Objective To investigate the effects of genistein on genioglossus muscle function and nuclear factor erythroid 2 related factor 2(Nrf-2)/antioxidant responsive element(ARE) signaling pathway in rats under chronic intermittent hypoxia(CIH) condition in order to find medication treatment of obstructive sleep apnea/hyponea syndrome.Methods Thirty female Sprague-Dawley(SD) rats (8 weeks old) were randomly(random number table) divided into three groups 1 week after ovariectomy: control group(NC group), CIH group, and CIH+genistein treatment group(T group).Rats in the latter two groups were exposed to CIH for 8 h/d for 5 weeks.Electrophysiological method was used to detect the change of genioglossus muscle function, and real-time reverse transcription(RT)-PCR and Western blotting were used to determine the level of Nrf-2 gene and protein.Results Compared to NC group, the contractive properties of genioglossus muscle fatigue test at every time set was significantly decreased in CIH group(P＜0.05).Compared to CIH group, the contractive properties was significantly increased in T group(P＜0.05).The level of Nrf-2 gene and protein were less in CIH group(0.54±0.11 and 0.35±0.13) than in NC group(1.00±0.00 和 1.00±0.00)(P＜0.05).Compared to CIH group the level of Nrf-2 gene and protein were increased in T group (0.76 ± 0.16 and 0.63 ± 0.14)(P＜0.05), however, it was still less than the level in NC group(P＜0.05).Conclusions CIH attenuates genioglossus muscle fatigue resistance under chronic intermittent hypoxia through Nrf-2/ARE signaling pathway.Genistein protects genioglossus muscle function through up-regulation of the level of Nrf-2 gene and protein.

Objective To analyze the current cultural state of the hospital culture with multiple campuses,identify fac-tors influencing its development,and propose targeted strategies for integration and harmonization of culture across the hospital's branches.Methods The Cite Space was employed for the annual literature statistics and knowledge mapping of hospital culture of multiple campuses.A survey with a questionnaire was then conducted among 110 employees of a public dental hospital in Zhe-jiang Province.The factors influencing the development of culture were investigated.Results The volume of research literature on multi-campus hospital culture was observed to have been upsurge since 2020,with a rapid increase in the publications from 2020 to 2022.The knowledge map of keywords indicated a diversification in the research focuses on multi-campus public hospi-tals,with"cultural construction"emerging prominently between 2019 and 2020,with a burst strength of 1.47.Cultural construc-tion became a focal keyword in multi-campus hospital research.Pearson correlation analysis revealed multi-campus hospital cul-ture was significantly positively correlated with healthcare services,Party and group organization construction,image promotion,and human resource distribution across campuses(P＜0.01).Conclusion Research on the construction of culture in multi-campus hospital is gaining momentum.As hospitals continue to expand in scale,there is a need to strengthen the Party-led gov-ernance,diversify cultural construction efforts,strategically plan disciplines,manage medical quality through homogenized ap-proaches,accommodate differences within the cultural system,and establish a unified brand image.