1.Effectiveness of relevant examination on visual function assessment and retinal disease detection before cataract surgery
International Eye Science 2015;(2):342-345
To evaluate the effectiveness of applying visual electrophysiological examination, including flash visual evoked potentials ( FVEP ) , flash electroretinogram ( FERG ) , pattrn electroretinogram ( PERG ) , fundus photography, eye B-type ultrasound, optical coherence tomography ( OCT ) , on visual function assessment and retinal disease detection before cataract surgery. METHODS: Visual elctrophysiological examination, fundus photography, eye B-type ultrasound and OCT were applied in 148 cases ( 196 eyes ) . The positive rate was analyzed according to the results of FVEP, FERG ( or PERG ) , fundus photography, eye B - type ultrasound and OCT.RESULTS: ln 148 cases ( 196 eyes ) , vitreous opacities was showed in 165 eyes ( 84. 2%) by eye B - type ultrasound examination, 31 eyes (15. 8%) without access to B-type ultrasound image; success for fundus camera images in 161 eyes (80. 6%), and unable to get image in 35 eyes (19. 4%); the VEP and ERG in 58 eyes (29. 6%) were get valuable, and the VEP and ERG were saw no obvious abnormal in 138 eyes ( 70. 4%); success for OCT image in 167 eyes (85. 2%), without access to OCT image in 29 eyes (14. 8%). CONCLUSlON: Visual elctrophysiological detection, fundus photography, eye B-type ultrasound and OCT can predict the preoperative visual acuity of cataractous patients. A variety of inspection methods complement each other, can further improve detection rate of retinal diseases and other eye disease before cataract surgery.
2.Relationships between insulin resistant and hypertension and traditional Chinese medicine intervention.
Journal of Integrative Medicine 2010;8(12):1114-8
Recently many researches have demonstrated that insulin resistant (IR), as an important pathologic process, is increasingly involved with a series of diseases such as central obesity and diabetes. Hypertension is highly associated with IR. The relationships between IR and glucose or lipid metabolism disorders are well established. However, association of IR with hypertension is less covered. Current researches on insulin resistance and pathogenesis of hypertension are reviewed in this paper, which holds that traditional Chinese medicine treatment on IR in patients with hypertension has a good prospect.
3.High frequency ultrasonographic diagnosis of cubital tunnel syndrome
Yi CHENG ; Weimin CHEN ; Yi WANG
Chinese Journal of Medical Imaging Technology 2009;25(12):2254-2257
Objective To assess the value of high-resolution ultrasonography in diagnosis of cubital tunnel syndrome. Methods Forty-two patients (43 elbows) initially diagnosed as cubital tunnel syndrome underwent ultrasonography (US), while 15 healthy contralateral elbows of these patients taken as self controls, and 15 healthy volunteers as normal controls underwent US of the ulnar nerve. The findings of US measurements were compared with that of intra-operative results and pre-operative electromyography. Results High-resolution US displayed changes and some etiological factors of cubital tunnel syndrome. The measurements of ulnar nerve at the proximal part of the compression were higher than those in the control groups. The cut-off point of cross-sectional area (CSA) and CSA tumefaction ratio was 0.11 cm~2 and 141.50%, respectively. The sensitivity of US was 92.86% compared with intra-operative results, and was 100% when combined with pre-operative electromyography. Conclusion High-frequency ultrasound can be used as an effective method to diagnose cubital tunnel syndrome.
5.Hepatocyte regeneration following different cold preservation durations after rat partial liver transplanta-tion
Guodong WANG ; Yi MA ; Guihua CHEN
Chinese Journal of Organ Transplantation 2008;29(9):536-539
Objective To study the molecular mechanisms of hepatoeyte regeneration following different cold preservation (CP) durations after rat partial liver transplantation. Methods Mate inbred Lewis rats were used as donors and recipients. Donor liver was kept in 4℃ UW solution for 1 h (coldisehemia 1 h group, CI 1 h group), 8 h (CI 8 h group) and 16 h (CI 16 h group) and then implantedorthotopieally. 50% liver graft transplantation model was established by ligating the left portion ofmedian lobe, left lateral lobe and caudate lobe with 3-O silk suture prior to reperfusion. Survival rate ofeach group and hepatoeyte regeneration were recorded after grafting. Reverse transcription-polymerasechain reaction was used to detect the expression of IL-6 and TNF-α in the liver tissues. Western blotanalysis was done to measure STAT3 activation in the liver. Immunohistoehemistry was conducted toanalyze the expression of cyclin D1 and hepatocyte replication with BrdU uptake in the graft. ResultsOperative success rate in all groups was 100%. Compared with CI 1 h group, the TNF-α and IL-6expression (F=67.45 for TNF-a comparison, P<0.05 and F=287.73 for IL-6 comparison,P<0.05 respectively) in 8 h CI and 16 h CI groups was markedly increased after partial grafttransplantation. STAT3 activity in 8 h C1 and 16 h C1 groups was also significantly increased ascompared with that in 1 h CI group. Cyclin D1 expression in 8 CI group was demonstrated withcytoplasmic and nuclear staining at 24 h after transplantation. Grafts in 16 h CI group showed largeareas with no cyclin D1 expression. Number of hepatocytes with BrdU positively stained neclei in 8 hCI group was more than that in 16 h C1 group at 24 h after transplantation (t=19.40, P<0.05).Conclusion Hepatocytes regeneration was present following rat partial transplantation in the graftspreserved for limited time, which may be regulated by TNF-α/IL-6' STAT3/ Cyelin D1/DNAsynthesis pathways; Hepatocytes could not respond to early signals for liver graft regeneration when50%liver graft preserved for 16 h.
6.A rat liver transplantation model of 20% small-for-size liver graft
Guodong WANG ; Yi MA ; Guihua CHEN
Chinese Journal of Hepatobiliary Surgery 2008;14(10):717-719
Objective To explore the microsurgical techniques in establishing an orthotopic rat liver transplantation model of 20% small-for-size graft. Methods A rat liver transplantation model of 20% small-for-size liver graft was orthotopically performed. Forty male Lewis rats were used as 20 donors and 20 recipients. Donor liver was perfused with 4℃ UW solution via donor portal vein. Su-prahepatic inferior vena cava was anastomosed end-to-end with running suture. Infrahepatic inferior vena cava and portal vein were reconstructed by cuff technique. A sleeve anastomosis for hepatic artery was used. Continuity of the bile duct was established with an end-to-end intraluminal stent. Survival rate of the recipients was recorded and immunohistochemical analysis of hepatocyte replication con-firmed by bromodeoxyuridine (BrdU) uptake. Results Totally 20 liver transplantations of 20% par-tial liver graft were successfully performed. The successful rate of the operation was 100%. Survival rate of the recipients with 20% liver grafts was 93.8% (>14 d). Histological examination showed normal liver structure with limited injury after transplantation. The number of positively stained nuclei was significantly increased at 72 h after transplantation. Conclusion The 20Z% small-for-size liver grafts initiate and complete the process of liver regeneration after transplantation. Skillful microsur-gery is the key to success of the transplantation model. The stable model is suitable for basic research in partial liver transplantation.
7.Clinical analysis on 34 cases with uterine sarcoma
Weifang CHEN ; Jiangyu ZHANG ; Yi WANG
The Journal of Practical Medicine 2016;32(16):2692-2694
Objective To analyze the clinical features of the uterine sarcoma with different histological types and improve the capability of diagnosis and therapy. Methods Thirty-four cases with uterine sarcoma treatment were analyzed respectively , among which there were 19 cases with malignant endometrial interstitial sarcomas (55.8%), 6 cases with leiomyosarcoma (18%), 9 with malignant mixed tumor (26%). Results (1) The average age of patients were about 46 , patients with endometrial interstitial sarcomas aged 28 ~ 60 were more common in relatively younger , and patients with malignant mixed tumor aged about 56 were more common in postmenopausal women. Incidence rate of patients with endometrial interstitial sarcomas were more common (55.8%). (2) The patients usually manifested with abnormal vaginal bleeding (76%). Diagnosis curettage were the most commonly used before operation, which the positive rate was 65.3% and postoperative pathological di-agnosis was 35%. (3) 26 patients underwent one stage surgical treatment.7 patients underwent two stage surgical treatment. Surgical methods were the removal of the uterine double accessories and pelvic lymph node dissection. The five year survival rate was 77.7% (14/18). Conclusions The age range of uterine sarcoma is more exten-sive. Preoperative diagnosis can be diagnosed by curettage, and may also be missed. It should be paid attention to the operation of the examination examination , timely delivery of frozen examination to improve the diagnostic rate. and the appropriate surgical choice are meaningful methods to improve the prognosis.
8.Molecular mechanisms of liver regeneration following cold ischemia injury after liver transplantation in rat
Guodong WANG ; Yi MA ; Guihua CHEN
Chinese Journal of Pathophysiology 2000;0(08):-
14 d).Compared with 1 h CI,TNF-? expressions in whole liver grafts with 8 h and 16 h CI were markedly increased at 90 min after reperfusion(P
9.Influence of preoperative transcatheter arterial chemoembolization on liver resection in patients with resectable hepatocellular carcinoma
Yunquan LUO ; Yi WANG ; Han CHEN
Chinese Journal of General Surgery 1993;0(03):-
ObjectiveTo study the influence of preoperative transcatheter arterial chemoembolization (TACE) on liver resection in patients with resectable hepatocellular carcinoma (HCC).MethodsTACE was performed before liver resection in 62 out of 126 patients, and perioperative risk factors were compared with that of the 64 patients without TACE including liver perioperative function alterations, average blood loss during operation, the average time of clamping porta hepatis and operation, abdominal drainage at 1-, 2-, and 3-days post-op, mortality and morbidity. ResultsThere was not significant difference in liver function alteration in the two groups, there was no mortality in the two groups. Liver cirrhosis in TACE group was more serious than that in no TACE group, the operation time was longer in TACE group. Blood loss, and abdominal drainage were much more in TACE group than in no TACE group. However postoperative complications did not differ.Conclusion Preoperative TACE for resectable HCC increases surgical difficulty and risk. Preoperative TACE for resectable HCC needs to be used on baseis of strict selection.
10.Prevention and therapy of atherosclerosis in childhood.
Yong-Hui YU ; Yao CHEN ; Yi WANG
Chinese Journal of Pediatrics 2005;43(7):547-550
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