Toll-like receptor( TLR) ,which belongs to pattern recognition receptor,is type I transmem-brane glycoprotein. TLR activates natural immune and induces several cytokines. Foreign studies have showed that bacterial infection is closely related with TLR. In this paper,the relationship between TLR and bacterial in-fection is reviewd.

Objective To investigate the clinic significance of serum carbohydrate antigen ( CA)125 for cirrhosis. Methods 88 patients with type B posthepatitic cirrhosis (male 46,female 24,average age 45?24 years) were divided into three group (A stage35,B stage 28,C stage 25) according to Child-pugh integration. 36 healthy persons as normal controls were studied(male 20,female 16,average age 42?22 years). Serum CA125 was measured by ELISA. We investigated the alteration of serum CA125 with hepatic functional classification. Results The levels of serum CA125 in cirrhosis patients were markedly higher than those in control subjects(P

As an efficient gene transfer vehicle lentiviral vector has been widely used in therapy research. Comparing with other retrovirus vectors, lentiviral vectors have the unique ablility of transfecting nondividing cells and terminal differentiated cells. In addition, lentiviral vectors can accommodate two or more promoters and can carry larger foreign gene insertions. Now the new generation of lentiviral vectors encoding transcriptional control sequence provides effective means for the regulation of foreign gene expression. The development of lentiviral vectors and its application in the gene therapy field were summarized.

A pyelonephritis model of mouse is made by c. albicans infection. The determination of im-mune functions in model show that, both proliferation response of T cell to ConA and IL-2 activ-iting of spleenocytes of model animal to inductionwith ConA increased at 30 days after injected.The proliferation response of B cell to stimulation with LPS inhanced from 15 through 30 days,primary Ab response of model animal to SRBC stimulation inhanced between 15-30 days afterimmunization. DTH response to stimulation with c. albicans protein Ag raised since 15 days andup to peak at 30 days. Agglutination titer of serum raised up 15 days, and remained at higherlevel untill 30 days.

Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; CD56 Antigen ; metabolism ; Cyclophosphamide ; therapeutic use ; Doxorubicin ; therapeutic use ; Esophageal Neoplasms ; drug therapy ; metabolism ; pathology ; radiotherapy ; Follow-Up Studies ; Humans ; Leukocyte Common Antigens ; metabolism ; Lymphoma, Extranodal NK-T-Cell ; drug therapy ; metabolism ; pathology ; radiotherapy ; Male ; Middle Aged ; Poly(A)-Binding Proteins ; metabolism ; Prednisone ; therapeutic use ; T-Cell Intracellular Antigen-1 ; Treatment Outcome ; Vincristine ; therapeutic use

Objective To evaluate the curative effect and security ofYangxue-Qingnao granules on posterior circulation ischemia.Methods 52 patients with posterior circulation ischemia from September 2012 to January 2014 in Remin hospital of Wuhan Universityk, were randomly recruited into a control group and a treatment group, with 26 cases in each group. The control group was only treated with flunarizine HCL and Betahistine Mesilate Tablets, while the treatment group was givenYangxue-Qingnao granules based on the control group. The change of clinical symptoms, TCD and cervical vascular ultrasound were observed before and after the treatment.Results After the treatment, the total effective rate of treatment group was significantly different to the control group (96.2%vs. 76.9%;χ2=4.127,P=0.042). The changes of blood flow velocity of vertebral artery, basilar artery and posterior inferior cerebellar artery were all decreased after the treatment in both groups (tvalue in the treatment group were 20.624, 13.773, and 9.485 respectively,t value in the control group were 13.203, 9.550, and 5.655, respectively, allP<0.01), besides such decreases in the treatment group were better than the control group (allP<0.01). After the treatment, the condition of carotid IMT decrease was significantly better in the treatment group than the control group (the number rate of IMT decrease was (96.2%vs. 69.2%;χ2=5.318,P=0.021).ConclusionYangxue-Qingnao granules can obviously improve the blood flow velocity and the thickness of IMT in patients with posterior circulation ischemia. The short-term curative effect is obvious.

OBJECTIVE: To investigate the effect of Salvia Miltiorrhiza injection (SMI) on peritoneal dialysis solution (PDS) induced injuries of peritoneal structure and function in a rat model, and to observe the relationship between the failure of peritoneal dialysis and expressions of aquaporin-1 (AQP-1) and zonula occluden-1 (ZO-1) in peritoneal tissues. METHODS: Fifty-six SD rats were randomly divided into normal control group, 1.5% PDS group, 4.25% PDS group, 1.5%PDS+1% SMI group, 1.5%PDS+2% SMI group, 4.25% PDS+1% SMI group and 4.25% PDS+2% SMI group. Two-hour peritoneal dialysis test was performed in rats in different groups by intraperitoneal injection for 8-week. Then rats were killed on the 8th week, and the bloods and peritoneal tissues were gathered. The rate of ultrafiltration, clearance rates of urea nitrogen, creatinine and glucose of peritoneum and content of total protein in PDS were detected. Peritoneal membrane histology was evaluated by light microscopy and transmission electron microscopy. Expressions of ZO-1 and AQP-1 proteins in peritoneal tissues were detected by immunohistochemical method, and AQP-1 protein expression was also detected by Western blotting technique. RESULTS: Compared with normal control group, using of 1.5% PDS and 4.25% PDS caused the changes of structure and function in peritoneum, such as pathological change of peritoneum, decreasing of the rate of ultrafiltration (P<0.05), clearance rates of creatinine and glucose (P<0.01) and the expression of ZO-1 protein (P<0.05), and increasing of the expression of AQP-1 protein (P<0.05). Compared with the simple PDS groups, the pathological damage of peritoneum was lessened and the rate of ultrafiltration and clearance rates of creatinine and glucose were increased in the 1.5% PDS+2% SMI group and 1.5% PDS+2% SMI group. Expression of AQP-1 protein was decreased by 1.5% PDS+2% SMI as compared with 1.5% PDS (P<0.05).[JP] CONCLUSION: SMI can relieve the injuries of function and structure of peritoneum by down-regulating the expression of AQP-1 protein.

Objective To investigate characteristics of endometriotic lesions clone with various pathological subtypes by analyzing the polymorphism of X chromosome linked human androgen receptor allele (HUMARA). Methods Eight frozen tissues of endometriotic leisons were collected from 6 patients who received laparotomy or laparoscopy surgery in Peking Union Medical College Hospital from Nov. 2008 to Jun. 2009. Fifty specimens of epithelial cells from single endometrial glands were isolated and collected from endometriotic lesions by using laser capture microdissection. HUMARA was applied as the gene marker of clonal analysis. Nested palymerase chain reaction, double-enzyme digestion reaction with two methylationsensitive restriction endonuclease (Hha Ⅰ and Hpa Ⅱ), and the automated gene sequencing technique were utilized in this study to evaluate the characteristics of endometriotic lesions clone. Results Of 50 specimens of isolated glands, 34 were informative for clonal analysis, and all of which showed monoclonality. Of 3 ovarian endometriotic tissues, one tissue of HUMARA showed unuseful information, the other 2 ovarian endometriotic tissues respectively had 4 and 7 informative specimens of gland epithelial cells, and all of the glands from each tissue showed uniform clonal pattern. Two peritoneal endometriotic tissues had 1 and 5 informative specimens from individual glands, respectively; and the clonal patterns in 5 glands from the single lesion were divergent. Two abdominal wall endometriotic tissues had 7 and 6 informative specimens,respectively; and variable clonal patterns were seen in different glands from each lesion. One deep infiltrating endometriotic lesion had 4 informative specimens of isolated glands, and all of them showed unique clonal pattern. The disparate clonal patterns were found in endometriotic lesions with variable pathological subtypes, even arising from the same patient. Conclusions The epithelial cells from individual endometriotic gland showed monoclonality , and different glands from the same endometriotic lesion might show divergent patterns.

Disease Notification ; Humans ; Internet ; Occupational Diseases

Codon ; Herpes Simplex ; virology ; Herpesvirus 1, Human ; genetics ; metabolism ; Humans ; Protein Binding ; Protein Biosynthesis ; Viral Proteins ; genetics ; metabolism