Objective:To explore a method of high successful rate to establish the rabbit VX2 carcinoma model, and monitor the tumor with ultrasound technique.Methods:Tumor block transplanting method was used to make the rabbit VX2 carcinoma model,and verify the tumor in rabbit′s liver with pathological section.Ultrasound technique was applied to detect liver echo, size and echo of tumor,generation and changing of blood vessels in tumor and to find out the characters of the rabbit VX2 carcinoma.Results:The rabbit VX2 carcinoma model was made successfully with tumor block transplanting method, and the success rate was 100%.The ultrasound technique could measure the size of tumor exactly, and it could monitor the changes of the echo and tumor blood vessels in carcinoma exactly at the right time.Conclusion:Tumor block transplanting method is a simple method to make rabbit VX2 carcinoma model with high successful rate. The ultrasound technique is an accurate、convenient and non-invasive method to monitor the carcinoma and blood vessels in tumor.The results provide experimental evidence in evaluating the efficacy of new techniques and therapeutics for liver carcinoma.

Mandibular teeth and sustentacular tissues extracted from a healthy adult were treated using CT scanning to obtain two-dimensional image, and then a three-dimensional image was established using computer reconstruction technique. Simultaneously, a three-dimensional finite element model of rigid fixed bridge was established in abutments with mandibular 56 deletion. A fixing load of 100 N was vertically and horizontally loaded on the middle site of bridge, and the abutments were grouped according to the proportion of alveolar bone absorption of 5% sub-interval to analyze the effects of 0 50% alveolar bone absorption on stress distribution of rigid fixed bridge. When the alveolar bone absorption was zero or less than 10%, the stress of rigid fixed bridge was evenly distributed on abutments with the vertical load of 4 7 MPa and horizontal load of 6 9 MPa; when the alveolar bone absorption was greater than 10%, the stress of rigid fixed bridge was concentrated on the abutments with the vertical load of 9-16 MPa and horizontal load of 14-23 MPa. This suggested hthat alveolar bone absorption had great effect on stress distribution of rigid fixed bridge in abutments with mandibular 56 deletion, namely a little absorption would cause the changes of stress distribution.

0.05)Conclusion There are some free cancer cells in the prepheral blood of some patients with gastric cancer before operation. Pulling and stimulating tumor in the operation may increase cancer cell dissemination.Simple ligation of peripheral veins around stomach could not completely prevent cancer cell dissemination in the peripheral blood.

Lung cancer is one of the malignant tumors with a high morbitity and mortality in the world.During its neoplastic development, a set of changes in protein expression occurs.The changed proteomic profile of cancers—tumor biomarkers could guide clinical decision-making.Proteomic methods are widely used in identification of tumor biomarkers.To reduce the mortality of lung cancer and improve its therapy,many studies on the identification of this tumor biomarker are being carried out.This review summarizes the main strategies and technology in proteomics and its application in identification of lung cancer biomarkers.

Objective: To investigate the relationship between circulating endothelial cell(CEC)(numbers),blood lipid levels and multiple organ dysfunction syndrome(MODS) and their clinical(significance).Methods: CECs were separated by isopyknic centrifugation method and blood lipid was(determined) in(35 MODS) patients and 20 healthy controls.CEC numbers and blood lipid were compared in both groups.(Acute) physiology and chronic health evaluation Ⅱ(APACHE Ⅱ) score was recorded to evaluate the severity of the MODS patients.The relationships were analyzed between APACHEⅡ score and CEC numbers,(between) APACHEⅡ and blood lipid levels and between CEC numbers and blood lipid levels.Results: CEC numbers in MODS patients were significantly higher than those in healthy controls(P

This article reviewed many different opinions on teeth-marked tongue, and made a discuss on the differentiation of symptoms and signs based on clinical practice. We consider that the teeth-marked tongue indicates the diseases that are caused by pathogenic dampness, while the correlation between such diseases and spleen deficiency still needs study.

Objective To research the clinical efficacy of alprostadil injection in treatment of patients with emergency percutaneous coronary intervention (PCI).Methods Eighty-five patients with acute myocardial infarction(AMI) were enrolled in this study,and they were randomly divided into treatment group (45 patients) and control group (40 patients).All patients were given 300 mg aspirin and 300 mg clopidogrel before operation,anti-platelet aggregation and long-term hpid regulation were given.On the basisof conventional treatment,0.9％ sodium chloride (NaCl) 100 ml and alprostadil injection 10 μ g were given in treatment group,2 times one day,a total of 14 d.After treatment for 1,6 months,the heart function and systohc function were assessed by echocardiography.High sensitive C reactive protein (hs-CRP),endothehn (ET)-1 and interleukin (IL)-6 were measured with ELISA,nitric oxide (NO) was measured with chromatometry.The basic clinical characteristic,inflammatory markers and major adverse cardiac events (MACE) were analyzed during in-hospital and follow-up period between two groups.Results The level of IL-6,hs-CRP,ET-1,NO in two groups had no significant difference before treatment and after treatment for 1 month (P ＞ 0.05).After treatment for 6 months,the level of IL-6,hs-CRP,ET-1,NO in treatment group had significant difference compared with that in control group [(1.04 ± 1.10) ng/L vs.(3.82 ± 1.34) ng/L,(1.5 ±1.2)mg/Lvs.(3.5 ±0.8) mg/L,(62.18 ± 20.22) ng/L vs.(85.56± 16.55) ng/L,(55.25 ± 8.25) μmol/L vs.(46.16 ±9.20) μ mol/L] (P ＜0.05).The level of left ventricular end-systolic volume (LVESV),left ventricular end-diastolic volume (LVEDV),left ventricular ejection fraction (LVEF) in two groups before treatment and after treatment for 1 month had no significant difference (P ＞ 0.05).After treatment for 6months,the level of LVESV,LVEDV in treatment group were significandy lower than those in control group [(82.5 ± 20.6) ml vs.(90.5 ± 20.1) ml,(130.5 ± 33.8) ml vs.(144.3 ± 28.6) ml],the level of LVEF was significantly higher than that in control group [(57.8 ±6.3)％ vs.(48.5 ±6.5)％],there was significant difference (P ＜ 0.05).The mortality rate and the incidence of MACE was 7.5％ (3/40),25.0％ (10/40)in control group,which were significantly higher than those in treatment group [4.4％ (2/45),13.3％ (6/45)],there were significant differences (P ＜0.05).The incidence of heart failure in control group was 22.5％(9/40),which was significantly higher than that in treatment group [11.1％(5/45)],there was significant difference (P ＜ 0.05).Conclusions Using alprostadil injection for patients with AMI after emergency PCI can prevent left ventricular remodeling,improve left ventricular function,and can reduce the incidence of MACE.Alprostadil injection can reduce the inflammatory reaction in patients with AMI,inhibit catecholamine release,inhibit platelet adhesion and aggregation,and stable plaques,reduce the incidence of MACE.

Objective Salient regions may express the main content (salient objects) in an image or scene.Since the number of implanted electrodes for visual prosthesis is limited,only the low-resolution image is used.Extraction of the salient regions may help capture salient objects in the scenes of visual prosthesis.Methods Salient regions detected by Itti model,one of salient detection models,have some differences with human visual perceptions and its salient objects boundary are also unclear.The Itti model was optimized by removing the orientation and the color characteristics,and converting the RGB image in an image or scene into the HSI color space with three new feature components of hue,saturation and intensity.In saliency map,the ratio of the salient point area in salient objects regions and the total salient point area was defined as salient accuracy and computed,which was used to compare the effects of the two methods of extracting salient map.Results Compared with the Itti method for extracting saliency map,the salient accuracy of the improved method was increased by about 20％,and the time for detecting the same salient regions by our method was shorter by nearly 50％.Conclusions The proposed algorithm can be an alternative method to extract salient map from a complex image or scene for retinal prosthesis with higher salient accuracy and less running time.

Anthranilic acid in the leaves of Isatidis indigotica Fort. was determined by first orderderivative UV spectrophotometry. The average recovery was 96.66％ and the coefficient of deviation was1.09％. The method showed no significant difference as compared with high performance capillary elec-trophoresis at 95％ confidence level. The method is simple, rapid and accurate.

After being restored, 69 cases of the two-implant-supported crowns were followed up for 5 years. Except 1 case failed after restored 5 years due to peri-implantitis, and there were no complications which can not be controlled in the other cases. The success rate of implant restoration in 5 years is 98.6%. The two-implant-supported single crown for molar is an effective method to restore the long gap of a single molar-loss.