Objective To establish a continuous flow analytical method for determination of potassium in environmental water sample.Methods The concentration of patassium in environmental water sample was determined by ALLIANCE auto-matic analyzer,and its data were compared with the data obtained from atomic absorption spectrophotometry.Results The de-tection limit of this method was0.5?g /ml.The method revealed linear relationship at the potassium concentrations of5-1000?g /ml.The recovery rate of standard addition method ranged from to97%to102%.The relative standard deviations were0.82%-1.23%.Conclusion The contents of potassium in environmental water samples could be accurately and rapidly deter-mined by continuous flow analysis.

Objective To investigate the relationship between the changes of blood lipid 4 indexes and lactic acid level with prog‐nosis in children patients with septicopyemia .Methods Ninety‐five children patients with septicopyemia in our hospital from Janu‐ary 2013 to December 2014 were selected ,including 30 death cases(death group and) and 65 survival cases(non‐death group) .To‐tally 60 age‐and gender‐matched healthy children were enrolled as control group .The levels of serum cholesterol (TC) ,triglyceride (TG) ,high density lipoprotein cholesterol (HDL‐C) ,low density lipoprotein cholesterol(LDL‐C) and arterial blood lactate acid (LAC) were measured ,and the results were compared among the three groups .Results The TC and HDL‐C levels in the death group and the non‐death group were lower than those in the control group (P<0 .05) ,the LDL‐C level of the death group was low‐er than that the control group and survival group(P<0 .05) ,while the TG level in the death group and the non‐death group was higher than that in the control group(P<0 .05) ,moreover the TG level in the death group was increased significantly (P<0 .01) . In the excessive inflammation reaction ,the levels of TC ,HDL‐C and LDL‐C were dropped ,which were also decreased with the dis‐ease development ,but the TG level was increased .The LAC level in the death group was higher than that in the control group and the non‐death group ,which in the non‐death group was recovered to the normal level with the disease condition improvement ,while which in the death group was increased with the disease condition progression ,moreover the difference between on 1 d and 3 d had statistical difference(P<0 .05) .Conclusion Monitoring the levels of the blood lipid and lactic acid has an important significance to judge the severity and prognosis in children patients with septicopyemia .

Objective To study the effect of tripterygium glycosides combined with prednisone in the treat-ment of adult patients with purpura nephritis.Methods 20 adult patients with purpura nephritis were selected and randomly divided to two groups according to the random number table method.10 cases of the control group were administered with prednisone,10 cases of the observation group were administered with tripterygium glycosides and prednisone.After treatment,the treatment effect and postoperative recovery of the two groups were compared.Results The markedly effective,effective,ineffective,the total effective rates of the observation group were 5 cases(50%), 40 cases(40%),1 case (10%),9 cases (90%),respectively,which of the control group were 3 cases (30%), 4 cases(40%),3 cases(30%),7 cases(70%),there was statistically significant difference in the total effective rate between the two groups(χ2 =4.32,P <0.05);The disappeared time of negative urinary protein,hematuria,edema disappeared,negative hypertension in the observation group were (31.5 ±5.6)d,(35.6 ±6.6)d,(29.6 ±6.4)d, (30.3 ±6.5)d,respectively,which of the control group were (37.5 ±6.5)d,(42.6 ±8.2)d,(35.6 ±7.5)d, (37.7 ±7.3)d,the differences were statistically significant between the two groups(t =2.57,2.62,2.60,2.81,all P <0.05 ).The gastrointestinal discomfort,decreased white blood cells,abnormal liver function,total incidence of adverse reaction rates of the observation group were 1 case(10%),1 case(10%),1 case(10%),3 cases(30%), which of the control group were 1 case(10%),1 case(10%),0 case(0%),2 cases(20%),there was no statistically significant difference in the total incidence of adverse reaction rate between the two groups(χ2 =1.13,P <0.05). Conclusion In the treatment of adult patients with purpura nephritis,tripterygium glycosides combined with predni-sone has good effect,and can improve the symptoms of patients required a shorter time.

Objective To explore the effect of hemodialysis filtration and blood perfusion in the treatment of patients with uremic encephalopathy.Methods 30 patients with uremic encephalopathy were selected,they were treated with hemodialysis filtration and blood perfusion.After the treatment,the treatment effect and postoperative recovery of patients were compared.Results In the treatment,1 case coma for 5 days,after 2 times treatment with death;1 case coma for 7 days.After 3 times the combined treatment did not restore sanity;12 patients of combined treatment after 1 time of the symptoms disappeared completely;After 3 times of treatment on the patients with residual symptoms have less.Consciousness of patients was 25 cases (83.3%).Before treatment,BUN was (28.4 ± 3.1)mmol/L,Scr was (749 ±99.6)mol/L,PTH was (245.7 ±35.2)pg/g,K + was (4.7 ±1.4)mmol/L,Na + was (137.7 ±18.4)mmol/L,Cl -was (90.8 ±16.7)mmol/L,β2 -MG was (25.4 ±3.4)mg/L.After treatment,BUN was (9.1 ±2.4)mmol/L,Scr was (199.7 ±99.2)μmol/L,PTH was (105.6 ±33.6)pg/g,K + was (4.3 ± 1.3)mmol/L,Na + was (135.7 ±14.8)mmol/L,Cl - was (85.0 ±5.2)mmol/L,β2 -MG was (13.4 ±3.1)mg/L. The differences were statistically significant (all P <0.05).Conclusion In the treatment of patients with uremic encephalopathy,hemodialysis filtration and blood perfusion have good effect,should be used in clinical promotion.

The transcription factor of ethylene responsive factor binding protein (ERF) is belonged to AP2/ERF superfamily, which is known to be unique in plants. AP2/ERF proteins have important functions in the transcriptional regulation of a variety of biological processes related to growth and development, as well as various responses to environmental stimuli. An ERF gene from Salvia miltiorrhiza is cloned and divided into ERF gene family group VII of Arabidopsis and Rice. It contains a MCGGAI (I/L) motif referred to as CMVII-1 and a single intron in the 5'-flanking region of the AP2/ERF domain. Sequence analysis reveals that the region of second extron has abundant polymorphism sites. There are 21 single nucleotide polymorphism sites (SNPs) in the 264 bp region, among them, 14 SNPs are synonymous substitutions and 7 SNPs are non-synonymous substitutions. Though analysis of 181 samples from Shandong, Shaanxi and Sichuan Provinces, it reveals that each production area has its own special genotypes, 5 SNPs show significant difference. Cluster based on UPGMA method reveals that different populations from specific province have clustered together. It shows that SmERF gene will be a candidate molecular marker for the identification of Salvia miltiorrhiza from different areas.

Objective To study the changes of life span and pathology in superoxide dismutase 1 (SOD1)-G93A mice after intracardiac transplantation of human mesenchymal stem cells (hMSCs).Methods hMSCs were isolated from bone marrow cells obtained from healthy donors and cultured.The purity and morphology were assessed by flow cytometry (FCM).hMSCs (3×10~6) resuspended in 0.2 ml DMEM was injected into the heart of 8 week-old SOD1-G93A mice.In non-transplantion control SOD1-G93A mice, only DMEM was injected.The mice were evaluated for signs of motor deficit with 4-point scoring system previously described by Weydt et al.The age of onset and life span in mice were assessed.The pathological change including number of motor neurons was investigated by Nissl staining.Immunofluorescence staining with specific human nuclear antibody was used to confirm the transplant of hMSCs in mice.Results The onset symptoms in untreated SOD1-G93A mice appeared at (156.56±3.60) days of age and the average life span was (188.32±3.51) days.hMSCs transplantation delayed the onset of ALS type symptoms about 16 days (x~2=10.888, P=0.001) and prolonged the life span about 14 days compared to the untreated SOD1-G93A littermates((202.19±4.09) days vs (188.32±3.51) days, x~2=3.917, P=0.04).The loss of motor neurons in untreated mice was earlier and more severe than in hMSCs transplanted mice.At 20 weeks, the number of motor neurons in transplanted mice was significantly higher than those in untreated mice.Human specific nuclear antigen in brain and spinal cord was detected in transplanted SOD1-G93A mice.Conclusion hMSCs can be implanted for a long-term into central nervous system by intracardiac transplantation and the transplantation can prolong life span, and delay the onset of the disease and motor neuron loss in SOD1-G93A mice.

This study was aimed to establish a fast determination method of main components in honey. Honey samples from difference production bases were used as study objects. Transmission and reflection spectra of different honey samples were collected with the Fourier transform near infrared spectrometer. The main components in honey (moisture content, fructose content, glucose content and reducing sugar content) were detected by the near infrared quantitative analysis technique. The near infrared quantitative analysis models of moisture content, fructose content, glucose content and reducing sugar content in honey were established by the partial least squares (PLS). The results showed that the correlation coefficient (r) of the moisture content, fructose content, glucose content and reducing sugar content in honey were 0.997 25, 0.973 90, 0.927 94 and 0.952 68, respectively. The root mean square error of prediction (RMSEP) were 0.165 (%), 0.564 (%), 1.300 (%) and 1.270 (%), respectively. It was concluded that determination of main components in honey by the near-infrared spectroscopy technology was a fast and nondestructive determination method with high accuracy, which can be used in the quantitative detection of main components in honey.

This study was aimed to revise and improve the quality standard of Ping-An (PA) Pill. Fructus CaryophylliandFructus Aurantii ImmaturuswereidentifiedbyTLC.Thecontentofcostunolideand dehydrocostunolide were determined by HPLC. The results showed that there were clear spots, good degree of separation, and no negative interference in the TLC identification. The calibration curve of costunolide and dehydrocostunolide were linear at the range of 0.103 4-1.033 5μg (r=0.999 9) and 0.110 1-1.100 5μg (r=0.999 7). The average recoveries were 95.1% (RSD = 2.62%, n = 6) and 98.6% (RSD = 2.84%, n = 6), respectively. It was concluded that the method was convenient and accurate with strong specificity in the quality and quantity control of PA pill, which can be used in the quality control of PA pill.

Saussurea medusa is a rare traditional Chinese medicinal herb. Besides anti-inflammatory and analgesic activities, it has effects of disinhibiting cold, dispelling dampness and promoting blood circulation. Flavonoids are the main medicinal compounds in S. medusa. Contents of flavonoids and expression of flavonoids biosynthesis related genes in white and red (induced by low temperature, high sucrose and high light) callus were analyzed. The results showed that the total flavone in red line was 3.60 times higher compared to white line. The accumulation of rutin in red line (0.25% of dry weight) was 2.40 times higher compared to white line. Anthocyanins were abundant in red line, with the contents of cyanidin 3-O-glucosidechloride and cyanidin 3-O-succinyl glycoside 0.12% and 0.19% of dry weight respectively. CHS, F3'H, FNS, FLS, DFR and ANS genes were highly expressed in red line compared to white line. Expression of three transcription factors (MYB, bHLH and WD40) in red line was significantly higher than that in white line, especially the expression of MYB (19.70 times higher compared to white line). These results indicated that high expression levels of transcription factors induced high expression of structural genes in red line, thereby enhancing the flavonoids biosynthesis. The expression of bHLH and WD40 was similar, whereas it was significantly different from that of MYB, indicating that bHLH and WD40 could form a binary complex to regulate expression of structural genes and flavonoids biosynthesis.
Anthocyanins ; chemistry ; Cell Line ; Flavonoids ; chemistry ; Glucosides ; chemistry ; Plant Proteins ; genetics ; metabolism ; Plants, Medicinal ; chemistry ; Saussurea ; chemistry ; genetics ; Transcription Factors ; genetics ; metabolism

Objective To develop a convenient, rapid and specific method using real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) for detection of facioscapulohumeral muscular dystrophy(FSHD). Methods Genomic DNA was extracted and digested by restricted endonuclease EcoR Ⅰ , followed by agarose electrophoresis. The DNA (< 38 kb) was retrieved from agarose electrophoretic gels. The primers and probe were designed in D4ZA gene in chromosome 4. One hundred and fifteen subjects were examined by FQ-PCR using the retrieved DNA (<38 kb) as a template and the result was analyzed by fluorescent curve comparing with positive control. Results The results by FQ-PCR showed that 13 cases were positive in 16 FSHD cases whose EcoR Ⅰ fragment sizes were known, 75 cases were negative in 78 cases of normal controls, 15 cases were positive in 16 FSHD cases diagnosed clinically whose EcoR Ⅰ fragment sizes were unknown, and 3 cases were positive in 5 cases of relatives of FSHD patients. Consistency was checked using Kappa index between the 2 gene diagnostic tests for FSHD (FQ-PCR test and the traditional Southern blotting test), and between the 2 diagnostic criterions (gene diagnosis by FQ-PCR and clinical diagnosis). The results were statistically significant (κ = 0. 765, P = 0. 002 ; κ = 0. 844, P = 0. 000). Conclusions A new genetic diagnostic method of FSHD by FQ-PCR was developed, which was more simplified and reliable compared to the time-consuming, radioactive Southern blotting. It could also detect the D4Z4 arrays in cases having deletion of p13E-11 as well as the interchromosomal exchange between 4q35 and 10q26. The new method of FQ-PCR for FSHD may be extended to utilize clinically in future.