1.Antibacterial and anti-biofilm activity of the lipid extract from Mantidis ootheca on Pseudomonas aeruginosa.
Wen-Dong WANG ; Nan-Nan ZHANG ; Warren CHANDA ; Min LIU ; Syed Riaz Ud DIN ; Yun-Peng DIAO ; Lei LIU ; Jing CAO ; Xiao-Li WANG ; Xing-Yun LI ; An-Hong NING ; Min HUANG ; Min-Tao ZHONG
Journal of Zhejiang University. Science. B 2018;19(5):364-371
The aim of this study is to assess the antibacterial and anti-biofilm properties of the lipid extract from Mantidis ootheca against the gentamycin resistant Pseudomonas aeruginosa. The chemical composition of the lipid extract and its relative proportion were determined using the technique of gas chromatography coupled with mass spectrometry (GC-MS). Antibacterial susceptibility tests were performed using a disc diffusion assay and the minimum inhibition concentration (MIC) was determined by way of the agar dilution method. The anti-biofilm test was carried out with crystal violet staining and scanning electron microscopy (SEM). There were 16 compounds detected, and the most abundant components were sesquiterpenoids, monoterpenes, and trace aromatic compounds. The MIC for P. aeruginosa was 4 mg/ml and the eradication effect on preformed biofilms was established and compared with a ciprofloxacin control. The results of our study indicated that a lipid extract from M. ootheca could be used as a topical and antibacterial agent with anti-biofilm activity in the future.
Animals
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Anti-Bacterial Agents
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pharmacology
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Biofilms
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drug effects
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Gas Chromatography-Mass Spectrometry
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Mantodea
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chemistry
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Microbial Sensitivity Tests
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Pseudomonas aeruginosa
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drug effects
2.Effectiveness of omega-3 polyunsaturated fatty acids against microbial pathogens.
Warren CHANDA ; Thomson P JOSEPH ; Xue-Fang GUO ; Wen-Dong WANG ; Min LIU ; Miza S VUAI ; Arshad A PADHIAR ; Min-Tao ZHONG
Journal of Zhejiang University. Science. B 2018;19(4):253-262
Microorganisms provide both beneficial and harmful effects to human beings. Beneficial effects come from the symbiotic relationship that exists between humans and microbiota, but then several human illnesses have turned some friendly microbes into opportunistic pathogens, causing several microbial-related diseases. Various efforts have been made to create and utilize antimicrobial agents in the treatment and prevention of these infections, but such efforts have been hampered by the emergence of antimicrobial resistance. Despite extensive studies on drug discovery to alleviate this problem, issues with the toxicity and tolerance of certain compounds and continuous microbial evolution have forced researchers to focus on screening various phytochemical dietary compounds for antimicrobial activity. Linolenic acid and its derivatives (eicosapentaenoic acid and docosahexaenoic acid) are omega-3 fatty acids that have been studied due to their role in human health, being important for the brain, the eye, the cardiovascular system, and general human growth. However, their utilization as antimicrobial agents has not been widely appreciated, perhaps due to a lack of understanding of antimicrobial mechanisms, toxicity, and route of administration. Therefore, this review focuses on the efficacy, mechanism, and toxicity of omega-3 fatty acids as alternative therapeutic agents for treating and preventing diseases associated with pathogenic microorganisms.
Animals
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Animals, Genetically Modified
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Anti-Infective Agents/chemistry*
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Antioxidants/chemistry*
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Bacterial Infections/microbiology*
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Cell Membrane/drug effects*
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Clinical Trials as Topic
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Docosahexaenoic Acids/chemistry*
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Drug Resistance, Bacterial
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Eicosapentaenoic Acid/chemistry*
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Fatty Acids, Omega-3/chemistry*
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Fishes
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Humans
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Lipids/chemistry*
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Mice
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Microbiota
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Rats
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alpha-Linolenic Acid/chemistry*