Objective: To study the adjustment amplitude of blood pressure in patients with hypertensive intracerebral hemorrhage and the guiding significance in clinical practice in monitoring the changes of cerebral hemodynamics by transcranial Doppler (TCD). Methods: The blood pressures of sixty-four hospitalized patients with intracerebral hemorrhage were monitored dynamically within 10 hours after the onset. Among them, 38 patients had intracranial hematoma puncture and tube drainage within 24 hours of hospitalization (operation group), and 18 patients had conservative treatment (conservative group). Patients were monitored with TCD at the time of hospitalization on day 1, 4, 7, and 14. Another 8 unoperated patients (antihypertensive group) were monitored with TCD before and after antihypertensive treatment, and they were compared with the TCD parameters of 26 simple hypertensive patients (control group) before and after antihypertensive treatment. Results: There was no significant difference in TCD parameters in the control group before and after antihypertensive treatment, however, the blood flow velocity of bilateral middle cerebral arteries (MCA) decreased, and the pulsatility index (PI) increased after antihypertensive treatment in the antihypertensive group. The blood pressure in patients with intracerebral hemorrhage decreased gradually during the treatment, while the blood flow velocity of bilateral MCA decreased first, and then increased. The PI values of bilateral MCA increased first, and then decreased. The blood flow velocity in the conservative group decreased within the first week of the onset. It began to increase at week two and the changes of PI values were contrary to it. The blood flow velocity of MCA increased on day 7 and PI values decreased in the operation group, and there was significant difference between the 2 groups on day 14. The blood pressure decreased more significant in the operation group than that in the conservative group, and there were significant differences in systolic pressure between the 2 groups on day 14 (P

Background and purpose:About 80%patients with oral and maxillofacial malignant tumor are oral squamous cell carcinoma (OSCC). OSCC is one of the most common cancers in the world with less than 50%survival rate over 5 years. This experiment aimed to explore the effect of stanniocalcin 2 (STC2) on apoptosis, proliferation, migration and invasion of OSCC cell. Methods:RNA interference (RNAi) vector pLKO.1-shSTC2 was constructed and transfected into KB cells. Cell proliferation and cell apoptosis were then assessed by CCK8, APC Annexin V/7-AAD and lfow cytometry. Differences of migration and invasion between KB scr and KB STC2i were examined by cell scratch and transwell tests. Finally, this study detected the apoptosis-associated proteins and metastasis-associated proteins by Western blot. Results:STC2 down-regulation plasmid was constructed and transfected into KB cells. CCK8 prolifera-tion assay revealed that the STC2 down-regulation inhibited KB cells proliferation. By treating with cisplatin, this study found that STC2 silence could facilitate cell apoptosis signiifcantly. With the knock down of STC2 gene, the expressions of Bcl-2, Caveolin-1 andβ-catenin were decreased but the expression of bax was obviously increased. Conclusion:These data suggest that STC2 may be involved in the apoptosis, proliferation, migration and invasion of OSCC KB cells. Simultaneously, it can signiifcantly weaken the sensitivity of KB cells to chemotherapeutic drug cisplatin.

Purpose:We evaluated the sensitivity and specificity of p53 antibodies (p53-Ab) as a preoperative tumor marker and as a prognosis marker in colorectal cancer patients and we also investigated whether p53-Ab production is dependent on p53 protein overexpression in tumor tissue.Methods:Serum samples from 178 colorectal cancer patients and 40 healthy controls were tested for the presence of p53-Ab by using an ELISA kit. Expression of p53 protein in tumors was determined using mouse anti-human p53-Ab.Results:Serum p53-Ab was detected in 18.54% of patients while all controls were negative. A strong correlation between p53-Ab production and p53 protein expression was observed: 69.7%of p53-Ab positive cases had tumors positive for p53 vs 30.3%of p53-Ab negative cases. There was also a significant predominance of p53-Ab positive cases in Dukes ’ stages B (16/71,22.54%), C (12/55,21.82%)and D(5/30,16.67%)over stage A. No difference in patients’ age, sex and localization was found between the p53-Ab positive and negative groups. Recurrence of cancer did not differ between p53-Ab positive and negative patients after a median follow-up of 13.5 months and also median survival time did not vary significantly.Conclusions:There was a significant correlation between p53-Ab production in serum and p53 protein expression in tumor tissue. Because of their low sensitivity (18.54%) p53-Ab are not suitable as a preoperative tumor marker. However, their high specificity (100%) and their potential for early diagnosis of a tumor relapse makes them valuable for postoperative monitoring during follow-up in p53- Ab positive patients.[

Objective To improve the theoretical basis for the typical hemodynamic changes in subclavian steal syndrome (SSS) patients underwent transcranial doppler (TCD) by detailed hemodynamic analysis.Methods Patients with SSS were examined by transcranial doppler and some patients performed tourniquet test.Flow spectrum,flow velocity and pulsatility index (PI) of vertebral artery,basilar artery and middle cerebral artery (MCA) were recorded.Results The flow spectrum of steal side vertebral artery showed that there were 14 cases with systolic notch,18 cases with systolic reverse flow and 3 cases with flow completely reversed.The flow spectrum showed high resistance spectrum in vertebral artery and basilar artery and ipsilateral basilar artery vortex or turbulence in 32 cases.The PI value was higher in contralateral vertebral artery and basilar artery than in anterior circulation (q =9.56,9.98,both P ＜ 0.01),and there was no difference between contralateral vertebral artery and basilar artery (q=0.416,P＞0.05).The tourniquet test were positive in all 22 patients.Conclusions TCD is a convenient,fast,accurate and non-invasive method for hemodynamic examination,which is the first choice for the diagnosis of SSS.

AIM:To identify and analyze tyrosine-phosphorylated proteins regulated by protein tyrosine phos-phatase-like A domain containing protein 1 ( PTPLAD1) in colon cancer cells by phosphoproteomics.METHODS: The expression of PTPLAD1 in colon cancer cell line HCT-116 was knocked down by small interfering RNAs, and the differenti-al expression of tyrosine-phosphorylated proteins in response to the konckdown of PTPLAD1 in HCT-116 cells was identified by stable isotope labeling with amino acid in cell culture ( SILAC) , coupled with the tyrosine phosphorylation antibody im-munoprecipitation and LC-MS/MS analysis.The Ingenuity Pathway Analysis ( IPA) software was employed for bioinformat-ics analysis on the differentially-expressed proteins.RESULTS:A total of 20 differentially-expressed tyrosine-phosphoryla-ted proteins were identified by MS, including 8 markedly up-regulated and 10 evidently down-regulated proteins.IPA soft-ware suggested that these proteins were mainly associated with the disease of cancer, tissue development and function, and cell death and survival.CONCLUSION:We successfully identified PTPLAD1-regulated differentially-expressed tyrosine-phosphorylated proteins in colon cancer cell line HCT-116.Our analysis suggests that PTPLAD1-regulated proteins in colon cancer are closely correlated with colon cancer.

Adult ; Hematopoietic Stem Cell Transplantation ; methods ; Humans ; Male ; Primary Myelofibrosis ; surgery ; Transplantation Conditioning ; methods

Cor pulmonale is a common disease. The intervention varies and the effectiveness remains unsatisfactory. The vasodilators have been studied intensively in recent years and their application in treating the disease has been developing quickly. In this paper, the advances of research on the vasodilators in treatment of pulmonary hypertension in the last 5 years is reviewed, the advantages and disadvantages of the drugs presented, and a discussion on the trend of treatment of pulmonary hypertension made.

Objective To observe the effect of montelukast sodium and ambroxol oral solution in treatment respiratory symptoms caused by mycoplasma infection in children.Methods 147 children who were infected by mycoplasma pneumonia with lower respiratory tracts symptom were randomly divided into A,B,C three groups:group A was control group,group B was treated by montelukast sodium,and group C was treated by montelukast sodium and ambroxol oral solution.Coughing,symptomatic relief of pant were observed in each group.Results After 7 days treatment,the total effective rate of three groups was A group 79.5％,B group 95.7％,C group 96.4％.Compared with group A,the coughing and panting time in group B and group C were significantly shorter(x2 =5.61,9.54,all P ＜0.05),The rate of coughing and panting complete remission in group C was higher compared with group B (x2 =5.39,P ＜ 0.05),but there was no statistificantly significant difference in the total efficency between the two groups.Conclusion Montelukast sodium and ambroxol oral solution can effectively improve the symptoms of respiratory tract infection caused by mycoplasma pneumoniae.

Objective Measure the epicondyles of the distal femur on magnetic resonance image(MRI), in order to locate presicely the surgical transepicondylar axis(STEA) in total knee arthroplasty(TKA) and to provide theoretical basis for the designing of the size of the femoral component. Methods Totally 78 normal knees of Chinese individuals were studied. The images of coronal, sagittal and traverse sections of the knee were obtained by magnetic resonance image. Measurement included the width of the STEA, distance between the epicondyles and the joint line, anterior posterior width of the medial and lateral epicondyles, posterior condyle angle. Results The width of the STEA averaged(79.55±4.90)mm in males, and femles(71.18±4.22)mm. The distance from the epicondyles to the joint line was correlated with the width of the STEA, so was the anterior posterior width of epicondyles. PCA averaged(4.22±2.07)°. Conclusion The size of the epicondyles in Chinese is significantly smaller than that of the Westerns. The ratio between anterior posterior width of the medial epicondyle and the width of the STEA is 0.84, and is 0.87 between anterior posterior width of the lateral epicondyle and the width of the STEA. The distance from the epicondyles to the joint line is helpful to locate the STEA. The reliability is poor to locate the STEA by touch or PCL.

Objective:To discuss the therapeutic effect of pharmaceutical care for old patients with chronic obstructive pulmonary disease ( COPD) . Methods:Totally 192 patients diagnosed as COPD were randomly divided into 2 groups according to a random num-ber table, 100 cases in the experimental group and 92 cases in the control group. The control group was treated with the conditional therapy, and the experimental group was treated with pharmaceutical care additionally. The studieds on COPD assessment test ( CAT) , modified British medical research council ( mMRC) and the value of pulmonary function index ( FEV1% index) were carried out and compared between the two groups. Results:There was no statistically significant difference in the CAT score, mMRC classification and FEV1% index between the two groups on admission (P>0. 05), however, after the treatment, the CAT score and mMRC classifica-tion were decreased and FEV1% index was increased in the two groups, and there was statistical significance between them ( P <0. 05). The CAT score and mMRC classification in the experimental group were lower and FEV1% index was higher than that in the control group(P<0.05). Compared with the medication errors(n=13, 14.13%) and incidence of adverse drug reactions(n=5, 5. 43%) in the control group, the medication errors(n=2, 2. 0%) and incidence of adverse drug reactions (0%) in the experimental group were significantly decreased. Conclusion:Clinical pharmacists provide effectively pharmaceutical care, which can significantly improve the clinical therapeutic efficacy in old patients with COPD.