Objective To investigate the clinic significance of serum carbohydrate antigen ( CA)125 for cirrhosis. Methods 88 patients with type B posthepatitic cirrhosis (male 46,female 24,average age 45?24 years) were divided into three group (A stage35,B stage 28,C stage 25) according to Child-pugh integration. 36 healthy persons as normal controls were studied(male 20,female 16,average age 42?22 years). Serum CA125 was measured by ELISA. We investigated the alteration of serum CA125 with hepatic functional classification. Results The levels of serum CA125 in cirrhosis patients were markedly higher than those in control subjects(P

Toll-like receptor( TLR) ,which belongs to pattern recognition receptor,is type I transmem-brane glycoprotein. TLR activates natural immune and induces several cytokines. Foreign studies have showed that bacterial infection is closely related with TLR. In this paper,the relationship between TLR and bacterial in-fection is reviewd.

As an efficient gene transfer vehicle lentiviral vector has been widely used in therapy research. Comparing with other retrovirus vectors, lentiviral vectors have the unique ablility of transfecting nondividing cells and terminal differentiated cells. In addition, lentiviral vectors can accommodate two or more promoters and can carry larger foreign gene insertions. Now the new generation of lentiviral vectors encoding transcriptional control sequence provides effective means for the regulation of foreign gene expression. The development of lentiviral vectors and its application in the gene therapy field were summarized.

A pyelonephritis model of mouse is made by c. albicans infection. The determination of im-mune functions in model show that, both proliferation response of T cell to ConA and IL-2 activ-iting of spleenocytes of model animal to inductionwith ConA increased at 30 days after injected.The proliferation response of B cell to stimulation with LPS inhanced from 15 through 30 days,primary Ab response of model animal to SRBC stimulation inhanced between 15-30 days afterimmunization. DTH response to stimulation with c. albicans protein Ag raised since 15 days andup to peak at 30 days. Agglutination titer of serum raised up 15 days, and remained at higherlevel untill 30 days.

Objective To investigate characteristics of endometriotic lesions clone with various pathological subtypes by analyzing the polymorphism of X chromosome linked human androgen receptor allele (HUMARA). Methods Eight frozen tissues of endometriotic leisons were collected from 6 patients who received laparotomy or laparoscopy surgery in Peking Union Medical College Hospital from Nov. 2008 to Jun. 2009. Fifty specimens of epithelial cells from single endometrial glands were isolated and collected from endometriotic lesions by using laser capture microdissection. HUMARA was applied as the gene marker of clonal analysis. Nested palymerase chain reaction, double-enzyme digestion reaction with two methylationsensitive restriction endonuclease (Hha Ⅰ and Hpa Ⅱ), and the automated gene sequencing technique were utilized in this study to evaluate the characteristics of endometriotic lesions clone. Results Of 50 specimens of isolated glands, 34 were informative for clonal analysis, and all of which showed monoclonality. Of 3 ovarian endometriotic tissues, one tissue of HUMARA showed unuseful information, the other 2 ovarian endometriotic tissues respectively had 4 and 7 informative specimens of gland epithelial cells, and all of the glands from each tissue showed uniform clonal pattern. Two peritoneal endometriotic tissues had 1 and 5 informative specimens from individual glands, respectively; and the clonal patterns in 5 glands from the single lesion were divergent. Two abdominal wall endometriotic tissues had 7 and 6 informative specimens,respectively; and variable clonal patterns were seen in different glands from each lesion. One deep infiltrating endometriotic lesion had 4 informative specimens of isolated glands, and all of them showed unique clonal pattern. The disparate clonal patterns were found in endometriotic lesions with variable pathological subtypes, even arising from the same patient. Conclusions The epithelial cells from individual endometriotic gland showed monoclonality , and different glands from the same endometriotic lesion might show divergent patterns.

OBJECTIVE: To discuss the impact of Zero Inventory of Chinese decoction pieces in our hospital. METHODS: Zero Inventory of Chinese decoction pieces was analyzed from aspects of hospital resource utilization, pharmacy management, and quality assurance of decoction pieces. RESULTS: Zero Inventory can help save precious hospital resources, streamline pharmacy management and ensure quality of decoction pieces supply. CONCLUSION: Zero Inventory of Chinese decoction pieces has positive impact on hospital pharmacy management; therefore it deserves to be popularized.

Disease Notification ; Humans ; Internet ; Occupational Diseases

Objective To investigate the change in natural kill cell,T-ly mphocyte subset counts and effect of recovery after homologous and autologous blood transfusion.Methods 50 patients undergoing lung cancer operation were randomly divided into two groups:group A received autologous blood transfusion with 400ml and group H received homologous blood transfusion with 400ml.Venous blood samples were taken before surgery,before blood transfusion and on the 1st and 5th postoperative day for determination of T-ly mphocyte subsets and natural kill cell counts by flow cytomet.The existent ratio of 1～3 years were followed up.Results The NK cell,CD+_3 and CD+_4 counts and CD+_4/CD+_8 ratio decreased significantly on the 1st postoperative day in both groups(P

Objective To explore the causes of bile duct injury during laparoscopic cholecystectomy and its treatment.Methods From January 1998 to February 2007,totally 8600 cases of LC were performed in our hospital.Bile duct injury occurred in 36 of them,among which,the common hepatic duct was cut in 20 cases,the common bile duct was severed in 4 patients,the common hepatic duct was cut and freed in 4;incomplete clipping of the common hepatic duct or common bile duct occurred in 3 and 1 cases respectively;3 cases had injury to the conjunction of the cystic duct and common hepatic duct;electrical burn injury happened in 1 patient.Thirty one of the injured cases were detected during the laparoscopic surgery,while the other five were found because of jaundice or biliary leakage.The patients received bile duct repair and T tube drainage(24),T tube drainage(4),Roux-en-Y bowel biliary anastomosis(4),bile duct anastomosis(1),or removal of the titanium clips after the accidents.Results One of the patients who received bile duct anastomosis developed biliary stenosis after the T tube was withdrawn in 2 months after the surgery,Roux-en-Y bowel biliary anastomosis was thus performed 3 months later.Two patients who underwent Roux-en-Y anastomosis developed anastomotic stenosis after the operation,and thus received a second anastomosis in 11 months.These 3 patients who received totally 3 operations,were followed up for 2 to 3 years,none of them developed cholangitis or biliary stones.One patient in this series had refractory cholangitis,jaundice,and liver function impairment,and was cured after 1-year anti-inflammation and liver support therapy.In the other 32 patients,an 8-to 36-month follow-up(mean,16 months) was achieved,during which none of them showed any complications.Conclusions Bile duct injury can be due to lacking experience and knowledge of the complications of laparoscopic cholecystectomy,inappropriate enrollment of the patients,inaccurate operation,misuse of the surgical instruments,or being unable to discern the Calot's triangle.Strict indications for the operation,advanced training for young doctors,and appropriate timing for conversion to an open surgery are necessary to decrease the rate of bile duct injury.

Objective To evaluate the curative effect and security ofYangxue-Qingnao granules on posterior circulation ischemia.Methods 52 patients with posterior circulation ischemia from September 2012 to January 2014 in Remin hospital of Wuhan Universityk, were randomly recruited into a control group and a treatment group, with 26 cases in each group. The control group was only treated with flunarizine HCL and Betahistine Mesilate Tablets, while the treatment group was givenYangxue-Qingnao granules based on the control group. The change of clinical symptoms, TCD and cervical vascular ultrasound were observed before and after the treatment.Results After the treatment, the total effective rate of treatment group was significantly different to the control group (96.2%vs. 76.9%;χ2=4.127,P=0.042). The changes of blood flow velocity of vertebral artery, basilar artery and posterior inferior cerebellar artery were all decreased after the treatment in both groups (tvalue in the treatment group were 20.624, 13.773, and 9.485 respectively,t value in the control group were 13.203, 9.550, and 5.655, respectively, allP<0.01), besides such decreases in the treatment group were better than the control group (allP<0.01). After the treatment, the condition of carotid IMT decrease was significantly better in the treatment group than the control group (the number rate of IMT decrease was (96.2%vs. 69.2%;χ2=5.318,P=0.021).ConclusionYangxue-Qingnao granules can obviously improve the blood flow velocity and the thickness of IMT in patients with posterior circulation ischemia. The short-term curative effect is obvious.