Objective To observe the clinical effect of Sangao Decoction combined with Azithromycin in the treatment of mycoplasmal pneumonia in children.MethodsFrom August 2013 to August 2014, 76 children with mycoplasma pneumonia were enrolled in this study.The patients were randomly divided into observation group and control group.The control group was treated with azithromycin alone, and the observation group was given the treatment of Sanao Decoction on the basis of the control group.Comparison of the two groups of clinical efficacy, fever time, cough time, tonsil disappearance time, lung rales disappear time, length of stay and the incidence of adverse reactions.ResultsThe effective rate was 94.37% in the observation group and 76.32% in the control group, which was statistically significant (P<0.05).(2.45±0.90) d, duration of coughing (5.37±1.67) d, duration of disappearance of tonsil (6.23±2.47) d, disappearance of rales (7.18±1.58) d, duration of hospital stay (10.26±1.65) d were shorter than the control group, which was statistically significant (P<0.05).The incidence of adverse reactions was not statistically significant.ConclusionThe effect of Sangao Decoction combined with azithromycin in the treatment of mycoplasma pneumonia in children can significantly shorten the symptoms and signs of the patients, the time of disappearance of the signs and the length of hospital stay, promote the rapid recovery of children, high safety, and have good clinical application value.

Objective To investigate the effect of gamma globulin combined with glucocorticoid pulse therapy in the treatment of children with refractory mycoplasma pneumonia in CRP and safety.Methods 46 cases of children with refractory mycoplasma pneumonia from February 2012 to February 2016 were selected and randomly divided into the control group and experimental group,23 cases in each group.On the basis of the regular treatment,the control group was given azithromycin,10mg/kg,qd,intravenous drip,on the basis of the control group treatment,the experimental group was treated with methylprednisolone 2mg/kg+human immunoglobulin 400mg/kg,qd,intravenous drip.The levels of serum C-reactive protein(CRP),white blood cell count(WBC),lymphocyte count(LC),therapeutic efficiency and safety were measured before and after treatment.Results After treatment,compared with the control group,the serum levels of CRP、WBC、LC were lower in the experimental group,the difference was statistically significant(P<0.05).The treatment efficiency of the experimental group(91.31％)was significantly higher than that of the control group(65.22％),the difference was statistically significant(P<0.05).There was no significant difference in the incidence of adverse reactions between the two groups.Conclusion The gamma globulin combined with glucocorticoid pulse therapy can significantly reduce the serum levels of CRP,WBC and LC in children with refractory mycoplasma pneumonia,improve clinical efficacy and the safety were good.

As an anti-epilepsy drug,gabapentin plays an important role in the treatment of neuropathic pain through a variety of central and peripheral mechanisms.Studies have shown that gabapentin has better efficacy for post-stroke pain,spinal cord injury-related pain,postherpetic neuralgia,and pain in diabetic peripheral neuropathy.For mild adverse effects,good tolerance and lack of interactions,gabapentin is an ideal drug for the treatment of neuropathic pain.

RCP is a new technique of cerebral protection that perfuses oxygenated blood from the venous system to cerebral tissue. When the corporeal circulation is arrested during the cardiovascular operation ,this method can be applied to delivery blood full of oxygen to cerebrum so as to prolong the tolerance time of cerebral tissue to ischemic injury and then gain abundant time for operation. Simultaneously,this method can reduce the reperfusion time of cerebrum significantly for a stroke patient so prolong the treatment time window of stroke and improve the treatment effect ion of medicine. This report summarizes the development and clinical application of this technique in recent twenty years and prospects its development direction in the future.

BACKGROUND:α-zearalanol, a natural phytoestrogen has the effect of anti- atherosclerosis like the estrogen but with less side effect. Therefore, it has a potential for more application in the future. OBJECTIVE: To investigate the effect of 17β-estradiol (E2) and α-zearalanol (ZAL) on coagulation and fibrinolysis in ovariectomized rats and to compare and analyze their effects. DESIGN: An observational and controlled experiment. SETTING:Department of Pathophysiology of the Capital University of Medical Sciences and the Department of Pathophsiology of the Institute of Basic Medical Sciences of Chinese Academy of Medical Sciences and Peking Union Medical College. MATERIALS: The experiment was completed at the Science Department of Experiment Animals from July to September 2003. Thirty-six healthy female Wistar rats aged 12-week old , weighing (250±10)g, clean grade were involved. The animals were divided into 4 groups, namely sham-operation control group, ovariectomy (OVX) group, OVX +E2 group, OVX+ZAL group with 9 rats in each group. METHODS: For the rats in the sham-operation control group, operation was performed without removing the ovary. For the rats in the OVX group,ovariectomy was performed and the rat models were made at the sterile condition. 1 mg/kg 17β-estradiol and 1mg/kg α-zearalanol were respectively injected intramuscularly into the rats in the OVX +E2 group and OVX+ZAL group 14 days after the operation, once every three days for 35days altogether. After the administration of 17β-estradiol or α-zearalanol for 5 weeks, rats were killed, and blood was collected through the common carotid artery and plasma was collected from it. The prothrombin time (PT)and the activated partial thromboplastin time (APTT) were determined with coagulation method. The level of fibrinogen (FG) was measured with an automatic biochemistry analyzer. Tissue factor(TF)level was determined with ELISA method, the activity of tissue plasminogen activator(t-PA)and plasminogen activator inhibitor type 1 (PAI-1)were determined with chromogenic substrate assay. At the same time, the uteri were cut off and weighed by an electronic scale to work out the uteri weight/body weight (g/kg). MAIN OUTCOME MEASURES: ① PT, APTT, FG,TF, t-PA, PAI-1; ②uteri mass/body mass RESULTS: All the 36 rats entered the stage of the result analysis. ①change of PT: it was shorter in the OVX group than that in the sham-operation control group (P ＜ 0.01 ), but it was longer than that in the OVX group after supplementation of E2 and ZAL (P ＜ 0.05-0.01 ). ② Change of FG and TF: they were significantly higher than those in the sham-operation control group (P ＜ 0.05-0.01 ). But, they were lower than those in the OVX group after supplementation of E2 and ZAL (P＜ 0.05-0.01 ). ③Change of tPA: It was significantly lower in the OVX group than that in the sham-operation control group [(0.33±0.33) μkat/L,(4.00±1.50) μkat/L,(q=9.43, P ＜ 0.01 )]. However, it was significantly higher than that in the OVX group after supplementation of E2 and ZAL [(1.83 ±0.67)μkat/L,(1.17±0.83) μkat/L, (q=13.50, P ＜ 0.01; q=5.00, P ＜ 0.05). ④ Change of PAI-1: It was significantly higher than that in the sham-operation control group [(2.33±0.67) μkat/L,(1.17±0.33) μkat/L,(q=10.5, P ＜ 0.01 )]. ⑤Uteri mass/body mass: It was significantly lower in the OYX +ZAL group than that in the OVX+ E2 group [0.66,1.96, (q=14.67, P ＜ 0.01)]. CONCLUSION: Both 17β-estradiol and α-zearalanol can resume the balance of coagulation-fibrinolysis of ovariectomized rats, suggesting that αzearalanol has a similar protective effect similar to that of 17β-estradiol on cardiovascular system. As α-zearalanol has less adverse effect on uteri enlarging than 17β-estradiol, it has a better prospect for substitution of estrogens as a natural phytoestrogen.

The clonal proliferations of multiple myeloma cells consist of several signaling pathways.It has been shown that cyclic adenosine monophosphate (cAMP) signaling pathway,an important intra-cellular messenger delivery system,is related to abnormal proliferation and apoptosis of malignant lymphoid cells including myeloma cells.Manipulating the cAMP signaling pathway could induce cell cycle arrest and apoptosis of various malignant lymphoid cells including myeloma cells,which involve mitochondrial-dependent apoptosis and cAMP-modulated changes of intra-cellular mediators.In-depth researches and analysis of cAMP-induced apoptosis in myeloma cells would provide potential targets and possible therapeutic means in the treatment of multiple myeloma.

Objective To investigate the clinical efficacy of simvastatin combined with ferulate in treatment of early diabetic nephropathy (DN).Methods 106 patients with DN were randomly divided into two groups by random number table,and each group had 53 cases.The control group was given simvastatin,while the observation group was given simvastatin combined with ferulate,and the clinical outcomes were compared between the two groups.Results The total effective rate of the observation group was 92.45％,which was significantly higher than 73.58％ of the control group(x2 =8.992,P ＜ 0.05) ; after treatment,the Upro,SCr,BUN and CRP levels of the observation group were (50.72 ± 7.74) mg/24h,(70.01 ± 8.56) μmol/L,(3.87 ± 0.54) mmol/L and (3.51 ± 0.63) mmol/L,which were significantly lower than (83.74 ± 7.92) mg/24h,(84.33 ± 9.01) μmol/L,(5.03 ± 0.62) mmol/L and (5.52 ± 0.61) mmol/L of the control group,the difference was statistically significant (t =7.73,6.59,8.62,9.30,all P ＜ 0.05).Conclusion Simvastatin combined with ferulate in treatment of early diabetic nephropathy (DN) has significant effect,and it can effectively improve the clinical symptoms and protect renal function,and it has better clinical value.

ObjectiveTo investigate the expression and effects of nitric oxide synthase in the Abeta transgenic drosophila.MethodsmRNA was obtained from 20 Abeta transgenic drosophila and 20 wild type drosophila,and cDNA was synthesized by reverse transcription.The expression of nitric oxide synthase mRNA was detected by RT-PCR.ResultsThe expression of nitric oxide synthase mRNA (0.020 ± 0.006)in the Abeta transgenic drosophila was significantly lower than that in the wild type drosophila ( 1.000 ±0.149),there had significant difference between Abeta transgenic drosophila and wild type drosophila ( P ＜ 0.05)..ConclusionsNitric oxide generated by nitric oxide synthase was an important messenger molecule,and it was closely related to Alzheimer's disease.

Objective To observe the efficacy of bioelectric stimulation of the pelvic floor combined with acupuncture in treating detrusor urine weakness after transurethral resection of the prostate (TURP) in patients with prostate hyperplasia.Methods Forty-seven cases with detrusor urine weakness after TURP were randomly divided into an experimental group and a control group.All of the patients received routine bladder function training and medication.In addition,patients in the experimental group were treated with bioelectric stimulation of the pelvic floor and with acupuncture.The differences between the two groups were compared using the International Prostate Symptom Score (IPSS),maximum urine flow rate (MFR),average flow rate (AFR),detrusor pressure at peak flow (PdetQmax) and residual urine volume in the bladder.Results No significant difference was found between the two groups before treatment,but at two weeks after treatment MFR,AFR and PdetQmax were significantly higher in the experimental group than in the control group,and residual urine volume and IPSS were significantly lower.Conclusion Bioelectric stimulation of the pelvic floor combined with acupuncture is more effective than routine bladder function training and medication alone for alleviating detrusor urine weakness after TURP.