Objecctive To study the expression and significance of CD44V6 and E-Cd in gallbladder carcinoma. Methods The expression of CD44V6 and E-Cd was detected by immunohistochemical technique in 48 cases of gallbladder carcinoma, 29 cases of gallbladder adenomal polypi and 25 cases of chronic calculous cholecytitis. The association of CD44V6 and E-Cd expression with the clinical and pathologic parameters was analyzed. Results E-Cd expressed in 21 of 48 cases of gallbladder carcinoma(43 75%), 21 of 29 cases of gallbladder adenomal polypi(72 42%) and 24 of 25 cases of chronic calculous cholecytitis(96 00%), while CD44V6 expressed in 31 of 48 cases of gallbladder carcinoma(64 58%), 9 of 29 cases of gallbladder adenomal polypi(31 03%) and 1 of 25 cases of chronic calculous cholecytitis(4 00%). The expression rates of CD44V6 and E-Cd in the three kinds of tissues were significantly different(P

OBJECTIVE:To promote the sound development of clinical pharmacy.METHODS:The practice and signifi?cance of the clinical pharmacy of our hospital were analyzed and evaluated so as to put forward methods for further development of clinical pharmacy.RESULTS&CONCLUSION:Providing the clinical pharmaceutical services is the general trends and the prospect for which is promising.

Hypcrlipidemia is one of etiological factors of pancreatitis,of which the genesis has somewhat speci ficity.This article reviews hyperlipidemia related pancreatitis about its conception,etiology,classification,pathogene sis.specific clinical manifestation as well as diagnosis and treatment.

The spectrum of glomerulonephritis differs in different countries and areas all over the world,and changes with socioeconomic progression. Recent researches have shown that the difference and change of the spectrum of glomerulonephritis are correlated with Th1 and Th2 immune responses."Hygiene hypothesis" is also proposed.

[Objective] To improve the way of establishing myocardial ischemia reperfusion rat model.[Methods]30 SD rats,quality(220?20)g,male,randomly split into normal model group and improved model group,improved established model ligation with a 0.6mm diameter line,and drawn after 30min.Compare such a method with normal model.[Results] In the context of keeping experimental stability,the reduced heart rate,wake time and survival rate of the way of improved established model respectively are(50?20)/min,(15?5)min,86.67%,but it is just(120?20)/min,(80?20)min,66.67% of the normal model group.[Conclusion]The improved myocardial ischemia reperfusion model offers significant superiority to the former one.

AIM:To study effects of resveratrol on osteoprotegerin(OPG)and receptor activator of NF-?B ligand(RANKL)expression in femur of ovariectomized rats.METHODS:48 female SD rats(3 months old)were assigned into 6 groups randomly:sham-operated group(SHAM);ovariectomized group(OVX);17?-estradiol replacement therapy group(ERT,0.1 mg?kg-1?d-1,sc);high-dose resveratrol-treate group(RH,40 mg?kg-1?d-1,i.p.);middle-dose resveratrol-treated group(RM,20 mg?kg-1?d-1,i.p.);low-dose resveratrol-treated group(RL,10 mg?kg-1?d-1,i.p.).Except for SHAM group,rats in other groups were operated by bilateral ovariectomy.Administration started at the 8th day after operation and lasted 8 weeks.At the end of the 9th week after operation,all rats were sacrificed.The bone mineral density(BMD)and vitodynamics properties(including ELASTIC,M-STRESS,M-LORD and STIFFNESS)of femurs were measured.The expression of OPG and RANKL in femurs of rats were observed by immunohistochemical staining methods.RESULTS:Compared with OVX group,resveratrol treatment(40 mg?kg-1?d-1 and 20 mg?kg-1?d-1)increased expression of OPG in femurs while decreased expression of RANKL in ovariectmized rats.In RH,RM,and ERT group,BMD,ELASTIC,M-STRESS of femurs were higher than those of OVX group.CONCLUSION:Resveratrol increased expression of OPG in femur while decreased expression of RANKL in ovariectmized rats,which was probably one of mechanism that resveratrol improve osteoporosis.

Objective To explore the curative effect of dissection steel plate treatment in humerus near end bone fracture curative effect.Methods From June 2000 to March 2006,dissection steel plate treatment was used in 31 cases of humerus near end bone fracture and Constant-Murley scores was used to evaluate the curative effect.Results All patients were made a follow-up for 10 to 24 months,the average 16.5 months.31 examples surgeries patient only superior 16 examples,good 10 examples,may 2 examples,difficult 3 examples.Conclusion Center 3 and 4 humeruses near ends bone fracture uses the dissection steel plate treatment to Neer dividing will be possible to obtain the good clinical effect.

Objective To analyze the factors influencing the total cost of hospitalization after internal fixation of intertrochanteric fracture.Methods Enrolled in this study were 142 patients with intertrochanteric fracture who had been treated at our department between March 2013 and August 2014.They were 54 men and 88 women,aged from 24 to 95 years (average 69.8 years).Intramedullary fixation was adopted in 135 cases while extramedullary fixation in 7.By AO classification,32 cases were type A 1,75 type A2 and 35 type A3.We analyzed their demographic data,fracture-associated factors,bleeding-associated factors and surgery-associated factors.For univariate analysis,the independent sample t test was performed between 2 groups.One-Way ANOVA analysis was performed among several groups.For multivariate analysis,multivariate linear regression analysis was performed.P ＜ 0.05 was regarded as statistically significant.Results The independent sample test revealed that the total cost of hospitalization for those with preoperative deep venous thrombosis (6.9 ± 1.0 × 105 (￥)) was significantly higher than for those without (5.0 ± 0.8 × 105 (￥)) and the total cost for those undergoing intramedullary fixation (5.5 ± 1.2 × 105 (￥)) was significantly higher than for those undergoing extramedullary fixation (4.4 ±0.7 × 105 (￥)) (P ＜ 0.05).The multiple linear regression analyses showed that preoperative deep venous thrombosis (t =11.750,P ＜ 0.001),perioperative transfusion (t =2.803,P =0.006),hospital stay (t =5.949,P ＜ 0.001) and intraoperative blood loss (t =2.885,P =0.005) were the independent factors influencing the total cost of hospitalization.Conclusions Preoperative deep venous thrombosis,perioperative transfusion,intraoperative blood loss,and hospital stay may influence the total cost of hospitalization for patients undergoing internal fixation of intertrochanteric fracture.More attention should be paid to control of the total cost of hospitalization in addition to ensuring the perioperative life safety.

Inflammatory bowel disease (IBD) is a chronic intestinal inflammatory disease with unknown etiology,including ulcerative colitis (UC) and Crohn’s disease (CD).At present,as the global incidence of inflammatory bowel disease is increasing year by year,the role of the intestinal micro ecology in disease evolution has attracted more and more attention,and as an important part of gut microbes system,the intestinal fungus'changes and effects in disease states are unclear.This paper reviews on the intestinal fungus in the occurrence,development of inflammatory bowel disease from the composition and distribution of the intestinal fungus and its relationship with the gut bacteria,and the immune reaction.

Objective:To analyze and compare the dose monitoring and control systems of two types of medical linear accelerator systematically from the view of mechanical and electrical engineering, and establish the method of the quality assurance of the dosimetry system and the solution in the routine maintenance.Methods: To systematically compare and analyze the system of dose monitoring and control for two types of linear accelerators (Precise Elekta and C-series of Varian Medical Systems) from twofold aspects that included machine physics and electric engineering of medical linear accelerators.Results:The monitoring system that took ionization chamber as center was established between connotation of machine physics and concrete realization of electromechanical engineering, and explained the difference and similarity between open type and sealed type of ionization chamber, and the theory that Precise series needn't be serviced by PFN.Conclusion: Based on the analysis of ionization chambers structure of medical linear accelerators on the principle of quality assurance system, proposed dosimetric testing and adjustment method, and provide reference for the establishment of quality assurance system and technical procedures of the maintenance engineering.