Objective To develop and validate the real-time fluorescent quantitative PCR(Q-PCR)method for the detection of 8 murine viruses. Methods The specificity,sensitivity and precision of the Q-PCR method were verified by four laboratories,and the virus simulated contamination test and blind sample detection were carried out simultaneously,of which the detection results were compared. The Q-PCR method was used to detect 26 batches of monoclonal antibody cell lines for SARS-CoV-2 vaccine production and 15 batches of other products of murine origin. Results The Q-PCR method used for detecting 8 kinds of murine viruses had no cross reaction with the same family and genus or other murine viruses. Except the sensitivity of laboratory 2 to ectromelia virus(EctV/Mouse Pox,MPV)was 2 × 10~2copies/μL,the sensitivity of laboratory 2 to other 7 viruses and 3 other laboratories to 8 murine viruses was 2 × 10~1copies/μL. Except the inter-assay CV of the copy number of mouse adenovirus(MAdV)detected by laboratory 3 was 37. 58%,the intra-assay and inter-assay CVs of the Ct and copy number of other 7 viruses detected by laboratory 3 and those of 8 viruses detected by other 3 laboratories were all less than 25%.The sensitivity of virus simulated contamination test met the parameter requirements. The coincidence rate of blind sample detection results by 4 laboratories was 100%. All the 26 batches of monoclonal antibody cell lines for SARS-CoV-2 vaccine production and 15 batches of other murine derived products were negative for 8 murine viruses. Conclusion Q-PCR method for murine virus has good specificity,sensitivity and precision,and can be used for the detection of murine derived biological products.

AIM:To establish HPLC method for making a assay of nucleosines in Ningxinbao Capsules(Cepha-(lospovium) sinensis Chen.sp.nov). METHODS:The assay was performed on a C_(18) column(4.6 mm?250 mm,5 ?m).The mobile phase was composed of phosphate buffer(pH 7.8-8.0)-acetonitrile(83∶17).The flow rate was at 1.0 mL/min.The detection wavelength was at 260 nm. RESULTS:The linear ranges of uridine,adenosine and inosine lay in the concentration range of 4.168-83.36,4.480-89.60,5.230-104.6 ?g/mL,respectively(r=0.999 9). The average recoveries were 0.4%,1.8%,2.5%,respectively. CONCLUSION:The method is simple,rapid.It is suitable for the assay of nucleosines in Ningxinbao Capsules.

Objective The effects of nemo-like kinase(NLK)in inflammation of central nervous system were explored. Methods An animal model with central nervous system inflammation disease induced by intracerebroventricular infusion of lipopolysaccharide was constructed. A model of neuronal apoptosis induced by glutamate in PC12 cells was constructed. A pcdna 3.1-NLK over-expression plasmid and a pSilencer 4. 1-CMV-NLK small interfering plasmid were also constructed. The expression and location of NLK were detected using western blot analysis, double immunofluorescent staining, and cell counting kit-8 assay. Results The protein level of NLK was reduced after induction of inflammation in the brain(t =2. 718-3. 106, all P ＜0. 01), and NLK was only expressed in both cortical and hippocampal neurons. At the cellular level, NLK expression was gradually reduced along with neuronal apoptosis induced by glutamate in PC12(t =4. 032, P ＜0. 01). Over-expression of NLK would reduce the apoptosis of neurons induced by glutamate(t =3. 930, P ＜ 0. 01). Conversely, interfering the expression of NLK would enhance neuronal apoptosis(t = 2. 845, P ＜ 0. 01). Conclusion NLK can protect neurons by inhibiting apoptosis in the process of central nervous system inflammation.

5-fluorouracil (5-Fu) as an antimetabolite drug,has been widely used in the treatment of various solid tumors.However, due to the heterogeneity of tumor and the impact of the individual, some patients still have drug resistance or serious lethal toxicity reaction.The text focuses on the research progress of pharmacogenomics and pharmacogenetics of some forecast elements which affect the efficacy and toxicity of 5-Fu,and discussed their guiding role on the choice of chemotherapy drugs.

The determination of fraxetin A and fraxetin B in Cortex Fraxini have been carried out by HPLC on the stationary phase:octodecyl-silicohydride bonded silica gel column. The mobile phase was methanol-water(24:76). The detection wavelength was at 348nm. There was a good linear relationship in a concentration range of 16?g/ml~176?g/ml. The correlation coefficients were 0. 9995 and 0. 9994,respectively. The recovery of the added sample were 99. 1 ?1. 5% (n=5) for fraxetin A and 99.8?2. 5%(n=5) for fraxetin B. This method showed 0. 8% of within-day accuracy and 6. 2% of between-day accuracy,involving the advantages of simplicity,quickness and accuracy.

Objective: To establish the identification of protein compositions in Rhizoma Arisaematis through researches of its taxonomy, pharmacognostic anatomy and chemical compositions.Methods: The protein compositions in Rhizoma Arisaematis were identified by HPCE taking potassium phosphate boric acid buffer solution (pH=9.0) as an electrophoretic buffer. The electric sampling time was 10s, 10kV; Separating pressure 25kV; column temperature=28℃;?=205nm; integral determination sensitivety value=0.05; sensitivity=0.1 AUFS. Also, the raw plant identification, medical material characteristics and microscope identification were performed. Results: Combined with pharmacognostic and microscopic identification, experiments indicated that herbs of Arisaematis genus have similar HPCE chromatographic profile but different finger peaks. Condlusion: This study provides a better basis for the identification of Rhizoma Arisaematis.

Activation of HGF/Met has been complicated in tumorigenesis, invasion and metastasis of various tumors. We summarize current knowledge on their association with invasion of thyroid carcinoma. By discussing the expression of Met and its possible mechanism and analyzing the activation of Met with tumor motility, angiogenesis and inflammatory reaction, we conclude that the research of the possible role of HGF/Met in thyroid carcinoma will advance knowledge on the diagnosis and management of this disease,as well as tumor invasion.

At present, the roles of neural plasticity and motor functional recovery in stroke have increasingly been paid attention to. The remodeling of cerebral cortex after stroke can complement the function of injured tissues, which has been confirmed by animal experiments. Functional neuroimaging technique has been gradually used in the evaluation of neural plasticity.

Adenoid cystic carcinoma is regarded as one of the most common malignant tumors in parotid gland. The local control rate and long term survival rate of salivary gland adenoid cystic carcinoma, which tends to spread along the perinerve and metastasis distantly, are not good. This review focused on the advances in the treatment of adenoid cystic carcinoma of parotid gland.

Objective To explore feasibility and clinic results of the MIPPO (minimally invasive percutaneous plate osteosynthesis) technique in treatment of humeral fractures. Methods 14 patients with humeral fracture underwent the MIPPO operation in our department. 7 of them were injured in a traffic accident, 4 in sports and 3 in daily life. There were 11 males and 3 females, with their ages ranging from 16 to 72 years. Of the 14 cases, 9 were proximal ones and 5 mid- distal ones, 3 were multiple injuries and 1 pathological fracture. Results The average intra- operative time was 80 minutes (ranging from 55 to 130 minutes). No patient received blood transfusion. The intra- operative blood loss was 100 to 200 mL. 14 cases were followed up for 8 weeks to 14 months. Their cuts healed at the primary stage. Their shoulder and elbow functions were fine. The fracture segments got satisfactory reduction with good apposition and alignment radiologically. The callus appeared 4 to 8 weeks postoperatively. Conclusion MIPPO is a safe and effective treatment for the humeral fracture with the benefits of less invasion, fewer complications and higher union rate.