). All physicochemical parameters tested were within the acceptable limit. This study revealed that the water used by students of studied universities was contaminated with potential bacterial pathogens. However, all physicochemical parameters tested were within the permissible standard limits and satisfied the requirements for domestic utility.
Water Quality

Study on 124 samples of surface water at An Bien, Hon Dat and Tan Hiep districts, Kien Giang province to assess the quality based on biological-chemiphysical index. The situation of polluting the surface water at 3 districts were very serious. Most samples of water hadn't achieved the biological-chemiphysical index. 100% samples of water had examined at 3 areas had Fe content higher than permissible standards. 100% samples of water at Hon Dat had NOZ content higher than permissible standards, at An Bien was 29.5%. 100% samples of surface water at Hon Dat and Tan Hiep had faecal coliform content and E.coli higher than permissible standards.
Water ; Quality Control ; Water Pollution

The information of species and quantity of enteric viruses in surface water, finished water, and tap water is important in helping understand the pathogenesis of viruses, providing information about health and hygiene, improving handling technique of drinking water, and establishing the standards of water quality. Using standard total culturable virus assay-most probable number (TCVA-MPN) method, we tried to detect infectious enteric viruses in surface water, finished water, and tap water samples that were collected and evaluated according to the information collection rule (ICR). The results obtained with TCVA method were compared to the results from both reverse transcription-polymerase chain reaction (RT-PCR) and integrated cell culture-RT-PCR (ICC-RT-PCR) method. Five of 86 samples (5.8%) were positive as determined by the TCVA-MPN method. Two of 86 samples (2.3%) were positive for reovirus as determined by the RT-PCR and ICC-RT-PCR, and contained infectious reovirus. One of 86 samples (1.7%) was positive for coxsackievirus type B3 as determined by the RT-PCR and ICC-RT-PCR.
Drinking Water ; Hygiene ; Water Quality ; Water*

The speed of development in antibiotics appears have not kept pace with that of development in antibiotic-resistant bacteria. The appropriate antibiotic regimens will cure diseases and prevent the antibiotic-resistant bacteria from development. 4 antibiotic regimens were used randomly, included 1) penicillin alone; 2) penicillin combined with gentamycine, 3) cephalosporine; and 4) chloramphenicol. Regimens 2 and 3 provided good effect in treatment of severe and very severe pneumonia caused by antibiotic-resistant bacteria in under 5-year children (the rate of cure is 66.7% and 72%, respectively). Regimens 1 and 4 provided less effect (30.8% and 29%, respectively
Water ; Quality Control

The results of analysis showed that the pH were <6.5 or >8.5 in 45% of drinking water samples. The rate of drinking water samples that NO2 concentrations exceeded the set standards were between 10.9% and 30% in 3 districts (An Bien, Hon Dat, and Tan Hiep). The concentrations of organic substances and NH4 also exceeded 0-15% of standards, and many samples were substandard about microorganism such as E.coli, faecal coliform (15%) with p<0.05. It’s necessary to survey and study especially the local fact in building bore-well.
Water ; Epidemiology ; Quality Control

Aims: To determine abundance of potential pathogenic microorganisms in pangasius and tilapia farms in five major fish-producing areas in Bangladesh by PCR approaches. Methodology and results: Important microbial water quality indicators were studied in water of 38 fish farms producing pangasius (Pangasianodon hypophthalmus) and tilapia (Oreochromis niloticus) in five major fish-producing areas of Bangladesh. The parameters included physicochemical data and PCR detection of total coliforms and E. coli, species of potentially pathogenic Vibrio, and cyanobacterial genes encoding the toxins microcystin and saxitoxin. Quantitative PCR showed that coliform bacteria occurred in all fish farms with densities from one to 2.2 × 105 per mL, while E. coli ranged from none to 5.0 × 104 per mL. Numbers of total coliforms and E. coli were higher in pangasius farms than in tilapia farms, and when high abundances occurred, coliform bacteria and E. coli bacteria co-varied. Detection of Vibrio-specific genes indicated presence of Vibrio species in 76% of the farms and included V. vulnificus and V. cholerae. The human pathogen type of V. cholerae (carrying the ctxA gene) and the fish pathogen V. parahaemolyticus were not detected. The microcystin-encoding mcyE gene ranged from undetectable to 2.6 × 105 copies per mL and tended to be highest in pangasius farms. The saxitoxin-encoding gene sxtA was not found in any of the farms. Conclusion, significance and impact of study Based on the high abundance of especially coliform bacteria and E. coli, we recommend more efficient water quality monitoring systems to improve detection and control of fecal coliforms and to reduce presence of microcystin-producing cyanobacteria in aquaculture farms in Bangladesh
Water Quality ; Tilapia ; Catfishes

Background: A research on the quality and the use of water for living in order to discover solutions to ensure the supply of fresh water is urgent and necessary. It is also a basis to help policy makers to implement the right plan in supplying water for each region. Objectives: This research aims to evaluate the quality and the usage of water for living in several ecological regions of Viet Nam. Subjects and method: A descriptive, cross sectional study conducted in 7 chemical and physical indices and 2 microbiological indices in 1479 samples of waters that are used in 4 ecological regions of Vietnam. Results:The whole of the country has a high coverage of clean water (88.4%), but only 22.8% of sample meets the requirements. Especially, most of the samples do not meet the microbiological requirements. Conclusion: In general, the pollution level of the sources of water is at a medium level. The related factor that affects the clean water standard is the polluted sources under 10m nearby the polluting site.
quality and the use of water ; ecological regions

Objectives: The authors considered the community-based on-site water sampling-surveys case-study in Angono, Rizal, in the Republic of the Philippines. The study was designed to determine whether individual citizens within the community could successfully collaborate in order to develop a method for quantification of E. coli levels in water (especially underground water) for domestic use.

Methods: We used techniques that had been validated in Japan for on-site evaluation of E. coli. These simple techniques enabled the on-site quantification of E. coli levels in multiple water sources, including underground water.

Results: The techniques used in Japan were successfully adopted in the Philippines, and we cooperated with regional medical technicians, scientists, and citizens, in order to develop a system where ultimately, the people in Angono can perform all the tasks related to on-site water quality evaluation. From societal and economic perspectives, we were able to reduce the traditionally high laboratory cost (including testing cost and samples transit cost) of coliform bacteria and E. coli evaluation to a lower, fixed cost level that is affordable for regional health centers and other sites.

Conclusion: The transfer of a simple inspection method technology, under the supervision of an experienced Japanese research team, allowed us to validate the E. coli on-site quantification strategy at a regional level as "co-creation of science and society". In addition, beyond technology transfer, our collaborative efforts with scientists, regional medical engineers, and citizens, led to the creation of an NGO (non-governmental organization) that serves citizens in the town of Angono with regard to water quality assessment and certification activities.

Key Words: water quality, water management, community, local people, participatory approach, underground water, Escherichia coli, coliform bacteria, Philippines