Kouzilan was selected as a potent hypotensive drug, and two fractions, K01 (hydrosoluble element) and K02 (fat-soluble element), were extracted from it. Thepresent study focused on its mechanism of vascular effects in vitro. The data showed thatK01 and K02 antagonized the contraction of isolated thoracic aortic ring of SD rat, indu-ced by norepinephrine (NE, 1 ?mol/L). The IC_(50) values of K01 and K02 were 1.98g/Land 1.15g/L respectively, and that of Verapamil (Ver) was 0.88 ?mol/L. K01, K02 andVer all shifted the dose-response curves of NE to the right in a noncompetitive antagonis-tic manner, and they also depressed the maximal efficacy of contraction. Added NE intoa calcium-free K-H solution to induce aortic vessel contraction (phase 1), then added cal-cium (2.5mmol/L) to induce continued contraction (phase 2), K01 depressed phase 2significantly. In lower concentration, K02 was similar to K01, however, in higher concen-tration, it depressed phase 1 and phase. 2 significantly, which was similar to Ver. Theseresults suggested that K01 and K02 had a vasodilative effect, which was related to theblockade of the receptor operated calcium channel (ROC). K02 inhibited both the calciumrelease from intracellar storage sites and the influx of extracellar calcium, but K01 onlyinhibited the latter.

With the advancement research on target therapy,more and more target drugs have been applied in the treatment of endometrial cancer,including anti-TKI or monoclone antibody and inhibitors in molecular signal pathways like mTOR,all of which have shown good therapeutic effects.As there were only on some phaseⅡclinical trials,so randomized stageⅢclinical trials are required to confirm these effects.In this review,the author discusses the advancement of target therapy in endometrial cancer.

The polymorphism of cytotoxic T lymphocyte associated antigen-4 (CTLA-4) was investigated by PCR-RFLP in 28 healthy volunteers and 64 patients with Graves′ disease (GD). The result suggests that the frequency of G49 allele of CTLA-4 exon 1 in GD patients issignificantlyhigherthanthat in normal controls (P

The Montreal Cognitive Assessment (MoCA) scale is a rating scale for rapid screening of mild cognitive impairment.In recent years,with the deepening of clinical research,the application scope of MoCA has been gradually expanded to the assessment of cognitive impairment-related diseases.This article reviews the clinical advances in research on MoCA in early screening of vascular cognitive impairment-no dementia

The changes of the levels of C-reactive protein,endothelin and nitric oxide before and after pioglitazone treatment in type 2 diabetic patients suggested that inflammation participated in the pathogenesis of diabetic macroangiopathy and pioglitazone can protect vessels

Hypoxic metabolism in solid tumor reduces chemoradiotherapy effect and become the obstacle in tumor therapy. Gene therapy is the most promising tumor treatment method, but currently using carriers cannot target tumors accurately. Anaerobic bacteria has become the transferring carrier in tumor gene therapy because it has tendency to hypoxia in solid tumors and can target solid tumors more accurately.

Bycomparingthe clinical internship ofFrench students in the Alsace Cancer Center of France(Centre Paul Strauss)with Chinese medical students,we found the differences and got some valuable implications.

Objective:To explore the changes of chemotherapy sensitivity of breast cancer MCF-7/ADM cells after Hiwi gene targeting silence, and to illuminate the role of Hiwi gene in resistant mechanism of breast cancer MCF-7/ADM cells.Methods:The MCF-7/ADM cells were transfected with Hiwi control,siRNA1 Hiwi gene and siRNA2 Hiwi gene as Hiwi control group, Hiwi siRNA1 group and Hiwi siRNA2 group.The expression levels of Hiwi gene mRNA and protein in breast cancer MCF-7/ADM cells were detected by Real-time PCR and Western blotting methods to judge the transfection rates in various groups.After transfection,the breast cancer MCF-7/ADM cells in various groups were treated with the different concentrations(0,0.1,0.5 and 1.0 mg·L-1) of adriamycin, and the cell resistant sensitivities were detected by MTT method.Results:Compared with control group(0 mg·L-1 adriamycin), the expression levels of Hiwi gene mRNA and protein inMCF-7/ADM cells in Hiwi siRNA1 and Hiwi siRNA2 groups were significantly decreased (P<0.01).Compared with control group, the survival rates of Cells in Hiwi siRNA1 and Hiwi siRNA2 groups were significantly decreased after treated with different concentrations of adriamycin,and the survival rates in Hiwi siRNA1 and Hiwi siRNA2 groups were significantly decreased(P<0.01);they were (48.15±6.28)% and (41.73±6.17)% when the concentration of adriamycin was 1 mg·L-1,and the sensitivities to the adriamycinwere obviously enhanced(P<0.01).Conclusion:The target silencing Hiwi genes in MCF-7/ADM cells is efficient.The sensitivity of MCF-7/ADM cells to adriamycin restores after Hiwi gene silencing.

After a descrption of the background of patron-driven acquisition ( PDA) or demand-driven acquision (DDA) and its short-term loan model, purchase model, auto-purchase model, librain-purchase model, and evi-dence-based acquisition model, the total amount of resources in common PDA platform, PDA models and their users were analyzed.The key problems in PDA implementation were pointed out, such as formulation of criteria for the development of resources, control of funds, mangement of MARC data, and assessment of PDA project, and sug-gestions were put forward for their solution .

Objective To evaluate the efficacy of 3+x mode for the reduction of unplanned extubation in the patients with stomach cancer. Methods 107 patients with the indwelling gastric tube of gastric cancer perioperative from June 2014 to September 2014 were collected as the observation group, and 101 patients with the indwelling gastric tube of gastric cancer perioperative from June 2013 to September 2013 were collected as the control group. Once preoperative education on stomach tube was performed in the control group, and 3+x education mode was used in the observation group, so as to compare unplanned extubation rate of two groups. Results The tube unscheduled decannulation rate in postoperative patients with gastric cancer in the control group was significantly higher than that in the observation group [4.95 %(5/101) vs 0 (0/107)], and there was significant difference (χ2= 5.168, P= 0.029). Conclusion 3+x mode education can significantly reduce unplanned extubation rate in operation patients with gastric cancer.