TCM service trades grew rapidly and enjoyed optimistic future during the 12th five-year period, which was in good development trend. However, it is still in its primary stage of development and facing various challenges. This article argued that the development of TCM service trades will meet great opportunities, such as governmental policy encouragement, cooperation with other industries, the increasing acceptance of international market and the acceleration of internationalization of TCM. Meanwhile, there are also some difficulties - small industry scale and weak brand management, absence of legislation, lack of qualified professionals and various service trade barriers.

This summary is aimed to study the virtue,operative technique and curative effect for lumbar intervertebral disc herniation (LIDH) using self-fixation intervertebral disc endoscopy. A total of 520 LIDH patients accomplishing nucleotomy with self-fixation intervertebral disc endoscopy were adopted in the experiment,including 340 males and 180 females. Their courses of disease arranged from 4 months to 10 years (17.6 months average),and follow-ups for 4-12 months. The curative effect was evaluated according to uniform standard. The curative effects were excellent in 455 cases,good in 53 cases,poor in 10 case,and the better effect rate reached 98.2%. Dura mater laceration and cerebrospinal fluid leak happened in 3 cases,coccygeal nerve damage resulting in leg numbness appeared in 1 case. Those cases all received operations at the early stage,and there were no similar complication or infection presenting in intervertebral gap. Self-fixation intervertebral disc endoscopy has the characteristics of mini-invasion,handling facility and quick postoperative recovery,so as to be suitable for the treatment of single segmental LIDH. Additionally,it plays an impact on the cases with local spinal stenosis,osteophyma hyperplasia,lateral crypt constriction and nerve canal constriction.

Among all the road traffic injuries(RTI ),limb and spine fractures have a very high inci-dence.They can be caused by a variety of traumatogenic mechanisms,of whi ch crash is the most common.The distribution of types of fracture in RTI assumes a centrifugation-like p attern.Multiple fractures,homola teral limb injuries and violence-conducted in juries are common.The priority in RTI emergency is to treat serious concu rrent injuries and life-threatening comp lications.Only after the overall condition of the victim get stabilized can open injuries and fractures be treated ac tively.To treat victims of fracture s caused by RTI,especially those with a poor overall condition or an old age,appl ication of external fixation,espec ially a unilateral one,is a good consider-ation.And application of lockable i ntramedullary nailing to treat long tubular bone fractures has proved ve ry satisfactory.As for pelvis fractur es caused by RTI,external fixation c an be used initially while massive hemor-rhage resulted from the injury shoul d be treated vigorously.Internal fixation should be adopted as soon as th e vital signs get stabilized.To treat spine and spinal cord injuries,titanium a lloy is the first choice for fixation material.In most cases,anterior cervical fix ation and fusion with steel plate and screw is advisable for treatment of f ractures of cervical spine,while posterior i mmobilization with screw through pedicle of vertebra is usually suggested for treatment of fracture-dislocation of thoracolumbar vertebrae.

Objective:To investigate the effects of human factor Ⅸ (FⅨ) intron 1 inserted in forward orientation into retroviral vector on the expression of FⅨ in muscle cells. Methods: Two FⅨ mini-genes, FⅨm1 and FⅨm2, were inserted in forward orientation into retroviral vector backbone, LⅨSN, constructing 2 vectors named LⅨm1SN and LⅨm2SN, containing shortened intron of 1.4 and 0.3 kb, respectively. Retrovirus was made by transfecting PA317 packaging cells. Transient and stable expression of FⅨ was tested in SCID mouse muscle cells (SCID-MB). Results: The vector titers and expression levels of LⅨm1SN and LⅨm2SN in SCID-MB were 1 to 2 folds higher than that of LⅨSN. Some SCID-MB colonies transfected with LⅨm2SN contained intact FⅨm2 inton elements, suggesting that splicing of virus mRNA may not be complete in packaging cells. Conclusion: FⅨ intron 1 inserted in forward orientation in retroviral vectors can increase retroviral titer and the expression level of FⅨ in muscle cells.

Objective To measure the size and area of the vestibule of normal inner ear to provide basic data for further study of the vestibule-related diseases.Methods Two hundred forty patients (480 ears) who underwent temporal bone 64 slices CT scans were enrolled in this study from April 2011 to July 2012.These patients were divided into 4 groups,including Child group (＜ 18 years),Youth group (18-44 years),Adult group (45-59 years),and Elder group (≥ 60 years).The size and area of the vestibules were measured on the multiplanar reformatted images in post-processing workstation.Results There were no statistically significant differences in the size and area of vestibules in sides,genders and among the different groups (P ＞ 0.05),while there were statistically significant differences of those in different positions (P ＜ 0.05).Conclusion The measurements in different planes were as follow:1) In axial plane,the length of vestibule and its 95％ confidence interval were (5.89 ± 0.34),5.22-6.56 mm,respectively; the width (2.47 ± 0.36),1.76-3.18 mm; the area(13.97 ± 1.71) 10.62-17.32 mm2 2) in coronal plane,the length of vestibule and its 95％ confidence interval were (5.10 ± 0.32),4.47-5.73 mm,respectively; the width (2.42 ± 0.21),2.01-2.83 mm; the area (11.30 ± 1.33),8.69-13.91 mm2 ;3)in sagittal plane,the length of vestibule and its 95 ％ confidence interval were(5.27 ± 0.33),(4..62-5.92) mm,respectively; the width (3.72 ± O.35),(3.03--4.41) mm; the area (13.77 ± 1.55),(10.73-16.81)mm2.The volume CT combined with post-processing technology can accurately measure the size and area of the vestibules.The data can provide quantitative basis for diagnosis of the vestibule-related diseases.

Objective To investigate the clinical efficacy of warm needling moxibustion in treating peptic ulcer.Methods Sixty patients with peptic ulcer were randomly allocated to treatment and control groups, 30 cases each. The treatment group received warm needling moxibustion and the control group took omeprazole and ranitidine. The clinical therapeutic effects were compared between the two groups after two months of treatment.Results The cure rate and the total efficacy rate were 53.3% and 93.3%, respectively, in the treatment group and 33.3% and 76.7%, respectively, in the control group; there were statistically significant differences between the two groups (P<0.05). The efficacy rates for symptoms (gastralgia, stomach fullness, belching and acid regurgitation, and nausea and vomiting) were 93.3%, 89.3%, 88.5% and 91.7%.Conclusion Warm needling moxibustion is an effective way to treat peptic ulcer.

1 or 2 grade FL followed by a diffuse large cell lymphoma(DLCL) ot a Burkitt/Burkitt-like lymphoma is TL.TL maintains a phenotype suggestive of germinal center derivation.The most common immunophenotype is the same as that of FL, CD+10/bcl-6+. Obtaining a biopsy of TL is enhanced if the biopsy is directed to the site with the greatest SUV. The risk of transformation of about 30% at 10 years after the initial diagnosis of FL.The median duration of survival after transformation generally ranging from 1 to 2 years.HDCT-ASCT, allogeneic tranplantation,radioimmunotherapy and bendamustine are the possible therapy for TL.

BACKGROUND: Endothelium damage is the promoter of atherosclerosis, and endothelial progenitor cells as vascular endothelium precursor cells play an important role in endothelium repair and angiogenesis. The influence of statins on atherosclerosis through endothelial progenitor cells is the new research hot spot. OBJECTIVE: To summarize the research progress of statins through endothelial progenitor cells on atherosclerosis. RETRIEVAL STRATEGY: A computer-based search of Pubmed and Ovid database from January 2000 to December 2006, with the retrieval word of "endothelial progenitor cells, statins, atherosclerosis", and language was limited in English. Meanwhile CNKI and VIP database were retrieved from January 2000 to December 2006, the retrieval words were "endothelial progenitor cells, atherosclerosis, statins" in Chinese. Totally 62 articles were retrieved and checked primarily. Inclusive criteria: ①articles closely related to the influence of statins through endothelial progenitor cells on atherosclerosis;②articles published recently or in authorities journal were preferred in the same field. Exclusive criteria: repetitive research. LITERATURE EVALUATION: The mainly resource of documents is the influence of statins on atherosclerosis through endothelial progenitor cells. All 28 included documents were clinical or basic empirical studies, and 2 were reviews. DATA SYNTHESIS: Under physiological conditions, the integrity of the endothelial monolayer can be maintained by reproduction of adjacent cells. However, in conditions of oxidative stress damage, the integrity of the endothelium is prevented by progenitor cell homing.Statins mobilize endothelial progenitor cells to repair impaired endothelium and then postpone atherosclerotic progress. Statins mobilize endothelial progenitor cells through PI3K/Akt pathway and suppress endothelial progenitor cell apoptosis, leading to increase the number of endothelial progenitor cells in blood circulation, precipitate endothelial progenitor cells homing to myocardial ischemia site, promote new vascular generation, ameliorate blood supply of ischemia myocardium, and ameliorate the function of ventricular contraction. CONCLUSION: Through mobilizing endothelial progenitor cells, statins can increase the number of endothelial progenitor cells in blood circulation and suppress endothelial cells apoptosis, so as to postpone the progress of atherosclerosis, decrease the mortality rate of atherosclerosis patients, and improve their prognosis, which all indicate a wide research prospect.

Objective To observe the effect of Changtong Oral Liquid (CTOL) on serum TNF-? and IL-4 levels in rats with postoperative intestinal adhesion. Methods Sprague-Dawley (SD) male rats were randomly divided into six groups: normal control, no treatment control, Simo Oral Liquid (SOL) group, and CTOL group (minimal, medium and maximal dosages). Except for normal control group, intestinal adhesions were produced in all the other rats with the method of Ellis. The blood samples of rats with CTOL medium dosage and no treatment group were collected on the 1st, 3rd, 5th, 7th and 9th day, and that of the rest was drawn on day 7 after surgery for the determination of TNF-? and IL-4 levels. Results The serum TNF-? levels were higher in no treatment rats compared with the normal controls (P0.05). Conclusions TNF-? was closely related to adhesion formation, and it may be regarded as a marker of adhesion formation.

Objective To find the best level of transducer when continuous invasive arterial blood pressure monitoring using evidence-based approach. Methods Searched in three databases (Cochrane, PubMed,sinoMED), meanwhile the website of related academic societies,supplemented with Citation Index. Results There were 11 articles after selected. The majority of evidences (9 articles) suggested leveling with the heart, one suggested leveling with the catheter tip,and the other one regarded the matter as nothing important. Conclusions Transducer should be leveled to align the heart rather than the tip of arterial catheter when continuous invasive arterial blood pressure monitoring, but it doesn't matter which surface landmark of the heart. It was suggested that changing the level of transducer when changing the patient position or head of bed.