1.To observe the curative effect of paracentesis and plaster mold compression fixation for the treatment of pseudocyst of auricle.
Xiaohui DU ; Jianxin YUE ; Wangyan CHEN
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2013;27(10):494-495
Adult
;
Casts, Surgical
;
Cysts
;
surgery
;
Ear Auricle
;
Female
;
Humans
;
Male
;
Middle Aged
;
Paracentesis
;
Treatment Outcome
2.Preventive effects of pueraria on presbycusis in rats
Wangyan CHEN ; Qi YAO ; Weihong LIU ; Bibo ZHANG ; Ying WANG ; Bo LIU
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2009;(15):703-705
Objective:To investigate the preventive effects of Pueraria on presbycusis in rats. Method:Thirty-two 24-26 month old Wistar rats were randomly divided into four groups, and were treated with different dosages of Pueraria (1,2,4,0 g·kg~(-1)·d~(-1)) seperately for 4 weeks. Auditory brainstem response (ABR) was used to de-tect the change of hearing threshold of rats. Hemorheological items of rats were checked in each group. Result: Compared with control group,the hearing threshold and hemorheological items of rats was significantly improved after treated with Pueraria(P<0. 05). In addition, 2 g/(kg·d) was found to be the best dosage of Pueraria for rats, which can achieve ideal effect with minimum side effect. Conclusion:Pueraria could improve tiny circulation, has good preventive effect on presbycusis of rats.
3.Comparation of low-temperature plasm radiofrequency and high-intensity focused ultrasound therapy on allergic rhinitis patients with nasal septal deviation.
Weihong LIU ; Chenrong WANG ; Qi YAO ; Ying WANG ; Wangyan CHEN
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2013;27(14):760-763
OBJECTIVE:
To compare the therapeutic effects of low-temperature plasm radiofrequency and high-intensity focused ultrasound in treatment of perennial allergic rhinitis (PAR) complicated with nasal septum deviation.
METHOD:
A total of 224 cases of identified PAR who treated in our hospital were randomly divided into 2 matched groups: low-temperature plasm radiofrequency group (n = 140) and high-intensity focused ultrasound group (n = 84). The therapeutic affection were evaluated by clinical symptoms controlled counting-scores. Inferior turbinate mucosa were collected and examined pathologically before and at 15 days and 6 months after the therapy.
RESULT:
The follow-up data indicated the effective rate in 15 days was 97.1% in high-intensity focused ultrasound group, and 90.4% in low-temperature plasm radiofrequency group, in 6 months was 90.7% and 85.4% respectively.
CONCLUSION
The treatment of high-intensity focused ultrasound in PAR is minimally invasive and safe with reliable efficacy.
Adolescent
;
Adult
;
Aged
;
Catheter Ablation
;
methods
;
Humans
;
Hypothermia, Induced
;
Middle Aged
;
Nasal Septum
;
abnormalities
;
Rhinitis, Allergic
;
Rhinitis, Allergic, Perennial
;
therapy
;
Ultrasonic Therapy
;
methods
;
Young Adult
4.The expression of COX-2 mRNA and protein in human laryngeal squamous cell carcinoma.
Wangyan CHEN ; Gang DENG ; Qi YAO ; Chengzhang YANG ; Bo LIU
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2008;22(12):532-535
OBJECTIVE:
To investigate the expression of COX-2 in both molecule and protein levels in laryngeal squamous cell carcinoma and the relationship between its intensity and histological grade, clinical stage and lymphoma metastasis.
METHOD:
By using emi-quantify RT-PCR and Immunohistochemistry, the expression of the COX-2 mRNA and COX-2 protein in laryngeal squamous cell carcinoma and polyp of vocal cord were examined.
RESULT:
Compared with the polyp of vocal cord tissue, the expression level of COX-2 mRNA and COX-2 protein in human laryngeal squamous cell carcinoma were significantly increased (P < 0.01). The intensity of COX-2 mRNA and protein expression increased as the laryngeal squamous cell carcinoma progresses developed.
CONCLUSION
COX-2 is high expressed in human laryngeal squamous cell carcinoma, and it may play an significant role in the growth, invasion and metastasis of human laryngeal squamous cell carcinoma. A combination of emi-quantify RT-PCR and immunohistochemistry is sensitive and specific for laryngeal neoplasms detection.
Adult
;
Aged
;
Carcinoma, Squamous Cell
;
genetics
;
metabolism
;
pathology
;
Cyclooxygenase 2
;
genetics
;
metabolism
;
Female
;
Humans
;
Laryngeal Neoplasms
;
genetics
;
metabolism
;
pathology
;
Male
;
Middle Aged
;
RNA, Messenger
;
genetics
5.Expression of Th1/th2 transcription factors and cytokines in laryngeal squamous cell carcinoma and its clinical significance.
Gang DENG ; Chengzhang YANG ; Wangyan CHEN
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2012;26(6):270-273
OBJECTIVE:
To study the differentiation of Th1/Th2 on the levels of transcription factors and cytokines production in patients with laryngeal squamous cell carcinoma in peripheral blood mononuclear cells (PBMC). In addition, the relation of p53 and T-bet, GATA3 expression was discussed for understanding the role of T-bet and GATA3 in metastasis.
METHOD:
The gene expression of Th1/Th2 type transcription factors T-bet, GATA3 and cytokines IFN-gamma, IL-4 were determined by RT-PCR and realtime RT-PCR from 49 patients with laryngeal squamous cell carcinoma and 30 normal controls. The expression of p53 was analyzed by immunohistochemistry.
RESULT:
Predominant expression of Th2 type cytokines was detected in 49 laryngeal squamous cell carcinoma patients. The expression rates of T-bet, GATA3, IFN-gamma and IL-4 was 42.86% (21/49), 71.43% (35/49), 26.53% (13/49), 63.27% (33/49) respectively. The expression rates of T-bet and IFN-gamma in patients were lower than in control group (P < 0.05), but the results of GATA3 and IL-4 were opposite (P < 0.05). The similar results were obtained by realtime PCR. The expression of p53 in patients was accompanied with lower expression of T-betmRNA and higher expression of GATA3mRNA.
CONCLUSION
There is predominant expression of Th2 type transcription factors and cytokines in PBMC of laryngeal squamous cell carcinoma patients. T-bet and GATA3 can be used as reference indicators for the metastasis of laryngeal squamous cell carcinoma.
Adult
;
Aged
;
Carcinoma, Squamous Cell
;
metabolism
;
pathology
;
Case-Control Studies
;
Female
;
GATA3 Transcription Factor
;
metabolism
;
Humans
;
Interferon-gamma
;
metabolism
;
Interleukin-4
;
metabolism
;
Laryngeal Neoplasms
;
metabolism
;
pathology
;
Leukocytes, Mononuclear
;
metabolism
;
Male
;
Middle Aged
;
RNA, Messenger
;
genetics
;
T-Box Domain Proteins
;
metabolism
;
Th1 Cells
;
metabolism
;
Th2 Cells
;
metabolism
;
Tumor Suppressor Protein p53
;
metabolism
6.Preventive effects of pueraria on presbycusis in rats.
Wangyan CHEN ; Qi YAO ; Weihong LIU ; Bibo ZHANG ; Ying WANG ; Bo LIU
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2009;23(15):703-705
OBJECTIVE:
To investigate the preventive effects of Pueraria on presbycusis in rats.
METHOD:
Thirty-two 24-26 month old Wistar rats were randomly divided into four groups, and were treated with different dosages of Pueraria (1, 2, 4, 0 g x kg(-1) x d(-1)) separately for 4 weeks. Auditory brainstem response (ABR) was used to detect the change of hearing threshold of rats. Hemorheological items of rats were checked in each group.
RESULT:
Compared with control group, the hearing threshold and hemorheological items of rats was significantly improved after treated with Pueraria (P<0.05). In addition, 2 g/(kg x d) was found to be the best dosage of Pueraria for rats, which can achieve ideal effect with minimum side effect.
CONCLUSION
Pueraria could improve tiny circulation, has good preventive effect on presbycusis of rats.
Aging
;
Animals
;
Auditory Threshold
;
Evoked Potentials, Auditory, Brain Stem
;
Hemorheology
;
Phytotherapy
;
Presbycusis
;
drug therapy
;
Pueraria
;
chemistry
;
Rats
;
Rats, Wistar