Acupuncture Points ; Child, Preschool ; Dyspepsia ; therapy ; Female ; Fever ; therapy ; Humans ; Male ; Massage

OBJECTIVEThis study aims to investigate the temporal pattern of expression of vascular endothelial growth factor (VEGF) and new bone formation during midpalatal suture expansion osteogenesis for rapid maxillary expansion.

METHODSA total of44 New Zealand white rabbits were randomly assigned to 11 groups, namely, five experimental groups, five control groups, and one control 0 group. A Haas appliance was used for the rapid expansion of the midpalatal suture; rapid expansion was set for 2 weeks and fixed for 4 weeks. The tissue of the maxillary midpalatal suture was harvested on the day of installing rapid expansion (control 0 group), on weeks 1 and 2 for rapid expansion, and on weeks 1, 2, and 4 for fixed (experimental group and control group, respectively). The immunohistochemical method was used to detect distribution and expression of VEGF, and new bone formation was observed with periodic acid-Schiff.

RESULTSHigher VEGF expressions are observed after midpalatal suture distraction osteogenesis. Positive staining for VEGF is mainly noted in the vascular endothelial cells, and the active osteoblasts are at the edge of a newborn trabecular bone. A weak VEGF expression is detected among cells in the control group. The VEGF expression of the experimental group is higher than that of the control group in rapid expansion weeks 1 and 2 and in fixed weeks 1 and 2. The expression of VEGF in the experimental group increases significantly and peaks at fixed week 1, and then gradually decreases. The amount of newly formed bone in the experimental groups is always higher than that in the control group; moreover, it increases significantly and peaks at fixed week 2, and then gradually decreases.

CONCLUSIONThe mechanical strain created by rapid maxillary expansion generates a sequence of VEGF cellular events that lead to increased vascularization and subsequent new bone formation.

Enzyme immobilization is the core technology of biocatalysis. Over the past few decades, enzyme immobilization research mainly focused on single enzyme immobilization. In recent years, multi-enzyme immobilization attracts more and more attention as it could increase the local concentration of reaction and improve the reaction yield. In this review, a summary of the recent progress, together with our research, is presented. Special emphasis is placed on four methods in multi-enzymes co-immobilization, namely, the nonspecific covalent co-immobilization, the nonspecific non-covalent co-immobilization, the non-covalent encapsulation co-immobilized and the site specificity co-immobilized. Finally, some industrial uses of immobilized multi-enzymes were addressed and the application prospect of multi-enzyme immobilization was highlighted.
Biocatalysis ; Enzymes, Immobilized

Brain-computer interface (BCI) systems identify brain signals through extracting features from them. In view of the limitations of the autoregressive model feature extraction method and the traditional principal component analysis to deal with the multichannel signals, this paper presents a multichannel feature extraction method that multivariate autoregressive (MVAR) model combined with the multiple-linear principal component analysis (MPCA), and used for magnetoencephalography (MEG) signals and electroencephalograph (EEG) signals recognition. Firstly, we calculated the MVAR model coefficient matrix of the MEG/EEG signals using this method, and then reduced the dimensions to a lower one, using MPCA. Finally, we recognized brain signals by Bayes Classifier. The key innovation we introduced in our investigation showed that we extended the traditional single-channel feature extraction method to the case of multi-channel one. We then carried out the experiments using the data groups of IV-III and IV - I. The experimental results proved that the method proposed in this paper was feasible.
Bayes Theorem ; Brain ; physiology ; Brain-Computer Interfaces ; Electroencephalography ; Humans ; Magnetoencephalography ; Multivariate Analysis ; Principal Component Analysis

Amino Acid Metabolism, Inborn Errors ; etiology ; therapy ; Glutarates ; blood ; Humans ; Infant, Newborn ; Male

Objective To study the effect of nebulized inhalation of ipratropium bromide and ambroxol hydrochloride (mucosolvan) on pulmonary function in elderly patients who need thoracotomy.Methods From June 2011 to December 2011,a total of 60 elderly patients undergoing thoracotomy were randomly divided into 2 groups:the treatment group (n =30) and control group (n=30).The patients in treatment group were treated with ipratropium bromide 0.5 mg and mucosolvan 15 mg diluted in 5 ml saline for inhalation with an oxygen flow rate of 6 L/min,15 min,3 times/d,and the patients in control group were treated with ipratropium bromide 0.5 mg diluted in 5 ml saline for inhalation with an oxygen flow rate of 6 L/min,15 min,3 times/d.The resting lung function in patients was measured before nebulization treatment.After 3 days treatment,the resting lung function was measured again,and vital capacity (VC),forced expiratory volume in one second (FEV1),FEV1 ％,maximal voluntary ventilation (MVV) were recorded.Results After 3 days treatment,the indicators of lung function including VC,FEV1,FEV1 ％,MVV were significantly increased in both groups (P＜0.01).The increase in VC,FEV1,FEV1 ％,MVV were significantly higher in the treatment group [(0.39±0.08)L,(0.20±0.10)L,(10.61±4.66)％,(10.24±5.07)L,respectively] than in control group [(0.31±0.05)L,(0.13±0.06)L,(7.40±3.89)％,(6.76±3.70)L,respectively] (t=4.81,3.64,4.13,3.48,all P＜0.05).The incidence of postoperative pulmonary complications was lower in treatment group[16.7 ％ (5/30)] than in control group [30.0 ％ (9/30)] (x2=11.45,P＜0.05).Conclusions The nebulized inhalation of ipratropium bromide and ambroxol hydrochoride can improve the lung function in elderly patients undergoing thoracic surgery,better withstand the thoracotomy and decrease the incidence of postoperative pulmonary complications.

Objective To explore the community nursing talents' occupation quality demand in the purpose of periodically providing reference for higher medical institutions' nursing education and for job training of community nurses.Methods The community nurses in the 15 qualified communities' medical organizations in the city of Zunyi were investigated through self-designed questionnaires,analyzing the basic qualifications,humanistic knowledge,professional knowledge and profession ability demand that community nursing talents were supposed to possess.Results The demand of high occupation responsibility and strict working style amounted to a relatively large proportion among the 11 basic qualifications.Demand of nursing etiquette and community nursing management occupied a relatively large proportion among the 9aspects of community nurses' humanistic knowledge demands.Demand of the often used community nursing technical operation and common drugs' clinical application knowledge took up a relatively large proportion among the 16 community nurses' professional knowledge demands.Demand of interpersonal and communication skills and self protection ability held a relatively large proportion among the 14 community nursing talents' occupation ability demands.Conclusions Oriented to the demand of the community nursing talents' occupation quality demands,relevant higher medical institutions or colleges concerned should strengthen the basic quality,optimize the curriculum structure and improve occupation ability in the purpose of culturing the community nursing talents who are able to adapt to the demand of community health service and have higher overall qualities as well.

Objective　To study the characteristics of hearing loss caused by local microcirculatory disorders in the cochlea of guinea pigs. Methods　The local microcirculatory disorders in the cochlea basal turn near terminal or in the cupule of cochlea of guinea pigs were induced by photochemical reaction. The morphological changes in the cochlea were observed with light microscopy. The compound action potential N1 (CAPN1) amplitude, latency, threshold shift evoked by short tone burst were recorded by Madsen 2250 system. Results　When the local microcirculatory disorders took place in the cochlea basal turn near terminal, the hearing losses were more remarkable in the high-range frequencies, but with low-range ones to some extent. If the local microcirculatory disorders was induced in the cochlea cupule, the hearing losses were mainly in the low-range frequencies companying with some high-frequency ones. Conclusion　Local microcirculatory disorders in the different parts of the cochlea cause different types of hearing loss in different frequency.

Objective　To investigate the effects of escharectomy at different time points on myocardial damages in scalded rats during shock stage. Methods　A total of 42 rats were inflicted with 30% TBSA Ⅲ scalding on their backs and then randomly and equally divided into 7 groups: burned control group (C) and escharectomy groups at hour 1, 3, 6, 12, 24 and 36 postburn. Another 6 rats were employed as normal control (N). The serum levels of creatine kinase isoenzymes MB (CKMB), aspartate aminotransferase (AST) were determined and morphological changes in myocardial tissues were observed 72 hours after the burn. Results　The serum levels of CKMB and AST were higher in the C group than in other groups, and the levels in escharectomy groups were increased along with the prolongation of scar cutting. Pathologically, the myocardial tissues were severely damaged in C group, and the later the scars were cut, the severe the damages were in the escharectomy groups except the 2 groups with scars cut at hour 1 and 3. Conclusion　Escharectomy in shock stage can effectively prevent the damage of the myocardial tissues from postburn injury and the earlier it is performed, the better the result is.

Objective　To study the cochlea microvasculature permeability changes of the inner ear in guinea pigs, so as to clarify the possible role of the cochlea microvasculature permeability changes in the ischemic injury of inner ears. Methods　Modified method of Evans blue fluorescence was used to observe the changes of permeability of the cochlea microvasculature in the animal model of cochlea microcirculatory disorders which was caused by photochemical reaction. CAPN1 threshold was recorded by using Madsen 2 250 to study the hearing loss. Results　The amounts of Evens blue crossing the cochlea microvasculature were (1.709±0.769) and (2.849 ±0.653) μg/per animal 2 and 4 h after the development of cochlea microcirculatory disorder in guinea pigs, respectively (P<0.01); and their hearing loss were （24.44 ±7.27） and （38.33±7.91）dBpeSPL in 2 and 4 h, respectively (P<0.01)． Conclusion　The permeability of the cochlea microvasculature increases along with the duration of cochlea microcirculatory disorder occur and the increase of cochlea microvasculature permeability might be one of the important mechanisms inducing cochlear ischemic lesions.