Hantaan virus Howang strain which isolated from the blood of severe case of Korean hemorrhagic fever is more virulent than HTN 76/118 and showed different RFLP from partial PCR amplified M genome segment to established Hantaan serotype viruses. We have determined the nucleotide sequence of the M and S genome segments and compared to HTN 76/118. The M and S segment of Howang strain has 3615 and 1696 nucleotides long, respectively. The M segment sequence of Howang strain is one mucleotide shorter than HTN 76/118. The sequence data of Howang strain shows 93.5% homology to HTN 76/118. One long open reading frame, which stoats from 41nt. to 3448nt. of the M segment and from 37nt. to 1326nt. of the S segment, exist to on complementary sense of the virus genome. There are no significant difference between HTN 76/118 and Howang strain on hydrophobicity of deduced polypeptides, but has slight difference on secondary structure.
Base Sequence ; Genome ; Hantaan virus* ; Hemorrhagic Fever with Renal Syndrome ; Hydrophobic and Hydrophilic Interactions ; Nucleotides ; Open Reading Frames ; Peptides ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length

We developed a sensitive, nested reverse transcription-polymerase chain reaction (RT-PCR) to detect Hantaan, Seoul, Belgrade, Puumala and Sin Nombre viruses in animal tissues. Total RNA was extracted from blood, lung or kidney samples of experimentally-infected hamsters by using the guanidine isothiocyanate buffer-acid phenol-chloroform method. Genus-reactive outer primers were derived from the consensus region of the G1 gene sequences of several hantaviruses. Serotype-specific primers were selected within the region amplified by the outer primers. To examine the sensitivity and specificity of the test, we diluted known quantities of Hantaan, Seoul, Belgrade, Puumala and Sin Nombre viruses in human or hamster immune sera before performing the nested RT-PCR. We could detect as little as 1 pfu of virus, even in the presence of high-titer neutralizing antibodies, and the serotype-specific primers amplified only homologous serotype viruses. RT-PCR with these primers demonstrated virus in the blood of experimentally-infected hamsters as early as four days to as late as 30 days after infection.4 comparison of a standard immunofluorescent antibody screening test (IFAT) to nested RT-PCR with RNA extracted from lung or kidney tissues of the hamsters, demonstrated that RT-PCR to be more sensitive for identifying viruses in these tissues.
Animals ; Antibodies, Neutralizing ; Consensus ; Cricetinae* ; Discrimination (Psychology)* ; Guanidine ; Hantavirus* ; Humans ; Immune Sera ; Kidney ; Lung ; Mass Screening ; RNA ; Sensitivity and Specificity ; Seoul ; Sin Nombre virus

No abstract available.
Child* ; Hemorrhagic Fever with Renal Syndrome* ; Humans

No abstract available.
Birds* ; Chiroptera* ; Hantavirus Infections* ; Hantavirus* ; Korea* ; Seroepidemiologic Studies*

No abstract available.
Animals ; B-Lymphocytes* ; Mice* ; Mitogens* ; Spleen*

No abstract available.
Animals ; Mice* ; Spleen*

No abstract available.
Animals ; Knee Joint* ; Knee* ; Mice* ; Parturition*

No abstract available.
Humans ; Male ; Military Personnel*

No abstract available.
Humans ; Male ; Military Personnel*

No abstract available.
Brain* ; Depression* ; Dexamethasone*