OBJECTIVEThe approach of this paper is make extracting solution' clarity achieve the request level and reduce the pollution of resinic columns through improved technical procedure. Silkworm dropping have sediment with alcohol extract and acid precipitation, affect the clarify performance quality, and polysaccharide and protein in extracting solution is badly for resinic columns in purification process.

METHODAdded freeze-thaw pretreatment in clarified process and researched three thaw methods effect on clarify performance of extracting solution with silkworm dropping. The adding effects were evaluated by content of polysaccharide and protein, transmittance and the yield of active ingredients during this adding process.

RESULTThrough comparing researches of three thaw methods, the microwave thaw method was the best approach.

CONCLUSIONAdding freeze-thaw pretreatment in clarify process is not only can improve the clarity of extracting solution with silkworm dropping on ideal level, reduce pollution of resinic columns, but also remain ideally the active ingredients.

Animals ; Biological Factors ; isolation & purification ; Bombyx ; chemistry ; Chemical Fractionation ; instrumentation ; methods ; Feces ; chemistry ; Freezing