1.Chemical constituents from Sarcandra glabra.
Chao WANG ; Liping ZHU ; Jingzhi YANG ; Chuangjun LI ; Dongming ZHANG
China Journal of Chinese Materia Medica 2010;35(6):714-717
OBJECTIVETo study the chemical constituents of the plant of Sarcandra glabra and provide reference for the study of the bioactive substances.
METHODThe compounds were isolated from the EtOH extract by various chromatographic methods and their structures were elucidated by their physico-chemical properties and the analysis of their spectral data.
RESULTNine compounds were isolated and identified as isoscopletin (1), syringaresinol monoside (2), styraxjaponoside B (3), 5-O-caffeoylshikimic acid (4), shizukanolide E (5), isoastilbin (6), neoisoastilbin (7), astilbin (8), neoastilbin (9).
CONCLUSIONCompounds 1-7 were isolated from S. glabra for the first time.
Cholestenones ; analysis ; Drugs, Chinese Herbal ; analysis ; chemistry ; Flavonoids ; analysis ; Flavonols ; analysis ; Furans ; analysis ; Lignans ; analysis ; Magnoliopsida ; chemistry ; Plant Bark ; chemistry ; Plant Stems ; chemistry ; Shikimic Acid ; analogs & derivatives ; analysis
2.Alternative Biological Material for Tissue Engineering of the Vagina: Porcine-Derived Acellular Vaginal Matrix
Yanpeng TIAN ; Yibin LIU ; Yanlai XIAO ; Zhongkang LI ; Mingle ZHANG ; Liang CHEN ; Zhen LI ; Wangchao ZHANG ; Zhiqiang ZHANG ; Desheng KONG ; Li MENG ; Yanfang DU ; Jingkun ZHANG ; Jingui GAO ; Xianghua HUANG
Tissue Engineering and Regenerative Medicine 2024;21(2):277-290
BACKGROUND:
Mayer-Rokitansky-Küster-Hauser (MRKH) syndrome is a severe congenital disorder characterized by vaginal hypoplasia caused by dysplasia of the Müllerian duct. Patients with MRKH syndrome often require nonsurgical or surgical treatment to achieve satisfactory vaginal length and sexual outcomes. The extracellular matrix has been successfully used for vaginal reconstruction.
METHODS:
In this study, we developed a new biological material derived from porcine vagina (acellular vaginal matrix, AVM) to reconstruct the vagina in Bama miniature pigs. The histological characteristics and efficacy of acellularization of AVM were evaluated, and AVM was subsequently transplanted into Bama miniature pigs to reconstruct the vaginas.
RESULTS:
Macroscopic analysis showed that the neovaginas functioned well in all Bama miniature pigs with AVM implants. Histological analysis and electrophysiological evidence indicated that morphological and functional recovery was restored in normal vaginal tissues. Scanning electron microscopy showed that the neovaginas had mucosal folds characteristics of normal vagina. No significant differences were observed in the expression of CK14, HSP47, and a-actin between the neovaginas and normal vaginal tissues. However, the expression of estrogen receptor (ER) was significantly lower in the neovaginas than in normal vaginal tissues. In addition, AVM promoted the expression of b-catenin, c-Myc, and cyclin D1. These results suggest that AVM might promotes vaginal regeneration by activating the b-catenin/cMyc/cyclin D1 pathway.
CONCLUSION
This study reveals that porcine-derived AVM has potential application for vaginal regeneration.