Objective To investigate the effect of knocking down miR-19a on proliferation and apoptosis of human glioma cell line U251 in vitro.Methods U251 cells were cultured routinely.MiR-19a inhibitor was transfected into U251 cells by Lipofectamine 2000.At the same time,the negative control group and blank control group were established.The expression level of miR-19a was detected by RT-PCR.and cell proliferation was analyzed by CCK-8 assay.The changes of cell apoptosis and cycle were monitored by flow cytometry.Results Compared with the negative control group and blank control group,qRT-PCR showed that the expression level of miR-19a was significantly reduced after transfection (F=124.72,P ＜ 0.01).CCK-8 revealed that proliferation ability of miR-19a inhibitor group was significantly suppressed.The cell survival rate in miR-19a inhibitor group after 48 h was (48.27-±8.23) ％,compared with the blank control group (100.00 ％),the difference was statistically significant (t =12.45,P ＜ 0.01).After low-expression of m iR-19a,the G0/G1 phase cells were increased and S phase cells were decreased.The low-expression of miR-19a could induce cell apoptosis.Conclusions Low-expression of miR-19a can inhibit cell proliferation,block G1/S transition and induce apoptosis in human glioma cell line U251.miR-19a may serve as an attractive target of gene therapy for glioblastoma.

Objective To investigate the curative efficacy of safflower yellow sodium chloride in the treat-ment of acute myocardial infarction (AMI)and its effects on cardiac function and oxidative stress.Methods 110 cases of AMI meeting the inclusion criteria were randomly divided into two groups equally.The control group was given conventional drug treatment and percutaneous coronary intervention (PCI),while the observation group was given con-ventional drug treatment and PCI in combination with safflower yellow sodium chloride.Then,the curative efficacy, indices of cardiac function and oxidative stress were observed and compared.Results The observation group had a total effective rate of 94.5%,which was significantly higher than 81.8% in the control group (χ2 =4.94,P =0.026).After the treatment,SV (t =3.23,P =0.002),CO(t =3.82,P =0.000)and LVEF (t =3.14,P =0.002) in the observation group were significantly higher than those in the control group (all P <0.05 ).As to oxidative stress,in comparison with the control group,SOD (t =8.42,P =0.000)was significantly higher and MDA (t =3.99, P =0.000)was significantly lower in the observation group (all P <0.05 ).Conclusion Adjuvant treatment of safflower yellow sodium chloride for AMI was effective,which could significantly increase curative efficacy,improve cardiac function and alleviate oxidative stress.

Airway remodeling is one of the main pathological changes of bronchial asthma. It has shown that a variety of cytokines, including chemokines, growth factors, matrix proteins, participate in and even strengthen airway remodeling through building complex signal transduction pathways. Many traditional Chinese medicines,such as shegan mahuang decoction,have a regulatory role in airway remodeling.

This paper pondered thoroughly the analysis of the mental status of earthquake victims,the preparation,operational measures and steps,and remarkable issues in post-earthquake psychological crisis intervention,in order to provide mental support for the post-earthquake reconstruction.

The quality of teaching staff is closely correlated with the development of students and educational career.Therefore,educational administrators should enhance their concepts of management,clarify requirement and quality of teaching staff under the circumstance of new curriculum reform,and explore effective approaches to enhancing the quality of teaching staff,so as to unceasingly improve the quality of teaching staff.

Objective To explore the effect of paravertebral nerve block for preemptive analgesia on patients with thoracic surgery,and provide reference for clinical anesthesia.Methods From March 2014 to February 2016,102 patients with thoracic surgery in our hospital were selected as the research object,and they were divided into the observation group and the control group with 51 cases in each group according to the anesthesia methods.All the patients were given total intravenous anesthesia.Patients of the control group were injected propofol 1~2 mg/kg,sufentanil 0.4~0.5 g/kg,midazolam 0.1 mg/kg and rocuronium 0.5~0.7 mg/kg,while patients of the observation group were injected ropivacaine(with concentration of 0.5%)5 mL,sufentanil 0.1~0.3 μg·kg-1·min-1 and propofol 6~8 mg·kg-1·min-1 as an addition with the use of ultrasound to guide the paravertebral nerve block.The effect of the two groups were compared.Results The dosage of propofol,remifentanil and rocuronium in the observation group were significantly lower than those in the control group during the operation.The VAS score,postoperative pulmonary complication rate,ICU rate,extubation time,postoperative recovery time at different time of observation group were significantly lower than those of the control group,and SpO2 was higher in control group 30 minutes after tracheal extubation, the differences were significant (P<0.05).Conclusion Paravertebral nerve block requires less dosage of anaesthetic, and it has more significant analgesia effect on patients with thoracic surgery, which can efficiently promote the recovery of patients.

Magnetic nanoparticle will become a kind of mean and colloidal solution if it is coated with surfacant.Magnetic nanoparticle has a wide clinical practice because it can produce heat in alternating magnetic fields,especially in malignant tumors thearapy.Magnetic nanoparticle hyperthermia,nano-delivery and magnetic target drug,and intravascular embolization with magnetic particle will bring new hope for tumor patients.

Objective To use puncture and consultation system for improving the needle puncture success rate of pediatric nurses, upgrading the level of puncture technology, reducing patient complaints and increasing patient satisfaction. Methods 200 pediatric patients selected from March,2006 to March, 2010 were divided into the intervention group and the control group, The intervention group adopted puncture and consultation system while the control group did not. The success rate of puncture, patients complaints and patients satisfaction were compared. Results The puncture success rate of pediatric nurses increased,and patients' complaints reduced and patients' satisfaction degree increased in the intervention group compared with that of the control group. Conclusions The pediatric puncture and consultation system in the tertiary hospital not only increases the satisfaction degree of pediatric patients' parents, but also improves the level of puncture technique of pediatric nurses, and has important social effect outside hospital.

Objective To learn current status of elderly depressive disorders in Yuetan community residents to provide evidence for policy making.MethodsCluster sampling method was used to enroll 352 elderly individuals from Yuetan Community for Geriatric Depression Scale(GDS)and self-designed questionnaire.Results The morbidity of elderly depressive disorder was 23.86%(84/352).The awareness rate of risk factor of elderly depressive disorder Was 4.83%to 42.90%.Conclusion The morbidity of elderly depressive disorder of Yuetan Community is higher,and related healthcare education should be strengthened.

Induced pluripotent stem cell ( iPS cell ) , similar to embryonic stem cell, can be repro-grammed to the pluripotent state by ectopic expression of specific transcription factors. The iPS cells have pluri-potency and can be induced into neuron cells,which represent a promising cellular tool to study human neurode-velopmental disease,drug screening,diagnosis and personalized treatment. This article reviews the latest progress on iPS cell and its applications in neural developmental disease.