2.Detection and Analysis of Integron and Metallo-?-Lactamase in Stenotrophomonas maltophilia
Huaimin XIONG ; Chuandan WAN ; Jianhua SHENG ; Chen WU ; Yinghong CUI
Chinese Journal of Nosocomiology 2006;0(06):-
OBJECTIVE To study the existence of integron and metallo-?-lactamase in Stenotrophomonas maltophilia in our hospital.METHODS The antibiotic susceptibility was tested by K-B method.The genomic DNA and their plasmids were extracted.The integron and metallo-?-lactamase gene were amplified by polymerase chain reaction(PCR).RESULTS L1 and L2 genes were amplified in chromosomes and plasmids.Integrons Ⅰ and Ⅱ were detected in genomic DNA.CONCLUSIONS Existence of metallo-?-lactamase is one of reasons of multi-drug resistance in S.maltophilia.S.maltophilia can receive integrons and gain its multi-drug resistance from other strains.
3.Detecting Results of HBV-DNA,HBeAg and PreS1 of HBV Infected Sera:Analysis and Significance
Liya CHEN ; Sheng ZHAO ; Maofeng WANG ; Rugen WAN
Chinese Journal of Nosocomiology 2009;0(17):-
OBJECTIVE To detect and analyze the results and significance of HBV-DNA,HBeAg and PreS1 of HBV infective sera.METHODS Routine detection and PreS1 antigen of 450 sera were tested by ELISA,and HBV-DNA was detected by fluorescent quantitation PCR.RESULTS The positive rates of HBV-DNA,HBeAg and PreS1 were 74.4%,48.9% and 63.3%,respectively,in 450 cases of HBV infective sera.Among 285 PreS1-positive samples,the positive cases of HBeAg and HBV-DNA were 190 and 270,respectively.There were significant difference(P0.05)among PreS1,HBV-DNA and PreS1,and they three were in positive correlation.CONCLUSIONS PreS1 and HBV-DNA are more sensitive than HBeAg,and PreS1 is better coincided with HBV-DNA.They can reflect the infection and replication condition of HBV.Therefore,it has important clinical meaning for the diagnosis and therapy of HBV to simultaneously used HBV-DNA,HBeAg and PreS1.
4.High-risk HPV DNA and Early Cervical Dysplasia and Cervical Carcinoma:Their Related Analysis
Liya CHEN ; Sheng ZHAO ; Yongjun ZHANG ; Guogang LI ; Rugen WAN
Chinese Journal of Nosocomiology 2009;0(24):-
0.05).While there were significant differences when comparing CINⅠwith CINⅡ-Ⅲ(?2=14.97,P
5.Surgical treatment of 280 spontaneous hemopneumothorax patients.
Sheng-xi CHEN ; Wei GONG ; Wan-jun LUO
Journal of Central South University(Medical Sciences) 2005;30(3):363-364
Adolescent
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Adult
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Aged
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Child
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Child, Preschool
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Female
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Hemopneumothorax
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surgery
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Humans
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Infant
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Male
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Middle Aged
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Paracentesis
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Thoracoscopy
6.Expression of survivin E-cadherin and Ki-67 in pituitary adenoma and correlation with invasiveness.
Chun-Liang WANG ; Jin-Hong MEI ; Hong-Ping WAN ; Ren-Sheng CHEN ; Lin-Lin XU
Chinese Journal of Pathology 2008;37(5):335-336
Adolescent
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Adult
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Aged
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Cadherins
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genetics
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metabolism
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physiology
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Female
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Humans
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Inhibitor of Apoptosis Proteins
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Ki-67 Antigen
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genetics
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metabolism
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Male
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Microtubule-Associated Proteins
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genetics
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metabolism
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physiology
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Middle Aged
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Neoplasm Invasiveness
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physiopathology
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Pituitary Neoplasms
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pathology
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physiopathology
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Young Adult
7.Investigation and analysis of effect of teaching competition on young teachers' teaching ability
Baojun WAN ; Jiagao LV ; Zhuo CHEN ; Tao SHU ; Fei TAN ; Junyue ZHANG ; Sheng WEI
Chinese Journal of Medical Education Research 2013;(10):1064-1067
Objective To investigate current situation of teaching competition and to put forward some feasible suggestions for improving the teaching competition of theoretical course for young teachers. Methods Questionnaire survey was conducted for 126 teachers including the effect of teaching competi-tion on effective teaching atmosphere,comprehensive teaching results,teaching ability and recommenda-tions for improvement. SPSS software was used to do statistical analysis andχ22 test was applied. P<0 . 05 stands for statistically significant difference. Results Investigation results demonstrated that teaching competition of theoretical course was conducive to forming effective teaching atmosphere,achieving better teaching effect and improving teaching ability of teachers . A χ22 test was performed on the cognition of the cultivating of classroom management ability;results demonstrated that there were significant differ-ences between teachers with more than 10 years education age and those with less than 10 years education age(χ22=4.375,P=0.0365). Conclusions Teaching competition of theoretical course is an effective way to cultivate young teachers teaching ability. Teaching competition should be combined with training and feedback and evaluation should be more scientific and objective in order to improve young teachers' teaching ability.
8.Extrapleural solitary fibrous tumor: report of a case.
Jin-hong MEI ; Hong-ping WAN ; Han LIU ; Ren-sheng CHEN ; Hui-chun GAO ; Shan XU
Chinese Journal of Pathology 2006;35(8):510-511
9.Analysis of risk factors in patients with trauma accompanied by multiple organ dysfunction syndrome
Wei ZHU ; Hua-Wen CHEN ; Rui TANG ; Lei WAN ; Qiang ZHONG ; Shu-Sheng LI ;
Chinese Journal of Emergency Medicine 2006;0(12):-
Objective To study the risk factors in patients with trauma accompanied by multiple organ dysfunction syndrome.Method The data of 107 patients with trauma in ICU,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,were retrospectively studied.All patients were divided into 2 groups:MODS group and non-MODS group.The clinical and laboratory,results,ISS score,APACHEⅢscore and GCS score were studied.Results There were no differences on gender,age and mobility of shock between the two groups.There were significant differences on the treatment of shock,ISS score,APACHEⅢscore,CCS score,the levels of blood sugar and platelet between two groups.The Logistic regression analysis showed the main risk factors were treatment of shock,ISS score and APACHEⅢscore.Conclusions The risk factors in patients with trauma accompanied by multiple organ dysfunction syndrome were the treatment of shock, ISS score and APACHEⅢscore.
10.Aortic Valvuloplasty in Treatment of Ventricular Septal Defect with Aortic Valve Insufficiency in Children
xiang, LING ; sheng-xi, CHEN ; wan-jun, LUO ; ling-jin, HUANG
Journal of Applied Clinical Pediatrics 2006;0(21):-
Objective To explore the clinical effects of aortic valvuloplasty in treatment of ventricular septal defect(VSD)with moderate or severe aortic valve insufficiency(AI)in children.Methods Thirteen children less than 14 years old underwent open heart surgery as well as aortic valvuloplasty for VSD with moderate or severe AI.There were 9 cases of subpulmonary VSD,perimenbranous VSD in 4 cases.AI was mo-derate in 4 cases,severe in 9 cases.Results There was no hospital death.Post-operative echocardiogram and X-ray revealed that both cardiothoracic ratio and left ventricular end diastolic diameter decreased markedly(P