Aim To investigate the effects of com-bined treatment of SAHA and TRAIL on human breast cancer ER positive cell line MCF-7 . Methods MCF-7 cells were treated with SAHA and/or TRAIL. The inhibitory rates were detected by real-time cell prolifer-ation assays. Morphology changes of MCF-7 cells were observed through time-lapse live cell imaging acquisi-tion. Results Real-time cell proliferation assays showed that the anti-tumor efficacy of SAHA was sig-nificantly enhanced in combination with TRAIL. The results of time-lapse live cell imaging acquisition dem-onstrated that, with treatment of SAHA and TRAIL, the growth inhibition of MCF-7 cells was more obvious than that of in TRAIL or SAHA treatment alone. Con-clusion The combination treatment of SAHA and TRAIL has a synergistic effect of growth inhibition on breast cancer MCF-7 cells.

Objective To analyze the effects of gentongping pill combined with physiotherapy on the patients with vertebral artery type of cervical spondylosis. Methods 134 patients with vertebral artery type of cervical spondylosis were grouped into two groups, the observation group and the control group. The two groups were treated with physiotherapy and the observation group was treated with Gentongping pill. The effects in the two groups were evaluated. Results The total efficiency in the observation group was higher than that in the control group(P<0.05). After treatment, the symptom score, CASCS score, mean blood flow velocity in left vertebral artery and right vertebral artery, TNF-α and IL-6 level in the observation group were better than those in the control group (all P<0.05). There was no obvious adverse reaction in the two groups. Conclusion Itcan improve the therapeutic effect which Gentongping pill is used on the treatment of patients with vertebral artery type of cervical spondylosis.

The medical equipment benefit evaluation analysis has become an important constituent of cost and medical equipment management. It can not only use the economic benefit index to estimate the service condition of equipment, but also form the comprehensive evaluation system with basic of economic benefit evaluation and auxiliary of science study. Based on comprehensive evaluation result, to search the weakness of equipment utilization and promote the overall development of hospitals and clinics orderly.

Based on the imaging photoplethysmography (iPPG) and blind source separation (BSS) theory the author put forward a method for non-contact heartbeat frequency estimation. Using the recorded video images of the human face in the ambient light with Webcam, we detected the human face through software, separated the detected facial image into three channels RGB components. And then preprocesses i.e. normalization, whitening, etc. were carried out to a certain number of RGB data. After the independent component analysis (ICA)'theory and joint approximate diagonalization of eigenmatrices (JADE) algorithm were applied, we estimated the frequency of heart rate through spectrum analysis. Taking advantage of the consistency of Bland-Altman theory analysis and the commercial Pulse Oximetry Sensor test results, the root mean square error of the algorithm result was calculated as 2. 06 beat/min. It indicated that the algorithm could realize the non-contact measurement of heart rate and lay the foundation for the re- mote and non-contact measurement of multi-parameter physiological measurements.
Algorithms ; Face ; Heart Rate ; Humans ; Monitoring, Physiologic ; methods ; Oximetry ; Photoplethysmography ; Software ; Video Recording

The multilateral trading system embodied in the World Trade Organization has contributed significantly to economic growth, development and employment throughout the past fifty years. We are determined, particularly in the light of the global economic slowdown, to maintain the process of reform and liberalization of trade policies, thus ensuring that the system plays its full part in promoting recovery, growth, and development. The negotiations on trade in services shall be conducted with a view to promoting the economic growth of all trading partners and the development of developing and least-developed countries. The General Agreement on Trade in Services (GATS) is among the World Trade Organization's most important agreements. The accord, which came into force in January 1995, is the first and only set of multilateral rules covering international trade in services. Ig has been negotiated by the Governments themselves, and it sets the framework within which firms and individuals can operate. The GATS has two part: the framework agreement containing the general rules and disciplines ; and the notional "schedules" which list individual countries' specific commitments on access to their domestic markets by foreign suppliers. Each WTO Member lists in its national schedule those services for which it wishes to guarantee access to foreign suppliers. All commitments apply on a non-discriminatory basis to all other Members. There is complete freedom to choose which services to commit. In addition to the services committed, the schedules limit the degree to which foreign services providers can operate in the market. For example, a country making a commitment to allow foreign banks to operate in its territory may limit the number of banking licenses to be granted (a market access limitation). It might also fix a limit on the number of branches a foreign bank may open (a national treatment limitation). The GATS covers all internationally-traded services with two exception : services provided to the public in the exercise of governmental authority, and , in the air transport sector, traffic rights and all services directly related to the exercise of traffic rights and all services directly related to the exercise of traffic rights. The GATS also defines four ways in which a service can be traded, known as "modes of supply" Services supplied from one country to another (e.g international telephone calls), officially known as "cross-border supply"; Consumers from one country making use of a service in another country(e.g tourism), officially known as "consumption abroad"; A company from ton country setting up subsidiaries or branches to provide services in another country (e.g a bank from one country setting up operations in another country), officially known as "commercial presence" ; and Individuals travelling from their own country to supply services in another (e.g an actress or construction worker), officially known as "movement of natural persons". Trade liberalization, and even economic growth, are not the ends in themselves. The ultimate aim of Government is to promote human welfare in the broadest sense, and trade policy is only one of many instruments Governments use in pursuing this goal. But trade policy is nevertheless very important, both in promoting growth and in preventing conflict. The building of the multilateral trading system over teh past 50 years has been one of the most remarkable achievements of international cooperation in history. The system is certainly imperfect, which is one of the reasons why periodic negotiations are necessary, but the world would be a far poorer and more dangerous place without it. In January 2000, WTO Member Governments started a new round of negotiations to promote the progressive liberalization of trade in services. The GATS agreement specifically states that the negotiations "shall take place with a view to promoting the interests of all participants on a mutually advantageous basis" and "with due respect for national policy objectives and the level of development of individual Members". The pace and extent of these negotiations are set by the WTO's 140 Member Goverments themselves according to their different national policy priorities. Recently, however, the negotiations and the GATS itself have become the subject of ill-informed and hostile criticism. Scare stories are invented and unquestioningly repeated, however implausible. It is claimed for example that the right to maintain public services and the power to enforce health and safty standards are under threat, though both are explicitly safeguarded under the GATS. How have serious people come to believe what is, on the face of it, out of the question? Why should any Government, let alone 140 Governments, agrees to allow themselves to be forced, or force each toher, to surrender of compromise powers which are important to them, and to all of us? Decision-making in open societies presupposes informed public discussion. It must be based on fact rather than fiction. The purpose of this booklet is to contribute to this discussion and to a greater public understanding of the GATS by correcting statements made in some recent publication which we believe are misleading the public and undermining support for international economic cooperation. It must not be assumed that becuase we have disputed some allegations we accept that others are well-founded: these are merely examples.
Appointments and Schedules ; Delivery of Health Care* ; Economic Development ; Employment ; Financing, Organized ; Freedom ; Humans ; International Cooperation ; Licensure ; Linear Energy Transfer ; Negotiating* ; Pamphlets ; Publications ; Telephone

A reversed passive hemagglutination technique in which the antibody of fibrinogen is used as a model and the tannic acid is used as a coupling reagent for the attachment of antihuman fibrinogen serum to erythrocytes is described. Various factors which influence the coupling of purified rabbit antihuman fibrinogen serum IgG were examined. A standard sensitizing procedure was deduced. It is simple and rapid, and can also be used for coupling of other proteins and testing their antisera and antigen. It is superior to passive hemagglutination inhibition test for estimation of FDP. Through laboratory observation and clinical use for two years, it is considered that this method is simple, sensitive and the results are reproducible. It provides a useful tool for the analysis of FDP.

Purpose To investigate the influence of interpersonal relationship sensitivity on anxiety and depression emotion and subjective well-being for young adults epileptic patients.Methods 87 young adults epileptic patients were divided into interpersonal relationship sensitive group ( study group) and normal group ( control group),according to sensitivity factor ≥ 3 in the symptom checklist(SCL-90).Self-rating anxiety scale ( SAS),self-rating depression scale (SDS) and general well-being scale (GWB) were then established and analyzed.Results The scores on interpersonal relationship sensitivity factor of the study group and control group were 3.64 ±0.51 and 2.73 ± 0.43,respectively,which had remarkable difference ( t =8.91,P ＜ 0.01 ).There were 47 patients with interpersonal relationship sensitivity among the 87 patients,accounting for 54.02％.The scores on SDS and SAS of the study group were higher than control group (P＜0.01 ).The scores on GWB of the study group was significantly lower than the control group (P＜0.01).The scores on GWB of the study group,reflecting the factors about health,energy,satisfaction and interest to life,depression and happy emotion,and relax and nerves,were lower than control group (P＜0.01) ; while the scores on emotion and behavior control were higher than the control group with minor difference (P ＞ 0.05 ).The scores on interpersonal relationship sensitivity factor and SDS,SAS and GWB were positively correlated except for O factor of GWB.Conclusion The level of interpersonal relationship sensitivity has important influence on anxiety and depression emotion and subjective well-being in young adults epileptic patients.

Circulating endothelial cells (CEC) are endothelial cells which are detected in the peripheral blood. There are very few CEC in healthy adults while the number is obviously increasing in patients with arthrosclerosis, diabetes mellitus, lupus erythematosus, et al. Nowadays, flow cytometry analysis and immunomagnetic isolation for CEC are employed successfully in clinic and scientific research. Several research findings have confirmed that there is intimate relation between CEC and tumorigenesis. Because of the important role in angiogenesis and tumor growth, CEC would be a perspective tumor marker in antiangiogenesis and would also predict the chemotherapy efficacy.

With the development of proteomic technologies, large numbers of putative biomarkers have been screened out but few of them are put into the clinical application.Recently, MRM-MS based approaches and strategies have aroused extensive concern in domestic and overseas clinical laboratory community because of their cost-efficiency, time-saving, high analytical sensitivity and accuracy.MRM-MS based approaches, especially SISCAPA, may gradually become a key technology for the protein biomarker research in the near future.In this review, the bottleneck existing in the field of protein biomarkers research, and features as well as applications of MRM-MS are discussed and the future trend of research is prospected.

MicroRNAs are a kind of small endogenous non-protein-coding single-stranded RNA,which are 18 to 25 nucleotides long.They either regulate expression of specific genes through the degradation of mRNA or repress expression of target protein of mRNA postoperation by binding with the 3' untranslated region of targeting mRNAs.It produces important physiological effects on cell proliferation, differentiation and apoptosis.The use of experimental biology and bioinformatics methods for research of tumor-associated miRNAs has become research focus.In this paper, the role of miRNAs in the oncogenesis, development, metastasis and prognosis of gallbladder cancer is mainly introduced.