1.An Appraisal of the Traditional Chinese Medicine Amber and Its Counterfeit
Chinese Traditional and Herbal Drugs 1994;0(01):-
Distinction between the traditional Chinese medicine amber and its counterfeit was studied by polarizing microscope and electron microscope, and trace clements analyse d by plasma spectroscopy. Results showed that the counterfcit of amber is opal, which contains the same thirty-five kinds of trace elements as coal amber. The total amount of Ca, Mg, Al,and Fe contained in opal is 10 to 70 times more than that contained in coal amber, whichcau provide a basis for the appraisal of coal amber and opal.
3.Several interpretation of improtant clinical trials on antihypertensive therapy
Chinese Journal of Practical Internal Medicine 2003;0(01):-
In the PROFESS and TRANSCEND trials,telmisartan(80 mg daily),compared with placebo,reduced systolic blood pressure by about 4 mmHg,but did not influence the primary composite cardiovascular endpoint.The difference between the ACCOMPLISH and ALLHAT trials in the amlodipine-diuretic comparison might be attributed to the combination of amlodipine with an angiotensin converting enzyme inhibitor in the ACCOMPLISH trial,which provided further evidence that by smoothly controlling blood pressure over 24 hours amlodipine prevent vascular events better than other antihypertensive drugs.
4.Transfection of adenovirus containing hepatocyte growth factor gene into adipose tissue-derived stromal cells
Keming WANG ; Jiguang MA ; Jie LUAN
International Journal of Surgery 2011;38(4):235-238,封3
Objective To observe the efficiency of infection of adenovirus containing hepatocyte growth factor(Ad-HGF) on adipose derived stem cells and to prove whether the valid HGF can appear after infection and the multiplicity of infection. Methods We use the digestion separation method and the attachingwall characteristic of the adipose-derived stem cells to separate the human adipose-derived stem cells. Adipose-derived stem cells were infected by the vector of adenovirus (Ad-GFP) which carries the GFP gene,and the GFP acts as the indicating gene to determine the infection efficiency of recombinant adenovirus to adipose- derived stem cells. HGF-ELISA was used to detect HGF as expression-secretion. Results The adherent cells displayed themselves as fibroblast in morphology. The primary cultured cells fusion can arrive to 70% - 80% in 7 - 10 days. The infected HGF can be highly expressed in 48hours. Conclusion Adenovirus can meditate the expression of HGF gene in adipose-derived stem cells effectively.
5.Therapeutic role of glucocorticoid in treating CMV severe pneumonia after kidney transplantation
Jiguang FEI ; Lizhong CHEN ; Changxi WANG
Chinese Journal of Organ Transplantation 2005;0(12):-
Objective To investigate the therapeutic role of glucocorticoid in treating cytomegalovirus (CMV) severe pneumonia after kidney transplantation. Methods Two groups of patients with CMV severe pneumonia after kidney transplantation were analyzed. The therapeutics for 12 patients of group A included the elimination of immunosuppressive agents such as cyclosporine (or tacrolimus) and cellcept, the use of antiviral drug such as gancyclovir, measures to prevent and cure other bacterial and fungal infections, supportive therapies and suck of oxygen or mechanical ventilation by respirators. Except for the above therapies, methylprednisolone was routinely injected to those 14 patients of group B. At the beginning, the dose of methylprednisolone was 120 mg/day to 150 mg/day. Three to five days later, the dose was decreased to 80 mg/day. The dose was further decreased to 40 mg/day when patients’ signs were improved. After patients’ signs were excluded, prednisone was taken orally in place of methylprednisolone. In our patients, methylprednisolone was used for average 12 days, ranging from 8 to 21 days. Results Among the patients of group A, 9 (75 %) were treated with mechanical ventilation by respirators, 7 ( 58.33 %) died and 2 ( 16.67 %) received dialysis due to dysfunction of the transplanted kidneys. Among the patients of group B, 4 ( 28.57 %) were treated with mechanical ventilation by respirators, 2 ( 14.29 %) died and no case with the transplanted kidney loss was found. There were significant differences between the two groups on the probability of using mechanical ventilation by respirators and the mortality (P= 0.047 and P= 0.038 respectively). In the patients of group B, no severe side effects caused by methylprednisolone were found. Conclusion The treatment with proper dose of methylprednisolone may extenuate effectively the inflammatory reaction from the CMV severe pneumonia after kidney transplantation while reduce the rejection related to the absence of other immunosuppressants and decrease the mortality and the rate of transplanted kidney loss.
6.A comparison of long-term effect and safety between tacrolimus and cyclosporine in cadaveric renal transplantation
Lizhong CHEN ; Changxi WANG ; Jiguang FEI
Chinese Journal of Organ Transplantation 2003;0(05):-
Objective To compare the long-term effect and safety between tacrolimus (FK506) and cyclosporine (CsA) in patients receiving cadaveric renal transplantation. Method A total of 210 patients were randomized to FK506 and CsA after cadaveric renal transplantation, and were followed up for 12-32 months for the variation of trough concentration in whole blood, the incidence of acute rejection and chronic rejection, one-year survival rate of patient/graft, variation of creatinine level, impairment of liver function and glucose metabolism and lipid metabolism, incidence of infection, and side effects. Results The variation of trough concentration of FK506 was similar to CsA. The incidence of acute rejection was significantly lower in FK506 group than in CsA group ( 16.3 % vs 33.0 % , P 0.05 ). The incidence of impaired liver function and impaired lipid metabolism and gingivitis and creatinine level three months after transplantation were significantly lower in FK506 group than in CsA group ( P
7.Fat grafting for correction of partial jaw deformity
Maosheng TIAN ; Zhicheng SUN ; Shurong LU ; Dong WANG ; Jiguang WANG
Chinese Journal of Medical Aesthetics and Cosmetology 2017;23(1):7-9
Objective To study the effects of fat grafting on partial jaw deformity.Methods A total of 19 cases of partial jaw deformity.were used fat grafting in past 2.5 years,from January 2012 to June 2015.All analyses were to find suitable fat grafting method for partial jaw deformiw.Fat graft was obtained by low pressure suction and low speed centrifugation.Results A lot of 15 patients in this group had been followed up for 6 months to 2 years.9 patients was satisfied with once fat grafting and 6 with twice.Conclusions Fat grafting provides a useful alternative to partial jaw deformity with normal occlusion.But it also remains to be seen what the forward effects of fat grafting on partial jaw deformity are.
8.Detection of immune cell subsets in renal allograft recipients before operation and its significance
Dongwei LI ; Longshan LIU ; Jiguang FEI ; Changxi WANG
Chinese Journal of Tissue Engineering Research 2013;(44):7675-7680
BACKGROUND:The immune cells of renal al ograft recipients have always been the hot spot of research. However, there are few studies addressing the immune cellsubsets in renal al ograft recipients before operation. OBJECTIVE:To investigate the proportional distribution of immune cellsubsets in renal al ograft recipients before operation. METHODS:Fifteen de novo living-related renal transplant recipients were enrol ed in this study with 15 healthy volunteers, aged 18-40 years, as healthy controls. Flow cytometry was employed to observe the proportion of the immune cellsubsets by extracting peripheral venous blood of al participants. RESULTS AND CONCLUSION:In the renal al ograft recipients, the proportions of CD4+CD25+T cells, the proportion of CD4+CD25+/CD4+T cells, CD19+B cells, CD19+CD5+B cells, CD19+CD27+B cells, NKG2A/NK cells, and NKG2A/NKG2 cells were al lower than those in the healthy controls;however, the proportion of CD38+IgD-/CD19+B cells and NKG2D cells were higher than those in the healthy controls. The difference of the proportion of immune cellsubsets aforementioned between the two groups was statistical y significant (P<0.05), while no difference was observed in other subsets. Immune cellsubsets in renal al ograft recipients before operation could be used to assess the immune status of the recipients, and also could be seen as the basal control for postoperative immunological monitoring.
9.AN ANATOMICAL CLASSIFICATION OF KIDNEY IN MAMMALS
Yi ZHANG ; Ping DONG ; Qijun WU ; Zhanyun WANG ; Jiguang YANG
Acta Anatomica Sinica 1957;0(04):-
Based on the anatomical study of kidney in mammals of 27 families and 163 species, the authors discovered Anoa depressicornis type kidney, and obtained the evidence of morphological transition between Ox-type kidney and Pig-type kidney. After observing the renal papillary structure of Budorcas taxicolor, the evidence of fact and theoretical bases of the biological view was proved that the general papillae are developed from the multi-papillae kidney. The author advanced a new type of leaf-shaped multipapillae kidney. The authors suggest to classify the mammalian kidneys principally into 2 groups, i. e, simple and composite kidney, and subdivide them into 4 types, i. e, multipapillae and concentrated papillae for simple kidney and leaf-shaped multipapillae and ball-shaped mono-papillae for composite kidney. In contrast to the previous classification, the 2-group 4-type classification recommended here with its solid anatomical foundation is conformable with the viewpoint of biology, and is more practical and integrated.
10.Analysis of protective earth requirement for touched metal parts of medical electrical equipment
Jiguang LIU ; Wei WANG ; Zhenjin DENG ; Qin XU ; Yong YIN
Chinese Medical Equipment Journal 2017;38(2):95-97,108
Objective To study the protective earth requirement for touched metal parts of medical electrical equipment to improve the manufacturer's understanding on national standards.Methods The isolation methods for the touched metal parts in national standards were studied,and the principle of protective earth combined with insulation separating was analyzed.The characteristics of the second isolation method was discussed,and the respiratory system structure and electroshock protection of the anaesthetic machine were taken as examples.Results The touched metal parts with no measures for grounding,double isolation or reinforced insulation was determined by structure detection to meet the requirements of national standards in case basic insulation ceased to be effective and the parts were uncharged.Conclusion The necessities to girt the touched metal parts with measures for grounding,double isolation or reinforced insulation should depend on the understanding on national standards and electrical construction of the equipment under test by structure detection.