Objective To investigate effect of dicoumarol on the expression of serum tumor necrosis factor-α( TNF-α) in patients with acute cerebral infarction.Methods Eighty cases patients with acute cerebral infarction were admitted and randomly divided into two groups by digital draw:control group and observation group, 40 cases in each group.The control group were given conventional therapy for treatment of acute cerebral infarction, and observation group were given dicoumarol thrombolysis on the basis of control group.The serum TNF-αlevel and neurologic impairment score were compared between two groups pre-and post-treatment.Results After treatment of 6 h, the serum TNF-αlevel in observation group was significantly lower than that in control group (P<0.05).After treatment of 24 h, 48 h and 30 d, there were no significant differences of serum TNF-αlevels between two groups.After treatment of 5 d, 14 d and 28 d, the neurological deficit scores in observation group were significantly higher than those in control group (P<0.05).Conclusion Dicoumarol could effectively reduce TNF-αlevels in serum and extent of cerebral cell damage in patients.

Of the 411 hepatitis with severe jaundice admitted, 124 ( 30. 17%) had head perspiration. Based on Zhang Zhongjing's etiologic - pathogenic conception, 102 cases of such patients were treated by the principle of cooling and activating blood, benefitting Qi and Yin. 89 cases had jaundice eliminated, 13 cases had residual jaundice. All 22 cases of chronic severe hepatitis survived. Of them, 9 early stage cases were all survived, 7 of the 8 cases of intermediate stage survived, only 1 out of 5 cases of late stage survived.

Deubiquitylases remove ubiquitin moieties from different substrates to regulate protein activity and cell homeostasis .Since this posttranslational modification plays a role in several different cellular functions ,its deregulation has been associated with different pathologies .Aberrant expression of Ubiquitin -Specific Peptidase 22(USP22)has been associated with poor cancer prognosis .This article reviews the research status of USP 22.

Objective To investigate the changes of serum C reactive protein (CRP), Interleukin-2 (IL-2), Monocyte chemoattractant protein-1 (MCP-1) and hemorheology in patients with pelvic inflammatory disease. Methods Eighty patients with pelvic inflammatory disease were enrolled as observation group from May 2013 to May 2015, 60 healthy women in the same age were selected as control group at the same period. The serum CRP, IL-2, MCP-1 and hemorheology were compared between observation group and control group;the detection indexes were compared in patients with mild, moderate and severe pelvic inflammatory disease in observation group. Results The serum levels of CRP and MCP-1 and hemorheology of observation group were higher than those of control group, the differences were statistically significant (P<0.05) . The detection indexes of patients with severe pelvic inflammatory disease were higher than those of mild and moderate pelvic inflammatory disease, the detection indexes of patients with moderate pelvic inflammatory disease were higher than those of mild pelvic inflammatory disease, the differences were all statistically significant (P<0.05) . The level of IL-2 of was significantly lower than that of control group, the level of IL-2 of patients with severe pelvic inflammatory disease was lower than that of mild and moderate pelvic inflammatory disease, the level of IL-2 of patients with moderate pelvic inflammatory disease was significantly lower than that of mild pelvic inflammatory disease, the differences were all statistically significant ( P<0.05) . Conclusion The serum levels of CRP, IL-2, MCP-1 and hemorheology of patients with pelvic inflammatory disease have significant changes, and the detection indexes of patients with mild, moderate and severe pelvic inflammatory disease have significant differences.

Objective To probe the methods of experimental research for the drug activities of the rat prostates. Methods With androgen and Staphylococcus aureus injected into the prostates, the bacterial prostatitis (BP) models and benign prostatic hyperplasia with bacterial prostatitis (BHP-BP) models were developed. The animals were fed with tetracycline and then the drug activities in the serum and the prostatic tissues, the quantities of the bacteria and the histopathlogical changes in the prostateic tissues were separately detected in different time. ResultsAfter ten days, the histopathlogical changes of the benign hyperplasia and mesenchyma were found in the prostatic tissue from BHP animals and acute suppurative inflammation were dicegnosed in prostatic tissue from the BP group after infected for two days. The drug activities of 22.44 mg/g in BP group and 20.26 mg/g in BPH-BP group were found until 3.5～4 hours and the values were higher than MBC of the tetracycline and the antibacterial activity of the sera at the same time. After 8 to 11 days of treating, the bacteria would not be isolated from the prostates of the animals with BP or BHP-BP. Stopping treatment a week 11 days after treating acute or chronic inflammation histopathlogical change did not be found in the prostates of animals with BP. 30 CFU of bacteria could be found in per gram tissue of prostate and both of the acute and chronic suppurative inflammation could be found in the BP group without treatment at the 28 days afterinfected.Conclusion The tetracycline activities in the prostates of rats with BP or BPH-BPis same as or even higher than that of serum and the MBC of the drug. The quantity of sensitive bacteria in prostates will be diminished and disappeared during treatment.

Objective To clone the full-length cDNA of rat B cell lymphoma-2(bcl-2) gene,then construct and identify the cytomegavirus-mediated lentiviral expression vector of bcl-2 gene,and assess the gene expression in 293T cell,which is a human embryonic kidney cell line.Methods The full-length bcl-2-cDNA fragment was amplified by reverse transcription polymerase chain reaction(RT-PCR) from the kidney tissue of a Wistar rat.The double-stranded oligonucleotides(dsOligoe) were then cloned into the pMD18-T plasmid.After confirmation of a correct construction by sequencing,the positive clone was subcloned into pGC-FU vector with enhanced green fluorescent protein(EGFP),and then transformed into DH5a competent cells.The restricted endonuclease and T4 DNA ligase were used to construct the lentiviral expression vector plasmid pGC-FU-bcl-2 which,combined with the lentiviral packing materials(pHelper 1.0,pHelper 2.0),was then transfected into 293T cell line to form the recombinant lentivirus pGC-FU-bcl-2,and it was used to transfect the 293T cells.The expression of pGC-FU-bcl-2 was further verified by detecting EGFP and bcl-2.Results 1) It was verified by DNA sequencing that the sequence of rat bcl-2 gene was consistent with reported sequence in GenBank.2) The bcl-2 gene was successfully combined in pGC-FU-bcl-2 recombinant plasmid which could be transfected into human embryonic kidney cells.3) The recombinant virus pGC-FU-bcl-2 could be obtained from the 293T cells by co-transfection of pGC-FU-bcl-2 and packing plasmids.4) Targeting gene could be cloned into 293T cells by the recombinant lentivirus with steady expression.The fluorescent protein could be observed under microscope and the expression of bcl-2 protein was detected by Western blotting.Conclusions The lentiviral expression vector containing EGFP and bcl-2 gene has been successfully constructed,with which the transfected 293 T cells can lead to a steady expression of bcl-2 protein.The present study provides a basis for the further study of the function of bcl-2 gene and a potential therapy for the diseases relating to apoptosis.

Objective To analyze and discuss the utility of parenteral nutrition in inflammatory bowel disease (IBD).Methods In the light of the diagnostic criteria of IBD by the National Conference in 1993 as to the chronic noninfectious bowel disease,the inpatients of IBD in our department from Jan.1986 to Dec.1996 were analyzed,and the medical treatment of IBD and the role of parenteral nutrition evaluated.Results It was demonstrated that the annual average number of hospitalized patients had increased in the past 11 years,and the parenteral nutrition resulted in a better outcome,in addition to SASP or 5-ASA.Conclusion It was believed that following the principal treatment of IBD, nutritional support is also essential.

Aminoacyl-tRNA synthetase catalyzing the first reaction of protein biosynthesis. In mammalian cells, eight aminoacyl-tRNA synthetases (aaRSs) and three auxiliary protein factors form a macromolecular aminoacyl-tRNA synthetases complex (aaRS complex). The three nonsynthetase protein factors, namely, p43, p38, and p18 were found to be involved in many other important life activities besides their roles in the complex. The auxiliary factor p43 was the precursor of endothelial monocyte activating polypeptideⅡ (EMAPⅡ), which involved in angiogenesis and apoptosis. The auxiliary factor p38 was crucial for the development of lung, and its abnormal accumulation in neuron would be related to the Parkinson’s disease. The auxiliary factor p38 and p18 could promote the repair of DNA damage via different pathways in a highly organized way. All these breakthroughs enhance our understanding about the interaction between the aaRS complex and the macromolecular signaling network and promote the studies on this field.

Through the analysis on principles of dental panoramic radiography, we analyzed the influence factors of the dental arch, and provided the main principles for optimizing design to improve the image quality. Through the analysis on the human dental arch’s form, we optimized the radiographic procedure. Many factors affect the quality panoramic image, but the focal trough’s design is one of the most important factors, especially for canine regions. Optimizing design of focal trough can lessen degrees of horizontal and vertical magnification, also can reduce geometric distortion and overlapped images of teeth.

Objective] Make a research on curative properties from the aspect of zang-fu differentiation in dysuria of Zhang Zhong-Jing. [Methods] Detailly discussed about the properties of dysuria from five aspects: lung, spleen, kidney, triple energizer and bladder. Combine these factors with articles in Treatise on Exogenous Febrile Disease to estimate the condition of body fluid and prognosis of the disease. [Results]The several methods to diagnose and treat dysuria from the idea of Zang-fu Theory, such as ventilating lung and relieving the exterior, invigorating spleen and dissipating excessive fluid, purging and benefiting triple energizer, warming kidney and transforming qi, promoting urination and activating yang. Smooth or not of urine could help to locate the lesion viscera, determine the attribute of deficiency and excess of etiology and judge the prognosis of diseases. [Conclusion]Zhang Zhong-Jing ’s Zang-fu syndrome differentiation theory is an effective way to diagnose and cure dysuria in clinical practices, it is worthy of further research.