Acupuncture Points ; Child, Preschool ; Dyspepsia ; therapy ; Female ; Fever ; therapy ; Humans ; Male ; Massage

Enzyme immobilization is the core technology of biocatalysis. Over the past few decades, enzyme immobilization research mainly focused on single enzyme immobilization. In recent years, multi-enzyme immobilization attracts more and more attention as it could increase the local concentration of reaction and improve the reaction yield. In this review, a summary of the recent progress, together with our research, is presented. Special emphasis is placed on four methods in multi-enzymes co-immobilization, namely, the nonspecific covalent co-immobilization, the nonspecific non-covalent co-immobilization, the non-covalent encapsulation co-immobilized and the site specificity co-immobilized. Finally, some industrial uses of immobilized multi-enzymes were addressed and the application prospect of multi-enzyme immobilization was highlighted.
Biocatalysis ; Enzymes, Immobilized

OBJECTIVEThis study aims to investigate the temporal pattern of expression of vascular endothelial growth factor (VEGF) and new bone formation during midpalatal suture expansion osteogenesis for rapid maxillary expansion.

METHODSA total of44 New Zealand white rabbits were randomly assigned to 11 groups, namely, five experimental groups, five control groups, and one control 0 group. A Haas appliance was used for the rapid expansion of the midpalatal suture; rapid expansion was set for 2 weeks and fixed for 4 weeks. The tissue of the maxillary midpalatal suture was harvested on the day of installing rapid expansion (control 0 group), on weeks 1 and 2 for rapid expansion, and on weeks 1, 2, and 4 for fixed (experimental group and control group, respectively). The immunohistochemical method was used to detect distribution and expression of VEGF, and new bone formation was observed with periodic acid-Schiff.

RESULTSHigher VEGF expressions are observed after midpalatal suture distraction osteogenesis. Positive staining for VEGF is mainly noted in the vascular endothelial cells, and the active osteoblasts are at the edge of a newborn trabecular bone. A weak VEGF expression is detected among cells in the control group. The VEGF expression of the experimental group is higher than that of the control group in rapid expansion weeks 1 and 2 and in fixed weeks 1 and 2. The expression of VEGF in the experimental group increases significantly and peaks at fixed week 1, and then gradually decreases. The amount of newly formed bone in the experimental groups is always higher than that in the control group; moreover, it increases significantly and peaks at fixed week 2, and then gradually decreases.

CONCLUSIONThe mechanical strain created by rapid maxillary expansion generates a sequence of VEGF cellular events that lead to increased vascularization and subsequent new bone formation.

Amino Acid Metabolism, Inborn Errors ; etiology ; therapy ; Glutarates ; blood ; Humans ; Infant, Newborn ; Male

Brain-computer interface (BCI) systems identify brain signals through extracting features from them. In view of the limitations of the autoregressive model feature extraction method and the traditional principal component analysis to deal with the multichannel signals, this paper presents a multichannel feature extraction method that multivariate autoregressive (MVAR) model combined with the multiple-linear principal component analysis (MPCA), and used for magnetoencephalography (MEG) signals and electroencephalograph (EEG) signals recognition. Firstly, we calculated the MVAR model coefficient matrix of the MEG/EEG signals using this method, and then reduced the dimensions to a lower one, using MPCA. Finally, we recognized brain signals by Bayes Classifier. The key innovation we introduced in our investigation showed that we extended the traditional single-channel feature extraction method to the case of multi-channel one. We then carried out the experiments using the data groups of IV-III and IV - I. The experimental results proved that the method proposed in this paper was feasible.
Bayes Theorem ; Brain ; physiology ; Brain-Computer Interfaces ; Electroencephalography ; Humans ; Magnetoencephalography ; Multivariate Analysis ; Principal Component Analysis

Based on the subtle discussion of changing rules and its principles of Six Qi movement recorded by one chapter named Liu Wei Zhi Da Lun in the Huang Di Nei Jing Su Wen about rules of Heaven divided by Six, Six Qi and year evolutive phase, Six Qi dominating the time, and guest climatic Qi adding to fixed host Qi, etc, this article explained the changes of influence of Heaven space-time on the human body from the angle of image thinking, focused on the key point of humanity conforming to the nature of the core of Chinese philosophy, and appealed readers to come back to the original thinking of China-image thinking.

Objective To investigate the effects of meglumine adenosine cyclophosphate administrated before percutaneous coronary intervention (PCI) on curative efficacy, cardiac function and oxidative stress in patients with acute myocardial infarction (AMI).Methods118 patients of AMI meeting the inclusion criteria were selected as research objects.According to therapeutic schemes, those patients were divided into the control group (n=52) and the observation group (n=66).Before PCI, the control group was given conventional treatments.Besides that, the observation group was given meglumine adenosine cyclophosphate.Then, the curative efficacy, indices of cardiac function and oxidative stress were compared.ResultsAfter the treatments, the observation group had higher rates of ST decrease (>50%), thrombolysis in myocardial infarction(TIMI)3 grade (90.9% vs76.9%,84.8% vs 69.2%, P<0.05) and lower peak concentration of creatine kinase isoenzyme(CK-MB), cardiac troponin(cTn)I[(140.6±26.5) U/L比(193.7±30.8) U/L,(1.92±0.24)μg/L 比(2.71±0.32) μg/L,P<0.05].After the treatment, indices of stroke volume (SV), cardiac output (CO) and left ventricular ejection function (LVEF) which reflect cardiac function in the observation group were statistically higher than those in the control group [(57.26±9.37)mL vs.(52.16±9.15)mL,(5.23±1.17) L/min vs(4.49±1.12)L/min,(0.62±0.12) % vs.(0.51±0.15)%,P<0.05].24 hours after PCI, as to oxidative stress, the observation group had a statistically lower level of malondialdehyde(MDA) and higher level of superoxide dismutase[(4.39±0.43)nmol/L vs.(6.45±0.46) nmol/L, [(87.12±10.34)U/mL vs.(68.29±9.48)U/mL), P<0.05].ConclusionMeglumine adenosine cyclophosphate administrated before PCI is effective for patients with AMI.And it can significantly improve cardiac function and alleviate oxidative stress.

The techniques for endoscopic diagnosis and treatment of biliary and pancreatic diseases mainly include endoscopic retrograde cholangiopancreatography and endoscopic ultrasonography,which have achieved great progress in recent years.Meanwhile,new techniques including combined therapy with endoscopy and laparoscopy and natural orifice transluminal endoscopic surgery have gradually been applied in clinical practice.In the future,it is necessary to cultivate interdisciplinary experts in the field of endoscopy who master various endoscopic techniques and integrated talents with experience in both transluminal operation and gastrointestinal surgery outside lumens.At present,there are still controversies over the application of new techniques in the treatment of biliary and pancreatic diseases.The diagnosis and treatment of biliary and pancreatic diseases can be further improved by promoting the application of biliary and pancreatic endoscopy and standardization,accumulating experience and improving devices,evaluating clinical research,and promoting the bench-to-bedside translation of new techniques.

Objective:To explore the effects of formative assessment using sitcom play in obstetrics and gyne-cology nursing teaching.Methods:Totally 214 undergraduate of grade 2011 nursing students were recruited in the present study.Using envelope method,2 classes were randomly selected as the control group and 2 as the experi-mental group.Students in the control group (n--107) were assessed with traditional assessment method,while those in the experimental group (n =107) were taught using situational drama performance and assessed with form-ative assessment method throughout the process.Students' test scores of obstetrics and gynecology nursing in these two groups were compared,and the opinions on the formative assessment method using sitcom play were investiga-ted among students in the experimental group.Results:Students in the experimental groupscored significantly high-er than those of the control group(78.43 ± 5.21 vs.76.67 ± 6.40,t =2.20,P ＜ 0.05).About 98.31％ of the students approved the formative assessment mode.They reported that this approach was helpful to improve the abili-ty to combine theory and practice andto communicate with others,to cultivate students' creative thinking,and to stimulate their learning interest and learning initiative.Conclusion:The application of formative assessment mode using sitcom play in obstetrics and gynecology nursing teaching is beneficial to enhance teaching effect and improve the ability of nursing students,which is worth popularizing.

Objective To explore the effect of the application of cervical loop electric knife (LEEP) in the patients with cervical micro invasive cancer.Methods Clinical data of 66 patients with minimal invasive carcinoma diagnosed by LEEP cone resection in our hospital from June 2013 to June were retrospectively analyzed. Preoperative and postoperative patients with LEEP were compared with 66 patients who underwent biopsy with biopsy, the diagnosis of micro invasive carcinoma was compared with LEEP cone resection.Results The results of colposcopy biopsy were detected in 2 cases of LSIL, 60 cases of HSIL and 1 cases of AIS, 3 cases were diagnosed as cervical microinvasive carcinoma or suspicious cervical microinvasive carcinoma, the sensitivity was 4.54%, the misdiagnosis rate was 95.46%; LEEP conization were detected in 1 cases of LSIL, 44 cases of HSIL and 1 cases of AIS, 20 cases negative margin. There was no significant difference in the thickness and area of the resected tissue between the three groups (P > 0.05). The depth of the resected tissue was significantly higher than that in the HSIL and AIS group, the HSIL and AIS groups were significantly higher than the LSIL group, the difference was statistically significant (P < 0.05). Interstitial infiltration depth is less than or equal to 1mm group, the 1 depth of stromal invasion than 3mm group and 3 < rate were 15.00%, 19.51% and 20.00% of the remaining lesion depth of stromal invasion than the patients in the 5mm group after operation, there was no statistically significant difference (P > 0.05). Cut edge positive group, positive margin group and interstitial fiber positive margin group of patients with postoperative residual disease rates were 25.00%, 15.38% and 23.80%, the difference was not statistically significant (P > 0.05).Conclusion The rate of missed diagnosis of small invasive cervical cancer is higher, and the diagnosis rate can be improved by the combination of LEEP and cone resection.