Objective To understand the knowledge,attitudes and impact factors related to voluntary counseling and testing(VCT)for HIV/AIDS among migrants in Minhang district of Shanghai.Method Using a convenience sampling strategy,74 migrants,27 key informants and 20 community service providers were selected to participate in in-depth interviews and focus group discussions.Results Most migrants were young,less educated and poorly aware of HIV prevention.Only one third of the migrants heard of VCT,but few of them knew where to seek VCT or had any personal VCT experience.Nearly half of the interviewees took a negative attitude towards those who participated in VCT service.The main factors influencing the participation in VCT service among the migrants were the knowledge of VCT,having risk behavior and perception,supportive environments and availability of HIV/VCT services.Conclusions There is an urgent need to promote extensive health education about HIV/VCT,adopt flexible and available approaches to VCT service and provide appropriate care following the HIV test among migrants.

Objective: To investigate the effect of helper T cells (Th1 and Th2) and its important significance in judging prognosis of patients with advanced lung cancer. Methods: Interferon-? (INF-?) and interleukin-4 (IL-4) in plasma and pleural effusion were detected by enzyme-linked immunoassay in patients with advanced lung cancer, IFN-? and IL-4 reflected activity of Thl and Th2 respectively. Results: The activity of Th1 in patients with advanced lung cancer was lower than that in normal persons in peripheral blood. For non-outstanding curative effect or progressive state of illness, the activity of Thl in patients of above 1 year survival time decreased in post-treatment than in pretreatment, the activity of Th2 increased in post-treatment. Conclusion: Activity of Helper T cells ( Th1, Th2 ) could be an important marker to diagnose lung cancer and judge prognosis in patients with advanced lung cancer.

Objective:To investigate the effect of different schemes of intrauterine insemination (IUI) on pregnancy outcome in patients with unexplained infertility.Methods:The clinical data of 393 cases (934 cycles) with unexplained infertility patients who underwent IUI from March 2016 to August 2018 in Dalian Maternal and Child Health Hospital were retrospectively analyzed. The patients were divided into natural cycle group (402 cycles) and promote ovulation cycle group (532 cycles). In promote ovulation cycle group, the patients were divided into clomiphene (CC) subgroup (124 cycles), letrozole (LE) subgroup (107 cycles), menotropins (HMG) subgroup (87 cycles), CC + HMG subgroup (103 cycles), LE + HMG subgroup (111 cycles). The pregnancy outcomes of each group were compared.Results:The clinical pregnancy rate and live birth rate in promote ovulation cycle group were significantly higher than those in natural cycle group: 14.10% (75/532) vs. 9.20% (37/402) and 11.28% (60/532) vs. 7.21% (29/402), and there were statistical differences ( P<0.05). There were no statistical differences in abortion rate, ectopic pregnancy rate and twin pregnancy rate between 2 groups ( P<0.05). Ovarian hyperstimulation syndrome occurred in 7 cycles, all of which were in CC + HMG subgroup. The clinical pregnancy rate and live birth rate in LE subgroup, HMG subgroup, CC + HMG subgroup and LE + HMG subgroup were significantly higher than those in CC subgroup: 11.21% (12/107), 13.79% (12/87), 16.50% (17/103), 21.62% (24/111) vs. 8.06% (10/124) and 7.48% (8/107), 11.49% (10/87), 13.59% (14/103) and 20.72% (23/111) vs. 4.03% (5/124), the abortion rate was significantly lower than that in CC subgroup: 3/12, 1/12, 2/17 and 1/24 vs. 5/10; the clinical pregnancy rate and live birth rate in HMG subgroup, CC + HMG subgroup and LE + HMG subgroup were significantly higher than those in LE subgroup, the abortion rate was significantly lower than that in LE subgroup, and there were statistical differences ( P<0.05). Conclusions:When the patients with unexplained infertility are assisted by IUI, LE combined with HMG ovulation induction can achieve a better pregnancy outcome.

The association between the polymorphism of SLC17A1 gene and hyperuricemia in Uyghur population in Xinjiang region was explored by Chi-square test.A total of 1 024 patients with hyperuricemia and 1 033 healthy volunteers were included.The genotype frequencies of CC,CT,and Tr in hyperuricemia and healthy controls were 24.9％,53.14％,21.96％ and 29.7％,47.52％,22.77％,respectively.There was statistical difference in SLC17A1 rs9467596 genotype frequencies between hyperuricemia cases and controls (x2 =7.492,P =0.024).CT genotype could increase the risk of hyperuricemia compared with CC genotype (OR =1.334,95％ CI 1.082-1.644).No statistical significance among the genotypes was found in age,body mass index,blood pressure,blood glucose,triglyceride,total cholesterol,High-density lipoprotein-cholesterol,low-density lipoprotein-Cholesterd,blood urea nitrogen,and Creatinine.The polymorphism of rs9467596 in SLC17A1 may be a genetic marker to assess risk of hyperuricemia among Xinjiang Uyghur population.

Purpose　The aim is to prepare the extract of immunocompetent cell proliferation from human placenta and to try to find out a suitable method of preserving the extract.Methods　The extract was mainly prepared by heat-treating placenta homogenated fluid. Then the activity to stimulate murine splenic lymphocyte proliferation in vitro was done with MTT , exposing　the　extract to　radioisotope 60　Co.Results　The content of protein was 2～3mg per gram placenta measured by Lowry′s Method.The rate to promote cell proliferation was more than 80 percent.The activity lasted a few months after being exposed to radioisotope.Conclusion　The extract prepared by heat-treating not only had high activity, but also had the unique method and good repeatability.This prepared extract as a kind of stable,reliable and remarkably promoting lymphocyte proliferation reaction had the value of development production on broad scale as well as in clinical practice.

Objective To assess the value of C-reactive protein(CRP) for the prognosis of acute pulmonary embolism (PE). Methods 56 acute pulmonary embolism patients, confirmed by spiral computed tomography (sCT), magnetic resonance imaging(MRI), or pulmonary angiography (CTPA). CRP as well as electrocardiogram (ECG), echocardiography(UCG), blood gas analysis, were taken after admission. Results Among these patients,24 cases with higher CRP(≥10 mg/L),were diagnosed as massive (50.0%), sub-massive PE(45.8%), mini-massive (4.2%) or died(25.0%). 24 cases (100.0%) showed right heart dysfunction and 24 cases showed pulmonary hy-pertension on UCG, right ventricular 20 (83.8%) on ECG, 22 cases (91.7%) showed hypoxemia. 8 cases (33.3%) had syncope,and 9 cases (37.5%) had cardiogenic shock. CRP was lower than 10 mg/L in 32 patients, among whom,3 cases (9.4%) were with massive PE,6 cases (18.8%) were with sub-massive PE,23(71.8%) were with small PE,1 cases(6.3%) died. 13 (40.6%) had right heart dysfunction and 16(50.6%) had pulmona-ry hypertension on UCG, right ventricular on ECG in 15 cases (46.9%), hypoxemia in 11 cases (34.4%), syncope in 2 cases (6.3%), cardiogenic shock in 4 cases (12.5%). The occurrence of massive PE, mortality (P<0.01) and sub-massive PE (P<0.05)were statistically different between the groups with higher CRP and the groups with lower CRP. The ratio of right heart dysfunction, pulmonary hypertension, right ventricular overloading, hypoxemia had statistical significance (P<0.01). There was statistical significance in the occurrence of syncope, cardiogenic shock (P<0.05). Conclusions CRP can be used as a parameter for PE, and can be used to stratify risk levels for severi-ty and assess the effectiveness of treatment.

Objective To study the value of CRP and HCY in each TCM type of T2DM with HUA and provide the basis for classification and treatment.Methods 103 cases of T2DM and HUA were divided into four Chinese medicine syndrome groups including deficinecy syndrome of wind-cold and damp-heat phlegm accumlatoin and blood stasis syndrome deficinecy syndrome of Qi and Yin deficinecy syndrome of Yin and yang.Results The differentiation of TCM symptom among four groups was uncorrelated with the level of CRP and Hcy.But the serum CRP and HCY levels increased slighly as compared with those of normal control group.Conclusion The elevation of CRP and Hcy is closely correlated with phlegm accumlation and blood stasis syndrome in patients of T2DM with HUA.As an inflammatoy biomarker,CRP and Hcy may be regard as two of the microcosmic indicators of toxin in Chinese medicine.They can be used as two important indexes of TCM syndrome differentiation of T2DM with HUA.

[ ABSTRACT] AIM: To study the autophagy of prostate cancer PC-3 cells induced by CD147 in vitro.ME-THODS:Themethod of amino acid starvation to induce autophagy was used.The expression of CD147 was detected by Western blotting.To study the functional effects of CD147 on autophagy in prostate cancer PC-3 cells, the down-regulation of CD147 expression was induced by the technique of RNAi.The conversion of autophagic marker protein LC3-I to LC3-II was determined by Western blotting.The cell death after starvation-induced autophagy was analyzed by trypan blue exclu-sion assay.RESULTS:The CD147 expression gradually increased in starvation-induced autophagy.The down-regulation of CD147 significantly increased the expression of autophagy-related protein LC3-II compared with control group.Mean-while, the cell death rates increased from (19.3 ±3.1)%and (22.3 ±3.5)%in control groups to (38.4 ±3.1)%in si-lencing the expression of CD147 in the PC-3 cells (P<0.05).CONCLUSION:CD147 inhibits starvation-induced auto-phgy and autophagy death in the prostate cancer PC-3 cells.

To investigate the effects of electro-acupuncture at Shuigou (DU26) on latency and amplitude of motor evoked potential (MEP) in rats after cerebral infarction.

Objectives To investigate the effect and mechanism of terbutaline sulfate on pulmonary fluid transport in pre-mature rats. Methods Pregnant rats were randomly divided into 5 groups (control group, premature group, low-dose terbutaline group, high-dose terbutaline group and dexamethasone group). Drugs were administered by gavage after rats were fertilized for 16 days and continued for 3 days. Premature rats were taken out from the 19 days pregnant rats, and mature rats were delivered on the due day. Lungs were collected, and the ratio of pulmonary wet weight to dry weight (W/D), Na+, K+-ATP ase activity and concentration of cyclic adenosine monophosphate (cAMP) were measured in lungs. Results The W/D rate, Na+,K+-ATPase acti-vity and cAMP concentration in lungs had significant difference among different groups (P<0.01). The W/D rate was highest in premature group and lowest in the control group. It was lower in the high-dose terbutaline group than in the low-dose terbutaline group and the dexamethasone group (P<0.05). The Na+,K+-ATPase activity was lowest in premature group and highest in high-dose terbutaline group. It was higher in dexamethasone group, low-dose terbutaline group, and high-dose terbutaline group than in premature group, and it was higher in high-dsoe terbutaline group than in low-dose terbutaline group and dexamethasone group (P<0.05). The cAMP levle was lowest in premature group and highest in high-dose terbutaline group. It was higher in dexamethasone group, low-dose terbutaline group, high-dose terbutaline group than in premature group, and it was higher in high-dose terbutaline group than in low-dose terbutaline group and dexamethasone group (P<0.05). Conclusions Terbutaline sulfate facilitates lung fluid transport in premature rats, leading to reduce the W/D rate in terbutaline-treated group. We speculate that this effect is related to the increased cAMP level and Na+, K+-ATPase activity in lung tissue.