1.Reflection on the Intervention of a Nursing Student with Hepatitis B Carrier and Situational Mental Disorder
Chinese Medical Ethics 1994;0(05):-
Intervention measures of a nursing student with hepatitis B carrier and situational mental disorder are reported,including inducing emotional release,helping nursing students appropriately recognize and deal with diseases,and searching effective social and family support.Related issues are also reflected on these interventions,such as to implement in-time mental nursing,stress hepatitis B related health education,deal with the conflict and balance between right of privacy and informed consent,and widespread related laws.
2.Construction and identification of lentiviral vector of RNA interference of GF?1 gene
Journal of Chongqing Medical University 1987;0(01):-
Objective: To construct a lentiviral vector of RNA interference(RNAi) of TGF-?1 gene. Methods: Three target sequences were selected according to TGF?1 mRNA sequence firstly,then three pairs of oligonucleotide sequences according to these target sequences and one pair of negative control oligonucleotide sequence were designed and synthesized. The annealed oligonucleotide fragments were subcloned into pRNA-U6/Lenti plasmid. After identified by restriction enzyme digestion and sequencing ,these plasmids were cotransfected along with ViraPowerTM Lentiviral Packaging Mix into 293FT cells for the package of lentiviral particles. Then the lentiviral vector particles were transfected into Hela cells and TGF-?1 expression in the transfected cells was assayed by Real-Time PCR and ELISA. Results: It was confirmed by digestion and sequencing that lentiviral vectors had the correct structure and could express high titer of virus. After transfected into Hela cells,TGF-?1 expression was decreased significantly by two of these lentiviral vectors at both the protein and mRNA level and the first vector had the best interefence efficiency. Conclusion: The lentivirus RNAi vector of TGF-?1 was constructed successfully.
3.The value of the combine detection of blood lipid,blood glucose,liver function and serum uric acid in the diagnosis of elderly patients with fatty liver
International Journal of Laboratory Medicine 2017;38(15):2040-2042,2045
Objective To explore the application value of blood lipid,blood glucose,liver function and serum uric acid in the diagnosis of fatty liver in elderly patients.Methods A total of 62 elderly patients with fatty liver in our hospital from April 2016 to October 2016 were selected as study group,and 62 healthy persons were selected as control group.All the subjects were drawn fasting venous blood 4 mL.Rate method was used to detected liver function indexes of total bile acid(TBA),gamma glutamyltranspeptidase(GGT) and aspartate aminotransferase(AST),alanine aminotransferase(ALT),total bilirubin(TBIL) levels.The hexokinase method was used to detected blood lipid indexes,including very low density lipoprotein cholesterol(VLDL-C),low density lipoprotein cholesterol(LDL-C),high density lipoprotein cholesterol(HDL-C),cholesterol(TC),triglyceride(TG) levels.The peroxidase colorimetric method was used to detected blood glucose index including glycated hemoglobin(HbA1c) and fasting blood glucose(FBG) levels.MINDRAY BS-400 automatic biochemical analyzer produced in Shenzhen MINDRAY company was used to detect the serum uric acid level.Value of blood lipid,blood glucose,liver function,serum uric acid in diagnosis for fatty liver were made statistical comparison.Results The levels of TBA,GGT,AST,ALT and TBIL in the study group were higher than those in the control group,the level of VLDL-C,LDL-C,TG,TC in the study group were higher than those in the control group,the HDL-C level was lower than that in the control group,the levels of FBG and HbA1c in the study group were higher than those in the control group,the level of serum uric acid in the study group[(447.12±91.03)μmol/L] was higher than that in the control group [(353.41±70.32)μmol/L],the differences were all had statistically significant(P<0.05).The sensitivity(80.65%,79.03%,82.26%,77.42%) and accuracy(90.32%,88.71%,90.32%,88.71%) of blood lipids,blood glucose,liver function,blood uric acid alone in diagnosis of fatty liver were lower than the combined diagnosis(95.16%,96.77%),the differences were statistically significant(P<0.05).Conclusion Fatty liver patients have abnormal blood lipids and blood glucose,liver function and blood uric acid level,combined with the above indexes for diagnosis can improve the sensitivity and accuracy of fatty liver,and provide guidance for the intervention,is worthy of promotion.
5.Factors associated with length of hospitalization in patients admitted with transient acute transverse myelitis
Clinical Medicine of China 2015;31(12):1096-1098
Objective To evaluate the days of hospitalization of acute transverse myelitis and its influencing factors.Methods File's of 158 patients with acute transverse myelitis as the first diagnosis in Feicheng Mining Centre Hospital were analyzed retrospectively.Access to the original medical records through the medical record number, and supplemented information, mainly including demographic data, neurological function defect degree, complications and complications.Results Hospitalization time was 4-41 d, an average of (17±6) d,including 23 patients hospitalized time ≤ 10 d(14.56%), 83 cases were > 10-<20 d(52.53%), 52 cases was≥20 d(32.91%).Age and Neurological score comparative differences are statistically significant (F=60.90,8.68, P<0.05).Payment method, deep vein thrombosis, comparative differences were statistically significant(x2 =20.04, 14.27, 12.17,9.37,7.05;P<0.05).There were ralationship between acute transverse myelitis with age, neurological score, payment (OR =1.140, 0.500,83.930,0.041;P< 0.005).Conclusion The degree of neurologic impairment is the most important influencing factors of acute transverse myelitis.Controlling hospital infection, preventing deep vein thrombosis, strengthening medical insurance management, are the effective ways to shorten the days of hospitalization of acute transverse myelitis.
6.Problems and Counter - measures of Protection on Rights of Patients'Privacy in Nursing Practice
Chinese Medical Ethics 1994;0(06):-
To reduce and avoid the contradiction caused by the right of privacy in nursing practice, the author analyzed problems of patients'privacy in nursing: distance between nursing staffs cognition and practice; doctors' opinion is different from nurses'; the contradiction between privacy and informed consent; problems of privacy relate to bed side teaching; new contents brougth out by science development. Aiming at the problems, the author puts forward corresponding countermeasures: to enhance the study of law; to balance the right to privacy and informed consent; to respect patients'privacy, strengthen communication with patients; and to understand new contents about ethics.
7.Determination of Icariine in Qianlietong Tablets by HPLC
China Pharmacy 2005;0(21):-
OBJECTIVE:To establish an HPLC method for the determination of Icariine in Qianlietong tablets.METHODS:Hypersil ODS(200 mm?4.6 mm,5 ?m) column was used for the HPLC determination,and the mobile phase consisted of acetonitrile-water(30∶70) at a flow rate of 1.0 mL?min-1.The detection wavelength was 270 nm.RESULTS:Icariine has good linear relationship in the range of 0.050 6~0.506 0 ?g(r=0.999 9).The average recovery was 102.67%(RSD=0.99%,n=6).CONCLUSION:The established method is simple,reproducible,specific and suitable for the quality control of Qianlietong tablets.
8.Research Progress in Ethical Issues of Intra-vital Organ Transplantation
Chinese Medical Ethics 1994;0(06):-
Intra-vital organ transplantation is an advanced medical technique for the treatment of patients with terminal stage organ failures.However,related ethical issues arise with the development and application of the technique.Some research has been done on relevant ethical issues in intra-vital organ transplantation with certain ethical principles and regulations arisen,which are discussed in this article.
9.Ethical Analysis of All-Free Health Care in Shenmu County
Chinese Medical Ethics 1996;0(01):-
The "all-free health care" system is being implemented in Shenmu County in Shaanxi Province,whose ethical orientation is during the transitional process from "basic medical insurance" to "meeting residents′ actual healthcare demand".The "all-free health care" system safeguards human health rights,embodies the principle of giving priority to social benefits,and is in accordance with the principle of effectiveness.However,due to the lack of scientific management,the capital flows violate the principle of prevention in the system.
10.Effect of Shiwei Longdanhua particles on serum inflammatory factors and immune function in elderly patients with acute bronchitis
Chinese Journal of Biochemical Pharmaceutics 2017;37(3):98-100
Objective To observe the effect on serum inflammatory factors and immune function of Shiwei Longdanhua particles in elderly patients with acute bronchitis.Methods 120 cases of elderly patients with acute bronchitis from November 2014 to October 2016 were selected and randomly divided into the observation group and the control group with 60 cases in each group, according the order of single and double number.The control group was given conventional treatment, and the observation group was given Shiwei Longdanhua particles based on the control group.Treatment for seven days, to observe the differences of serum inflammatory factors (hs-CRP, TNF-αand IL-6, IL-1) and immunologic function indexes (CD3 +, CD4 +, CD8 +, CD4 +/CD8 +and IgM, IgG, IgA) between the two groups.Results After treatment, hs-CRP, TNF-αand IL-6, IL-1 of the two groups decreased with before treatment, the difference was statistically significant (P<0.05), and all indexes of the observation group were lower than those of the control group, the difference was statistically significant ( P<0.05 ) .After treatment, CD3 +, CD4 +, CD4 +/CD8 + of the two groups increased with before treatment except CD8 +decreased, the difference was statistically significant (P<0.05), there was no significant difference between the control group and before treatment, there was significant difference between the two groups after treatment (P<0.05).After treatment, IgM, IgG and IgA of the two groups decreased with before treatment, the difference was statistically significant (P<0.05), and all indexes of the observation group were lower than those of the control group, with significant differences, the difference was statistically significant (P<0.05).Conclusion Shiwei Longdanhua particles has the effects of inhibiting inflammatory reaction and improving the immune status in the treatment of elderly patients with acute bronchitis .