Aged ; Angiofibroma ; metabolism ; pathology ; Desmin ; metabolism ; Diagnosis, Differential ; Female ; Humans ; Inflammation ; Leiomyosarcoma ; pathology ; Neoplasms, Muscle Tissue ; metabolism ; pathology ; Vimentin ; metabolism ; Vulvar Neoplasms ; metabolism ; pathology

OBJECTVEThis study evaluates the expression of interleukin-18 (IL-18) and hypoxia-inducible factor (HIF)-lα in rat periodontitis model exposed to normoxia and chronic intermittent hypoxia (CIH) environments. The possible correlation between periodontitis and obstructive sleep apnea-hypopnea syndrome (OSAHS) was also investigated. Methods: Thirty-two Sprague-Dawley (SD) rats were randomly assigned into four groups: normoxia control, normoxia periodontitis, hypoxia control, and hypoxia periodontitis groups. The periodontitis models were established by ligating the bilateral maxillary second molars and employing high-carbohydrate diets. Rats in hypoxia control and hypoxia periodontitis groups were exposed to CIH treatment mimicking a moderately severe OSAHS condition. All animals were sacrificed after eight weeks, and the clinical periodontal indexes were detected. The levels of IL-18 and HIF-1α in serum and gingival tissues were determined using enzyme-linked immunosorbent assay (ELISA). The correlation between attachment loss (AL) and the levels of IL-18 and HIF-lα in hypoxia periodontitis group was evaluated.

RESULTSThe levels of IL-18 and HIF-lα in hypoxia periodontitis group were significantly higher than that in normoxia periodontitis and hypoxia control groups (P<0.05). Furthermore, the levels of IL-18 and HIF-lα in serum (r-0.792, r=0.753, P<0.05) and gingival tissues (r-0.817, r=0.779, P<0.05) were positively correlated with AL.

CONCLUSIONCIH could aggravate the destruction of periodontal tissues, which is correlated with IL-18 and HIF-lα levels.

Animals ; Disease Models, Animal ; Enzyme-Linked Immunosorbent Assay ; Gingiva ; Hypoxia ; Hypoxia-Inducible Factor 1, alpha Subunit ; Interleukin-18 ; Periodontal Index ; Periodontitis ; Rats ; Rats, Sprague-Dawley ; Serum ; Sleep Apnea, Obstructive

Adult ; Aged ; Ascitic Fluid ; chemistry ; Blood Gas Analysis ; methods ; Carbon Dioxide ; analysis ; blood ; Female ; Humans ; Hydrogen-Ion Concentration ; Liver Cirrhosis ; blood ; Male ; Middle Aged ; Oxygen ; analysis ; blood

When people are walking, they will produce gait signals and different people will produce different gait signals. The research of the gait signal complexity is really of great significance for medicine. By calculating people's gait signal complexity, we can assess a person's health status and thus timely detect and diagnose diseases. In this study, the Jensen-Shannon divergence (JSD), the method of complexity analysis, was used to calculate the complexity of gait signal in the healthy elderly, healthy young people and patients with Parkinson's disease. Then we detected the experimental data by variance detection. The results showed that the difference among the complexity of the three gait signals was great. Through this research, we have got gait signal complexity range of patients with Parkinson's disease, the healthy elderly and healthy young people, respectively, which would provide an important basis for clinical diagnosis.
Aged ; Gait ; Healthy Volunteers ; Humans ; Parkinson Disease ; physiopathology ; Reference Values ; Signal Processing, Computer-Assisted ; Walking

The quality of sleep has a great relationship with health and working efficiency. The result of sleep stage classification is an important indicator to measure the quality of sleep, and it is also an important way to diagnose and treat sleep disorders. In this paper, the method of detrended cross-correlation analysis (DCCA) was used to analyze sleep stage classification, sleep electroencephalograph signals, which were extracted from the MIT-BIH Polysomno graphic Database randomly. The results showed that the average DCCA exponent of the awake period is smaller than that of the first stage of non-rapid eye movement (NREM) sleeps. It is well concluded that the method of studying the sleep electroencephalograph with this method is of great significance to improve the quality of sleep, to diagnose and to treat sleep disorders.
Electroencephalography ; Humans ; Signal Processing, Computer-Assisted ; Sleep Stages

Microbial genome reduction and modification are important strategies for constructing cellular chassis used for synthetic biology. This article summarized the essential genes and the methods to identify them in microorganisms, compared various strategies for microbial genome reduction, and analyzed the characteristics of some microorganisms with the minimized genome. This review shows the important role of genome reduction in constructing cellular chassis.
Genes, Essential ; genetics ; Genetic Engineering ; Genome, Microbial ; genetics ; Synthetic Biology ; methods

Personalized therapy has became a main method to break through efficacy of chemotherapy in lung cancer. At present, we have got a few progressions in personalized therapy, but which are very limited. This review discourses upon the dilemma and hope of individualized treatment from the following ways:limitation of based on histology type to determine treatment regimens, undecided sequential therapy after failure of TKI as front-line therapy for advanced NSCLC patients with EGFR mutation, clinical study of maintenance therapy and EGFR mutation detection based on peripheral blood being trap or surrogate for tumor tissue to direct personalized TKI therapy.

RNA interference (RNAi) is a post-transcriptional suppression method of introduction of double-stranded RNA molecules by artificially induced. Recently RNAi has been widely used in xenografted tumors of nude mice in vivo. In order to inhibit the growth of tumor, some genes related with tumor such as anti -apoptosis gene, proliferation promoting gene, metastasis-associated gene are depressed via RNAi. Additionally, in the nude mice test, RNAi is utilized as assisting methed of radiotherapy and chemotherapy of human tumors.

Objective To investigate the fasting plasma insulin (FINS) and free fatty acid (FFA) in overweight or obesity patients with type 2 diabetes mellitns (T2DM) Methods 263 patients of T2DM were selected and divided into normal weight group(n = 108) and overweight or obesity group(n = 155) according to body mass index (BMI) , FINS level was detected by chemiluminescence method , and FFA level by colorimetry. 72 subjects without T2DM were taken as controls. Results FINS [(6.18±1.86) mIU/L vs (4.60±2.58) mIU/L, P < 0.01)] and FFA [(0.58±0.25) mmoL/L vs (0.35±0.14) mmoL/L,P < 0.01)] in T2DM group was significandy higher than that of control group. FINS in overweight or obesity group [(7.20±1.99) mIU/L] was higher than that in normal weight group[(5.31±1.21) mIU/L] and control group[(4.60±2.58) mIU/L] (P <0.01). In T2DM group,FiNS was positively correlated with duration(r =0. 334,P <0.01) ,BMI(r =0. 532,P <0. 01) and diastolic pressure (DBP) [r =0. 342 ,P <0.01). Conclusion The FINS level in overweight or obesity patients with T2DM is significantly higher compared to T2DM with normal weight T2DM,and the FFA level in patients of T2DM is significantly higher increased compared to control group,both of which participate in insulin resistence as well as the occurrence and development of T2DM.

Objective To analyze the curative effect of acupoint catgut-embedding therapy for PCOS patients of obesity.Methods 60 PCOS patients are randomly divided into an experimental group (acupoint catgut embedding group)and a control group (acupuncture group),with 30 cases in each group.Observe such indexes of each patient as weight,lumbar,BMI and menses before and after treatment.Results The two methods are significantly effective to reduce weight and improve the menstrual disorder,with no statistically significant difference(P＞0.05).Conclusion Acupoint catgut-embedding therapy is an effective method to treat PCOS of obesity.