GERL in fetal hepatocytes of 12, 15, 17, 19 and 21 days Wistar rat fetuses was investigated by means of transmission electron microscopy and ultracytochemistry. The ultrastructural study showed that: on the day 12 fetuses, GERL of fetal hepatoeytes was consisted of rigid lamellae and short tubules. Rigid lamellae were characterized by the relatively rigid, thin tubules and located at the maturation face of Golgi apparatus. On the day 15 and 19 fetuses, GERL was composed of tubulevacuole-like rigid lamellae, some vesicles, short or thick tubules and round dence bodies. GERL was often found tobe contiuned with lysosomes, including coated vesicle, primary and secondary lysosomes. On the day 17 and 21 fetuses, when the structure of fetal hepatocytes was at the stage of differentiation and renewal, GERL degenerated and could notbe identified.Acid phosphatase ultraeytochemieal results showed that the enzyme reaction product was seen in all parts of GERL and lysosomes, however, no portion of the Golgi stack showed AcP activity. AcP reaction product could also demons trated in the network form and degenerated form of GERL.As mentioned above, our study suggested: (1) there was indeed GERL in fetal hepatocytes of rat fetuses; (2) the formation of GERL was related to the differentiation of fetal hepatocytes as well as the formation and function of lysosomes; (3) according to the unique ultrastructure, enzymological and functional features, it be a preference to the view that the GERL might be a distinct cytoplasmic organelle.

Anticonvulsants ; pharmacology ; therapeutic use ; Epilepsy ; drug therapy ; genetics ; Humans ; Pharmacogenetics

Objective To investigate the effects of ligustilide on the proliferation of human lung adenocarcinoma A549 cells and its mechanism. Methods CCK-8 method was used to detect the effects of ligustilide on activity of A549 cells. Apoptosis rate was measured by TUNEL. Nuclear morphological changes of A549 cells were observed by fluorescence microscope after staining by Hoechst 33258. ELISA was used to detect the VEGF levels after incubation with ligustilide. Western blot was used to detect the expression of NF-κB, Bcl-2, Bax and the protein expression of phosphorylation of JNK. Results A549 cell activity was significantly inhibited after incubated with 15, 30, 60, 120, 180μmol/L ligustilide for 12, 24, 48 h (P<0.05, P<0.01, P<0.001) in a dose and time-dependent manner (P<0.05, P<0.01);Apoptosis rate increased by 30, 60, 120 μmol/L ligustilide for 12, 24, 48 h in a dose and time-dependent manner;A549 cells treated with ligustilide for 48 h appeared the apoptosis forms, including nuclear shrinkage, beating, strong blue fluorescence staining, and the chromatin divided producing apoptotic body;30, 60, 120μmol/L ligustilide increased the expression of p65 subunit of NF-κB protein in the nuclei and the phosphorylation levels of JNK protein, reduce the expression of suppression apoptosis protein Bcl-2, and raised the expression of pro-apoptotic protein Bax (P<0.05, P<0.01, P<0.001). Conclusion By inhibiting the expression of VEGF, ligustilide can inhibit the formation of tumor angiogenesis. By regulating the expression of NF-κB and the phosphorylation levels of JNK protein and reducing the ratio of Bcl-2/Bax, ligustilide can promote tumor cell apoptosis.

Objective To investigate the proliferation inhibitory effect and mechanism of Ginkgo Biloba extract (EGB) on non-small cell lung cancer (NSCLC) PC-9 cells. Methods Concentrations of EGB were set as 70 mg/L, 105 mg/L, 140 mg/L, 210 mg/L, and 280 mg/L, and were used to culture PC-9 cells in vitro for 24 h, 48 h, and 72 h. Tetramethylazo thiazole blue staining (MTT) method was applied to detect cell inhibition rate. Hoechst33258 fluorescent staining was employed to observe the nucleus. SOD activity and MDA content were detected by ELISA. The protein expressions of Caspase-3, Caspase-9, Cyt-C, and AIF were detected by Western blot. Results After incubated for 24 h, 48 h, and 72 h, EGB inhibited the proliferation of PC-9 cells, IC50 was 195.45 mg/L, 179.63 mg/L, 142.23 mg/L, respectively). 105 mg/L, 140 mg/L, 210 mg/L EGB could induce apoptosis of PC-9 cells, cause the nucleus pycnosis, produce apoptotic bodies, improve SOD activity and decrease MDA content (P<0.05, P<0.01, P<0.001). Western blot results showed that, compared with the control group, EGB can obviously increased the protein expressions of Caspase-3, Caspase-9, Cyt-C, and AIF, with statistical significance (P<0.05, P<0.01, P<0.001). Conclusion EGB can effectively restrain proliferation of non-small cell lung cancer PC-9 cells, the mechanism may be realized by inducing PC-9 cell apoptosis through pathway of mitochondria apoptosis, reducing oxidation, and clearing free radicals.

Increased evidences indicate that resistin is a new hormone secreted from adipose tissue, it is reported to be an important signal molecule linking between obesity,insulin resistance and type 2 diabetes. Many factors can affect the gene expression of resistin. However, the detailed function of resistin still remains mysterious and much work needs to be undertaken. [

Objective:To construct a recombinant lentivirus plasmid of RNA interference targeting (MSLN) gene and to observe its effect on MSLN expression in human ovarian cancer cell line OVCAR-3 and its effect on cell proliferation. Methods: According to the Genbank information of MSLN, four RNA interfering sequences and a negative sequence were designed and inserted into plasmid pRNAT-U6.2/Lenti and 5 kinds of plasmids were packaged: LV-MSLN-negative,LV-MSLN-shRNA1, LV-MSLN-shRNA2, LV-MSLN-shRNA3, and LV-MSLN-shRNA4; and they were used to transfect OVCAR-3 cells. Western blotting and indirect immunofluorescence were then used to investigate the interfering efficiency. The plasmid with high interfering efficiency was packaged. The cell proliferation test and clone-forming test was used to assess the changes in cell proliferation. Results: DNA sequencing showed that the sequences of 5 recombinant lentivirus plasmids were correct. Lentivirus packaging was successfully done. Western blotting analysis confirmed that LV-MSLN-shRNA4 had the highest interfering efficiency (90%). MSLN specifically bound to cytomembrane of OVCAR-3 cells. Expression of MSLN in the interfered cells (OVC-shRNA) was weaker than that in the control cells (OVC-neg,OVC). OVC-shRNA cells([11.2?1.3]?105) grew slowly compared to OVC-neg cells([20.5?2.5]?105) and OVC cells([21.9?2.3]?105) (P

Objective To investigate the function of mesothelin by analyzing the location,the expressions and the adhesive ability of mesothelin and CA125 in human epithelial ovarian cancer.Methods Mesothelin and CA125 protein expressions and locations in human epithelial ovarian cancer tissues and cell lines were determined by indirect immunofluorescence double maker and Western blot.Cell adhesive ability was detected by EDTA-induced cell detachment.Results Mesothelin and CA125 were specifically located in cytomembrane of human epithelial ovarian cancer cells.Expressions of mesothelin in the SKOV-3 and 3AO cells were weaker than those in the OVCAR-3.SKOV-3 and 3AO cells adhered less effectively to plastic substance and matrigel than OVCAR-3(P

Objective To investigate the effect of protection motivation intervention on patients with diabetic cataract after implantable collamer lens implantation. Methods A total of 118 diabetic cataract patients were divided into 2 groups (59 cases each) by random digits table method. The control group received routine ophthalmic nursing and the observation group was given protection motivation intervention based on the routine nursing. The change of the plasma glucose, postoperative visual acuity and the quality of life were evaluated. Results The blood glucose on the morning of the operation day and one day after operation in the observation group was (8.27±1.25) mmol/L, (9.52±2.44) mmol/L, which was significantly lower than that of the control group, (10.49 ± 1.48) mmol/L, (12.77 ± 3.36) mmol/L, the difference between the groups was statistically significant (t=-5.392,-6.755, P<0.01). After 1 month and 3 months, the vision of the observation group was 0.64±0.22, 0.67±0.30, which was higher than that of the control group 0.56± 0.18, 0.58 ± 0.20, in which the postoperative blindness or low vision rate was 3.95%(3 eyes) in the observation group, and 13.51%(10 eyes) in the control group(t/χ2=2.492, 2.209, 4.013,P<0.05). The quality of life in the observation group was 7.70±1.13 and 5.22±1.48 in the control group, there was statistical significance between the two groups(t=3.660, P < 0.05). Conclusions Application of protection motivation intervention on diabetic cataract patients can improve their postoperative vision and quality of life.

Objective: To develop the Chinese version of the Neuropsychiatric Inventory Questionnaire (NPI-Q) and evaluate its reliability and validity. Methods: Three investigators assessed 10 dementia cases together with the NPI-Q to evaluate the inter-rater reliability. Eighty-six dementia cases and 30 healthy controls were assessed with the Behavioral Pathology in Alzheimer's Disease (BEHAVE-AD), the Brief Psychiatric Rating Scale (BPRS) and NPI-Q to evaluate the criterion validity. A subgroup of 30 dementia cases was tested with the NPI-Q again 24 h later to evaluate the test-retest reliability. Results: (1) Internal consistency. Cronbach's a coefficient of the severity subscale was 0.589, mean inter-item correlation coefficient of the severity subscale was 0.107. Cronbach's α coeffi-cient of the distress subscale was 0.612, and the mean inter-item correlation coefficient of the distress subscale was 0.114. (2) Inter-rater reliability. Intraclass correlation coefficient (ICC) of the severity subscale was 0.97, and ICC of the distress subscale was 0.94. (3) Test-retest reliability. The test-retest correlation coefficient of the severi-ty subscale was 0.89, and the test-retest correlation coefficient of the distress subscale was 0.86. (4) Criterion va-lidity. The severity subscale total score was correlated significantly with BEHAVE-AD (r=0.70, P <0.001) and BPRS(r=0.40, P<0.001) total score; the distress subscale total score was correlated significantly with the global ratings in part 2 of BEHAVE-AD (r=0.76, P<0.001) . (5) The severity subscale total score in demen-tia group was significantly higher than that in control group (median: 10 vs.1.5, P <0.001). The distress sub-scale total score in dementia group was significantly higher than that in control group (median: 10 vs 0; P< 0.001) . (6) Exploratory factor analysis identified 4 common factors, including psychosis, abnormal behavior,discontrol and affective symptoms, which explained 58.3% of total variance. All of the items loaded were between 0.596 and 0.803 on their conrresponding factor. Conclusion: The reliability and validity of the Chinese version of NPI-Q meet the psychometrics properties, and deserves to conduct further study in larger samples.

Objective To study the relation between the TCM constitution and the lung function of asthmatic bronchitis children to lay a foundation to apply the TCM constitution theory in preventing and treating children asthmatic diseases.Methods Following the Zhu's method,120 asthmatic bronchitis children were divided into normal constitution,phlegm-damp constitution,qi deficiency constitution,internal heat constitution,and both qi and yin deficiency constitution.Based on the Su's method,the cases were classified into balance constitution,lung-spleen Ⅰ constitution,lung-spleen Ⅱ constitution,spleen-kidney Ⅰ constitution,and spleen-kidney Ⅱ constitution.Their lung function was tested and the TPTEF /Te and VPTEF/Ve of each constitution were analyzed.Results Of the 120 cases,37 ones (30.8%) were qi deficiency constitution;33 ones (27.5%) were phlegm-damp constitution;and 60 ones (50.0%) were lung-spleen Ⅱ constitution.Compared with those with normal constitution,the cases with the phlegm-damp constitution,qi deficiency constitution,internal heat constitution,and both qi and yin deficiency constitution had significantly lowered TPTEF/Te and VPTEF/Ve (P