A high dose E. Coli endotoxin was infused into unanesthetized sheep withchronic lung lymph fistula. The contents of substance P in plasma, lung lymph, lungparenchyma and also TXB_2 and 6-keto-PGF_1? were measured. The results showed that 120min after infusion substance P increased in plasma and lymph, but its content in paren-chyma decreased at 360 min after infusion. At 30 min after infusion, TXB_2 and 6-keto-PGF_1? levels in plasma showed a marked increase, but their peak values in lung lymphwere shown at 60 min after infusion, and the levels of TXB_2 were much higher than thatof 6--keto--PGF_1?

To improve and optimize marine antimicrobial peptide R-1 production by a newly isolated Brevibacillus laterosporus Lh-1, Plackett-Burman (PB) design and response surface methodology (RSM) using central composite design was adopted in culture conditions. MINITAB 15.0 was used for planning the experiments, data analysis, contour diagrams and response optimizations. In this study, PB design was undertaken to evaluate the effects of the fifteen factors. By the statistical regression analysis, the significant factors affecting the novel antimicrobial peptide R-1 in submerged fermentation by Br. laterosporus Lh-1 were determined as follows: glucose, peptone and CaCl2. Then a RSM was used to optimize the above critical internal factors, and the optical concentration of the variables were deter-mined as: 15.72 g/L glucose, 6.01 g/L peptone and 3.29 g/L CaCl2. The content of R-1 was increased from 82.15 kU/mL to 116.27 kU/mL.

ObjectiveTo observe the clinical efficacy of duloxetine combined with psychological intervention in patients with somatoform disorder.Methods67 patients with somatoform disorder were randomly divided into two groups:study group (34 cases) treated with duloxetine and psychological intervention and control group (33 cases) treated with duloxetine only.And all the 67 patients in the study were assessed by symptom checklisth-90-R (SCL-90),hamilton anxiety scale(HAMA) and global assessment scale(GAS).ResultsAfter 8 weeks treatment,the results of SCL-90 showed that the scores of somatization,obsessive-compulsive,depression,anxiety and phobia factors in the study group ( 1.39 ± 0.45 ; 1.66 ± 0.52 ; 1.83 ± 0.69 ; 1.73 ± 0.66 ; 1.30 ± 0.48 )were notably lower than that in the control group ( 1.78 ± 0.61 ; 1.69 ± 0.47 ;2.39 ± 0.54 ; 2.1 1 ± 0.49 ; 1.49 ±0.78 ).And there were significant statistical differences (P＜ 0.01 ).At the end of the 2nd and 4th week the,scores of SCL-90 somatization,depression,anxiety and phobia factors were lower in the study group than the control group with statistical significance (P＜0.05).At the end of 8th week,the HAMA score in the study group( 10.20 ±3.70) was significantly decreased than the control group (13.07 ± 3.20 ),and with statistical significant (P ＜0.05 ).The response rate was 85.29％ in study group and 60.60％ in control group.And there was a statistically singnificant difference in complete remission ( χ2 =4.08,P ＜ 0.05 ) between the two groups.ConclusionThe study indicates that the combination of duloxetine and psychological intervention is more effective in treating somatoform disorder than using duloxetine only.So this combination treatment is worth generalizing and applying.

AIM: To study the chemical constituents from leaves of Ampelopsis sinica. METHODS: The chem-ical constituents were isolated by column chromatographic methods, and the structures were elucidated on the basisof IR,~1H-NMR, ~(13)C-NMR, MS spectral analysis. RESULTS: Nine compounds were isolated and their structures were identified as quercetin β-sitosterol,vanillic acid,3,5-dimethoxy1-4-orthohydroxybenzoic acid ,epicatechin,api-genin, kaempferol, 5,7,3',4'-Tetrahydroxyflavone-3-O-6-rhamnose, rutin. CONCLUSION: All compounds are isolated from leaves of this plant for the first time.

Objective To determine the effects of isoflurane on the amino acid neurotransmitter contents in rat cerebral cortex, hippocampus and spinal cord. Methods Sixteen male SD rats weighting 220-280g were randomly divided into two groups: isoflurane group (A) and control group (B). Animals in group A were killed after 30min inhalation of 1.3% isoflurane and cerebral cortex, hippocampus and spinal cord were removed immediately for determination of glutamic acid (Glu), aspartic acid (ASP), glutamine (Gln), GABA and glycine (Gly) levels by high-performance liquid chromatography (HPLC), whereas in group B O2 was inhaled instead of isoflurane. Results As compared with control group, Asp and Glu levels in cerebral cortex and hippocampus decreased markedly while Gly level increased significantly in hippocampus and spinal cord in isoflurane group. Conclusions The inhibition of excitatory amino acid synapse transmission and augmentation of inhibitory amino acid synapse transmission may be involved in the mechanism of isoflurane anesthesia.

Objective The aim of this study is to explore the significance of fitting hearing aids before cochle_ar implantation by studying the postoperative impact on neural response telemetry (NRT ) threshold ,the subjective threshold(T -level) and maximal comfort level(C-level) values .Methods A total of 45 patients with cochlear im_plants were divided into 3 groups:patients in group HA1(n=17)had worn hearing aids preoperatively more than 6 months and the age of starting wearing hearing aids was yonger than 3 .5 years old ,patients in group HA2(n=8) had worn hearing aids preoperatively more than 6 months and the starting age was older than 3 .5 years old ,patients in group NHA(n=20 )never wore hearing aids before ACI .The postoperative electrode impedance ,NRT value and T -level and C -level were evaluated at 3rd ,6th ,and 12th months ,respectively .ResuIts Electrode impedances were 10 .9 ± 2 .1 kΩ in NHA group ,10 .5 ± 2 .3 kΩ in HA1 group ,10 .7 ± 2 .7 kΩ in HA2 group ,respectively ,and there were no statistically significant differences .Significant differences were found between group HA1 and group NHA ,group HA1 and group HA2 in terms of NRT threshold value ,T -level or C-level(P<0 .05) .There were no statistically significant differences between group HA2 and group NHA with respect to NRT threshold values ,T-level as well as C-level(P>0 .05) .ConcIusion The results of this study showed that wearing hearing aids for a longer time before CI was beneficial for hearing rehabilitation postoperatively .

Objective To investigate the changes in mechanics of respiration during perioperative period in patients undergoing orthotopic liver transplantation (OLT) without venovenous bypass. Methods Thirty patients of grade Ⅱ～Ⅲ according to the American Society of Anesthesiologists (ASA) classification, undergoing orthotopic liver transplantation without venovenous bypass, were included in this study. The respiratory parameters measured perioperatively included average airway resistance, peak inspiratory pressure, peak expiratory flow rate, dynamic compliance, work of breathing and respiratory drive. The complications of respiratory system during postoperative period were observed. Results Average airway resistance was decreased after abdominal cavity opening, decreased significantly at 5 min new hepatic phase and increased postoperatively. Peak expiratory flow rate and dynamic compliance were increased gradually after induction and increased significantly at anhepatic phase and new hepatic phase but decreased significantly on the first day postoperatively. Work of breathing was decreased after induction and decreased significantly at anhepatic phase and postoperative period. There was no significant difference in peak inspiratory pressure during operation. Compared with the values preoperation, the respiratory drive was decreased significantly during anhepatic phase and new hepatic phase. Total 35 times of postoperative respiratory complications occurred, which included pleural effusion, atelectasis, pulmonary artery hypertension, pulmonary interstitial edema and pnermonia. Conclusions There are obvious changes in respiratory mechanics during perioperative period of OLT, especially in postoperative period. Mechanical respiratory support is essential for patients until respiratory function recovers.

Objective To evaluate the efficacy of video-assisted thoracoscopic surgery(VATS)for spontaneous pneumothorax.Methods From January 2003 to June 2006,VATS was carried out in 61 cases of spontaneous pneumothorax in our hospital.Resection or ligatation of pulmonary bullae,and pleurodesis were performed by using Endo-GIA.Results Among the cases,unilateral VATS was performed on 58 patients(an axillary small incision was made in one case),and bilateral VATS was done in 3.No conversion to open surgery or severe postoperative complications occurred in this series.Pulmonary bullae was detected in 56 of all the patients.The operation time was 35-55 min(mean,46 min)for unilateral VATS,and was 85,175,and 190 min respectively for the 3 cases of bilateral VATS.The mean blood loss for unilateral operation was 40 ml(range,30-45 ml),and the blood loss in the 3 cases of bilateral VATS was 55,60,and 200 ml.The thoracic-drainage volume was 230-500 ml(mean,390 ml)in the unilateral cases,and was 350,1030,and 1200 respectively in the 3 bilateral cases.The drainage tube was withdrawn 3-6 d(mean 4 d)later in the unilateral cases,and 3,4,and 5 d later in the 3 bilateral cases.The 61 patients were followed up for 4-24 mon(mean,8 mon),during which one patient experienced recurrent pneumothorax in 2 months and was cured by closed thoracic drainage.Conclusions VATS is safe and effective for patients with spontaneous pneumothorax.

17.The abnormal results of METLP were found in 6(100%) patients with other myopathy.Most of them showed a high concentration of lactic acid post-aerobic exercise(L2),and sustainable to after rest,or even more higher(L3).3(21.4%)patients without myopathy showed abnormal results of METLP.Conclusions In all the indexes of METLP,the value of L2/P1 is the reliable index for diagnosis mitochondrial myopathy or no-myopathy;the value of L3/L2 is the most important index in differential diagnosis of mitochondrial myopathy and other myopathy.METLP plays a certain role in the diagnosis of the mitochondrial myopathy,but the clinical and pathological data ought to be combined.

Objective To evaluate the clinical efficacy and safety of catheter ablation for persistent atrial fibrillation in patients with left ventricular dysfunction.Methods Thirty consecutive patients with symptomatic left ventricular dysfunction and left ventricular ejection fraction less than 45 percent underwent catheter ablation for persistent atrial fibrillation were enrolled.Sixty patients mathched according to age,sex,left atrial diameter and duration of atrial fibrillation but without left ventricular dysfunction were selected as the control.All patients underwent circumferential pulmonary vein isolation(CPVI) combined with ablation guided by complex fractionated atrial electrograms(CFAEs).Both parameters correlated with catheter ablation and complications in patients with left ventricular dysfunction were compared will the control.Left ventricular function and left atrial dimension at baseline and at 3 and 9 months after the ablation were evaluated by echocardiogram.Results The ablation procedurts were successful in all patients.The rate of pulmonary vein isolation,ablation time,X-ray exposure time and complications were not significantly different between the left ventricular dysfunction group and the control group(96.67% vs 98.33%,P=1.00;202.23?39.03 min vs 201.87?36.80 min,P=0.97;26.80?7.77 min vs 27.06?7.16 min,P=0.88;3.3% vs 3.4%,P=1.00).After a mean follow up of 11?1 months,73% of the patients with left ventricular dysfunction and 78% of the control remained in sinus rhythm(P=0.61).Forty percent of the left ventricular dysfurction group and 42% of the control received re-ablation respectively.Patients with left ventricular dysfunction showed significant improvement in left ventricular function(increase in ejection fraction by 7.87?4.72%,P