Objective: To investigate the clinical efficacy of prednisone combined with aminomethylbenzoic acid in the treatment of patients with irritability purpura and its effects on inflammatory factors and coagulation function.Methods: Totally 98 cases of patients with irritability purpura were randomly divided into the observation group (n=49) and the control group (n=49).The control group was treated with prednisone, while the observation group was given aminomethylbenzoic acid injection on the basis of the control group.Both groups were treated for 4 weeks.The efficacy of the two groups was compared, and the changes of serum inflammatory factors and coagulation function before and after the treatment, the disappearance time of stomachache, purpura and arthralgia and adverse drug reactions were also observed and compared.Results: The total effective rate of the observation group (93.88%) was significantly higher than that of the control group (73.47%, P<0.05).After the treatment, the serum level of IL-12 increased, the IL-15 and IL-23 levels decrease, and the D-D dimer and Fib levels decreased in both groups;the serum level of IL-12 in the observation group was higher than that in the control group, and the IL-15 and IL-23 levels were lower than those in the control group (P<0.05);the D-D dimer and Fib levels in the observation group after the treatment were lower than those in the control group (P<0.05).The disappearance time of abdominal pain, purpura and joint pain in the observation group was significantly shorter than that in the control group (P<0.05).ECG, blood and urine routine, liver and kidney function were normal in both groups without serious adverse reactions.Conclusion: Prednisone combined with aminomethylbenzoic acid in the treatment of irritability purpura shows striking effects without obvious adverse reactions, and the actions may be related to reducing the inflammatory reaction and improving coagulation function, which is better than prednisone used alone.

Objective:To investigate the relationship of micro-inflammation,cellular and humoral immune function and kidney disease of patients with diabetic nephropathy. Methods:124 cases of DN were randomly divided into normal albuminuria group ( NA, n=35),microalbuminuria group (MA,n=45) and clinical albuminuria groups(CP,n=44) based on 24 h urinary albumin excretion rate ( UAER) and the healthy subjects were selected as the control group ( n=35 ) . The levels of C-reactive protein ( CRP ) were measured with radioimmunoassay,the levels of interleukin-6(IL-6),tumor necrosis factor-α(TNF-α) and humoral immunity(IgG enzyme-linked immunosorbent assay,IgA,IgM) were measured with ELISA. The levels of immune response ( CD4+Th17,Th17/Treg, CD4+ CD25+ Treg) were measured with flow cytometry cellular. The levels of creatinine ( Scr) ,cystatin C ( CYSC) and UAER of each groups were analyzed with fully automated biochemistry. Results:The levels of CRP,IL-6,TNF-α,Scr,CYSC,UAER of NA group,MA group,CP groups were higher than control groups(P<0. 05),the levels of CRP,IL-6,TNF-α,Scr,CYSC,UAER of CP groups were higher than NA,MA group (P<0. 05). The levels of CD4+CD25+ Treg,IgG were lower than the control group (P<0. 05),while the levels of CD4+ CD25+ Treg,IgG of CP groups were higher than NA group,MA group (P<0. 05). The levels of IgA,IgM,Th17/Treg, CD4+ Th17 of NA group,MA group,CP groups were higher than control groups(P<0. 05). The levels of CRP,IL-6,TNF-α were positively correlated with Scr, CYSC, UAER ( P<0. 05 ) and were negatively correlated with Th17/Treg, CD4+CD25+ Treg, IgG ( P<0. 05). Conclusion:DN patients with micro inflammatory status and immune function disorder,through the control or elimination of pro-inflammatory factors,improve the immune function of DN patients to delay the occurrence and progress of DN patients has important significance.

Accidents, Occupational ; statistics & numerical data ; China ; Humans

Bone marrow mesenchymal cells (BMSCs) are regarded as donor cells in cell transplantation therapies for spinal cord injury (SCI) for they have the ability of favourable proliferation and multi-directional differentiation, and are easily isolated and culturd and have less immunological reaction. It has been confirmed that subarachnoid space injection is the most ideal delivery technique of BMSCs. Bone marrow mesenchymal stem cells transplantation is safe and its reconditioning role is certain for SCI in early clinical application. The mechanism of BMSCs promoting functional recovery after SCI is probably concerned with vicarious function, nerve trophism, immunosuppression and promoting axonal regeneration by BMSCs.
Animals ; Bone Marrow Cells ; cytology ; Humans ; Mesenchymal Stem Cell Transplantation ; methods ; Mesenchymal Stromal Cells ; cytology ; Spinal Cord Injuries ; surgery

Objective To investigate the effects of DDT (dichloro-diphenyl-trichloroethane) residuals in the surrounding environment of a pesticide plant on the health of women and children. Methods 50 pairs of children aged 6-10 years and their mothers were investigated during Jan-Jul, 2005. The venous blood samples were collected, the levels of DDT and its 4 metabolites (p,p’-DDE, p,p’-DDT, p,p’-DDD and o,p’-DDT) were determined by GC-ECD and the biochemical indexes of blood were assayed. Each participant completed a questionnaire for information on health history and lifestyle and underwent a prenatal health examination. Results The blood lipid levels were abnormal in 18 percent of mothers. The level of blood total DDT in mothers and children was 8.42 and 11.98 ng/ml respectively. o,p’-DDT and p,p’-DDD were not detected. The level of p,p’-DDE and total DDT in children’s blood was higher than that in the women’s. Conclusion DDT has been accumulated in some degree in the blood of women and children in the area.

Cochlear Implantation ; Cochlear Implants ; Humans ; Tinnitus

Objective To explore the interactions between human leukocyte antigen ( HLA)-DRB1 gene polymorphism and environmental risk factors in gestational diabetes mellitus ( GDM ) pathogenesis.Methods Pregnant women who had prenatal cares in Obstetric Department , West China Second Hospital of Sichuan University were recruited from January 1st to December 31st in 2011.A prospective cohort study was conducted in the women who had a glucose challenge test ( GCT) or 75 g oral glucose tolerance test ( OGTT) during 24-28 gestational weeks.A total of 104 women diagnosed with GDM were randomly included in GDM group while another 103 normal women fell into the control group.The HLA-DRB1 polymorphism was detected by Polymerase Chain Reaction -Sequence Specific Primers ( PCR-SSP) method in both groups.The interactions between HLA-DRB1 polymorphism and environmental risk factors were analyzed based on the simple-case-study method.Results ( 1 ) There were 712 pregnant women with complete perinatal information during January 1st to December 31st, 2011, among whom 175 (24.6%) women were diagnosed with GDM.A logistic regression analysis showed that advanced maternal age (OR=1.081, 95%CI:1.027-1.138), imbalanced diet (OR=3.329, 95%CI:2.167 -5.116), high body mass index (BMI≥24.0 kg/m2) before pregnancy (OR=1.095, 95%CI:1.008 -1.190), HBsAg carrier status (OR=3.173, 95%CI:1.387-7.260) and family history of diabetes mellitus (DM) (OR=1.798, 95%CI:1.063 -3.041) were risk factors of GDM.(2) There were 49 HLA-DRB1 genotypes and 51 HLA-DRB1 genotypes in GDM group and the control group , respectively.We further compared the genotypes that occurred in over 3 cases in either group and found that HLA-DRB1*12,16 was only detected in 5 cases (5/103, 4.9%) in control group,and the difference was significant between the two groups (P=0.029).HLA-DRB1*11,16 and HLA-DRB1*09,09 were only detected in 4 cases (3.8%,4/104) and 5 cases (4.8%, 5/104) in GDM group respectively , but without significant differences between the two groups ( P >0.05 ).No significant difference was found in other genotype frequencies between the two groups ( P>0.05 ).( 3 ) Thirteen types of HLA-DRB1 allele were detected but no significant differences were observed in their frequencies between two groups ( P>0.05).(4) A positive interaction was detected between HLA-DRB1*07 polymorphism and advanced maternal ages (OR=5.952, 95%CI:1.314-26.970, P=0.022), while no interaction was found between HLA-DRB polymorphisms to other risk factors such as imbalanced diet , high body mass index ( BMI≥24.0 kg/m2 ) , HBsAg carrier status or DM family history.Conclusions Advanced maternal age, unbalanced diet, high body mass index (BMI≥24.0 kg/m2), HBsAg carrier status and DM family history are environmental risk factors of GDM in Chengdu.While HLA-DRB1*12,16 genotype may be a protective genotype for GDM.There is a positive interaction between HLA-DRB1*07 polymorphism and advanced maternal age which may play a critic role in GDM development.

Antifungal Agents ; therapeutic use ; Child, Preschool ; Cryptococcosis ; drug therapy ; Fluconazole ; therapeutic use ; Humans ; Male

Objective:To detect the expression of cyclin B 1 in human ameloblastoma(AB). Methods:Expression of cyclin B1 was detected in 73 cases of AB (32 primary, 33 recurrent and 8 malignant cases), 19 of odontogenic keratocyst (OKC) and 7 of normal oral mucosa by streptovidin-biotin method. Results:The ratio of positive nuclear expression of cyclin B 1 in normal oral mucosa, OKC and AB was 28.6%(2/7), 36.8%(7/19) and 63.0%(46/73) respectively (P

Objective To investigate the effects of fluoride on the cell viability, cell cycle, apoptosis, and expression levels of NF-?B in primary cultured rat hippocampal neurons. Methods The rate of cell survivor, distribution of cell cycle, the percentage of apoptotic cells and the mRNA expression level of NF-?B were respectively measured by methyl thiazolyl tetrazolium (MTT), flow cytometry (FCM), and reverse transcriptase polymerase chain reaction (RT-PCR) after the hippocampal neurons were incubated with 20, 40, and 80 ?g/ml sodium fluoride for 24 hours in vitro. Results Compared with the control group, the rate of cell survivor were significantly decreased in 80 ?g/ml fluoride-treated group (P