Cholesteryl ester transfer protein(CETP) plays an important role in the transfer and exchange of cholesteryl esters,phospholipid and triacyglycerols.It affects lipid composition,concentration,particle size and function of lipoproteins, partcipating in the development of atherosclerosis.The role of CETP in human atherosclerosis has not been fully elucidated.Gene variations of the CETP were associated with altered CETP plasma levels and activity,lipid metabolism disorder.The relationships between CETP gene variation,lipid parameters and cardiovascular risk was reviewed.

High density lipoprotein(HDL) is an antiatherogenetic lipoprotein and low level of HDL-cholesterol(HDL-C) in plasma is a risk factor for coronary heart disease.Therefore,raising the level of HDL-C by pharmaceuticals,oestrogen replacement and biologic therapy for dyslipidemia exhibit great clinical significance.

Objective To investigate the expression level of serum miR-193a-3p,miR-337-5p and miR-483-5p in esophageal squamous cell carcinoma (ESCC) patients and to explore their value for diagnosis and prognosis of ESCC.Methods Serum samples were collected from 63 ESCC patients before and after surgery in Nanjing General Hospital and Xuzhou Cancer Hospital between June 2013 and May 2014 and serum sanples of 63 age-and sex-matched healthy individuals acted as the normal controls.TaqMan Low Density Assay was used to detect the deregulated miRNA in ESCC patients and then quantitative real-time PCR was used to validate the upregulated miRNA miR-193a-3p,miR-337-5p and previously reported miR483-5p that was upregulated in oral squamous cell carcinoma (OSCC).Finally,the three miRNA were evaluated for their clinical value in the diagnosis and predicting prognosis of ESCC.Results Compared with normal controls,serum levels of miR193a-3p,miR-337-5p and miR-483-5p in ESCC patients were significantly up-regulated (0.459±0.339 vs 0.195±0.084,U =591;5.686±5.211 vs 2.476±0.808,U=605;32.545 ± 22.479 vs 19.509±10.601,U=1 037,respectively,all P＜ 0.0001) and their levels were significantly reduced after the surgical treatment (P＜0.05).The areas under the ROC curve of serum miR-193a-3p,miR-337-5p,miR-483-5p and miR-Panel were all larger than that of CEA.Univariate Kaplan-Meier analysis revealed that ESCC patients with low expression level of miR-483-5p in postoperative serum exhibited higher survival rate than those with high level(P=0.022).Conclusion Serum miR-193a-3p,miR-337-5p and miR-483-5p can be potential molecular biomarkers in the diagnosis and predicting prognosis of ESCC.

Objective:To investigate the possible interaction of Lp(a), and Lp(a) circulating immune complexes (CIC) 〔Lp(a)-CIC〕 level with triglyceride (TG). Methods:Plasma Lp(a), Lp(a)-CIC and LDL-CIC levels were determined by ELISAs in 232 patients with various dyslipidaemias, respectively. Results:Hypertriglyceridaemic patients exhibited the lowest plasma Lp(a) levels, while hypercholesterolaemic patients exhibited the highest. Patients with mixed hyperlipidaemia had intermediate serum Lp(a) concentrations, which were significantly lower than those in the controls. Interestingly, we also found that hypertriglyceridaemic patients exhibited the lowest plasma Lp(a)-CIC and LDL-CIC levels, while hypercholesterolaemic patients exhibited the highest. TG levels were negatively correlated with Lp(a) (r=-0.15, P

Human serum paraoxonase (PON1) is a high density lipoprotein (HDL)associated enzyme capable of hydrolyzing lipid peroxides in vitro .PON1 has recently attracted attetion as a key factor against oxidative modification of LDL by involving in degradation of oxidized phospholipid and may therefore play an important role on the prevention of the atherosclerotic process of HDL.The concentration and activity of PON1 have been shown to be associated with the risk of atherosclerosis.In addition,the genetic polymorphisms of PON1 have been proposed as a genetic marker of risk for coronary artery disease.The measurement of the genetic polymorphisms of PON1 may be helpful in the prevention of coronary artery disease.

Objective:To purify apolipoprotein H (apoH) from fresh human serum with heparin affinity column self-prepared and to prepare polyclonal antibody anti-ApoH.Methods:Affinity column was made by reaction of indirect reductive amination and operating conditions were optimized and their effects were investigated.The apolipoprotein H antigen was purified from human serum by methods of perchloric acid precipitation,ion exchange and affinity chromatography.The polyclonal antiserum against the antigen was obtained by immuning rabbits.Results:The amount of heparin immobilized on the synthesized gel was 5 mg/ml (medium).Molecular weight of the purified antigen was about 50 kD by SDS-PAGE and no other bands were seen,the percentages of 16 amino acids were consistent with that reported previously.In ELISA and dot-ELISA,the polyclonal antibody displayed a cross reaction with bovine serum albumin,while no such reaction was observed in result of Western blot under reduced and denatured condition.Conclusion:The self-prepared affinity column costs very low,but has excellent affinity performance and stable heparin linking,and high purified apoH can be obtained by using the reagent.Polyclonal antibody needs to be further purified before detecting serum apolipoprotein H.

Objective To isolate and identify human anti-oxidized low density lipoprotein (LDL) autoantibodies.Methods Oxidized LDL (Ox-LDL) autoantibodies were isolated from eight healthy subjects by affinity chromatography with using an Ox-LDL cross-linked Sepharose 4B column.Results Three elution peaks of Ox-LDL autoantibodies were washed out from the Ox-LDL column by NaHCO3,acetate and KCNS buffer in turn and collected respectively.Ox-LDL autoantibodies were isolated from all the selected healthy subjects.All the three elution peaks had good reactivity with Ox-LDL.Among them,the elution peaks by KCNS buffer had high protein level and strong reactivity with Ox-LDL.The types of these antibodies included IgG and IgM, and IgG was the main type.Conclusion The isolated Ox-LDL autoantibodies had strong reactivity with Ox-LDL.

Objective To explore the effects of low density lipoprotein(LDL) containing immune complexes(IC) on the mRNA expression of matrix metalloproteinase-1(MMP-1) and matrix metalloproteinase-1(TIMP-1) in U937 cells.Methods U937 cells were incubated with native LDL,oxidized LDL or LDL-IC,respectively.mRNA expression of matrix metalloproteinase-1(MMP-1) and tissue inhibitor of metalloproteinase-1(TIMP-1) in U937 cells were measured by reverse transcriptase polymerase chain reaction(PT-PCR).Results Normal U937 cells hardly expressed MMP-1,but highly expressed TIMP-1.N-LDL had no effect on the expression of MMP-1 and TIMP-1 mRNA.Ox-LDL can induce slightly expression of MMP-1 and markedly down-regulate expression of TIMP-1.Similarly,nature and oxidized LDL-IC had significantly more effect on the mRNA expressions of MMP-1 and TIMP-1 than that of control(P

Objective: To explore the mechanism of high incidence of cardiovascular diseases and the levels of plasma lipids and oxidized lowdensity lipoprotein(Ox-LDL) in patients with rheumatoid arthritis(RA).Methods: Fifty-five patients with RA(24 case of active type,31 non-active) and 60 healthy controls were randomly chosen.Plasma lipids and Ox-LDL levels were studied.All the data were subjected to statistical analysis.Results: Compared with the control,total cholesterol(TC),LDL cholesterol(LDL-C) and apolipoprotein B(apoB) levels were increased in patients with active RA,while TC,triglyceride(TG),LDL-C,apoAⅠ,B and Ox-LDL levels were elevated in those with non-active RA.Furthermore,plasma lipids and Ox-LDL level decreased in the active patients as compared with the non-active ones,while only the change of apoAⅠhad statistical significance.The erythrocyte sedimentation rate was found negatively related with TC,LDL-C,high-density lipoprotein cholesterol(HDL-C) and apoAⅠ,while C-reacting protein was found negatively related with LDL-C and HDL-C.Conclusion: Ox-LDL and plasma lipid levels were changed in RA patients.Inflammation may induce changes of lipoprotein and play an important role in atherosclerosis.

Objective To study the influence of nitric oxide synthase (NOS) inhibitor in a rat model of Parkinson’s disease. Methods Hemi-Parkinsonism rat model was established by stereotaxic 6-hydroxydopamine (6-OHDA) lesions in striatum in which nitric oxide synthase (NOS) was inhibited by L-Nitro-Arginine (L-NNA) and apomorphin-induced rotational behavior was measured. The immunohistochemical staining method was used to observe the change of striatal nNOS-positive neurons and nigral tyrosine hydroxylase(TH)-positive neurons. Results L-NNA dramatically protected 6-OHDA-injected rats against indices of severe injury to the nigrostriatal dopaminergic pathway, including decreases in numbers of TH-positive nigral neurons and rotational behavior. The nNOS-positive neurons showed no changes in numbers. Conclusions These results indicate that NO might mediate, in part, 6-OHDA-induced neurotoxicity and nNOS-positive neurons might resist the 6-OHDA neurotoxicity. NOS inhibitor may play a role in the protection of 6-OHDA neurons.