The PTC hot air drying device controlled by a single-chip computer has such advantages as rapid heating, automatic isothermal, weak leakage current and being time-proof, which applies to all calibers of pipeline.

Objective:To investigate the effect of telmisartan and fosinopril on the expression of angiotensin-convertion enzyms 2 (ACE2) in cardiomyocyte after myocardial infarction.Methods:Animal model of acute myocardial infarction were established by ligating the left anterior descending coronary artery of rats. Vehicle, telmisartan,fosinopril,or both drugs combined were giv- en to rats by intubation feeding,from 2 weeks before coronary artery ligation to 2 weeks there after. ACE2 protein expression of myocardium were semi-quantified by SP immunohistochemistry. ACE2mRNA were measured by Reverse Transcriptase Poly- merase Chain Reaction (RT-RCR). Results:Compare with sham operated group,ACE2 protein increased significantly in infarc- tion group,but not the ACE2 mRNA. telmisartan and fosinopril therapy caused a significant increase in ACE2 protein compare to infarction group,but only telmisartan caused a significant increase in ACE2 mRNA though all therapied groups showed higher ACE2 mRNA expression. Conclusion:Both telmisartan and fosinopril induced increases in cardiac ACE2 exprssion, whereas the combination of these two drugs do not associated with higher ACE2 expression.

Objective To develop a secure network system to monitor the temperatures in baby incubators.Methods By utilizing patented TAV temperature monitoring system,the running status of temperature-controlled equipments was recorded in digital way,and then it was analyzed and managed dynamically;and real time alarms could be given through networks.Results This system is a kind of security devices independent of baby incubators and can monitor temperatures in baby incubators well.Conclusion This system will not affect original functions of baby incubators,instead it can well monitor the temperatures in both imported and domestic incubators,so that it can improve the safety of facilities being used while decrease medical accidents.

Antibody affinity influences the ant igen-antibody reaction in vivo and in vitro. It is important to routinely determine the antibody affinity in order to elucidate the impacts of this antibody characteristics on the immunoassay and the pathogenetic mechanisms of human diseases. In this paper, a simple method (modified Friguet' s) was reported to directly measure the experimental affinity data of monoclonal antibody in ascites by means of ELISA and to accurately determine the corresponding affinity constants of monoclonal antibody by amendment of underestimation of affinity constants due to the intrinsic properties of Friguet' s, and shows convenience and reliability.

Objective To balance and optimize four key control parameters, acid-base pH, viral spiking(MOI), TPCK trypsin spiking and cell density in MDCK cell suspension culture of influenza virus H3N2, in order to improve the productivity and virus titer of influenza virus H3N2 in MDCK cell suspension culture.Methods A multi-factorial interaction experimental design was created using MODDE~?software of Design of Experiment(DoE) from Sartorius to determine and optimize the infectivity titer of H3N2 virus, considering the interactive effects of four factors: pH, MOI, TPCK trypsin concentration and cell density, aiming to achieve an optimal infectivity titer within a certain range of these four factors.Results The optimal ranges of MDCK cell suspension culture conditions for H3N2 virus were as follows: pH range of 7. 88-8. 00, MOI of 0. 001,TPCK trypsin concentration range of 1. 70 to 6. 0 μg/mL, and cell density range of(55. 70-77. 28 × 10~5) cells/mL. The virus was maintained in Xene-S001 medium at a cultivation temperature of 34. 5 ℃ for 72 h, with a shaking speed of 100 r/min and a CO_2 concentration of 3%. Under these conditions, the TCID_(50) of H3N2 reached 6. 5, and the CV of repeated experiments for TCID_(50) was 1. 03%, with good reproducibility and stability.Conclusion H3N2 virus in MDCK cell suspension culture can achieve higher virus titer.

Several researches have suggested that estrogen contributes to the initiation and development of thyroid cancer by binding to estrogen receptors (estrogen receptor α,estrogen receptor β,and G-protein-coupled receptor 30),activating gene or non-genomic pathways and regulating proliferation of thyroid cells.Studies on antiestrogen drugs based on inhibiting thyroid cell proliferation may provide a new target in treating thyroid cancer.

Child ; Child, Preschool ; Epilepsy, Generalized ; complications ; epidemiology ; genetics ; Female ; Humans ; Infant ; Male ; Pedigree ; Phenotype ; Seizures, Febrile ; complications ; epidemiology ; genetics

Apoptosis ; Fatty Liver, Alcoholic ; Humans ; Lipid Metabolism

BACKGROUND: The posterior circulation artery is characterized by small caliber, long traveling, many curves and branches, so a stronger resistance will be encountered when a stent is delivered.OBJECTIVE: To investigate the effects of coaxial double catheter technique on intracranial posterior circulation stenting.DESIGN, TIME AND SETTING: A retrospective case analysis was performed at the Department of Neurology, Affiliated People's Hospital of Yunyang Medical College between December 2004 and February 2008.PARTICIPANTS: A total of 21 patients, 11 males and 10 females, aged 50-76 years old, were se/ected from the Department of Neurology, Affiliated People's Hospital of Yunyang Medical College.METHODS: All patients were confirmed as posterior circulation intracranial stenosis (stenosis > 70%) by digital subtraction angiography (DSA), magnetic resonance angiography (MRA), or CT angiography (CTA). They underwent cerebrovascular stent implantation, during which, single catheter technique was first applied, if failed, a coaxial double catheter technique was followed. A conventional group (single catheter technique successfully applied) and a double catheter group (coaxial double catheter technique successfully employed) were set. A written informed consent was obtained from each patient.MAIN OUTCOME MEASURES: Therapeutic results and biocompatibility of implanted stents.RESULTS: The single catheter technique was applied in 21 patients and 6 of them (28.57%) had successful stent deployment, The remaining 15 patients were managed by a coaxial double catheter technique, and the stent was successfully released in these patients. There was only 1 patient presenting with subarachnoid hemorrhage, and the success rate of stent deployment was 93.33%. Chi-square tests results showed that the coaxial double catheter technique produced significantly higher success rate of stent implantation than the single catheter technique (P < 0.05). Except this, no other adverse reactions were found. All stents covered the stenosis lesions completely, and fitted well with the blood vessel wall without rejection, showing the good biocompatibility of selected stents with the host.CONCLUSION: The coaxial double catheter technique can increase the longitudinal supporting strength and enhance the success rate of the intracranial posterior circulation stenting. Meanwhile, the risks of this procedure are low. Therefore, the technique deserves to be popularized.

Objective To investigate the effect and mechanism of Src kinase in renal interstitial fibrosis of unilateral ureteral obstruction (UUO) mice.Methods Male C57BL/6J mice were randomly divided into 4 groups,including sham operation group (n=8),sham operation+PP2 group (n=8),UUO operation group (n=8) and UUO operation+PP2 group (n=8).The mice were injected 2 mg/kg PP2 by intraperitoneal everyday after surgery in sham+PP2 group and UUO+PP2 group.PP2 dissolved in 1％ DMSO (formulated with normal saline).Sham and UUO group were given equal 1％ DMSO.The mice were sacrificed at 7th day.Renal collagen was observed with Sirius red stain.The activities of Src,protein kinase B (PKB,AKT),p38 mitogen-activated protein kinase (p38 MAPK),extracellular signal-regulated kinase (ERK) and the protein expressions of α-smooth muscle actin (α-SMA) and fibronectin (FN) were detected by Western blotting.The expression of collagen I (COL [) was detected by immunohistochemistry and the expressions of matrix metalloprotein 9 (MMP-9),tissue inhibitor of metalloproteinase 1 (TIMP-1),transforming growth factor-β31 (TGF-β31),monocyte chemotactic protein-1(MCP-1),interleukin-6 (IL-6) were measured by ELISA.Resuts Compared with sham mice,UUO mice on 7th day displayed obvious renal fibrosis.Meanwhile,UUO mice had increased expressions of COL Ⅰ and FN,and activities of AKT,ERK and p38 MAPK (all P ＜ 0.05).Their renal expressions of α-SMA,TGF-β1,MMP-9,TIMP-1,MCP-1 and IL-6 were also raised (all P ＜ 0.05).Compared with those in UUO group,in UUO + PP2 group the activities of Src,AKT,p38 MAPK and ERK,and expressions of TGF-β1,MCP-1 and IL-6 decreased (all P ＜ 0.05).Additionally,expressions of COL Ⅰ,FN and α-SMA,collagen deposition and renal fibrosis receded in UUO + PP2 group (all P ＜ 0.05).However,the expressions of MMP-9 and TIMP-1 were not influenced by PP2 treatment.Conclusions Src kinase promotes myofibroblasts accumulation and inflammatory reaction through activating its downstream signaling pathway in the progressing of renal interstitial fibrosis.