Objective To identify the stem cell characteristics of human diploid cells 2BS and evaluate the potential as biological function positive cells for quality detection of stem cells.Methods Flow cytometry was applied to detect the expression levels of stem cell surface markers in 2BS cells.The staining with alizarin red S,oil red O and alcian blue was used to evaluate the ability of osteogenic,adipogenic and chondrogenic differentiation,respectively.Co-culture with lymphocytes was used to observe the effects on lymphocyte proliferation.Results Human diploid cells 2BS expressed the surface markers CD73,CD 105 and CD90 of stem cells,and possessed the potential of osteogenic differentiation and inhibiting lymphocyte proliferation.Conclusion 2BS cells have several properties of stem cells and could be the positive control for surface markers detection of stem cells by flow cytometry.

IL-8 is a cytokine which belongs to the chemotactic superfamily.It is one of the major mediators in the inflammatory response.In recent years,it was found that it had multiple functions,such as chemoattractant,potent angiogenic,and so on.The cytokine is believed to play an important role in the pathogenesis of inflammation,tumor progress and immune disease.In this review,the properties of IL-8 and highlight its roles on inflammation and tumor progress were surveied.

Independent component analysis (ICA) aims at decomposing an observed random vector into statistically independent variables. Fast independent component analysis (FastICA) algorithm and its variants are catching more attention because of their simplicity and convergence speed. In this paper, a novel method referred to robust independent component analysis (RobustICA), based on normalized kurtosis and optimal step-size, is analyzed in detail. When applied to fetal electrocardiogram (FECG) extraction and compared with FastICA, it gave decent results and showed prosperous future usages.
Algorithms ; Electrocardiography ; Female ; Fetal Heart ; physiology ; Fetal Monitoring ; Fetus ; Humans ; Pregnancy ; Principal Component Analysis ; Signal Processing, Computer-Assisted

Objective To investigate the effects and safety of mechanical continue heart chest compression ventilation (CCV) and interrupt pressure ventilation (30∶2) on cardiopulmonary resuscitation (CPR).Methods According to the mechanical pressure ventilation different way,90 patients with cardiopulmonary resuscitation (CPR) in our hospital emergency center were admitted into control group (30∶2 group) with 48 cases and observation group (CCV group) with 42 cases,with a before and after case-control study to compare the recovery effects [rate of spontaneous circulation restoration (ROCS),successful recovery rate,the ROCS time,withdraw machine time,and Glasgow coma scale(GCS) in successful recovery patients] and recovery of adveme events (fracture,skin lesions,hemopneumothorax,and visceral injury).Results With comparison between two groups,CCV group had shorter ROCS recovery time and withdraw machine time [(9.31±4.32) min vs (12.67±4.86) min,(32.07 t4.84) min vs (36.33 ± 3.37)min,P ＜0.05],higher rate of ROCS and successful recovery rate (42.9％ vs 22.9％,21.4％ vs 6.3％,P ＜ 0.05) than 30∶2 group;while both were no difference in Glasgow coma scale (GCS) in successful recovery patients (P ＞ 0.05).The incidence of adverse events were not different in both (11.9％ vs 8.3％,P ＞0.05).Conclusions Mechanical continue chest compression ventilation (CCV) compared to interrupt pressure ventilation (30∶ 2) can effectively shorten the ROCS recovery time and withdraw machine time,improve the ROSC recovery rate and success rate of recovery,but failed to improve nerve functions.Two groups have a lower incidence of adverse events,which is safe to use.

OBJECTIVE:To analyze the recent implementation and problem of GAP(Good Agricultural Practice) of Chinese traditional medicinal materials(CTMM). METHODS:The sound development of GAP in recent 10 years were given an affirmation with regard to the improvement of regulations,the progress of GAP authentication,economic achievement,scientific research and training of talents,etc.Meanwhile,the limitations in variety breeding,wildlife tending,wildlife collecting,subsequent monitoring and base organization pattern were analyzed thoroughly,and the solution was put forward. RESULTS & CONCLUSION:There has been a stable development of GAP in China; however,it remains to be improved in regulations,governmental supervising,and developmental model.

Objective To investigate the changes in the expression of Egr-1, C-jun and IL-1? mRNA and protein in the lung injured by mechanical ventilation. Methods Forty male SD rats weighing 250-320 g were randomly divided into 5 groups (n = 8 each): group A received no mechanical ventilation; group B-E received mechanical ventilaion for 30 (B), 60 (C), 90 (D) and 120 (E) minutes. The animals were anesthetized with intraperitoneal 3% pentobarbital 35 mg?kg-1 , tracheostomized and mechnically ventilated (VT =42 ml?kg-1 , RR = 40 bpm, I: E = 1:2, FiO2 = 21 % ) . Arterial blood samples were taken for blood gas analysis. The animals were killed at the end of mechanical ventilation in group B-E and after tracheostomy in group A. The lungs were removed for microscopic examination using HE staining. The expression of Egr-1, C-jun and IL-1? mRNA and protein was detected by RT-PCR and immuno-histochemical technique respectively. Results There was no significant difference in PaO2 and SaO2 among the 5 groups while PaCO2 was significantly decreased in group B and C but increased in group E as compared with group A. The expression of Egr-1, C-jun and IL-1? mRNA and protein was significantly increased by mechanical ventilation in a duration - dependent manner. Histological studies demonstrated that the damage to the lung was correlated with the duration of mechanical ventilation in terms of perivascular inflammatouy cell infiltration, exudates and hemorrhage in the alveoli and thickening of alveolar walls. Conclusion The results of our study show that mechanical ventilation activates and upregulates the expression of the early response genes in a duration - dependent manner. The upregulation of the expression of these genes might be involved in the underlying mechanism of lung damage induced by mechanical ventilation.

Objective To describe status of cancer-related fatigue (CRF) and its trajectory during three different time node for the operation, chemotherapy, and radiotherapy among breast cancer patients. Methods The convenience sampling method was used for 242 cases diagnosed with breast cancer which prepared for breast cancer resection, prepared to be treated with adjuvant chemotherapy and radiotherapy, and conformed to the sample standard from a tumor hospital and two affiliated hospital of medical college from February 2014 to November 2015 in a city. The follow-up survey of CRF was conducted respectively in the three different point-in-time for one week after the operation, at the end of chemotherapy and radiotherapy. Results After the operation mean rating for general fatigue was mild (2.59±2.41) points, and approximately 76.0% (184/242) patients experienced fatigue to low- grade degree; After the chemotherapy fatigue was significantly increased (6.57 ± 2.43) points, and 75.2% (182/242) patients accounted for severe fatigue; After the radiotherapy mean rating for fatigue was moderate (4.38 ± 2.46) points, and 57.0%(138/242) patients suffered to moderate fatigue. During the whole treatment period the level for general fatigue and four dimensions of fatigue were in the unstable state ofwaveof fluctuations with mild, severe, moderate, and fatigue were statistically different in three different stages of treatment ( F= 43.16,P=0.000). The age and operation method were associated with CRF in thephaseofoperation(age t=7.365, P=0.003; operation method t=5.166, P=0.009), chemotherapy (age t=9.835, P=0.000; operation method t=3.313, P=0.025) and radiotherapy (age t=9.014, P=0.000;operation method t=10.392, P=0.000). Conclusions There is a certain difference with CRF of breast cancer patients at different stage of rehabilitation treatment. There are two factors that could affect CRF such as age and operation method in the whole rehabilitation treatmentperiod.

A mid-aged male patient manifested hyperglycemia, severe hypokalemia, weight loss, thin skin, round face and psychoneurotic symptoms without hypertension. CT showed a pancreatic tumor and a normal pituitary image. Preliminary assessment (blood and urinary cortisol, blood ACTH and dexamethasone suppression test) supported the diagnosis of ectopic ACTH syndrome. The differential diagnosis and treatment of this disease were discussed.

Objective To explore the clinical curative effect of immunopotentiator combined with antiviral therapy in the treatment of children with recurrent respiratory tract infections.Methods 56 children with recurrent respiratory tract infections were selected as study subjects,and they were divided into two groups according to different therapeutic methods,28 cases in each group.The control group was given conventional antiviral treatment,the observation group was treated with immunopotentiator combined with antiviral therapy.The serum levels of IgA,IgG,IgM and peripheral blood T cell subsets CD3+,CD4+,CD8+ levels were detected in the two groups before treatment and after treatment.The fading away of the main symptoms and signs,and the clinical efficacy were observed.Results The fever subsided time [(1.98 ± 0.64) d],cough fade time [(1.65 ± 0.47) d] and lung's fading time [(2.38 ± 0.57) d] of the observation group were significantly shorter than those of the control group(all P ＜ 0.05).The total effective rate of the observation group was 92.86％,which was obviously higher than 67.86％ of control group(x2 =5.54,P ＜ 0.05).The serum immunoglobulin IgG [(9.09 ± 3.01) g/L],IgA [(1.75 ± 0.57) g/L],IgM [(1.39 ± 0.42) g/L] of the observation group after treatment had statistically significant differences compared with the control group (t =0.038,0.041,0.027,all P ＜0.05).The T lymphocyte subsets CD~ [(59.4 ± 8.9) ％],CD4+ [(40.5 ±4.4)％],CD8+ [(29.8 ± 4.7) ％] of the observation group after treatment had statistically significant differences compared with the control group (t =0.044,0.039,0.037,all P ＜ 0.05).Conclusion The application of immunopotentiator combined with antiviral therapy in the treatment of children with recurrent respiratory tract infections had exactly clinical curative effect,and it is worthy of popularization and application.

Neuroendocrine cells comprise a minor subpopulation of the normal prostatic epithelium. When patients accept the androgen deprivation therapy, it will induce neuroendocrine differentiation of the prostate carcinoma cells. NE cells can provide endocrine/paracrine stimuli for proliferation of the surrounding carcinoma cells and inevitably make prostate cancer become androgen independent and no longer responsive to hormonal manipulation. In this paper, the mechanism of neuroendocrine differentiation and its relationship with androgen independent state were reviewed.