Objective : To analyze the association between multiple loci of genes and the risk of early⁃onset pre⁃ eclampsia (EOPE) with pregnancy induced hypertension. Methods : Among 382 EOPE patients who had lived in Yanbian area for more than 10 years , 192 patients were randomly selected as case group. At the same time , 192 cases of natural delivery in the hospital were randomly selected as the control group. PCR⁃RFLP method was used to determine the specific genotype and allele distribution information , and non⁃conditional Logistic method was used to obtain odds ratio (OR) and 95% confidence interval (CI) to confirm the risk of various genotypes. Results : There were two alleles of T and C at the GRB2 rs8082005 locus , TC , TT , and CC genotypes. There were two alleles of T and C at the RXRA rs3849222 locus , TC , TT , and CC genotypes. Through unconditional logistic regression analysis , in the GRB2 rs8082005 locus , compared with the TT genotype , the CC genotype was more susceptible to EOPE ( OR = 3. 155 , 95% CI = 1. 513 - 6. 579 , P = 0. 002) . In the explicit model , compared to patients with TT+ TC genotype , patients with CC genotype increased the risk of EOPE ( OR = 3. 000 , 95% CI = 1. 495 - 6. 022 , P = 0. 002) . In the RXRA rs3849222 locus , compared with the CC genotype , the TT genotype was more susceptible to EOPE ( OR = 2. 031 , 95% CI = 1. 077 - 3. 820 , P = 0. 028) . In the invisible model , compared to patients with CC + CT genotype , patients with TT genotype had an increased risk of EOPE ( OR = 2. 549 , 95% CI = 1. 421 - 4. 573 , P = 0. 002) . Conclusion There is a significant correlation between single nucleotide polymorphism (SNP) at the GRB2 rs8082005 and RXRA rs3849222 loci and the risk of EOPE in pregnant women with gestational hypertension in Yanbian area.

Objective : To investigate the relationship between the single nucleotide polymorphism of PPARG rs2290449 and the efficacy of combination therapy in sepsis patients with MODS． Methods : 382 cases of sepsis with MODS were selected and divided into the effective group and the ineffective group，and a case-control study was conducted．PCR-RFLP and Sequenom MassARRAY were used to detect the genotype and allele frequencies of the loci． Unconditional Logistic regression was used to calculate odds ratio ( OR) and 95% confidence interval ( CI) to evaluate the efficacy of combination therapy for sepsis with MODS in patients with different genotypes. Results: There were G and A alleles in PPARG rs2290449 locus，GG、GA and AA genotype．Non-conditional Logis- tic regression analysis showed that compared with the GG genotype group，the GA genotype group had a significant correlation with the efficacy of combination therapy for sepsis with MODS ( OR = 0. 449，95% CI = 0. 280－0. 722，P = 0. 001) ．In the dominant model，there was a significant correlation between the GA + AA genotype and the effi- cacy of combination therapy compared with the GG genotype ( OR = 2. 104，95% CI = 1. 332－3. 321，P = 0. 001) . After adjusting for confounding factors，unconditional Logistic regression analysis showed that compared with the GG genotype，the GA + AA genotype was significantly correlated with the efficacy of combination therapy in sepsis pa- tients with MODS ( OR = 2. 307，95% CI = 1. 438 －3. 701，P = 0. 001 ) ．Stratified analysis showed that compared with the population carrying GG genotype，the population carrying GA + AA genotype was significantly correlated with the efficacy of combination therapy in sepsis with MODS in the stratified analysis of age，gender and ethnicity. Conclusion PPARG rs2290449 single nucleotide polymorphism is significantly correlated with the efficacy of com- bination therapy in sepsis patients with MODS in Yanbian area.