Objective To discuss the expression and its significance of neuron specific enolase(NSE) and soluble-protein 100(S100) in Hirschsprung′s disease(HD) and its allied disease.Methods Clinicopathologic data were reviewed from 120 cases of suspected HD patients.According to the morphological characteristic of the nerve stanza cell stained with HE under the microscope.NSE and S100 immunohistochemical staining was performed in 29 cases.Then we compared the effect of the staining with analyzed the distribution.Results The number of the nerve plexus and ganglion cell of allied HD was significantly higher than that in normal control group(P

Objective To explore the control measures of drug induced liver disease.Methods The age,disease drugs,clinical manifestations,treatment and prognosis of 162 cases with drug induced liver disease were analyzed retrospectively.Results Drug induced liver disease incidence in different age groups are slightly more old age group,Lead to liver damage to many types of drugs,a common anti-TB drugs and other lipid-lowering medicine and Chinese herbal medicine and health drugs can not be ignored.Clinical performance of drug-induced liver disease is different,mostly had good prognosis after treatment,a few turn into cirrhosis,a very small number of fulminant hepatic failure or even death.Conclusion Avoid the use of the drug that may injure the liver,liver protection must also drugs should be regularly reviewed liver function.

OBJECTIVE: To explore the clinical application value of using needle electrode in transurethral plasmakinetic resection of bladder tumor around ureteral orifice. METHODS: Retrospective analysis was performed on the clinical data of 16 cases who had bladder tumors around ureteral orifice and underwent transurethral resection using plasmakinetic needle electrode in Department of Urology, Peking University International Hospital from June 2015 to December 2019. There were nine cases with the tumor of one to two centimeters from the ureteral orifice. The rest of the seven cases had tumor that was within one centimeter from the ureteral orifice, including two cases whose ureteral orifice was invaded by the tumor. All the patients studied were diagnosed before surgery and contraindications were excluded. The plasmakinetic needle electrode was used to treat the tumor with en bloc resection, and all the excised tissue was sent for pathological examination. Intravesical chemotherapy and postoperative follow-ups were performed. Statistical analysis was performed on the operation time, the incidence of obturator nerve reflex, the peri-operative bleeding, the parameters of indwelling ureteral catheter or double-J stent, the incidence of postoperative hydronephrosis, the clinical stage of tumor, and the recurrence rate. RESULTS: The operation was successfully completed for all the sixteen cases. The operation time was 16 to 57 minutes, with an average of (32.6±11.8) minutes. No obvious obturator nerve reflex and perioperative bleeding occurred in all the patients. Ureteral catheters were indwelled prior to the operation of tumor resection in seven cases. Four of the seven cases had the ureteral catheters remained while the rest three were replaced by double-J stent after surgery. Postoperative pathological analysis showed that all the tumors were urothelial carcinoma, including 9 cases of low grade and 7 cases of high grade. Pathological staging: 10 cases were in Ta stage, 5 cases in T1 stage, and 1 case in T2a stage. All tumor bases and lateral margins were negative. All the patients received 3-56 months, with an average of (26.0±18.1) months of follow-up. There was no case of upper urinary tract hydronephrosis or tumor recurrence. CONCLUSION The transurethral plasmakinetic resection of bladder tumor using needle electrode can realize en bloc tumor resection without obturator nerve reflex and reduce the risk of ureteral orifice injury. It is a safe and effective surgical method for treating bladder tumors around the ureteral orifice.
Carcinoma, Transitional Cell ; Electrodes ; Humans ; Neoplasm Recurrence, Local ; Retrospective Studies ; Urinary Bladder Neoplasms/surgery*

Adult ; Histiocytosis, Sinus ; Humans ; Male ; Nasal Cavity ; Paranasal Sinuses

Objective To study the effects of continuous oxygenated blood microperfusion on rat hearts preservation.Methods Forty-five Wistar rats were randomly divided into 3 groups.The isolated rat hearts were continuously microperfused with oxygenated blood at 4℃in experimental group,those in control group microperfused with St.Thomas solution and those in solution group were preserved with St.Thomas solution.After preservation the hemodynamic indexes of rat hearts were measured using Langendorff system,including the left ventricular end-diastolic pressure (LVEDP),the left ventricular systolic pressure (LVSP),the left ventricular developed pressure (LVDP) and the rate of the left ventricular pressure change (?dp/dt),then the contents of adenosine triphosphate (ATP) were determined by high-performance liquid chromatography and myoeardium ul- trastructures were observed under an electric microscope.Results After 30 min perfusion of Langen- dorff system,the LVSP,LVDP and?dp/dt were (38,25?3.84) mm Hg,(32.54?4.01) mm Hg and (1080?123) mm Hg/s respectively in experimental group;(34.48?4.68) mm Hg,(19.27?4.63) mm Hg and (935?196) mm Hg/s respectively in control group;(32.14?4.95) mm Hg, (16.99?4.85) mm Hg and (825?302) mm Hg/s respectively in solution group.The hemodynamic indexes in experimental group were superior to those in control group and solution group (P

Objective To investigate the effect of diazepam on blood-brain barrier ( BBB ) after radiation-induced brain injury. Methods Two hundred and forty healthy male SD rats weighing 240-270 g were randomly divided into 4 group with 60 animals in each group: Ⅰ D-R group received intraperitoneal diazepam 5 mg?kg-1 30 min before irradiation; Ⅱ N-R group received no diazepam before irradiation; Ⅲ D-SR group received intraperitoneal diazepam 5 mg? kg-1 30 min before sham irradiation and Ⅳ N-SR group received no diazepam before sham irradiation. The animals were placed in a prone position awake without any anesthetics. The whole brain was irradiated by 4 MeV electron beam at a single dose of 30 Gy. The changes in BBB were assessed by (1) magnetic resonance imaging (MRI) with the T1-weighted images, T2-weighted images and MRI enhancement images induced by Gd-DTPA; (2) Evans blue (EB) assay; 2% EB 3 ml?kg-1 was injected via femoral vein and 3 h later the animals were decapitated and EB content in the brain tissue was determined by spectrophotometry; (3) The expression of vascular endothelial growth factor (VEGF) in the brain tissue determined by immuno-histochemistry before irradiation ( T0 ) ( baseline) at 6 h (T1 ), 1 day ( T2 ) , 1 week ( T3 ) and 1 month ( T4 ) after irradiation.Results In group Ⅱ (no diazepam before irradiation) the signal intensity of T1-weighted images significantly decreased while the signal intensity of T2-weighted images and the enhancement rate significantly increased at T4. However in group Ⅰ (diazepam was given before irradiation) the changes in the MRI were significantly attenuated. The EB content at T1, T3 , T4 and the expression of VEGF at T1 , T, were significantly higher in group Ⅱ than in group Ⅰ . Conclusion Diazepam given before irradiation can protect BBB against irradiation injury by decreasing the expression of VEGF in the brain tissue and BBB permeability.

long -term effect.All therapeutic modalities, including acu - moxibus-tion, Chinese materiamedica, and both acu - moxi-bustion and Chinese medicament, are all superiorte western drugs. The last modality is especially satisfactory due to its safety, free from side effect and stable

Objective To analyze the correlation between serum interleukin-18(IL-18) level and changes of heart function in children with viral myocarditis(VM).Methods Seventy-two cases with VM were selected from Tianjin Children's Hospital.Control group were selected from the patients in surgery outpatient department.The patient group was divided into 3 groups which were named non-heart failure group,heart failure group and cardiogenic shock group.The Pillip Sonos 5500 type echocardiogram was applied to measured the left ventricular fractional shortening(LVFS),ejection fraction (EF),left atrium inner diameter(LAD),left ventricular end diastolic inner diameter (LVEDD)and left ventricular end systolic inner diameter(LVESD) in patient group,descripted function change of their heart.ELISA method was used to measure the content of IL-18 in 15 cases of control group and 36 cases of patient group.All data were analyzed by SPSS 11.5 software.Results 1.The LVESD in shock group were larger than that in non-heart failure group and heart failure group,abnormality probability of LVFS and EF step up from non-heart failure group to heart failure group and to shock group.2.The serum IL-18 level in patient group was higher than that in control group (P

Objective To study the relationship between the renal hemodynamic changes and the course of the liver diseases and demonstrate weather the renal blood flow resistance index(RI)could be a useful index used to predict the hepatorenal syndrome(HRS)in the decompensated cirrhosis.Methods Select 41 patients diagnosed as decompensated cirrhosis from the patients examined by color Doppler ultrasonography at the Infectious Disease Department of Peking University First Hospital from April 2004 to July 2006.Test the serum creatinine,serum total bilirubin and INR,confirm the cause of the cirrhosis,and then calculate the MELD score.Detect by color Doppler ultrasonography to get RI.Results No correlation was found between RI and the patients's age,hemoglobin and systolic pressure.RI was found to be directly related with MELD score(r= 0.502,P

Objective To observe the Leu72Met single nucleotide polymorphism (SNP)of ghrelin gene and the relationship with essential hypertension (EH).Methods Polymerase chain reaction -restriction fragment length polymorphism (PCR -RFLP)was used to detect the Leu72Met SNP of ghrelin gene in 210 EH patients and 220 healthy controls.The plasma ghrelin was detected by radioimmunoassay method collected from all subjects. Results There were three types of polymorphism of ghrelin gene at the base site Leu72Met.There were significant differences in the genotypes (CC,CA,AA)and alleles (C,A)between the EH patients and the controls (χ2 =6.054,P =0.048;χ2 =5.866,P =0.015).In EH group,the plasma ghrelin level in subjects who were homozygous CC without mutant was not only significantly lower than those who were heterozygous CA,but also lower than those who were nucleotide homozygous mutant AA (t =-8.738,P =0.000;t =-5.103,P =0.000).The patients with CC genotype had higher SBP (t =4.298,P =0.000;t =2.236,P =0.019)and lower HDL -C (t =-11.682,P =0.000;t =-7.872,P =0.000).The patients with A allele had lower plasma ghrelin (t =-16.264,P =0.000), HDL -C (t =-15.332,P =0.000)and higher SBP(t =3.800,P =0.000),DBP(t =11.895,P =0.000),and LDL -C (t =38.401,P =0.000).Conclusion The Leu72Met SNP of ghrelin gene is significantly related to the susceptibility of EH.Base mutation C to A reduced the incidence of EH.The Leu72Met polymorphism of ghrelin gene is related to the plasma ghrelin,blood pressure and blood lipid metabolism.Base mutation C to A elevated plasma ghrelin,and lowered blood pressure and blood lipid.