The platelet membrance glycoprotein plays a key role in the adhesion,aggregation and release of platelet.It is the specific molecular marker for platelet activation.Many studies have confirmed that platelet membrane glycoprotein CD62P may be more sensitive and specific to reflect the occurrence of coronary artery development,CD62P level is positively correlated with the severity.A variety of traditional Chinese medicine can lower the CD62P level,inhibit platelet activation and the treatment play an important role in the treatment.

There exist a number of medical psychology teaching gaps in the present Chinese medicine institutions including the course nature,teaching content,course construction and what is in line with the requirement of examination outline for professional doctors. By changing medical psychology into the required course,increasing class time,fractionizing teaching content and carrying out case teaching and practice teaching can enhance Chinese medicine graduates’ability to understand and apply the principle of modern psychology and technology.

Objective To investigate the effect of knocking down miR-19a on proliferation and apoptosis of human glioma cell line U251 in vitro.Methods U251 cells were cultured routinely.MiR-19a inhibitor was transfected into U251 cells by Lipofectamine 2000.At the same time,the negative control group and blank control group were established.The expression level of miR-19a was detected by RT-PCR.and cell proliferation was analyzed by CCK-8 assay.The changes of cell apoptosis and cycle were monitored by flow cytometry.Results Compared with the negative control group and blank control group,qRT-PCR showed that the expression level of miR-19a was significantly reduced after transfection (F=124.72,P ＜ 0.01).CCK-8 revealed that proliferation ability of miR-19a inhibitor group was significantly suppressed.The cell survival rate in miR-19a inhibitor group after 48 h was (48.27-±8.23) ％,compared with the blank control group (100.00 ％),the difference was statistically significant (t =12.45,P ＜ 0.01).After low-expression of m iR-19a,the G0/G1 phase cells were increased and S phase cells were decreased.The low-expression of miR-19a could induce cell apoptosis.Conclusions Low-expression of miR-19a can inhibit cell proliferation,block G1/S transition and induce apoptosis in human glioma cell line U251.miR-19a may serve as an attractive target of gene therapy for glioblastoma.

Objective To use puncture and consultation system for improving the needle puncture success rate of pediatric nurses, upgrading the level of puncture technology, reducing patient complaints and increasing patient satisfaction. Methods 200 pediatric patients selected from March,2006 to March, 2010 were divided into the intervention group and the control group, The intervention group adopted puncture and consultation system while the control group did not. The success rate of puncture, patients complaints and patients satisfaction were compared. Results The puncture success rate of pediatric nurses increased,and patients' complaints reduced and patients' satisfaction degree increased in the intervention group compared with that of the control group. Conclusions The pediatric puncture and consultation system in the tertiary hospital not only increases the satisfaction degree of pediatric patients' parents, but also improves the level of puncture technique of pediatric nurses, and has important social effect outside hospital.

0.05).According to the water analysis for magnesium, the therapeutic trials with magnesium salts and the animal experiments, we suppose that there might be some myocardial necrotizing factors in magnesium deficiency in endemic districts.

Radiation-induced stomatitis is a frequent side effect for head and neck cancer patients undergoing radiotherapy, which affects both the treatment and the life quality. The direct effects of radiation, oxidative stress, transcription factor, proinflammatory cytokine and pathogenic microorganism are involved in the onset of radiation-induced stomatitis. The pathologic process can be divided into five phases including initiation, up-regulation, amplification, ulceration and healing. Understanding of the pathogenesis and risk factors provides basis for prevention and control of radiation-induced stomatitis.

Objective To investigate effects of hydrogen sulfide on hepatic ischemia-reperfusion injury in rats.Methods Thirty healthy male SD rats weighing 220-250 g were randomly divided into sham operation group (S group),ischemia-reperfusion group(IR group) and different doses of hydrogen sulfide groups (H2S1～3 groups),every group contains six rats.In S group hepatic portal was only exposed but not ligated.In IR group the left and middle lobes of hepatic pedicle,portal vein and hepatic artery were clamped for 1 h and then reperfuse.H2 S1～3groups received intraperitoneal injection of NaHS 14,28,56 μmol/kg at 5 min before reperfusion.Blood samples were collected for measuring the activity of alanine transaminase (ALT) and aspartate transaminase (AST) in serum at 6 h after reperfusion.Liver tissue samples were collected for measurement the glutathione (GSH) content and pathologic examination.Results Compared with S group,the activity of ALT and AST in serum was increased,GSH content in liver tissue decreased in IR group ; Compared with IR group,the activity of ALT and AST in serum was decreased,GSH content in liver tissue increased in H2 S1～3 groups.Hepatic tissue pathologic injury was reduced in H2 S1～3 groups as compared with IR group.Conclusion Hydrogen sulfide can reduce hepatic ischemia-reperfusion injury in rats.

Colorectal cancer is one of the most common cancer.Despite the survival rate of patients with metastatic disease has been raised due to the combination therapy of chemotherapy and opetation,few longterm survivors exist.The detection of circulating tumor cells (CTC) is an evolving technology whose significance has been discussed for more than a century.The advence of modem technology has allowed for more reliable detection of CTCs,and recent studies have provided compelling evidence that CTCs can predict clinical outcome in metastatic colorectal cancer (mCRC).In this review,we will discuss the evidence for CTC significance,with particular focus on detection methods,novel markers,and clinical outcomes in CRC,and future challenges in testing them as a means to individualize the therapy of mCRC patients.

Magnetic nanoparticle will become a kind of mean and colloidal solution if it is coated with surfacant.Magnetic nanoparticle has a wide clinical practice because it can produce heat in alternating magnetic fields,especially in malignant tumors thearapy.Magnetic nanoparticle hyperthermia,nano-delivery and magnetic target drug,and intravascular embolization with magnetic particle will bring new hope for tumor patients.

Objective To learn current status of elderly depressive disorders in Yuetan community residents to provide evidence for policy making.MethodsCluster sampling method was used to enroll 352 elderly individuals from Yuetan Community for Geriatric Depression Scale(GDS)and self-designed questionnaire.Results The morbidity of elderly depressive disorder was 23.86%(84/352).The awareness rate of risk factor of elderly depressive disorder Was 4.83%to 42.90%.Conclusion The morbidity of elderly depressive disorder of Yuetan Community is higher,and related healthcare education should be strengthened.