Objective To analysis the development tendency and research focus of long-term care of chronic disease between China and abroad and guide the research and application of long-term care of chronic disease in China. Methods The PubMed database and China National Knowledge Infrastructure (CNKI) database were searched and co-word clustering analysis and multidimensional scaling analysis with Bicomb and SPSS 21.00 software were used. Results Foreign hot spots are summarized as follows:oxygen inhalation therapy; mental disorder patients; the needs and demands of long term care. And the following domestic hot spots: long term care for the aged; long term care insurance and social support. Conclusions Long-term care researches in China is still in the exploratory stage and combining with domestic and foreign research hot spots, proposal of long term care in China are summarized as follows:expand the study population of the domestic long-term care, such as the disabled and the mental disorder;enrich domestic long-term care research topics, such as long-term care model, caregivers, care quality, etc.

Objective Analysis the risk factors which maybe caused the cerebral hemorrhage after the liver transplantation, and then make out the counter nursing measures. Methods Selected 12 patients with cerebral hemorrhage after the liver transplantation as the experimental group, and then matching 48 patients without cerebral hemorrhage after the liver transplantation as the control group. Analysis the factor which related the cerebral hemorrhage. Results The factors which related the cerebral hemorrhage after the liver transplantation included in the Intensive Care Unite, heme level, Child level, APTT, albumin, blooding volume during the operation, operation time and the postoperative blood platelet level. The factors of Child level and the blooding volume during the operation were the independence risk factors of cerebral hemorrhage. Conclusion The factors of Child level and the blooding volume during the operation were the independence risk factors of cerebral hemorrhage, we should carry out counter nursing measures to prevent them.

OBJECTIVETo screen the allergens in patients with allergic rhinitis (AR) in Yibin, Zhaotong and some areas of the Changjiang River, and to study the distribution characteristics of inhaled allergens. METHODSThe clinical data of 661 patients with allergic rhinitis who diagnosed by skin prick test from March 2013 to June 2015 in the First people's Hospital of Yibin City were studied.RESULTSIn 661 patients, the top 5 allergens and their positive rates of skin prick test were Dermatophagoides farinae (76.5%), house dust mites (72.01%), Roach (35.85%), cat hair (11.35%) and dog hair (8.62%) respectively. There were no statistical difference of the positive rates of allergens among different seasons (χ2=4.568, 0.206). With the increasing age of the patients, the positive rate of allergens was decreased. CONCLUSION The dominated allergens in patients with AR are dust mites, house dust mites and cockroaches in Yibin, Zhaotong and and some areas of the Changjiang River. The prevention and control of AR in children should be toke more attention.

Objective To screen and identify the main pro-angiogenic compounds of Shexiang Baoxin pill ( SBP) presenting in the plasma.Methods The pro-angiogenic effects of SBP and its compounds absorbed into blood were measured by the cell prolif-eration and cell migration assays by xCELLigence .And the cell tube formation and rat aortic ring models were established to evaluate their pro-angiogenic effect.Results SBP(10 -4 ~10 -2 μg/ml), ginsenoside Rg3(1 ~10 μmol/L) and ginsenoside Rh2(1 ~10μmol/L)significantly stimulated human umbilical vein endothelial cells (HUVECs) proliferation, migration and tube-like structures formation at different concentrations (P<0.05).In addition, compared to the control group, only the high concentration group of SBP (10 -2 μg/ml), Rg3(10 μmol/L) and Rh2(10 μmol/L)could induce endothelial cell sprouting from the aortic ring(P<0.05) .Conclusion SBP, ginsenosideRg3 and Rh2 exhibited significantly pro-angiogenic effect in vitro.

Objective To build hypoxia/reoxygenation injury model in cultured neonatal rat cardiomyocyte and screen active components from Shexiang Baoxin Pill ( SBP) absorbed in blood against hypoxia/reoxygenation injury .Methods Cardiomyocytes were isolated and purified from hearts of neonatal Sprague Dawley rats (1~3 days old) and were used to build hypoxia/reoxygenation injury model.The components of SBP absorbed in blood were screened by methyl thiazolil tetracolium (MTT) colorimetic method.Results SBP showed significant protective effect against cardiomyocytes hypoxia /reoxygenation injury atthe concentration of 50 μg/ml.Ginsen-oside Rb1, Rb2, bufalin and muscone of twenty components from SBP absorbed in blood also possessed significant protective effect a -gainst cardiomyocytes hypoxia/reoxygenation injury .Conclusion SBP have the protective activity against cardiomyocytes hypoxia /reoxygenation injury , and ginsenoside Rb1, Rb2, bufalin, muscone are the main active components of SBP .This experiment offered basis for further pharmacodynamics and mechanism study of SBP .