1.Molecular Characterization of a New Hantaan Virus Howang Strain.
Journal of the Korean Society of Virology 1997;27(1):59-68
Hantaan virus Howang strain which isolated from the blood of severe case of Korean hemorrhagic fever is more virulent than HTN 76/118 and showed different RFLP from partial PCR amplified M genome segment to established Hantaan serotype viruses. We have determined the nucleotide sequence of the M and S genome segments and compared to HTN 76/118. The M and S segment of Howang strain has 3615 and 1696 nucleotides long, respectively. The M segment sequence of Howang strain is one mucleotide shorter than HTN 76/118. The sequence data of Howang strain shows 93.5% homology to HTN 76/118. One long open reading frame, which stoats from 41nt. to 3448nt. of the M segment and from 37nt. to 1326nt. of the S segment, exist to on complementary sense of the virus genome. There are no significant difference between HTN 76/118 and Howang strain on hydrophobicity of deduced polypeptides, but has slight difference on secondary structure.
Base Sequence
;
Genome
;
Hantaan virus*
;
Hemorrhagic Fever with Renal Syndrome
;
Hydrophobic and Hydrophilic Interactions
;
Nucleotides
;
Open Reading Frames
;
Peptides
;
Polymerase Chain Reaction
;
Polymorphism, Restriction Fragment Length
2.Discrimination of Hantaviruses from the Tissues of Infected Hamsters to 5 Different Serotype Hantaviruses by Nested RT-PCR using Hantavirus Serotype Specific Primers.
Journal of the Korean Society of Virology 1997;27(1):49-57
We developed a sensitive, nested reverse transcription-polymerase chain reaction (RT-PCR) to detect Hantaan, Seoul, Belgrade, Puumala and Sin Nombre viruses in animal tissues. Total RNA was extracted from blood, lung or kidney samples of experimentally-infected hamsters by using the guanidine isothiocyanate buffer-acid phenol-chloroform method. Genus-reactive outer primers were derived from the consensus region of the G1 gene sequences of several hantaviruses. Serotype-specific primers were selected within the region amplified by the outer primers. To examine the sensitivity and specificity of the test, we diluted known quantities of Hantaan, Seoul, Belgrade, Puumala and Sin Nombre viruses in human or hamster immune sera before performing the nested RT-PCR. We could detect as little as 1 pfu of virus, even in the presence of high-titer neutralizing antibodies, and the serotype-specific primers amplified only homologous serotype viruses. RT-PCR with these primers demonstrated virus in the blood of experimentally-infected hamsters as early as four days to as late as 30 days after infection.4 comparison of a standard immunofluorescent antibody screening test (IFAT) to nested RT-PCR with RNA extracted from lung or kidney tissues of the hamsters, demonstrated that RT-PCR to be more sensitive for identifying viruses in these tissues.
Animals
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Antibodies, Neutralizing
;
Consensus
;
Cricetinae*
;
Discrimination (Psychology)*
;
Guanidine
;
Hantavirus*
;
Humans
;
Immune Sera
;
Kidney
;
Lung
;
Mass Screening
;
RNA
;
Sensitivity and Specificity
;
Seoul
;
Sin Nombre virus
4.Seroepidemiologic study of Hantavirus infection of wild birds and bats in Korea.
Ho Wang LEE ; Luck Ju BAEK ; Yun Tai LEE
Journal of the Korean Society of Virology 1991;21(2):127-134
No abstract available.
Birds*
;
Chiroptera*
;
Hantavirus Infections*
;
Hantavirus*
;
Korea*
;
Seroepidemiologic Studies*
5.Immunohistochemical study on the changes in the distribution of T & B lymphocytes by administration of mitogens and T cell dependent antigen in the mouse spleen.
Wang Jae LEE ; Ka Young CHANG ; Kwang Ho LEE
Korean Journal of Anatomy 1991;24(4):422-437
No abstract available.
Animals
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B-Lymphocytes*
;
Mice*
;
Mitogens*
;
Spleen*
6.A transmission electromicroscopic study of the synoviocytes in the knee joint of the mouse after birth.
Douk Ho HWANG ; Kyu Jung CHO ; Wang Jae LEE ; Kwang Ho LEE ; Ka Young CHANG
Korean Journal of Anatomy 1992;25(1):82-90
No abstract available.
Animals
;
Knee Joint*
;
Knee*
;
Mice*
;
Parturition*
8.Isolation of the Hantaviruses from the Lungs of Bandicota indica Captured in Indonesia and Thailand.
Young Dae WOO ; Yong Kyu CHU ; Ho Wang LEE
Journal of the Korean Society of Virology 1998;28(2):157-164
Various hantaviruses were isolated from HFRS patients and various rodent species, in many parts of the world. Bandicotas were captured at Yogyakarta, east region of Sumatura island, Indonesia; and 4 rodents species including Bandicotas were captured at Chiang Rai in Thailand during 1995. Sera were collected from captured andicotas and other rodent spicies were screened for antibody test against Hantaan (HTN), Seoul (SEO), Puumala (PUU) and Sin Hombre (SN) viruses by immunofluoresence antibody assay (IFA). Hantavirus antigen in lung tissues were tested by IFA. Among 55 captured Bandicota indica in Indonesia, 14 (25.5%) were antibody positive against HTN, SEO, PUU and SN virus. Hantavirus antigen were detected from 5 (9.0%) out of 55 lungs tested. Among 34 captured Bandicota indica in Thailand, 9 (26.5%) were antibody positive against HTN, SEO, PUU and SN virus. Among 34 lungs tissues of Bandicota indica examined, 3 (8.8%) were antigen positive. In other rodent species, antibody positive against Hantaviruses of Rattus rattus, Rattus losea and Mus cervicolor were 4/62(6.5%), 5/25(20%), 1/1(100%), respectively. But no one has antigen in their lung tissues. Antigen positive lungs suspension were inoculated into vero E6 cells for virus isolation and 4 viruses were isolated from Indonesian Badicota and 3 viruses from Thailand.
Animals
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Hantavirus*
;
Hemorrhagic Fever with Renal Syndrome
;
Humans
;
Indonesia*
;
Lung*
;
Mice
;
Murinae*
;
Rats
;
Rodentia
;
Seoul
;
Thailand*
9.Comparative study od serologic diagnostic tests against Hantaan virus.
Ki Joon SONG ; Luck Ju BAEK ; Ho Wang LEE
Journal of the Korean Society of Virology 1991;21(2):87-103
No abstract available.
Diagnostic Tests, Routine*
;
Hantaan virus*