Objective:To explore the effect of health education nursing under the mode of graphic communication in blood donors.Methods:A total of 83 760 blood donors who performed unpaid blood donations at the Changzhi Center Blood Station of Shanxi Province from April 2017 to October 2019 were selected by convenient sampling method. Blood donors who underwent routine nursing from April 2017 to July 2018 were included in the control group ( n=42 075), and blood donors who underwent health education nursing under the mode of graphic communication from August 2018 to October 2019 were included in the study group ( n=41 685). The adverse effects of blood donation and the rate of re-donation within one year of the two groups of blood donors were compared. The Visual Analogue Scale (VAS) and Self-evaluation Blood Donation Questionnaire were used to compare the pain and psychological feelings of the two groups of blood donors. Results:The total incidence of adverse blood donation reactions, VAS score and Self-evaluation Blood Donation Questionnaire score of the study group were lower than those of the control group, and the blood donation rate within one year was higher than that of the control group, and the differences were statistically significant ( P<0.01) . Conclusions:Health education nursing under the mode of graphic communication can help reduce the incidence of adverse blood donation reactions of blood donors, increase the rate of re-donation within one year, relieve the pain of blood donation, and improve the psychological feelings of blood donors.