Objective To observe the effect of depside salt from Salvia Miltiorrhiza on platelet endothelial nitric oxide synthase(eNOS) activity in health adults.Methods Peripheral venous blood was collected,platelets were isolated with gel-filtration chromatography and incubated with eNOS agonist histamine,eNOS inhibitor L-NAME and different concentrations of depside salt from Salvia Miltiorrhiza(0.1,1,10,100,1000 mg/L) for 30 minutes,then eNOS activity was measured as formation of 3H-L-citrulline from 3H-L-arginine.Results(1)Platelet eNOS activity was significantly inhibited after incubated platelets with L-NAME and increased after incubated with histamine(P

Objective To explore the changes of plasma endotoxin and D-lactate during intestinal mucosal barrier injury of acute pancreatitis.Methods Wistar rats were randomly divided into sham-operation(SO)group,and severe acute pancreatitis(SAP)group.Plasma endotoxin and D-lactate were determined at 1,3,6 and 12 hour after operations.Results The plasma endotoxin and D-lactate in SAP group increased obviously with time,and the difference betueen the 2 groups was significante(P

Objective To explore the role of apoptosis in the pathogenesis of acute pancreatitis(AP)and to (investigate) the effect of ligustrazin(LGT) on the apoptosis in acute pancreatitis .Methods Experimental animals were divided into three groups randomly: (1) AP group, (2) comparison group,and (3) therapy group. Pathologic score and TUNEL method were used to detect the pathologic mophology of pancreatic tissue and the pancreatic cell apoptosis index in the 3 groups. Results In the early stage of AP,the majority of cells are apototic and the degree of AP is slight.In the late stage of AP,the majority of cells show necrosis,and the degree of AP is severe.The (induction) of cell apoptosis can lessen the pathologic state of AP. Conclusions The degree of pathologic changes in AP can be lessened by the induction of cell apoptosis,but there is a definte time limit.

Objective To explore the role of inflammatory mediators in the pathogenesis of acute pancreatitis(AP).Methods Observation the relationship betweent the changes in serum TNF and IL-6 and(histopathology) of the pancreas in AP rats.Results(1)Rats in sham operation group survived throughout the experiment.Compared with of sham operation group,in AP group survival time shortened significantly(P

Chin ; surgery ; Humans ; Mandible ; surgery ; Maxillofacial Abnormalities ; surgery ; Osteotomy ; methods ; Osteotomy, Le Fort ; methods ; Tooth Abnormalities ; surgery

Objective To study the advantage and disadvantage of different treatments of unstable distal radial fractures in elderly patients. Methods In the period from August 2005 to January 2010, 55 elderly patients with unstable d istal radial fractures were treated with manual diaplasis and external plaster splint fixation, operation and internal plate fixation, or external fixation. Clinical outcomes were evaluated by Gartland-Werley wrist scoring systems and questionnaire of the disabilities of the Arm, Shoulder and Hand (DASH). We used SPSS13.0 software package for statistical analysis. Results Compared with the manual diaplasis group, the operation and internal plate fixation group or external fixation group was better in Gartland-Werley scores, the differences were statistically significant (P<0.05), but there were no differences in DASH scores among the 3 groups. Conclusions The treatment of unstable distal radial fractures in elderly patients should be chosen based on the characteristics of the fractare and patients desire.

目的观察苁蓉通便汤治疗老年性便秘的临床疗效。方法将 62例老年性便秘患者分为两组。治疗组(32例)内服苁蓉通便汤,对照组(30例)内服麻仁润肠丸。结果治疗组疗效优于对照组(P<0.05)。结论苁蓉通便汤对老年性便秘疗效显著。

Objective To study a simple surgical method for continuous rapid tissue expansion and its clinical application. Methods The operation was accomplished in two-stage procedures. First, expanders were placed in the subcutaneous pocket through interlesional perpendicular short incisions. Continuous expansion by trace syringe pump began from the second day after expander placement. The speed of water injection was set according to patient's algesthesia reaction and vascularity of skin. The second operation was carried out at the third to fifth day after the expansion completed.Results The method was put into clinical practice in 22 cases (16 cases of scar, 2 cases of capillary angioma, and 4 cases of black nevus), and 35 expanders were placed. The speed of water injection was set in 0.2～0.8ml/hr/100ml.The mean time of water injection was 15.3 days. In the process of expansion, all cases were satisfactory except 1 case, in which the expander exposed in the incision. After the second operation, all flaps survived except one showing distal skin necrosis. The result was satisfactory after 3 months to 1 year follow-up. Conclusion The procedure consists of expander placement through interlesional perpendicular short incisions and continuous expansion in advance, which maximally shortens the time of expansion and hospitalization. This expansion method is a simple, safe, reliable, and ideal rapid tissue expansion technique.

Objective To characterize the clinical features and the prognosis of paediatric lobar pneumonia. Methods Clinical data of 45 cases with paediatric lobar pneumonia,such as clinical manifestations,symptom, pathogens and prognosis were retrospectively analyzed.Results Of the 45 patients,45 cases had cough,43 cases with fever,15 cases with pain in the chest,13 cases wtih cutaneous lesions,35 cases wtih antibody of mycoplasma positive, 6 cases wtih Staphylococcus aureus infection,21 cases wtih Streptococcus pneumonia infection,cardiovascular and digestive system were involved besides lung,secondly nervous system involved.After treatment,29 cases were cured, 15 cases improved,1 case transferred other hospital.Conclusion Paediatric lobar pneumonia caused by decreased gradually,but increased by virus and other bacteria.Cases of paediatric pneumonia with fever,cough,pulmonary symptom should be tested by laboratory finding,especially antibody and X ray or CT in order to identify pathogenesis of disease,thus to be treated correctly,dosage and course of antibiotics should be also enough.

Objective To investigate the possible role of mRNA expression of nitric oxide synthase in the abnormal intestinal motility and visceral perception in patients with diarrhea predominant IBS. Methods Anorectal manometry and rectal visceral sensory thresholds were examined in 25 patients with diarrhea predominant IBS and 15 healthy controls by means of electric barostat and perfusion catheter manometer. The quantity of NO was measured using nitrate/nitrite colorimetric assay. The nerve fibers containing NOS in mucosal muscular layer of colon was quantitatively measured with NADPH diaphorase histochemistry assay and computer image analysis. The expression of neuronal nitric oxide synthase (nNOS) mRNA was determined with fluorescence quantitative polymerase chain reaction (FQ PCR). Results Thresholds of first perception, defecation and pain in patients with IBS were significantly lower than those of controls. The motility indexes of sigmoid and rectum were significantly higher than those of normal controls ( P