Objective To investigate the application value of minimally-invasive surgical treatment in patients with traumatic epidural hematoma(EDH)straddling transverse sinus.Methods Forty-eight patients with traumatic EDH straddling transverse sinus were chosen and preoperative Glasgow Coma Scale (GCS)score ≥8 scores were managed with minimally-invasive drainage under CT location.CT scanning was performed 1 h,3 d after operation and hematoma volume was calculated,and Glasgow Outcome Scale(GOS)score was evaluated 3 months after operation.Results CT scanning showed that the hematoma volume preoperative was(46 ± 14)ml,postoperative of 1 h was(21 ± 4)ml,and 3 d was(16 ± 3)ml,decreased more obviously than preoperative,and there was significant difference(P ＜ 0.05).The GCS score preoperative was(8 ± 4)scores,postoperative of 3 d was(12 ± 2)scores,increased more obviously than preoperative,and there was significant difference(P ＜ 0.05).Three months after operation in 48 patients,GOS score 5 points 37 cases(77.1％,37/48),4 points 7 cases(14.6％,7/48),2 points 2 cases(4.2％,2/48),1 point two cases(4.2％,2/48).Conclusion Minimally-invasive drainage is effective in the treatment of traumatic EDH straddling transverse sinus with GCS score ≥ 8 scores.

Basic research and clinical research on multiple myeloma (MM) have extensively progressed, as proven by the change in the definition of complete response (CR). With improvements in laboratory technology and introduction of novel agents, CR particu-larly emphasized both micro-and macro-models. The development of CR yielded therapeutic advances in MM and vice versa. The defi-nitions of response and treatment strategies were closely connected and improved. A need exists for further detailed studies on long-term disease control, such as optimal combination of agents. Given the shortage of new drugs and the distinctiveness of health in-surance, Chinese doctors should select the best treatment projects based on real-life situation in China.

Objective To observe the variations and distribution of the pulmonary fissures of normal subjects in China on multi-planar recontruction (MPR) images of 64- slice helical CT.Methods A total of 1235 subiects underwent 64- slice helical CT scan with a slice thickness of 0.6 mm.The MPR images were observed to find normal interlobar fissures and the variations including the accessory fissures occurred in lobar or segmental lungs.Results Of the all cases,no variation was seen in 80.08%(989/1235),and pulmonary fissure variations occurred in the remaining 19.92%(246/1235).Among pulmonary fissure variations,20.33% (50 cases) occurred bilaterally,with the other 79.67%(196 cases) unilaterally.Of the unilateral pulmonary fissure variations,63.01%(155 cases) was seen in right lungs,and 16.66% (41 cases)was found in left lungs.There was neither oblique nor horizontal fissure in 1.22%(3 cases) of right lungs.Absence of oblique fissure happened more frequently in left lungs (6.91%,17 cases) than right lungs(3.65%,9 cases).Incomplete oblique fissure happened more frequently in left lungs (8.53%,21 cases)than in right lungs(4.06%,10 cases).Totally,absence of the horizontal fissures were found in 27.24%(67 cases) of the subjects,and incomplete horizontal fissures were found in 9.76% (24 cases).Accessory fissure were found in 83.33%(205 cases) of the subjects,and they happened more frequently in right lungs than in left lungs(53.66%,132 cases on the right,while 29.67%,73 cases on the left).Accessory fissures on right lower lobe and segments happened much more than on the left(40.65%,100 cases on the right,while 7.72%.19 cases on the left).Lingular fissures in the lungs were found in 15.85%(39 cases).The changes in shape and location of the oblique and horizontal fissures were found in 41.06%(101 cases),and happened more frequently in right lungs than left lungs (35.37%,87 cases on the right,5.69%,14 cases on the left).Conclusions Sixty-four-slice helical CT with MPR can clearly display pulmonary fissures and their variations,and it can distinguished small lesions from small vessels.

Objective To study diagnostic significance of spinal tuberculosis with PA and lateral view of X-ray film and CT.Methods X-ray and CT findings of 207 cases with spinal tuberculosis proved by pathology were respectively analysed.Results (1)The diagnostic rates by X-ray and CT was 93.7% and 98% respectively;(2)The diagnostic sensetivity in showing destruction of vertebral body and narrowing of interveterbral space was 74.4% and 89.9% respectively by radiography and CT;(3)The diagnostic rate only according to the destruction of vertebral body and narrowing of interveterbral space was nearly 90%;(4)Computer tomograph display of sequester formation,phamatosis in paraveterbral soft tissue,swelling of psoas muscle,involvement of bony veterbral canal,destruction of adnexa is better than the spinogram display.There is difference between two methods(?

Objective To study the feasibility and morphological characteristics of wide caliber of local biliary tract constructed by increasing pressure of local bile duct in a short period of time.Methods Twelve mongrel dogs were randomized into 2 groups.In group A,only inlaid bile duct dilator(IBDD)was placed for biliary drainage in common bile duct.In group B,IBDD was placed in tommort bile duct for biliary drainage and dilated it through affusing 0.1 ml,0.1 5 ml,0.2 ml and 0.25 ml water on postoperative day 1,8,15 and 22,respectively.The expandable ballbladders were kept on being dilated with increasing pressure for a month.Total bilirubin(TB),alanine aminotransferase (ALT),aspartate aminotransferase;aspartate transaminase(AST)of serum,bile duct diameter (BDD),histologic changes of biliary duct wall and hepatic tissue,and postoperative biliary tract contrast were determined before and after operation in both groups.Results There was no significant difference in TB,ALT,AST and histologic changes of hepatic tissue in bilary predilation and postdilation of each group and between group A and group B.There was great difference in BDD in bilary predilation and postdilation of each group and between group A and group B.In group B,the biliary wall was thickened and the lumina of bile duct widened,favorable tenacity of broaden biliary tract was shown.The specific staining of the broadened biliary wall showed that collagen and elastic fibers compacted fine and close and there was no breakage.Conclusion Dilated local bile duct constructed by increasing internal pressure of local bile duct has thick wall,wide caliber and its collagen and elastic fibers compact fine and close.This provides condition to supply early-stage cholangio-jejunostomotic reconstruction of bile duct iniury and create wide stoma for cholangio-jejunostomy in dogs.

we examined the serum copper level in 125 pregnant women (83) normal pregnant women of 28～40 weeks of pregnancy; 12 patients with se-vere edema-proteinurine-hyperterision syndromeduring 37～40 weeks of pregnancy; 30 patientswith prolonged pregnancy) and compared it withurine E/C value and Apgar score in order to deter-mine the reliability of serum copper value in diag-nosing placental insufficiency. The results con-firmed that the serum copper value was increasingwith the growth of weeks of pregnancy, but it wasdecreasing after the fortieth week of pregnancy.Comparison of the serum copper value with theurine E/C value showed that when the E/C or = 10. And when the Apgar score7. These results indicatethat the serum copper value can serve as an indexof placental function in the third trimester of preg-nancy.

Objective To study the curative effect of posterior diskoscope discectomy for adolescent lumbar disc herniation.Methods Eleven adolescent patients with lumbar disc herniation were treated with posterior diskoseope discectomy,the Japanese orthopaedic association (JOA) lower back pain score was observed and compared before operation and after operation 3,6,12 months.Results The JOA lower back pain score were (15.45 ± 2.73),(27.18 ± 2.08),(28.18 ± 1.25),(28.54 ± 1.21) scores before operation and after operation 3,6,12 months,there was statistical difference in JOA lower back pain score between before operation and after operation 3,6,12 months (P ＜ 0.01).The patients were followed up for 1-10 years,mean 7.9 years,excellent was in 8 cases,good was in 2 cases,general was in 1 case.Recurrence and iatrogenic lumbar instability were not found.Conclusions The posterior diskoscope discectomy with less trauma,less bleeding,faster recovery,and can achieve the same decompression objective with the traditional open surgical.So posterior diskoscope discectomy can be used as adolescent treatment of lumbar disc herniation.

Objective To summarize the imaging features of intra-pancreatic accessory spleen (IPAS)with multidetector computed tomography (MDCT) and improve the awareness and correct diagnosis of IPAS.Methods MDCT images of seven consecutive patients with surgically and pathologically confirmed IPAS were reviewed retrospectively.The investigated features included the location,size,shape,margin,density,and enhancement of the lesions.Results Four patients were male and three were female with a mean age of 49 years old.All the lesions were located at the dorsal side of parenchyma under the capsule of pancreatic tail.Three lesions were in round-like shape,and 4 in oval shape and all were well-defined.All the lesions were mass-like without necrosis and calcification.The maximum diameter of lesion ranged from 0.9 ～ 1.8 cm with a mean value of 1.4 cm.Compared with pancreatic parenchyma,the density of lesions were homogeneous on unenhanced CT,in arterial phase,slightly increased heterogeneous density was observed in 3 patients,slightly increased homogeneous density was observed in 4 patients.All the lesions appeared as slightly increased homogeneous density in portal phase.The CT value in unenhanced phase ranged from 50 ～ 61 Hu with a mean number of 55 Hu; and it ranged from 80 ～ 110 Hu with a mean number of 97 Hu in arterial phase; and the corresponding value was from 99 ～ 120 Hu with a mean number of 102 Hu in portal phase.Among the three patients underwent MDCT angiography,neither artery nor vein was compressed or invaded,and there was no vessel connected with lesions.Conclusions IPAS has some MDCT characteristics.For small solid mass in pancreatic tail,if the density and enhancement pattern is similar to that of spleen,the diagnosis of IPAS should be considered.

Objective To develop a method for determination of the plasma protein binding rate of bisoprolol in human plasma by high performance liquid chromatography ( HPLC) combined with hollow fiber-based liquid-phase microextraction ( HF-LPME) . Methods Method of liquid phase microextraction was optimized. The concentration of bisoprolol in the reconstitute solution was analyzed by HPLC. The mobile phase consisted of water-methanol-acetonitrile-0. 1% phosphoric acid (50:34:6:10). The excitation wavelength was 232 nm and emission wavelength was 300 nm. Through the linear regression equations, the total and free concentrations were obtained, and then the protein binding rate was calculated. Results At low, middle, and high concentration, the protein binding rate of bisoprolol was 31. 2%, 32. 0% and 31. 8%, respectively. Conclusion The proposed method is proven to be simple, fast and reproducible, and is feasible for the determination of plasma protein binding rate of bisoprolol. Bisoprolol moderately binds with plasma protein independent of concentration.

Objective To explore the role of xCT in the metastasis and drug resistance of nasopharyngeal carcinoma (NPC),to provide new evidences for the mechanism of NPC metastasis and drug resistance,and to enhance effects of chemotherapy.Methods Fluorescence quantitative polymerase chain reaction (PCR) and Western blot were used to detect the xCT expression of 5-8F and 6-10B.The xCT eukaryotic expression vector was constructed to transient transfect 6-10B cell lines.The 5-8F cell lines were treated with xCT antisense oligodeoxynucleotide (ASO).Scratching assay and transwell method were used to detect the cell metastasis and invasion abilities.The expressions of matrix metalloproteinase 1 (MMP1) of all the cell lines were surveyed by Western blot.Results The results of fluorescence quantitative PCR and Western blot showed that the expression of xCT in 5-8F was higher than 6-10B in the levels of mRNA and protein.Exogenous overexpression of xCT enhanced metastasis and invasion abilities of 6-10B cells.Silencing and inhibition of xCT could decrease metastasis and invasion abilities of 5-8F cells.The expressions of MMP1 protein in 5-8F were higher than 6-10B,and they were positively correlated with the expression of xCT.Conclusions xCT is closely related to the metastasis and invasion abilities of nasopharyngeal carcinoma.xCT could enhance the metastasis and invasion abilities of NPC cells.xCT might mediate proliferation and matastasis/invasion of NPC cells through MMP1.