Objective To investigate the effect of perioperative fluid therapy on blood glucose in the neonates undergoing gastrointestinal operation. Methods Sixty-four ASA Ⅰ or Ⅱ neonates undergoing gastrointestinal operation were randomly divided into 4 groups ( n = 16 each). Group Ⅰ received water orally 2 h before operation and iv infusion of acetated Ringer's solution during operation. Group Ⅱ received water orally 2 h before operation and iv infusion of 2% glucose (in normal saline) during operation. Group Ⅲ received 10% glucose 5 ml/kg orally 2 h before operation and iv infusion of acetated Ringer's solution during operation. Group Ⅳ received 10% glucose 5 ml/kg orally 2 h before operation and iv infusion of 2 % glucose (in normal saline) during operation. Blood sam ples were collected from radial artery for determination of blood glucose concentrations at the beginning of fluid therapy (T0), immediately after induction of anesthesia (T1), at the beginning of operation (T2), and at 20,40and 60 min (T3-5) and 2 h after operation ( T6 ). MAP and HR were also recorded simultaneously. The blood lactic acid concentration was determined at T0 . Results There was no significant difference in MAP and HR among the 4 groups. Compared with group Ⅰ , blood glucose concentrations were significantly increased at T4-6 in group Ⅱand Ⅳ, but no significant change was found in blood glucose concentrations at each time point in group Ⅲ. There was no significant difference in blood glucose concentrations between group Ⅱ and Ⅳ. The blood lactic acid concentration was significantly higher in group Ⅲ than in group Ⅰ , and in group Ⅳ than in group Ⅱ . Conclusion Oral 10% glucose 5 ml/kg before operation and iv infusion of 2% glucose (in normal saline) during operation is beneficial for the stabilization of blood glucose levels in neonates undergoing gastrointestinal operation.

Studies have shown that the compliance of secondary prevention of ischemic stroke is not ideal. Among the factors that impact the compliance of secondary prevention, the correlation of the gender, age or disease severity has no clear conclusion. However, the married, the higher proportion of medical reimbursement, a better understanding of the disease, early intervention, the doctor's degree of attention, and a good doctor-patient relationship may improve the compliance of secondary prevention of ischemic stroke.

Objective To observe the clinical therapeutic effects of Yishen-Jianpi-Huoxue Decoction on the adolescent patients with Polycystic Ovary Syndrome (PCOS).Methods 42 patients diagnosed of PCOS of spleen and kidney deficiency syndrome were recruited in to traditional Chinese medicine group and treated with Yishen-Jianpi-Huoxue Decoction,and 35 patients of PCOS were recruited into control group and guided with the healthy lifestyle.The course of the treatment was 3 months.The menstrual cycle,body mass index (BMI),F-G score,GAGS score,basal body temperature (BBT),and endocrine related inspection were analyzed before and after the treatment.Results The clinical symptoms and related laboratory tests were improved in both groups after the treatment,but the effects of traditional Chinese medicine group was much better than the control group in BMI(23.11+3.64),GAGS score(7.33±7.06),FINS(14.73+8.97) mmol/L,and HOMA-IR (3.13 ±1.94),P＜0.05 or ＜0.01.Conclusion Yishen-Jianpi-Huoxue Decoction can improve the clinical symptoms and endocrine condition of edolescent PCOS patients.

Ecological system of subjects innovation is a strategic paradigm proposed by researchers of subjects innovation.In order to achieve efficient knowledge reuse and continuous knowledge innovation,Ecological system of subjects innovation reconstructs,integrates and optimizes the internal and external resource scattered in different subjects,knowledge chains ,knowledge alliances,knowledge clusters.To construct the cooperative innovation model of ecological system of subjects innovation.In this paper,the definition of ecological system of subjects innovation has been given.Then cooperative innovation model has been constructed and ecological system of subjects innovation has been demonstrated through some cases.Cooperative innovation model of ecological system of subjects innovation is composed of the purpose,the partner and the cooperation.Using extended network cooperation model,ecological system of subjects innovation gain the advantages of competition.

Nonvalvular atrial fibrillation (NVAF) is the most common type of arrhythmia and an independent risk factor for ischemic stroke.Warfarin effectively decreases the risk factors for ischemic stroke in patients with NVAF,but substantially increases the risk for severe bleeding.Currently,most clinical guidelines recommend using the CHADS2 score for stroke risk stratification in NVAF patients to guide antithrombotic treatment.Although its application is simple and convenient,the CHADS2 score has been debated because of many limitations; especially some common stroke risk factors were not included.By comparison,the CHA2 DS2-VASc scores have included more risk factors for stroke.It can better identify the truly low-risk patients.Among the AF patients with CHADS2 score of 0 to 1,CHA2DS2-VASc scores may provide the important information for stroke risk,and thus help the decision-making of the anticoagulant therapy.

AimTo realize the regulatory actions of nitric oxide(NO) on supraoptic nucleus (SON) neurons by observing the effects of NO donor sodium nitroprusside(SNP) on SON neurons. Methods The cell electrophysioloical properties were obtained by the intracellular recording techniques from the SON neurons in adult rat hypothalamic slices. Results In 11 silent cells, superfusion of SNP(1 mmol · L-1) for 3～5 min resulted in the depolarization response with a decrease of membrane resistance and time constant(P＜0.05) in 55% of cells, while hyperpolarization with an increase of membrane resistance in 45 % of neurons. In 73% of tested cells, SNP also decreased the amplitude, overshoot, firing frequency and slope of current-voltage curve, and increased the afterhyperpolarization of action potentials evoked by intracellular depolarizing current pulses(P ＜0.05).However, SNP enhanced the spontaneous firing in 67% of 6 tested cells with spontaneous activities. ConclusionSNP presents an inhibitory or excitatory effect on SON neurons in aneurontype-andfunctionalstate-dependent manner, which suggests that NO may exhibit adverse regulatory actions on SON neurons.

Relations between doctor and patient are basic and important human relation in medical practice.Because of some reasons,the relations appear tense status in our country,moreover the market economy flourishingly develops,as the result of medical organizations suffering more and more existent pressure.It is an important subject faced for people,especially for medical worker,to righly think the existed defects,and set up the mutual action between doctor and patient.

Cardiac muscles of mouse embryos, newborn and suckling mice were used for histological and histochemical studies. This paper presents the observations on the morphogenesis and ultrastructure of cardiac myocytes under electron microscope and on their reactions of nucleic acids (DNA and RNA), glycogen, lipids, succinodehydrogenase (SDH), alkaline and acid phosphatases (AKP and AcP), glucose-6-phosphatase (G-6-Pase) and adenosine triphosphatase (ATPase) under light microscope.The heart of mouse embryo before 12-day, contained numerous polygonal or star-shaped myoblasts which had not yet acquired myofibrils but a few myofilaments. As the myoblast developed, the number of myofilaments increased in number and formed myofibrils, then the cells became myocytes. By the end of embryonic period, all the special elements of myocyte were basically constituted. The myocytes of embryos were rich in RNA granules, and their DNA was deeply stained. Flourishing mitosis appeared only in the early embryonic phase. RNA of adult myocytes was much less than that of embryos. From the early phase of embryos myocytes were full of glycogen but short of lipid droplets. From the day it was born, glycogen decreased apparently but lipid droplets increased rapidly.The reaction of SDH steadily increased in intensity from its early phase to late one. After birth it became more intensive. G-6-pase first appeared in the myocytes of 14 day's embryos. In the fetal period it showed moderate positive reaction, but in the myocytes of suckling muose it appeared negative. The enzyme showed positive reaction again at the age of 2 weeks. The ATPase reaction was found to be weak in the fetal specimens, only appeared in the endothelium of the capillaries. After birth it gradually became intensive and from the 2nd week positive reaction was obvious in adult, it was very vigorous.The above observations showed that the embryonic development and differentiation were gradually completed. Histological and histochemical features of each developmental period showed their individualities, which confirmed the evidence that the cardiac muscle developed not only successively but also by stages, and approached adult's level at 2nd week end after birth.