Objective To investigate the clinical efficacy of acupoint application in treating senile hypertension of yin deficiency and yang excess type.Methods Sixty-six old patients with hypertension of yin deficiency and yang excess type were randomly allocated to treatment and control groups, 33 cases each. The control group took nifedipine controlled release tablets and the treatment group received acupoint application in addition. The quality of life score was recorded in the two groups before and after treatment. The clinical therapeutic effects were compared between the two groups.Results The total efficacy rate in reducing blood pressure was 90.0% in the treatment group and 84.8% in the control group; there was no statistically significant difference between the two groups (P>0.05). The total efficacy rate in improving the symptoms was 90.9% in the treatment group and 69.7% in the control group; there was a statistically significant difference between the two groups (P<0.05). There was a statistically significant pre-/post-treatment difference in the quality of life score in the two groups (P<0.05). There was a statistically significant post-treatment difference in the quality of life score between the treatment and control groups (P<0.05).Conclusions Acupoint application is an effective way to treat senile hypertension of yin deficiency and yang excess type. It can improve the quality of life in the patients.

Autonomic nervous system(ANS) activity plays an important role in the pathogenesis of cardiovascular disease,including organic cardiovascular disease(such as hypertension,coronary artery disease,etc) and functional cardiovascular disease (such as vasovagal syncope,postural orthostatic tachycardia syndrome,etc).Many methods was used to evaluate ANS function.Heart rate variability is a widely accepted method to assess cardiac autonomic nerve function.Autonomic nervous affected cardiac action potential and let electrocardiogram changes.Through the sympathetic and parasympathetic neurotransmitters which acting on the corresponding receptors.Electrocardiogram changes reflects autonomic nervous function.Present researches suggest that electrocardiogram has an important significance in the evaluation of autonomic nerve function in functional cardiovascular disease.

Objective To demonstrate the effect of bilateral adrenalectomy (ADX) on the susceptibility to NMDA-induced seizure and hippocampal CRH mRNA in young rats. Methods 60 Wistar rats at P10 were divided into control group, ADX group and Sham-ADX group. In the next day after operation, 7 mg/kg NMDA was injected to induce seizure, and incubation period (in minutes) as well as degree was evaluated. In situ hybridization was used to detect hippocampal CRH mRNA expression. Results Latency was (43.65 ± 2.96) minutes in control group, (35.05 ± 2.35)minutes in ADX group and (42.60 ± 1.90)minutes in Sham-ADX group. Latency in the ADX rats increased significantly (F = 73.73, P < 0.05). The seizure scale was (4.40 ± 0.60) in control group, (5.56 ± 0.76) in ADX group and (4.55 ± 0.76) in Sham-ADX group. The severity of seizures in ADX group increased significantly compared with the control and Sham-ADX groups (F = 15.52, P <0.05). CRH mRNA expression was 20% in control group, 55% in ADX group and 15% in Sham-ADX group. CRH mRNA in the ADX group was significantly elevated (χ2 = 9.048, P < 0.05). Conclusion Adrenalectomy exacerbates NMDA-induced spasm seizures in young rats , which might be related to CRH mRNA expression in the hippocampus.

Objective To explore the clinical effect of inverted L-shaped incision combined with medial canthal tendon plication for moderate and severe epicanthus correction.Methods Improved method of inverted L-shaped incision combined with medial canthal tendon plication were performed in 42 cases of moderate and severe epicanthus.The epicanthus covered more than 50％ lacrimal caruncle with single-fold eyelids or unconspicuous double-fold eyelids.Among them,40 cases received doubleeyelid plasty simultaneously.Results The follow-up time of 38 cases ranged from 6 to 24 months.37 cases received double-eyelid plasty simultaneously.All the patients obtained satisfactory results aesthetically,the contours of two eyelid fissures and the double-eyelid radian looked more natural postoperatively.There was no reoccurrence of epicanthus.Mild scar proliferation was observed in all of the patients in the early 1-2 months of postoperative period and then would fade within three months.Conclusions It is effective to correct epicanthus by improved method of inverted L-shaped incision combined with medial canthal tendon placation.

There were 17 literatures related to the clinical applications of natural killer cells in hematological diseases in the 18th annual meeting of the European Hematology Association (EHA).Here an overview of these literatures is given that helps to tnderstand how natural killer cells can be explored for future clinical interventions.

In recent years, the incidence of the allergic skin diseases is increasing as the growing of the pressure, the changes of the environment and other factors. The clinical features of the allergic skin diseases are easily repeated and in long course. It is found that the incidence of clinical allergic skin diseases, in addition to the season, diet and emotional relevant, is also closely linked to the physical deterioration of patients. Therefore, in addition to the emphasis on the treatment of the current symptoms of patients, adjustment and treatment of phsical constitution of patients and improvement of the adaptability of patients to environmental changes should attract attention, thereby reducing the frequency of incidence and alleviating the symptoms. The application of the abdominal acupuncture combined with body acupuncture for the improvement of the symptoms and physical constitution of allergic skin disease patients has obvious efficacy, which should be applied and promoted in clinic.

Objective To investigate the efficacy of dexmedetomidine in reduce of hyperalgesia in patien with remifentanil anesthesia.Methods 84 patients scheduled for laparoscopic hysterectomy from December 2014 to April 2016 were selected and randomly divided into two groups: the observation group and the control group,42 cases in each group,two groups were intravenous infusion of remifentanil,propofol and inhaled sevoflurane to maintain anesthesia.In the observation group,0.6μg/kg of dexmedetomidine was given 40minutes before the end of the operation(adding to 100mL 0.9％sodium chloride solution,infusion within 10minutes),the control group was given the same volume of 0.9％sodium chloride solution.The visual analogue scale(VAS)and the bruggrmann comfort scale(BCS)were used to evaluate the postoperative pain and comfort of the two groups of patients at different time points,and compare the incidence of adverse events between the two groups.Results The VAS scores of the two groups were significantly different at 1h(T1),4h(T2)and 24h(T3),and the VAS score of the observation group was significant better than that of the control group,the difference was statistically significant(P<0.05),the BCS scores of two group were compared at T1 and T2,the difference was statistically significant(P<0.05),but there was no significant difference between the two groups at T3.The incidence of adverse reactions in the observation group was lower than that in the control group,the difference was statistically significant(P<0.05).Conclusion Dexmedetomidine can relieve and prevent the incidence of hyperalgesia and adverse reactions in patients with remifentanil anesthesia,and improve the analgesic effect of postoperative patients.

OBJECTIVE:To probe into the situation of drug use that goes beyond the scope of package inserts in pediatrics of our hospital and to promote clinical rational drug use.METHODS:A total of 3 142 prescriptions between Mar.and Apr.2006 collected from the pediatric out-patient department of our hospital were analyzed in accordance with the contents of the related drug package inserts.RESULTS:Of the total 3 142 prescriptions,345(11.0%) involved drug use that goes beyond the scope of package inserts,leading the list were prescriptions of infants,accounting for 71.3% of the total.The problems such as age(52.2%) and indication(36.5%) were the main type;respiration system drugs(48.4%) and the antibiotics(40.6%) were the main drug kinds involved.CONCLUSIONS:It's widespread for drug use that goes beyond the package inserts in the pe-diatrics,which conforms to evidence-based medicine and the spirit of philosophy and ethics,but against the principle of law,which thus should be given fully attention in the clinic.

This article described the importance of mechanism refonil in the graduate student training and presented its contents.Also,It summarized the characteristics of the reforill proposals in some universities,and analyzed the problems required to attend and solve ia the refogln.The characteristics of the proposals included to set up innovative fund by university,to perform the student awarded police of tutor leading,to establish the scientific system in recruiting students.By analyzing the problems developed during the reform four measures were lannched,which included to establish evaluating system in assessing students,to create a good relationship between students and tutors,to improve the ability and morality of tutors,and to adjust the management method of research funding.It would be possible to reach the objective of perfecting the reform proposal during the implementation of the measures.

Objective:To develop a method for the determination of natural benzoic acid in Mesona chinensis Benth. with different production places and storage time. Methods:HPLC was adopted, the column was an Agilent ZORBAX SB-C18 (250 mm × 4. 6 mm, 5 μm) column, the mobile phase was methanol-0. 02 mol·L-1 ammonium acetate (5∶95) at the flow rate of 1. 0 ml·min-1 , the col-umn temperature was 35℃, the detection wavelength was 230 nm, and the injection vocume was 10μl. Results:Benzoic acid showed good linearity over the concentration range of 0. 1-10. 0 μg·ml-1(r=0. 999 9). The average recovery was 99. 59%(RSD=1. 57%, n=9). Natural benzoic acid in powered Mesona chinensis Benth. was different because of various production places. The maximum content of natural benzoic acid in Mesona chinensis Benth. was 141. 8 mg·kg-1, while the minimum content was 73. 8 mg·kg-1. The content of natural benzoic acid in Mesona chinensis Benth. stored for two years was declined obviously. Conclusion: The method with simple operation is accurate and reproducible. It can provide a basis for the further study of Mesona chinensis Benth. resources, and provide a reference for the content determination of natural benzoic acid in other plants.