Acupuncture Points ; Child, Preschool ; Dyspepsia ; therapy ; Female ; Fever ; therapy ; Humans ; Male ; Massage

The technique of minimally invasive embedding simplifies the clinical manipulation of embeddingtherapy and relieves painful patients. The safety of minimally invasive embedding is analyzed in terms of manipula-tion apparatuses, embedding materials, operating techniques and possible adverse reactions, etc. It is expected thatthe evidence of popularizing minimally invasive embedding should be provided.
Acupuncture Points ; Acupuncture Therapy ; instrumentation ; methods ; Animals ; Catgut ; adverse effects ; standards ; utilization ; Humans

Enzyme immobilization is the core technology of biocatalysis. Over the past few decades, enzyme immobilization research mainly focused on single enzyme immobilization. In recent years, multi-enzyme immobilization attracts more and more attention as it could increase the local concentration of reaction and improve the reaction yield. In this review, a summary of the recent progress, together with our research, is presented. Special emphasis is placed on four methods in multi-enzymes co-immobilization, namely, the nonspecific covalent co-immobilization, the nonspecific non-covalent co-immobilization, the non-covalent encapsulation co-immobilized and the site specificity co-immobilized. Finally, some industrial uses of immobilized multi-enzymes were addressed and the application prospect of multi-enzyme immobilization was highlighted.
Biocatalysis ; Enzymes, Immobilized

OBJECTIVEThis study aims to investigate the temporal pattern of expression of vascular endothelial growth factor (VEGF) and new bone formation during midpalatal suture expansion osteogenesis for rapid maxillary expansion.

METHODSA total of44 New Zealand white rabbits were randomly assigned to 11 groups, namely, five experimental groups, five control groups, and one control 0 group. A Haas appliance was used for the rapid expansion of the midpalatal suture; rapid expansion was set for 2 weeks and fixed for 4 weeks. The tissue of the maxillary midpalatal suture was harvested on the day of installing rapid expansion (control 0 group), on weeks 1 and 2 for rapid expansion, and on weeks 1, 2, and 4 for fixed (experimental group and control group, respectively). The immunohistochemical method was used to detect distribution and expression of VEGF, and new bone formation was observed with periodic acid-Schiff.

RESULTSHigher VEGF expressions are observed after midpalatal suture distraction osteogenesis. Positive staining for VEGF is mainly noted in the vascular endothelial cells, and the active osteoblasts are at the edge of a newborn trabecular bone. A weak VEGF expression is detected among cells in the control group. The VEGF expression of the experimental group is higher than that of the control group in rapid expansion weeks 1 and 2 and in fixed weeks 1 and 2. The expression of VEGF in the experimental group increases significantly and peaks at fixed week 1, and then gradually decreases. The amount of newly formed bone in the experimental groups is always higher than that in the control group; moreover, it increases significantly and peaks at fixed week 2, and then gradually decreases.

CONCLUSIONThe mechanical strain created by rapid maxillary expansion generates a sequence of VEGF cellular events that lead to increased vascularization and subsequent new bone formation.

Brain-computer interface (BCI) systems identify brain signals through extracting features from them. In view of the limitations of the autoregressive model feature extraction method and the traditional principal component analysis to deal with the multichannel signals, this paper presents a multichannel feature extraction method that multivariate autoregressive (MVAR) model combined with the multiple-linear principal component analysis (MPCA), and used for magnetoencephalography (MEG) signals and electroencephalograph (EEG) signals recognition. Firstly, we calculated the MVAR model coefficient matrix of the MEG/EEG signals using this method, and then reduced the dimensions to a lower one, using MPCA. Finally, we recognized brain signals by Bayes Classifier. The key innovation we introduced in our investigation showed that we extended the traditional single-channel feature extraction method to the case of multi-channel one. We then carried out the experiments using the data groups of IV-III and IV - I. The experimental results proved that the method proposed in this paper was feasible.
Bayes Theorem ; Brain ; physiology ; Brain-Computer Interfaces ; Electroencephalography ; Humans ; Magnetoencephalography ; Multivariate Analysis ; Principal Component Analysis

Amino Acid Metabolism, Inborn Errors ; etiology ; therapy ; Glutarates ; blood ; Humans ; Infant, Newborn ; Male

Objective To evaluate the dosimetric benefits of intensity-modulated radiotherapy (IMRT) for early stage breast cancer after breast-conserving surgery. Methods Ten patients with stage Tis～2N0M0 breast cancer receiving breast-conserving surgery were selected for the study. Six cases were on the left, four were on the right. A dosimetric comparison of IMRT with conventional wedged tangential technique was performed on each patient using three dimensional treatment planning. The total prescribed dose for both plans was 50 Gy/25 fractions. Dose volume histograms (DVH) were used to compare the planning target volume (PTV) and organs at risks(OARS). Results The PTV coverage in IMRT plan was similar to that of the conventional plan (97.83 % vs 97.61%). A better dose uniformity throughout the whole breast was achieved by IMRT plan. The percentage of PTV receiving less than 95 % prescribed dose and more than 103 % prescribed dose (inhomogeneity index IHI), decreased from 25.42 % to 2.71%: the percentage of PTV receiving more than 105 % prescribed dose (V105 %) decreased from 25.79 % to 1.08 %. A better amelioration of IHI and reduction of V105 % in IMRT plans were observed in the relatively large PTV subgroup. The Dmean and percentage of volume receiving more than 30 Gy (V30) of heart from 6.72 Gy to 4.95 Gy, and from 7.23 % to 1.04 %. the Dmean and percentage of volume receiving more than 20 Gy (V20) of ipsilateral from 9.19 Gy to 7.65 Gy, and from 22.34 % to 20.18 %; the Dmean of contralateral breast decreased from 5.12 Gy to 3.52 Gy;the Dmean of contralateral lung decreased from 2.76 Gy to 1.79 Gy; the Dmean of liver decreased from 7.23 Gy to 1.04 Gy for right side patients. Conclusion Forward planning IMRT based on a standard tangential beam arrangement significantly improves the dose homogeneity throughout the target volume of intact breast and reduces the dose to OARS, especially in patients with large breast volumes who might be proposed as candidates of IMRT for intact breast.

Objective To investigate the stability of glycated hemoglobin HbA1c in whole blood sample measured by Tosoh G7, Roche/Hitachi 7170A and NycoCard READER Ⅱ under different storage conditions. Methods Three whole blood samples (EDTA anticoagulated) with different glycated hemoglobin levels and one whole blood sample (heparin anticoagulated) were collected and stored at -80 ℃, -20 ℃, 4 ℃,room temperature(15 -25 ℃), and 37 ℃ HbA1c was analyzed by each method on days 1, 2, 5, 7, 9, 14, 21,28, 35, 42, 49, 56, 63 and 70 respectively. Results The results of sample stored at -80 ℃ appear to be stable for Tesoh G7 and Roche/Hitachi 7170A method. The coefficients of variation (CV) for Tosoh G7 was 0.54%-1.22%. The CV for Roche/Hitachi 7170A was 0.86% -1.82%. When samples was detected with Tosoh G7 method, the results was consistent when the sample was stored at -20 ℃ for 14 days, 4 ℃ for 63 days, room temperature for 5 days, and 37 ℃ for less than 1 day. When samples was detected with Roche/Hitachi 7170A method, the results was consistent when the samples was stored at -20 ℃ for 21 days, 4 ℃ for 42 days, room temperature for 7 days, and 37 ℃ for less than 1 day. The NycoCard READER Ⅱ showed stability at 4 ℃ for 9 days, and room temperature for less than 1 days. Conclusions The stability of whole blood samples is dependent on different methods. Storage time under different temperatures is different.

Objective Approach mammographic wire-localization biopsy technology's value in diagnose of dditescenee focus of infection about breast disease. Methods Utilizing specific purpose computer stereotaxis system, utilize mammographic wire-localization for 47 pieces delitescenee focus of infection about breast disease which can't be touehed in clinical ,at guide wire's leading exsect focus of infection and make molybdenum target for tissue by ex- cisional, then make patho-test when the focus of infection were completely resection. Results 45 patients have 47 pieces delitescence focus of infection,detaining 47 guide wire,all operations succeed only by once,and the focus of in- fection are completely resection,the correct rate of diagnose is 100% .The result of patho:13 cases are breast cancer and that have 14 pieces focus of infection,one of them is both sides,benign lesion are 32 cases and 33 pieces focus of infection. Conclusion The biopsy by mammographie wire-localization is convenient and accurate, can make sure the character of delitescence focus of infection about breast disease,which cart raise the rate of diagnosis in earlier period breast cancer.

Objective　To observe the effects of repeated subconvulsive electrical stimulations to hippocampus on rat's emotional behavior and spatial learning and memory. Mehtods　The hippocampus of Wistar rats were repeatedly stimulated by constant pulsating current with intratrain frequencies of 16 Hz, pulsating duration of 1 ms, train duration of 10 s and interstimulus interval of 7 min for 5 days. Results　The experimental rats displayed the long-term significant abnormalities of emotional behaviors, such as substantial changes of behavioral habits, hyperarousal, hypersensitive frightened reactions, anxiety-like behaviors, and transitory spatial learning and memory disorder. Conclusion　The stable and practical animal model satisfied most characteristics of posttraumatic stress disorder (PTSD).