AIM: To prepare controlled release tablets of colchicine and establish the relationship among formula factors of membrane and drug release rate. METHODS: Uniform design was used to achieve the quantitative relationship among the release rate of colchicine,acetyl cellulose and PEG 6000.Optimized prescriptions were calculated from the regression equation. RESULTS: A significant correlation and a fine precision were obtained.(Osmotically) controlled release tablets of colchicine were prepared based on the calculations,and the drug release pattern was in accordance with zero-order kinetics(r=0.990 2). CONCLUSION: Uniform design is a convenient and efficient approach to the formulation optimization of elementary osmotic-pump tablets.

AIM: To investigate the optimum core formulation and drug release profile of colchicine osmotic pump tablets. METHODS: The key influencing factors and optimum core formulation were studied based on the cumulative drug release rate at different timepoints and homogenous factor(f_2) in combination with the orthoganal design. RESULTS: The drug release profile in vitro was influenced by components of the core tablet,including(amount) of osmogent,solubilizer and adherent.The optimum in vitro drug release profile obeyed the zero-order release model within 1～10 h(r=0.990 2),and drug released at 12h reached up to 85%. CONCLUSION: The colchicine osmotic-pump tablets are attainable by constant speed delivery.

Objective: To detect the ability of medicine serum of Changantai and its major components to kill Co26lu colon cancer cells in vitro by means of serum pharmacology.Methods: There were 5 groups of rats.5-Fu 30mg/kg were injected into the abdominal cavity of the rats in 5-Fu blood serum group and abdominal aorta blood was drawn 1 hour after administration.The other four groups were intragastrically administrated with Changantai(dry paste),rhubarb(dry paste),radix actinidiae(dry paste) and NS 0.6ml/kg/d respectively and the blood was draw twice,that was,2ml orbital vein hemospasia 1 hour after administration and abdominal aorta hemospasia 1 hour after the last administration of three consecutive days with asceptic serum segregation.Ability of medicine serum to kill tumor cells was observed by means of MTT method.Results: medicine serum of 5-Fu,Changantai and its components with different concentration had different ability in vitro to kill Co26lu cells,which was concentration dependent.Conclusion: Changantai can inhibit cancer cells from growing and metastasis.

This paper summarizes the new progress of the research of the targeted microbubble ultrasound contrast agents and ultrasound molecular imaging in tumor angiogenesis.There are two constructing methods of targeted microbubble ultrasound contrast agents.Targeted new blood vessels microbubble unltrasound contrast agents are mainly divided into the connection of alpha(V)beta3 antibody,αVβ3 integration antibody and connecting VEGFR2.The quantitative analysis of targeted microbubble adhering to tumor target and the evaluation of the correlation between targeted microbubble adhering to tumor neovascular endothelial cell and tumor local pathological microvessel density suggest that ultrasound molecular imaging can be noninvasive,quick,repeated,dynamic and quantitative evaluate tumor angiogenesis and indirectly reflect the malignant tumor tissue proliferation activity and its biological characteristics.

Objective To compare the ability of auditory verbal learning,memory and retention between each side of thalamus and basal ganglia after stroke.Methods 63 patients with single lesion in thalamus or basal ganglia after stroke,34 patients with amnestic mild cognitive impairment (aMCI)and 34 healthy control subjects received auditory verbal learning test.Results There was an overall decline of immediate and delayed recall,retention ability in patients with single lesion of stroke as compared with the healthy control group(P＜0.05).Both the left thalamic stroke group [(19.0± 3.1)scores]and aMCI group[(17.6 ±3.3)scores]showed similar pattern in damaged recognition ability,while this ability still existed in the other three stroke groups.The ability of auditory verbal learning in the left thalamic stroke group[(2.2 ± 2.0)scores]and right thalamic stroke group[(2.1 ± 1.9)scores]were lower than in the healthy control group[(3.6 ± 1.8)scores](P ＜0.05).The retention ability in the left thalamus stroke group[(2.8±1.7)scores]and the right basal ganglia stroke group[(2.7 ± 1.9)scores]was decreased than in the healthy control group[(1.7±1.4)scores](P＜0.05).Conclusions There is decline of AVLT-learning,memory and retention ability in patients with single lesion stroke,especially in the left thalamus stroke patients.Moreover,the pattern of impairment in the left thalamus stroke is similar to aMCI but different from the other three stroke groups.

Ten patients with 12 inflammatory pseudotumor of the liver (IPL) lesions were routinely examined by gray scale uhrasonography(B-US),color Doppler (CD)and pulscd Doppler(PD). All of them in B-US were hypoechoic pattern with inhomogeneity,and one of them displayed helo-like,margin, suggesting malignant tumor. However, the irregular shape with calabash-like was found in 10 IPL lesions by B-US, being different from liver cancer.The color blood flow and arterial spectrum were detected by CD and PD in the margin 3 of 9 IPL lesions,and the resistant index was less than 0.5 in 2 of 3 IPL with Doppler signals, 6 of them were suggested to lack of blood supply, the manifistations of Doppler were consistent with pathology of IPL. Therefore, 8 lesions were considered to be benign lesions by Doppler US and the accuracy for the diagnosis of IPL was 56%(5/9)by using Doppler combined with B-US.

This article was aimed to analyze literatures on preventive treatment of disease in acupuncture and moxi-bustion in order to understand its status and development trend. Bibliometrics and statistics method in NoteExpress were used in the analysis of related literatures about preventive treatment of disease in acupuncture and moxibustion from 1981 to 2013, which were included in CNKI. The analysis and research were from aspects such as year distri-bution, keyword, journal distribution, author distribution, and fund distribution. The results showed that there were 537 articles in total from 1981 to 2013. The number of literatures was in an overall growing trend.“Zusanli”,“Inner Canon of Huangdi”,“sub-health”, and“clinical research”were high frequency keywords. The journal distribu-tion conformed to the Brad Ford’s law. The author distribution conformed to the Lotka’s law. The number of fund papers was also increased. It was concluded that research on preventive treatment of disease in acupuncture and moxibustion was still in the period of rapid development and had not reached maturity. The preventive treatment of disease in acupuncture and moxibustion had broad application prospects. However, the research still required to be strengthened.

OBJECTIVE To study the prevalence,pathogenic characteristics and drug resistance of the invasive fungal infection to provide the reference for clinical treatment.METHODS The fungi were cultured and identified conventionally.The susceptibility test was performed by K-B disk diffusion methods.The data were analyzed by WHONET 5.3 software.RESULTS Among 232 strains of fungi,isolating rate of fungi from sputum was the highest,accounting for 74.1%.The following was urine,accounting for 17.2%.The most common organisms were Candida(96.6%)among which white Candida albicans accounted for 58.6%,following by C.tropicalis(13.8%),C.parapsilosis(10.3%),C.glablate(8.6%)and C.krusei(4.3%).Drug resistance to four commonly used antifungal drugs showed drug sensitivity to amphotericin B and itraconazole was higher,while that to fluconazole and flucytosine was lower.CONCLUSIONS Significant difference of drug resistance occurs among invasive fungus species.The cross resistance to azole antifungals is existing and has a raising tendency.

Usage of the fruit and bark of a Melia-family plant as a digestive tract-parasiticide and agricultural insecticide was recorded about two thousant years ago in ancient China. Toosendanin (TSN), a triterpenoid, is an effectual ingredient extracted from the plant. Studies have demonstrated that TSN selectively affects neurotransmitter release, effectively antagonizes botulism, induces cell differentiation and apoptosis and inhibits proliferation of various human cancer cells, inhibits feeding and dovelopment in insects and modifies K+- and Ca2+-channel activity. The research data to demonstrate that TSN inhibits K+-channel and facilitates L-type Ca2+-channel are summarized, and the mechanism of action of TSN is discussed.