Objective To investigate the effect of different frequencies of pudendal electrical nerve stimulation on female stress urinary incontinence.Methods 60 SUI female patients from Shanghai Research Institute of Acupuncture and Meridian affiliated Yue Yang Hospital were enrolled in a three-times-per-week treatment group (30 paients) and a twice-per-week treatment group (30 patients).Both groups were treated by pudendal nerve electrical stimulation for twelve times.The change of therapeutic effect,symptoms index were observed in both groups before and after the treatment.Results ① Comparison of clinical efficacy:The therapeutic rate of three-times-per-week treatment group was 63.3％ (19/30),the total effective rate was 90.0％ (27/30); the therapeutic rate of twice-per-week treatment group was 36.7％ (11/30),the total effective rate was 76.7％ (23/30).The therapeutic rate showed significant differences between the two groups (x2=4.267,P＜ 0.05); The total effective rate showed no differences between the two groups (x2 =l.920,P＞0.05).② Comparison of clinical symptom score and life quality score:the post-treatment clinical symptom scores of the both groups improved significantly compared with the pre-treatment (tthee-times-prt-week=7.972,ttwice-per-week=9.520,both P＜0.01).Clinical symptom score of three-times-per-week treatment group improved better than twice-per-week treatment group (P＜0.01).Conclusion Three-times-per-week of pudendal nerve electrical stimulation has a better effect on treating female SUI patients.

Adult ; Aged ; Female ; Heart Neoplasms ; pathology ; Heart Valves ; pathology ; Humans ; Lymphangioma ; pathology ; Middle Aged

This paper summarizes the recent 6～7 year advances in the research of pharmacological effects of polyactin including mainly six aspects: ①Effects of polyactin on functions of macrophages in vitro ; ② Mechanism enhancive effect ofpolyactin on NK cell activity; ③Effects of polyactin on the human lymphocyte immunofunction; ④Effects of polyactin on the proliferation and cytotoxicity of LAK; ⑤Enhances in specific antitumor activity in mice in vivo ; ⑥Effects of polyactin on erythrocyte deformability and immunofunction in Rabbit and Rat.

OBJECTIVETo observe the effect on female stress urinary incontinence (SUI) treated with the pudendal nerve stimulation of electroacupuncture therapy at different frequencies.

METHODSSixty patients were divided into a group treated three times every week (group A) and a group treated twice every week (group B), 30 cases in each one. The pudendal nerve stimulation of electroacupuncture therapy was adopted at Disixue (four acupoints on sacral region), 60 min in each time in the two groups, treated three times a week or twice a week respectively. SUI severity and the score of life quality were observed before and after 12 treatments separately. The efficacy was evaluated in the two groups.

RESULTSAt the end of treatment, SUI severity was relieved and the score of life quality was improved in the two groups (all P<0.01). The results in group A were superior to group B [0 (0-4.3) vs 4 (0-5), P<0.01]. At the end of treatment, the total effective rate in group A was 90.0% (27/30) and was 76.7% (23/30) in group B; the efficacy in group A was better than that in group B (P<0.05).

CONCLUSIONThe pudendal nerve stimulation of electroacupuncture therapy achieves the definite efficacy on female SUI and the results in the treatment of three times a week are superior to those in the treatment of twice a week.

Acupuncture Points ; Acupuncture Therapy ; Adult ; Aged ; Electroacupuncture ; Female ; Humans ; Middle Aged ; Quality of Life ; Treatment Outcome ; Urinary Incontinence, Stress ; psychology ; therapy

Animals ; Aorta ; metabolism ; pathology ; Atherosclerosis ; etiology ; metabolism ; pathology ; Cholesterol, Dietary ; Male ; Matrix Metalloproteinase 2 ; metabolism ; PPAR gamma ; agonists ; Rabbits ; Random Allocation

Hepatitis B is a serious hazard to human health.This paper describes the current clinical use of several nucleoside antiviral drugs combination,and introduces the candidate drugs which are able to effectively inhibit the hepatitis B virus replication in vivo and in vitro in two years,which provides reference for the research and development of new anti-HBV drugs.

Phosphatidylserine (PS) is a phospholipid that is abundant in eukaryotic plasma membranes,has crucial biological functions.Under cell apoptosis, cells can not generate enough ATP for energy and the concentration of cytoplasmic Ca 2 +increases, resulting in PS eversion.Apoptosis and the clearance of apoptotic cells are essential processes in animal development and homeostasis.For apoptotic cells to be cleared, they must display aneat me signal, most likely PS exposure, which prompts phagocytes to engulf the cells.PS is exposed by the action of scramblase on the cell's surface in biological processes such as apoptosis and platelet activation.Once exposed to the cell surface, PS acts as an eat me signal on dead cells, and creates a scaffold for blood-clotting factors on activated platelets.The molecular identities of the flippase and scramblase that work at plasma membranes have long eluded researchers.Indeed, their identity as well as the mechanism of the PS exposure to the cell surface has only recently been revealed.We describe how PS is exposed in activated platelets and in apoptotic cells, and discuss the clearance of apoptotic cells.

In recent years, with the rapid development of antitumor targeted drug therapy, the preparation of antitumor liposomes and its targeting research have attracted attention of scholars. Anti-tumor targeted drug treatment requires the drug to reach the tumor site has a relatively high concentration and longer retention time, the tumor cells have a strong cytotoxic activity, while normal cells no significant side effects.The drug has an in vivo distribution and specificity for the target fine action.The preparation methods of liposomes include film dispersion method,reverse evaporation method,ethanol injection method,supercritical reverse phase evaporation method and freeze-drying method. Antitumor targeting liposomes can be divided into active targeting liposomes and passive targeting liposomes. Targeted liposomes can specifically target tumor cells by recognizing specific targets in the tumor tissue,thus enriching tumor cells and killing tumor cells. In this paper,the preparation of anti-tumor liposomes and the progress of its targeting,for future study and application of liposomes provide a reference.

Adjuvants have been used as critical components of vaccine.They were used to stabilize the antigens and potentiate the immune responses.Dose sparing is also of interests in recent years to be applied in potential pandemic situations or to lower the costs of vaccines.With the emerging nanotechnology in many aspects including the use in biomedical applications,the application of nanomaterials as vaccine adjuvants also attracted a lot of attentions in recent years.With favorable biological properties,such as well-defined and well-formed nanoparticles,biocompatibility,biodegradability and ability to induce humoral immunity and cellular immunity simultaneously,calcium phosphate nanoparticles (CaP) have the potentials to be developed as an immune potentiator to be used as vaccine adjuvants.Here,we reviewed the basic properties of CaP and their applications as vaccine adjuvants with antigens of different modalities such as inactivated virus,protein and naked DNAs.The mechanism of the adjuvanting effects is briefly described.Further the development of CaP as vaccine adjuvants with some designed surface modification could lead to next generation vaccine adjuvants with improved immune potentiating properties and safety profiles.

Objective To assess the changes of left ventricular function after off-pump coronary artery bypass (OPCAB) using Tei index. Methods Fourty-six patients of coronary artery disease, who had accepted OPCAB, were enrolled. Left ventricular ejection fraction(LVEF), E/A ratio at mitrial orifice(E/A), E deceleration time(EDT), isovolumic relaxation time(IVRT), ratio of systolic wave to diastolic wave of pulmonary vein flow(S/D) and Tei index were measured by transthoracic echocardiography before, immediate, 1 week, 1 month, 3 months and 6 months after OPCAB. Results Tei index decreased gradually as time went on after OPCAB and the differences were significant between different times(P